Amino acid dipepetide frequency for Novosphingobium sp. GV013

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.081AlaAla: 19.081 ± 0.202	1.144AlaCys: 1.144 ± 0.035	7.643AlaAsp: 7.643 ± 0.089	7.08AlaGlu: 7.08 ± 0.084	4.384AlaPhe: 4.384 ± 0.073	11.85AlaGly: 11.85 ± 0.13	2.492AlaHis: 2.492 ± 0.043	7.002AlaIle: 7.002 ± 0.083	3.496AlaLys: 3.496 ± 0.069	14.299AlaLeu: 14.299 ± 0.134	4.465AlaMet: 4.465 ± 0.068	3.084AlaAsn: 3.084 ± 0.053	6.681AlaPro: 6.681 ± 0.093	4.745AlaGln: 4.745 ± 0.075	10.58AlaArg: 10.58 ± 0.119	6.741AlaSer: 6.741 ± 0.078	7.237AlaThr: 7.237 ± 0.081	9.096AlaVal: 9.096 ± 0.099	1.688AlaTrp: 1.688 ± 0.042	2.419AlaTyr: 2.419 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	1.096CysAla: 1.096 ± 0.036	0.106CysCys: 0.106 ± 0.009	0.573CysAsp: 0.573 ± 0.022	0.423CysGlu: 0.423 ± 0.022	0.28CysPhe: 0.28 ± 0.019	0.908CysGly: 0.908 ± 0.03	0.256CysHis: 0.256 ± 0.019	0.364CysIle: 0.364 ± 0.018	0.176CysLys: 0.176 ± 0.012	0.76CysLeu: 0.76 ± 0.026	0.184CysMet: 0.184 ± 0.012	0.227CysAsn: 0.227 ± 0.013	0.481CysPro: 0.481 ± 0.019	0.22CysGln: 0.22 ± 0.013	0.553CysArg: 0.553 ± 0.023	0.463CysSer: 0.463 ± 0.021	0.42CysThr: 0.42 ± 0.018	0.609CysVal: 0.609 ± 0.022	0.132CysTrp: 0.132 ± 0.01	0.181CysTyr: 0.181 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.612AspAla: 7.612 ± 0.085	0.508AspCys: 0.508 ± 0.02	3.271AspAsp: 3.271 ± 0.059	3.259AspGlu: 3.259 ± 0.061	2.079AspPhe: 2.079 ± 0.043	5.648AspGly: 5.648 ± 0.077	1.423AspHis: 1.423 ± 0.041	2.718AspIle: 2.718 ± 0.052	1.667AspLys: 1.667 ± 0.039	5.801AspLeu: 5.801 ± 0.077	1.389AspMet: 1.389 ± 0.037	1.34AspAsn: 1.34 ± 0.033	3.913AspPro: 3.913 ± 0.056	1.685AspGln: 1.685 ± 0.039	4.896AspArg: 4.896 ± 0.074	2.431AspSer: 2.431 ± 0.053	2.644AspThr: 2.644 ± 0.057	4.2AspVal: 4.2 ± 0.058	1.039AspTrp: 1.039 ± 0.032	1.444AspTyr: 1.444 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	7.103GluAla: 7.103 ± 0.098	0.368GluCys: 0.368 ± 0.018	2.501GluAsp: 2.501 ± 0.05	2.528GluGlu: 2.528 ± 0.062	1.598GluPhe: 1.598 ± 0.041	4.144GluGly: 4.144 ± 0.058	1.055GluHis: 1.055 ± 0.033	2.736GluIle: 2.736 ± 0.056	1.687GluLys: 1.687 ± 0.042	4.731GluLeu: 4.731 ± 0.071	1.399GluMet: 1.399 ± 0.033	1.256GluAsn: 1.256 ± 0.032	2.454GluPro: 2.454 ± 0.048	2.062GluGln: 2.062 ± 0.042	4.594GluArg: 4.594 ± 0.076	2.281GluSer: 2.281 ± 0.044	2.888GluThr: 2.888 ± 0.052	3.531GluVal: 3.531 ± 0.064	0.784GluTrp: 0.784 ± 0.026	0.934GluTyr: 0.934 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	5.033PheAla: 5.033 ± 0.066	0.338PheCys: 0.338 ± 0.017	2.701PheAsp: 2.701 ± 0.049	1.888PheGlu: 1.888 ± 0.043	1.208PhePhe: 1.208 ± 0.037	3.733PheGly: 3.733 ± 0.062	0.75PheHis: 0.75 ± 0.028	1.379PheIle: 1.379 ± 0.035	0.803PheLys: 0.803 ± 0.026	2.973PheLeu: 2.973 ± 0.056	0.724PheMet: 0.724 ± 0.021	1.014PheAsn: 1.014 ± 0.029	1.573PhePro: 1.573 ± 0.038	0.867PheGln: 0.867 ± 0.026	2.182PheArg: 2.182 ± 0.043	2.043PheSer: 2.043 ± 0.042	1.993PheThr: 1.993 ± 0.042	2.684PheVal: 2.684 ± 0.045	0.482PheTrp: 0.482 ± 0.021	0.858PheTyr: 0.858 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	10.406GlyAla: 10.406 ± 0.113	0.872GlyCys: 0.872 ± 0.029	4.839GlyAsp: 4.839 ± 0.076	4.59GlyGlu: 4.59 ± 0.066	3.518GlyPhe: 3.518 ± 0.067	8.389GlyGly: 8.389 ± 0.162	2.011GlyHis: 2.011 ± 0.042	4.469GlyIle: 4.469 ± 0.064	3.241GlyLys: 3.241 ± 0.061	8.832GlyLeu: 8.832 ± 0.095	2.521GlyMet: 2.521 ± 0.052	2.404GlyAsn: 2.404 ± 0.065	3.88GlyPro: 3.88 ± 0.062	3.111GlyGln: 3.111 ± 0.052	6.361GlyArg: 6.361 ± 0.078	4.967GlySer: 4.967 ± 0.098	5.306GlyThr: 5.306 ± 0.107	6.302GlyVal: 6.302 ± 0.08	1.657GlyTrp: 1.657 ± 0.042	2.312GlyTyr: 2.312 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	2.669HisAla: 2.669 ± 0.049	0.221HisCys: 0.221 ± 0.014	1.283HisAsp: 1.283 ± 0.034	1.04HisGlu: 1.04 ± 0.031	0.83HisPhe: 0.83 ± 0.025	2.125HisGly: 2.125 ± 0.045	0.556HisHis: 0.556 ± 0.022	0.871HisIle: 0.871 ± 0.028	0.433HisLys: 0.433 ± 0.019	1.907HisLeu: 1.907 ± 0.038	0.449HisMet: 0.449 ± 0.019	0.478HisAsn: 0.478 ± 0.021	1.377HisPro: 1.377 ± 0.034	0.505HisGln: 0.505 ± 0.021	1.563HisArg: 1.563 ± 0.036	0.927HisSer: 0.927 ± 0.03	0.77HisThr: 0.77 ± 0.028	1.62HisVal: 1.62 ± 0.037	0.343HisTrp: 0.343 ± 0.018	0.54HisTyr: 0.54 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	8.015IleAla: 8.015 ± 0.097	0.437IleCys: 0.437 ± 0.021	3.99IleAsp: 3.99 ± 0.058	3.376IleGlu: 3.376 ± 0.063	1.38IlePhe: 1.38 ± 0.036	4.942IleGly: 4.942 ± 0.074	0.905IleHis: 0.905 ± 0.028	1.881IleIle: 1.881 ± 0.052	1.216IleLys: 1.216 ± 0.033	3.609IleLeu: 3.609 ± 0.06	0.958IleMet: 0.958 ± 0.031	1.38IleAsn: 1.38 ± 0.039	2.29IlePro: 2.29 ± 0.048	1.142IleGln: 1.142 ± 0.031	3.11IleArg: 3.11 ± 0.053	2.554IleSer: 2.554 ± 0.055	2.565IleThr: 2.565 ± 0.052	4.164IleVal: 4.164 ± 0.062	0.533IleTrp: 0.533 ± 0.023	0.955IleTyr: 0.955 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	3.785LysAla: 3.785 ± 0.065	0.147LysCys: 0.147 ± 0.011	1.393LysAsp: 1.393 ± 0.037	1.053LysGlu: 1.053 ± 0.031	0.835LysPhe: 0.835 ± 0.026	2.514LysGly: 2.514 ± 0.053	0.475LysHis: 0.475 ± 0.021	1.267LysIle: 1.267 ± 0.036	0.894LysLys: 0.894 ± 0.034	2.831LysLeu: 2.831 ± 0.051	0.647LysMet: 0.647 ± 0.02	0.645LysAsn: 0.645 ± 0.025	1.84LysPro: 1.84 ± 0.043	0.796LysGln: 0.796 ± 0.027	1.925LysArg: 1.925 ± 0.044	1.484LysSer: 1.484 ± 0.038	1.487LysThr: 1.487 ± 0.036	2.305LysVal: 2.305 ± 0.05	0.383LysTrp: 0.383 ± 0.015	0.552LysTyr: 0.552 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	14.734LeuAla: 14.734 ± 0.133	0.888LeuCys: 0.888 ± 0.026	6.14LeuAsp: 6.14 ± 0.077	4.424LeuGlu: 4.424 ± 0.072	3.475LeuPhe: 3.475 ± 0.057	8.37LeuGly: 8.37 ± 0.094	1.853LeuHis: 1.853 ± 0.038	4.569LeuIle: 4.569 ± 0.07	2.707LeuLys: 2.707 ± 0.05	9.14LeuLeu: 9.14 ± 0.125	2.152LeuMet: 2.152 ± 0.044	2.38LeuAsn: 2.38 ± 0.05	5.615LeuPro: 5.615 ± 0.076	2.493LeuGln: 2.493 ± 0.05	6.957LeuArg: 6.957 ± 0.08	6.066LeuSer: 6.066 ± 0.073	5.585LeuThr: 5.585 ± 0.075	7.373LeuVal: 7.373 ± 0.095	1.194LeuTrp: 1.194 ± 0.036	1.911LeuTyr: 1.911 ± 0.038	0.0LeuXaa: 0.0 ± 0.0
Met	3.769MetAla: 3.769 ± 0.057	0.149MetCys: 0.149 ± 0.012	1.18MetAsp: 1.18 ± 0.031	1.038MetGlu: 1.038 ± 0.033	0.709MetPhe: 0.709 ± 0.025	2.011MetGly: 2.011 ± 0.046	0.434MetHis: 0.434 ± 0.016	1.41MetIle: 1.41 ± 0.033	0.884MetLys: 0.884 ± 0.031	2.663MetLeu: 2.663 ± 0.05	0.7MetMet: 0.7 ± 0.026	0.771MetAsn: 0.771 ± 0.026	1.596MetPro: 1.596 ± 0.039	0.733MetGln: 0.733 ± 0.026	1.832MetArg: 1.832 ± 0.04	1.409MetSer: 1.409 ± 0.033	1.763MetThr: 1.763 ± 0.043	1.909MetVal: 1.909 ± 0.043	0.228MetTrp: 0.228 ± 0.014	0.256MetTyr: 0.256 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.342AsnAla: 3.342 ± 0.063	0.248AsnCys: 0.248 ± 0.016	1.388AsnAsp: 1.388 ± 0.033	1.054AsnGlu: 1.054 ± 0.032	0.964AsnPhe: 0.964 ± 0.033	2.486AsnGly: 2.486 ± 0.059	0.497AsnHis: 0.497 ± 0.021	1.247AsnIle: 1.247 ± 0.036	0.597AsnLys: 0.597 ± 0.023	2.436AsnLeu: 2.436 ± 0.047	0.499AsnMet: 0.499 ± 0.022	0.647AsnAsn: 0.647 ± 0.026	1.846AsnPro: 1.846 ± 0.039	0.704AsnGln: 0.704 ± 0.028	1.836AsnArg: 1.836 ± 0.043	1.227AsnSer: 1.227 ± 0.038	1.214AsnThr: 1.214 ± 0.042	1.906AsnVal: 1.906 ± 0.045	0.396AsnTrp: 0.396 ± 0.018	0.716AsnTyr: 0.716 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	7.635ProAla: 7.635 ± 0.1	0.372ProCys: 0.372 ± 0.017	3.857ProAsp: 3.857 ± 0.061	3.248ProGlu: 3.248 ± 0.049	1.947ProPhe: 1.947 ± 0.038	5.109ProGly: 5.109 ± 0.07	1.101ProHis: 1.101 ± 0.031	2.353ProIle: 2.353 ± 0.047	1.254ProLys: 1.254 ± 0.038	5.04ProLeu: 5.04 ± 0.071	1.328ProMet: 1.328 ± 0.032	1.165ProAsn: 1.165 ± 0.03	2.896ProPro: 2.896 ± 0.063	1.751ProGln: 1.751 ± 0.037	3.354ProArg: 3.354 ± 0.057	2.833ProSer: 2.833 ± 0.055	2.626ProThr: 2.626 ± 0.051	4.712ProVal: 4.712 ± 0.063	0.741ProTrp: 0.741 ± 0.027	1.067ProTyr: 1.067 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	4.237GlnAla: 4.237 ± 0.065	0.233GlnCys: 0.233 ± 0.014	1.447GlnAsp: 1.447 ± 0.031	1.206GlnGlu: 1.206 ± 0.031	1.143GlnPhe: 1.143 ± 0.03	2.593GlnGly: 2.593 ± 0.051	0.612GlnHis: 0.612 ± 0.022	1.689GlnIle: 1.689 ± 0.042	0.814GlnLys: 0.814 ± 0.03	2.977GlnLeu: 2.977 ± 0.049	0.791GlnMet: 0.791 ± 0.026	0.732GlnAsn: 0.732 ± 0.026	1.801GlnPro: 1.801 ± 0.037	1.201GlnGln: 1.201 ± 0.035	2.387GlnArg: 2.387 ± 0.053	1.789GlnSer: 1.789 ± 0.042	1.651GlnThr: 1.651 ± 0.038	2.502GlnVal: 2.502 ± 0.049	0.462GlnTrp: 0.462 ± 0.021	0.634GlnTyr: 0.634 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	9.093ArgAla: 9.093 ± 0.105	0.551ArgCys: 0.551 ± 0.022	4.341ArgAsp: 4.341 ± 0.065	3.912ArgGlu: 3.912 ± 0.066	2.991ArgPhe: 2.991 ± 0.052	5.088ArgGly: 5.088 ± 0.065	1.789ArgHis: 1.789 ± 0.042	4.262ArgIle: 4.262 ± 0.067	2.172ArgLys: 2.172 ± 0.042	8.067ArgLeu: 8.067 ± 0.1	2.04ArgMet: 2.04 ± 0.045	1.934ArgAsn: 1.934 ± 0.038	3.84ArgPro: 3.84 ± 0.057	2.531ArgGln: 2.531 ± 0.043	5.739ArgArg: 5.739 ± 0.089	3.691ArgSer: 3.691 ± 0.064	3.791ArgThr: 3.791 ± 0.056	4.866ArgVal: 4.866 ± 0.064	1.178ArgTrp: 1.178 ± 0.034	1.773ArgTyr: 1.773 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	6.634SerAla: 6.634 ± 0.092	0.452SerCys: 0.452 ± 0.018	3.094SerAsp: 3.094 ± 0.061	2.381SerGlu: 2.381 ± 0.047	2.051SerPhe: 2.051 ± 0.042	5.544SerGly: 5.544 ± 0.097	1.011SerHis: 1.011 ± 0.033	2.659SerIle: 2.659 ± 0.052	1.207SerLys: 1.207 ± 0.029	5.226SerLeu: 5.226 ± 0.072	1.236SerMet: 1.236 ± 0.029	1.334SerAsn: 1.334 ± 0.038	2.943SerPro: 2.943 ± 0.053	1.541SerGln: 1.541 ± 0.034	3.694SerArg: 3.694 ± 0.062	2.873SerSer: 2.873 ± 0.065	2.857SerThr: 2.857 ± 0.054	3.777SerVal: 3.777 ± 0.06	0.754SerTrp: 0.754 ± 0.026	1.319SerTyr: 1.319 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	6.804ThrAla: 6.804 ± 0.079	0.447ThrCys: 0.447 ± 0.02	2.777ThrAsp: 2.777 ± 0.05	2.297ThrGlu: 