Wenzhou sobemo-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEG7|A0A1L3KEG7_9VIRU RNA-directed RNA polymerase OS=Wenzhou sobemo-like virus 4 OX=1923660 PE=4 SV=1
MM1 pKa = 7.63PANGLHH7 pKa = 5.95VPDD10 pKa = 4.42TFGEE14 pKa = 4.22LPYY17 pKa = 10.71GIPQVDD23 pKa = 3.19ACSAKK28 pKa = 9.87RR29 pKa = 11.84SFRR32 pKa = 11.84AHH34 pKa = 5.89CQKK37 pKa = 10.52QKK39 pKa = 9.2QTDD42 pKa = 3.69APPTAEE48 pKa = 3.7EE49 pKa = 3.98RR50 pKa = 11.84TRR52 pKa = 11.84AVQTALRR59 pKa = 11.84SYY61 pKa = 9.84SAWRR65 pKa = 11.84FDD67 pKa = 3.73LTSAADD73 pKa = 3.56PNAWFDD79 pKa = 4.27SEE81 pKa = 4.42FNKK84 pKa = 10.48ALKK87 pKa = 10.01EE88 pKa = 3.51IDD90 pKa = 3.6MNSTPGYY97 pKa = 11.06CMLTNLGSNNAQIFGWDD114 pKa = 3.71GLTFDD119 pKa = 5.12PDD121 pKa = 3.13RR122 pKa = 11.84VSLVRR127 pKa = 11.84QHH129 pKa = 5.49VKK131 pKa = 10.53LRR133 pKa = 11.84FDD135 pKa = 3.34EE136 pKa = 4.63LLYY139 pKa = 11.4GNAVCDD145 pKa = 4.57DD146 pKa = 3.2IKK148 pKa = 11.45VFVKK152 pKa = 10.13QEE154 pKa = 3.55PHH156 pKa = 6.28KK157 pKa = 10.69LKK159 pKa = 10.53KK160 pKa = 9.98IQDD163 pKa = 3.36GTYY166 pKa = 9.79RR167 pKa = 11.84LISAVSLIDD176 pKa = 3.52TLIDD180 pKa = 4.32RR181 pKa = 11.84ILFAWIARR189 pKa = 11.84AQLSTVGEE197 pKa = 4.63TPCLIGWSPVRR208 pKa = 11.84GGWRR212 pKa = 11.84LITNKK217 pKa = 9.65FANEE221 pKa = 3.78PVNCLDD227 pKa = 3.68RR228 pKa = 11.84SAWDD232 pKa = 3.36WTVQGYY238 pKa = 8.9LVDD241 pKa = 3.93LWILFLEE248 pKa = 4.37NLPVNPPEE256 pKa = 3.71WWLKK260 pKa = 8.27MMKK263 pKa = 10.07LRR265 pKa = 11.84FKK267 pKa = 11.1LLFEE271 pKa = 4.65DD272 pKa = 3.89AVFRR276 pKa = 11.84FEE278 pKa = 5.04DD279 pKa = 3.83GTRR282 pKa = 11.84VRR284 pKa = 11.84QGTKK288 pKa = 9.93GVMKK292 pKa = 10.06SGCYY296 pKa = 8.47LTILLNSLSQSLLHH310 pKa = 6.08YY311 pKa = 9.88IANKK315 pKa = 9.43RR316 pKa = 11.84CGRR319 pKa = 11.84PMALKK324 pKa = 10.34QPFSIGDD331 pKa = 3.59DD332 pKa = 3.71TVQEE336 pKa = 4.1AMSWLPEE343 pKa = 3.85YY344 pKa = 10.66VRR346 pKa = 11.84QLEE349 pKa = 4.15ILGITVKK356 pKa = 10.34GAKK359 pKa = 7.74VQHH362 pKa = 5.26WVEE365 pKa = 3.88FAGFCFDD372 pKa = 6.05GKK374 pKa = 8.79TCYY377 pKa = 8.86PAYY380 pKa = 8.17WQKK383 pKa = 11.1HH384 pKa = 4.58LFNLKK389 pKa = 8.15HH390 pKa = 4.81TQRR393 pKa = 11.84LGEE396 pKa = 4.09TLMSYY401 pKa = 10.41QYY403 pKa = 11.1LYY405 pKa = 11.1VNEE408 pKa = 3.83PVMYY412 pKa = 10.47EE413 pKa = 3.89FVCRR417 pKa = 11.84VARR420 pKa = 11.84EE421 pKa = 3.85LGPRR425 pKa = 11.84NVLPRR430 pKa = 11.84IEE432 pKa = 5.34ALDD435 pKa = 3.55IMNQPRR441 pKa = 3.26

Molecular weight: 50.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEG7|A0A1L3KEG7_9VIRU RNA-directed RNA polymerase OS=Wenzhou sobemo-like virus 4 OX=1923660 PE=4 SV=1
MM1 pKa = 7.53TIYY4 pKa = 10.93ANVMEE9 pKa = 4.72ILYY12 pKa = 10.74SSVIYY17 pKa = 9.35VQIVAMVILAITALNVVNPLNKK39 pKa = 9.92TMILVSFGTIVAVLLRR55 pKa = 11.84SILYY59 pKa = 9.15WIEE62 pKa = 3.65TMVIMTYY69 pKa = 8.93GTAKK73 pKa = 10.28KK74 pKa = 9.76IWVEE78 pKa = 4.18LIFQRR83 pKa = 11.84FVNNLGTDD91 pKa = 3.54PNKK94 pKa = 10.11EE95 pKa = 3.99LKK97 pKa = 10.71LLIVMALIIIFMYY110 pKa = 9.18IWKK113 pKa = 9.09VCKK116 pKa = 10.04YY117 pKa = 8.93LQSTKK122 pKa = 9.98VVYY125 pKa = 9.53VVPSKK130 pKa = 10.87VKK132 pKa = 10.62KK133 pKa = 10.61GDD135 pKa = 3.1ACYY138 pKa = 10.46VEE140 pKa = 4.96EE141 pKa = 4.05RR142 pKa = 11.84AMPNSIYY149 pKa = 10.65EE150 pKa = 4.38HH151 pKa = 6.55IKK153 pKa = 10.39VLPPFQAMVMASLDD167 pKa = 3.63GYY169 pKa = 9.56KK170 pKa = 10.09YY171 pKa = 10.61HH172 pKa = 8.33VMGQCFWVDD181 pKa = 3.29EE182 pKa = 4.46GLVTAAHH189 pKa = 5.9VIEE192 pKa = 5.14GFEE195 pKa = 4.13HH196 pKa = 6.27LCIYY200 pKa = 10.5RR201 pKa = 11.84DD202 pKa = 3.8LEE204 pKa = 3.8HH205 pKa = 7.18SINIKK210 pKa = 9.18ATEE213 pKa = 4.25FEE215 pKa = 4.9CGNGDD220 pKa = 3.56YY221 pKa = 10.67AVCRR225 pKa = 11.84EE226 pKa = 4.22PTKK229 pKa = 9.83ITQKK233 pKa = 10.69VGLSKK238 pKa = 11.14AKK240 pKa = 10.02LSPVAVQKK248 pKa = 11.21NGGVTVNVSAMDD260 pKa = 3.31KK261 pKa = 9.59RR262 pKa = 11.84TAGFLDD268 pKa = 3.73EE269 pKa = 4.27HH270 pKa = 5.68TQFGFVTYY278 pKa = 9.83TGSTTKK284 pKa = 10.42GFSGAPYY291 pKa = 9.59CFGRR295 pKa = 11.84TVYY298 pKa = 10.77GMHH301 pKa = 7.16LGSDD305 pKa = 4.44SKK307 pKa = 12.08NMGYY311 pKa = 10.69DD312 pKa = 3.41GAFLKK317 pKa = 11.14AEE319 pKa = 4.72LKK321 pKa = 9.94PSRR324 pKa = 11.84LIKK327 pKa = 10.31QKK329 pKa = 11.0LGIKK333 pKa = 9.41TEE335 pKa = 5.2DD336 pKa = 3.34SAEE339 pKa = 3.98WLIGQVDD346 pKa = 4.48KK347 pKa = 11.38YY348 pKa = 11.69AEE350 pKa = 4.25LDD352 pKa = 3.78YY353 pKa = 11.29VRR355 pKa = 11.84SPGNPDD361 pKa = 2.59EE362 pKa = 4.0WRR364 pKa = 11.84VRR366 pKa = 11.84VGGRR370 pKa = 11.84YY371 pKa = 9.23HH372 pKa = 7.13LVDD375 pKa = 4.62DD376 pKa = 4.61EE377 pKa = 5.23VMATLLQARR386 pKa = 11.84KK387 pKa = 8.16GTEE390 pKa = 3.6VRR392 pKa = 11.84IGKK395 pKa = 9.75LKK397 pKa = 10.66DD398 pKa = 3.54FQRR401 pKa = 11.84EE402 pKa = 3.97ALFSRR407 pKa = 11.84GEE409 pKa = 3.85ISKK412 pKa = 10.64KK413 pKa = 10.47SSNHH417 pKa = 5.35PKK419 pKa = 9.9LAKK422 pKa = 10.63EE423 pKa = 4.0EE424 pKa = 4.26DD425 pKa = 3.69PEE427 pKa = 4.1KK428 pKa = 11.47AMVKK432 pKa = 10.42GLLAEE437 pKa = 5.05FGRR440 pKa = 11.84TSIVTEE446 pKa = 3.86EE447 pKa = 4.0PMVRR451 pKa = 11.84EE452 pKa = 4.31SGPSVDD458 pKa = 4.89GLPLAPRR465 pKa = 11.84DD466 pKa = 3.49AMSFNDD472 pKa = 3.53SGNLLRR478 pKa = 11.84APTVDD483 pKa = 3.29VGALGVEE490 pKa = 4.1NVRR493 pKa = 11.84RR494 pKa = 11.84EE495 pKa = 4.12FVRR498 pKa = 11.84NPSLQTCQQTVCTCQTPSVSYY519 pKa = 10.72HH520 pKa = 4.6MEE522 pKa = 3.86SRR524 pKa = 11.84KK525 pKa = 10.5SMPAQQSEE533 pKa = 3.91ASARR537 pKa = 11.84TVKK540 pKa = 10.66NKK542 pKa = 8.62NRR544 pKa = 11.84RR545 pKa = 11.84MLRR548 pKa = 11.84QQQKK552 pKa = 10.54KK553 pKa = 8.68EE554 pKa = 3.91LEE556 pKa = 4.32LYY558 pKa = 10.07KK559 pKa = 10.2QLCGPIQHH567 pKa = 6.79GASTLHH573 pKa = 5.78PQQTQMHH580 pKa = 7.16GSTQSLTKK588 pKa = 10.35HH589 pKa = 5.71

Molecular weight: 65.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1030

441

589

515.0

58.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.602 ± 0.122

2.136 ± 0.217

4.563 ± 0.67

5.728 ± 0.448

3.981 ± 0.611

6.505 ± 0.369

2.136 ± 0.195

5.049 ± 0.311

6.602 ± 0.703

9.515 ± 0.83

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.301 ± 0.489

3.883 ± 0.257

4.66 ± 0.474

4.66 ± 0.062

5.437 ± 0.553

5.728 ± 0.585

5.728 ± 0.311

8.155 ± 0.957

1.942 ± 0.747

3.689 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski