Bovine ephemeral fever virus (strain BB7721) (BEFV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Ephemerovirus; Bovine fever ephemerovirus; Bovine ephemeral fever virus

Average proteome isoelectric point is 7.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9E787|PHOSP_BEFVB Phosphoprotein OS=Bovine ephemeral fever virus (strain BB7721) OX=928297 GN=P PE=3 SV=1
MM1 pKa = 7.31SHH3 pKa = 7.47LKK5 pKa = 9.92PKK7 pKa = 10.41IMTGAYY13 pKa = 9.67DD14 pKa = 3.52AEE16 pKa = 4.21KK17 pKa = 10.59LRR19 pKa = 11.84RR20 pKa = 11.84NLQEE24 pKa = 3.96QIALEE29 pKa = 4.09EE30 pKa = 4.8DD31 pKa = 3.4EE32 pKa = 6.26LEE34 pKa = 4.28NQTDD38 pKa = 3.93FEE40 pKa = 5.33SNKK43 pKa = 10.3EE44 pKa = 3.7EE45 pKa = 4.87SNINNNPLVIKK56 pKa = 9.05QEE58 pKa = 3.76PSIYY62 pKa = 9.88PIEE65 pKa = 4.72INLEE69 pKa = 4.08DD70 pKa = 3.96LEE72 pKa = 4.52KK73 pKa = 11.14ANGMEE78 pKa = 4.16EE79 pKa = 3.97DD80 pKa = 3.91WEE82 pKa = 4.19NSLMKK87 pKa = 9.95IIEE90 pKa = 4.46SSDD93 pKa = 3.44VTPKK97 pKa = 10.86LSWNDD102 pKa = 2.99EE103 pKa = 4.41FEE105 pKa = 4.07NCTYY109 pKa = 10.56KK110 pKa = 10.94GYY112 pKa = 10.65VVSSEE117 pKa = 4.44DD118 pKa = 3.37LCNDD122 pKa = 3.67SGNQEE127 pKa = 4.12KK128 pKa = 11.24NEE130 pKa = 3.89VPIKK134 pKa = 10.29QSSLEE139 pKa = 4.03DD140 pKa = 3.48VAQVLSLFQIRR151 pKa = 11.84SEE153 pKa = 3.68VDD155 pKa = 3.1YY156 pKa = 11.16KK157 pKa = 10.75IEE159 pKa = 4.4KK160 pKa = 9.85DD161 pKa = 3.24NKK163 pKa = 8.97NQVKK167 pKa = 10.11IIKK170 pKa = 9.86LSKK173 pKa = 9.17QDD175 pKa = 3.46KK176 pKa = 9.3SVKK179 pKa = 9.79SKK181 pKa = 11.15RR182 pKa = 11.84NDD184 pKa = 3.24VVNQDD189 pKa = 3.0SDD191 pKa = 3.17KK192 pKa = 11.34HH193 pKa = 5.38MKK195 pKa = 9.95YY196 pKa = 10.52DD197 pKa = 3.82SNFDD201 pKa = 3.35AVMDD205 pKa = 3.71QFQKK209 pKa = 11.06GIRR212 pKa = 11.84IKK214 pKa = 10.67KK215 pKa = 9.58RR216 pKa = 11.84FGKK219 pKa = 10.67GYY221 pKa = 10.97VKK223 pKa = 10.44INADD227 pKa = 3.65NMPGTYY233 pKa = 9.53HH234 pKa = 7.41DD235 pKa = 4.62LSNVISTVKK244 pKa = 10.59GEE246 pKa = 4.12KK247 pKa = 9.13TVEE250 pKa = 3.53EE251 pKa = 4.31MIRR254 pKa = 11.84YY255 pKa = 8.28LFKK258 pKa = 10.3KK259 pKa = 9.76SKK261 pKa = 9.91RR262 pKa = 11.84FKK264 pKa = 10.9SINKK268 pKa = 7.94TLNIDD273 pKa = 4.13EE274 pKa = 5.35MILCC278 pKa = 4.91

Molecular weight: 32.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q65477|VPA3_BEFVB Protein alpha-3 OS=Bovine ephemeral fever virus (strain BB7721) OX=928297 GN=alpha PE=4 SV=1
MM1 pKa = 7.65EE2 pKa = 6.12KK3 pKa = 10.88GLLSNFWNDD12 pKa = 3.05FKK14 pKa = 11.03RR15 pKa = 11.84WSEE18 pKa = 3.84DD19 pKa = 2.82RR20 pKa = 11.84KK21 pKa = 10.48VEE23 pKa = 4.58IVIWWSNLEE32 pKa = 4.15SKK34 pKa = 10.51VRR36 pKa = 11.84LGFWIILIILLGILAIRR53 pKa = 11.84IAIKK57 pKa = 10.35VYY59 pKa = 10.2QCVKK63 pKa = 9.48FTNQGVKK70 pKa = 9.99KK71 pKa = 9.53IKK73 pKa = 10.34RR74 pKa = 11.84IIKK77 pKa = 9.67RR78 pKa = 11.84KK79 pKa = 9.78RR80 pKa = 11.84SIKK83 pKa = 10.18KK84 pKa = 9.16YY85 pKa = 10.5RR86 pKa = 11.84KK87 pKa = 6.76TT88 pKa = 3.33

Molecular weight: 10.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

4809

48

2144

400.8

46.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.786 ± 0.407

1.788 ± 0.229

5.614 ± 0.3

7.819 ± 0.328

4.096 ± 0.267

5.469 ± 0.298

2.371 ± 0.238

8.526 ± 0.435

8.775 ± 0.443

9.753 ± 0.716

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.682 ± 0.389

6.904 ± 0.224

3.244 ± 0.259

3.036 ± 0.168

5.136 ± 0.293

7.112 ± 0.52

4.284 ± 0.294

4.72 ± 0.392

1.83 ± 0.189

4.055 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski