Pseudoflavonifractor sp. An184

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Pseudoflavonifractor; unclassified Pseudoflavonifractor

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3242 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4LKU0|A0A1Y4LKU0_9FIRM Uncharacterized protein OS=Pseudoflavonifractor sp. An184 OX=1965576 GN=B5F19_08760 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.35RR3 pKa = 11.84NAALAAIALLASFACSACAPASPSGQTPEE32 pKa = 4.03FFHH35 pKa = 7.73DD36 pKa = 3.03IGKK39 pKa = 7.99TLSEE43 pKa = 4.88LKK45 pKa = 9.65TEE47 pKa = 4.15HH48 pKa = 7.23PEE50 pKa = 4.04GEE52 pKa = 4.5FIVNLDD58 pKa = 3.77GFPDD62 pKa = 3.4HH63 pKa = 7.21AAICFGDD70 pKa = 3.52PGAEE74 pKa = 3.64YY75 pKa = 9.78FYY77 pKa = 11.09YY78 pKa = 10.76FFGTQSGDD86 pKa = 2.96AEE88 pKa = 4.25KK89 pKa = 11.24AMDD92 pKa = 4.21EE93 pKa = 5.28LGDD96 pKa = 3.72QLKK99 pKa = 10.61CAGAITTANVLFPDD113 pKa = 3.99MEE115 pKa = 4.81EE116 pKa = 4.47DD117 pKa = 3.14MSFADD122 pKa = 4.81FFSLIGVEE130 pKa = 4.21EE131 pKa = 4.19YY132 pKa = 10.46EE133 pKa = 4.22YY134 pKa = 11.35LGADD138 pKa = 3.5TVAEE142 pKa = 4.16GWIRR146 pKa = 11.84FLYY149 pKa = 10.43RR150 pKa = 11.84DD151 pKa = 4.0MEE153 pKa = 4.65VMVSPTGDD161 pKa = 3.69GPDD164 pKa = 3.98GDD166 pKa = 3.57WDD168 pKa = 3.86VTGEE172 pKa = 4.1EE173 pKa = 4.07LVKK176 pKa = 11.05YY177 pKa = 9.15NAAVSVMDD185 pKa = 5.16PEE187 pKa = 5.0IEE189 pKa = 4.34SANSDD194 pKa = 3.55LADD197 pKa = 2.69AVMFDD202 pKa = 3.53EE203 pKa = 4.87TAAA206 pKa = 3.68

Molecular weight: 22.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4LGL9|A0A1Y4LGL9_9FIRM Uncharacterized protein (Fragment) OS=Pseudoflavonifractor sp. An184 OX=1965576 GN=B5F19_07445 PE=4 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.8QPKK8 pKa = 9.6KK9 pKa = 7.95RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.69EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 10.07RR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84VRR41 pKa = 11.84LTHH44 pKa = 6.42

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3242

0

3242

982754

35

2600

303.1

33.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.421 ± 0.054

1.519 ± 0.016

5.577 ± 0.036

7.078 ± 0.051

3.741 ± 0.031

7.906 ± 0.044

1.819 ± 0.018

5.265 ± 0.043

4.448 ± 0.045

10.357 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.659 ± 0.021

3.152 ± 0.03

4.375 ± 0.029

3.956 ± 0.027

5.964 ± 0.05

5.592 ± 0.039

5.439 ± 0.044

7.032 ± 0.037

1.181 ± 0.017

3.518 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski