Novosphingobium sp. GV013

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3746 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R7DES9|A0A4R7DES9_9SPHN Uncharacterized protein DUF1153 OS=Novosphingobium sp. GV013 OX=2135688 GN=C8K09_2222 PE=4 SV=1
MM1 pKa = 6.5TAKK4 pKa = 10.68LIKK7 pKa = 10.09LTDD10 pKa = 3.85RR11 pKa = 11.84YY12 pKa = 10.54IEE14 pKa = 4.01TCIDD18 pKa = 3.81DD19 pKa = 4.54EE20 pKa = 4.77VVLMDD25 pKa = 5.1LDD27 pKa = 3.97SGNFFSLTGTAATIWAMIDD46 pKa = 3.41DD47 pKa = 4.34VRR49 pKa = 11.84DD50 pKa = 3.46SEE52 pKa = 5.76AIIACACTEE61 pKa = 4.25YY62 pKa = 11.27GCGAAEE68 pKa = 4.05IAGDD72 pKa = 3.7VSDD75 pKa = 5.94FLQQMTEE82 pKa = 3.62AGFIAARR89 pKa = 3.8

Molecular weight: 9.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R7DEM9|A0A4R7DEM9_9SPHN Sugar transferase OS=Novosphingobium sp. GV013 OX=2135688 GN=C8K09_1997 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.32GFRR19 pKa = 11.84TRR21 pKa = 11.84SATPGGRR28 pKa = 11.84KK29 pKa = 8.09VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3746

0

3746

1231242

25

4431

328.7

35.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.52 ± 0.058

0.841 ± 0.014

5.897 ± 0.033

5.063 ± 0.035

3.523 ± 0.024

8.999 ± 0.051

2.051 ± 0.019

4.94 ± 0.028

2.726 ± 0.029

9.803 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.02

2.525 ± 0.022

5.439 ± 0.035

3.09 ± 0.021

7.365 ± 0.046

5.259 ± 0.036

5.451 ± 0.042

7.433 ± 0.029

1.41 ± 0.019

2.121 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski