Paracoccus siganidrum
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4665 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A418ZT36|A0A418ZT36_9RHOB Methylamine utilization protein MauF (Fragment) OS=Paracoccus siganidrum OX=1276757 GN=D3P05_23105 PE=4 SV=1
MM1 pKa = 7.51 GAGSDD6 pKa = 3.89 PVCAGNARR14 pKa = 11.84 KK15 pKa = 9.7 RR16 pKa = 11.84 KK17 pKa = 7.98 EE18 pKa = 3.88 LEE20 pKa = 3.86 MPIGTNFNDD29 pKa = 3.91 TLIGDD34 pKa = 3.76 NTAEE38 pKa = 4.3 LFFGLGGNDD47 pKa = 4.43 LILGNGGNDD56 pKa = 3.61 TIYY59 pKa = 11.06 GGTGNDD65 pKa = 3.62 TVDD68 pKa = 3.45 GGSGNDD74 pKa = 4.11 LIYY77 pKa = 11.27 GEE79 pKa = 5.22 AGNDD83 pKa = 3.57 SLVGGGGTDD92 pKa = 3.6 TIYY95 pKa = 11.02 GGEE98 pKa = 4.2 GNDD101 pKa = 4.08 TIRR104 pKa = 11.84 SSGAGFYY111 pKa = 10.9 DD112 pKa = 3.87 GGNGNDD118 pKa = 3.43 YY119 pKa = 10.88 VYY121 pKa = 11.06 AGLGVAEE128 pKa = 4.45 TLRR131 pKa = 11.84 GGAGVDD137 pKa = 3.35 WLNTTNWNSAYY148 pKa = 10.44 VINLATGLTNFAGEE162 pKa = 4.4 SFTQFEE168 pKa = 4.46 NLVTGNGNDD177 pKa = 3.87 SLTGTADD184 pKa = 3.05 ANTIYY189 pKa = 10.72 SGGGDD194 pKa = 3.17 DD195 pKa = 3.96 TVRR198 pKa = 11.84 GEE200 pKa = 4.41 AGNDD204 pKa = 3.5 YY205 pKa = 10.73 ISAGGGNDD213 pKa = 3.65 LLYY216 pKa = 10.93 GGSGNDD222 pKa = 3.33 TVLGGDD228 pKa = 3.82 GNDD231 pKa = 3.21 RR232 pKa = 11.84 LYY234 pKa = 11.54 GEE236 pKa = 5.11 AGNDD240 pKa = 3.53 YY241 pKa = 10.99 LYY243 pKa = 11.49 GDD245 pKa = 5.01 AGNDD249 pKa = 3.88 TIDD252 pKa = 3.72 GGSGNDD258 pKa = 3.61 YY259 pKa = 11.09 LSGGSGNDD267 pKa = 3.2 SLTGGGGTDD276 pKa = 3.65 TIYY279 pKa = 11.02 GGEE282 pKa = 4.2 GNDD285 pKa = 4.08 TIRR288 pKa = 11.84 SSGAGVYY295 pKa = 10.18 DD296 pKa = 3.93 GQAGDD301 pKa = 3.64 DD302 pKa = 3.6 HH303 pKa = 8.75 VYY305 pKa = 11.08 AGLGVAEE312 pKa = 4.45 TLRR315 pKa = 11.84 GGAGVDD321 pKa = 3.07 WLDD324 pKa = 3.26 TTTYY328 pKa = 9.93 GGNYY332 pKa = 9.35 QVDD335 pKa = 4.23 LASGLTNYY343 pKa = 10.31 AGEE346 pKa = 4.32 SFVQFDD352 pKa = 4.5 HH353 pKa = 6.45 LQSGQGHH360 pKa = 6.25 DD361 pKa = 3.95 TLFGTAGDD369 pKa = 3.75 NQIRR373 pKa = 11.84 GGGGADD379 pKa = 3.86 RR380 pKa = 11.84 IHH382 pKa = 6.52 GRR384 pKa = 11.84 AGHH387 pKa = 6.97 DD388 pKa = 3.9 LLNGQAGHH396 pKa = 6.92 DD397 pKa = 3.78 TLNGNNGNDD406 pKa = 3.71 TLLGQAGNDD415 pKa = 3.69 VLNGGAGQDD424 pKa = 3.75 VLRR427 pKa = 11.84 GGAGSDD433 pKa = 3.18 ILSGQQGHH441 pKa = 6.41 DD442 pKa = 3.4 QLFGEE447 pKa = 5.16 AGPDD451 pKa = 3.43 QLLGGAGNDD460 pKa = 3.89 TLNGGAGHH468 pKa = 6.92 DD469 pKa = 4.11 TLNGGAGNDD478 pKa = 4.03 LLIGGAGNDD487 pKa = 3.82 LLIGGGGEE495 pKa = 3.86 DD496 pKa = 3.16 TFRR499 pKa = 11.84 FVSISDD505 pKa = 3.75 SPSGGNHH512 pKa = 5.39 DD513 pKa = 4.85 TISGFQGAGNRR524 pKa = 11.84 PAAAIEE530 pKa = 4.0 DD531 pKa = 4.91 LIDD534 pKa = 5.08 LSQIDD539 pKa = 4.37 ANTLVGGNQAFTFNGTTPGGVGTIWLQNVGNATWLHH575 pKa = 6.15 LNVDD579 pKa = 3.42 NDD581 pKa = 3.96 AAPEE585 pKa = 3.91 MTIRR589 pKa = 11.84 ILDD592 pKa = 3.95 GATTAGDD599 pKa = 3.44 YY600 pKa = 8.55 WAGDD604 pKa = 4.14 FILL607 pKa = 5.43
Molecular weight: 60.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.605
IPC_protein 3.668
Toseland 3.414
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.643
Rodwell 3.478
Grimsley 3.325
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 4.101
Thurlkill 3.478
EMBOSS 3.656
Sillero 3.795
Patrickios 0.846
IPC_peptide 3.668
IPC2_peptide 3.757
IPC2.peptide.svr19 3.706
Protein with the highest isoelectric point:
>tr|A0A419A9J6|A0A419A9J6_9RHOB DUF3445 domain-containing protein OS=Paracoccus siganidrum OX=1276757 GN=D3P05_05035 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.12 GGRR28 pKa = 11.84 RR29 pKa = 11.84 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.69 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4665
0
4665
1441716
28
4715
309.0
33.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.43 ± 0.057
0.85 ± 0.012
5.89 ± 0.031
5.665 ± 0.031
3.507 ± 0.022
9.15 ± 0.039
2.117 ± 0.017
5.066 ± 0.023
2.276 ± 0.029
10.475 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.7 ± 0.017
2.25 ± 0.021
5.566 ± 0.029
3.26 ± 0.02
7.818 ± 0.039
4.745 ± 0.023
4.881 ± 0.026
6.916 ± 0.026
1.466 ± 0.016
1.974 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here