2.297 ± 0.041	1.979ThrPhe: 1.979 ± 0.048	5.732ThrGly: 5.732 ± 0.104	0.998ThrHis: 0.998 ± 0.031	3.098ThrIle: 3.098 ± 0.058	1.126ThrLys: 1.126 ± 0.035	5.81ThrLeu: 5.81 ± 0.082	1.368ThrMet: 1.368 ± 0.033	1.415ThrAsn: 1.415 ± 0.046	3.368ThrPro: 3.368 ± 0.051	1.515ThrGln: 1.515 ± 0.039	3.534ThrArg: 3.534 ± 0.051	2.732ThrSer: 2.732 ± 0.056	3.059ThrThr: 3.059 ± 0.066	4.596ThrVal: 4.596 ± 0.084	0.635ThrTrp: 0.635 ± 0.022	1.217ThrTyr: 1.217 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	10.173ValAla: 10.173 ± 0.098	0.588ValCys: 0.588 ± 0.02	4.348ValAsp: 4.348 ± 0.059	4.274ValGlu: 4.274 ± 0.065	2.418ValPhe: 2.418 ± 0.049	5.818ValGly: 5.818 ± 0.085	1.385ValHis: 1.385 ± 0.036	3.886ValIle: 3.886 ± 0.059	1.866ValLys: 1.866 ± 0.042	7.142ValLeu: 7.142 ± 0.069	1.827ValMet: 1.827 ± 0.038	1.973ValAsn: 1.973 ± 0.047	4.197ValPro: 4.197 ± 0.065	2.041ValGln: 2.041 ± 0.042	5.387ValArg: 5.387 ± 0.07	4.091ValSer: 4.091 ± 0.062	4.83ValThr: 4.83 ± 0.073	5.905ValVal: 5.905 ± 0.085	0.897ValTrp: 0.897 ± 0.027	1.289ValTyr: 1.289 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.394TrpAla: 1.394 ± 0.034	0.123TrpCys: 0.123 ± 0.009	0.736TrpAsp: 0.736 ± 0.027	0.532TrpGlu: 0.532 ± 0.023	0.569TrpPhe: 0.569 ± 0.024	0.923TrpGly: 0.923 ± 0.028	0.391TrpHis: 0.391 ± 0.017	0.729TrpIle: 0.729 ± 0.024	0.473TrpLys: 0.473 ± 0.019	1.751TrpLeu: 1.751 ± 0.041	0.358TrpMet: 0.358 ± 0.016	0.442TrpAsn: 0.442 ± 0.021	0.724TrpPro: 0.724 ± 0.026	0.612TrpGln: 0.612 ± 0.024	1.329TrpArg: 1.329 ± 0.032	0.851TrpSer: 0.851 ± 0.034	0.789TrpThr: 0.789 ± 0.027	0.844TrpVal: 0.844 ± 0.025	0.25TrpTrp: 0.25 ± 0.014	0.277TrpTyr: 0.277 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.589TyrAla: 2.589 ± 0.05	0.221TyrCys: 0.221 ± 0.013	1.464TyrAsp: 1.464 ± 0.04	1.031TyrGlu: 1.031 ± 0.032	0.867TyrPhe: 0.867 ± 0.03	2.063TyrGly: 2.063 ± 0.05	0.512TyrHis: 0.512 ± 0.019	0.813TyrIle: 0.813 ± 0.027	0.543TyrLys: 0.543 ± 0.021	2.034TyrLeu: 2.034 ± 0.047	0.411TyrMet: 0.411 ± 0.018	0.633TyrAsn: 0.633 ± 0.025	1.075TyrPro: 1.075 ± 0.032	0.647TyrGln: 0.647 ± 0.022	1.835TyrArg: 1.835 ± 0.043	1.132TyrSer: 1.132 ± 0.037	1.031TyrThr: 1.031 ± 0.031	1.435TyrVal: 1.435 ± 0.034	0.321TyrTrp: 0.321 ± 0.017	0.556TyrTyr: 0.556 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3746 proteins (1231242 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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