Peach associated luteovirus
Average proteome isoelectric point is 7.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z1G759|A0A1Z1G759_9LUTE Movement protein OS=Peach associated luteovirus OX=2006498 GN=ORF4 PE=4 SV=1
MM1 pKa = 7.47 IVNLTQGAHH10 pKa = 6.21 LALPGGSYY18 pKa = 10.76 SSWLLSQISDD28 pKa = 3.7 CGFTEE33 pKa = 4.8 ACCAITTCGAYY44 pKa = 8.89 TPHH47 pKa = 7.28 WLL49 pKa = 4.03
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.397
IPC2_protein 5.283
IPC_protein 4.914
Toseland 5.092
ProMoST 5.207
Dawson 5.08
Bjellqvist 5.143
Wikipedia 5.067
Rodwell 5.029
Grimsley 5.118
Solomon 5.067
Lehninger 5.041
Nozaki 5.308
DTASelect 5.448
Thurlkill 5.334
EMBOSS 5.296
Sillero 5.359
Patrickios 0.006
IPC_peptide 5.08
IPC2_peptide 5.372
IPC2.peptide.svr19 5.398
Protein with the highest isoelectric point:
>tr|A0A1Z1G758|A0A1Z1G758_9LUTE Isoform of A0A1Z1G754 Coat protein OS=Peach associated luteovirus OX=2006498 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.05 PTKK4 pKa = 10.25 KK5 pKa = 10.68 GKK7 pKa = 10.82 GKK9 pKa = 10.43 GKK11 pKa = 10.11 KK12 pKa = 9.19 GKK14 pKa = 10.46 KK15 pKa = 8.96 NGKK18 pKa = 9.04 GGNSGANAKK27 pKa = 10.14 SVVVNVQSGGRR38 pKa = 11.84 GRR40 pKa = 11.84 TGRR43 pKa = 11.84 SASGGNRR50 pKa = 11.84 VAGSGPGDD58 pKa = 3.37 HH59 pKa = 7.12 SNRR62 pKa = 11.84 FSFTVDD68 pKa = 3.51 DD69 pKa = 4.9 LNSASSGILKK79 pKa = 9.99 FGPNLSQYY87 pKa = 10.07 TNFSNGILKK96 pKa = 10.33 SFHH99 pKa = 6.61 EE100 pKa = 4.24 YY101 pKa = 10.62 KK102 pKa = 9.4 ITNLTVKK109 pKa = 9.48 YY110 pKa = 10.31 VSYY113 pKa = 11.06 ASSTTSGAFAIEE125 pKa = 4.05 IDD127 pKa = 3.85 TSRR130 pKa = 11.84 KK131 pKa = 7.13 QTDD134 pKa = 3.5 LKK136 pKa = 11.16 SRR138 pKa = 11.84 IISFPVSKK146 pKa = 10.48 GFSRR150 pKa = 11.84 GFQARR155 pKa = 11.84 VLRR158 pKa = 11.84 GLLWHH163 pKa = 6.63 PTSEE167 pKa = 4.16 DD168 pKa = 3.67 QFWLVYY174 pKa = 10.01 KK175 pKa = 10.98 GNGKK179 pKa = 8.5 STDD182 pKa = 3.0 IAGQFVISFNVNFQGPCC199 pKa = 3.06
Molecular weight: 21.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.221
IPC2_protein 9.75
IPC_protein 9.882
Toseland 10.672
ProMoST 10.189
Dawson 10.774
Bjellqvist 10.365
Wikipedia 10.891
Rodwell 11.359
Grimsley 10.804
Solomon 10.804
Lehninger 10.789
Nozaki 10.643
DTASelect 10.365
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.672
Patrickios 11.082
IPC_peptide 10.818
IPC2_peptide 8.902
IPC2.peptide.svr19 8.688
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
2
8
2454
49
890
306.8
34.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.031 ± 0.457
1.711 ± 0.393
5.786 ± 0.582
5.216 ± 0.692
4.686 ± 0.486
6.724 ± 1.351
1.997 ± 0.295
4.849 ± 0.282
6.724 ± 0.609
7.865 ± 0.889
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.812 ± 0.574
4.401 ± 0.645
5.338 ± 0.702
4.645 ± 0.243
5.542 ± 0.365
8.517 ± 1.324
4.808 ± 0.8
8.191 ± 0.819
1.385 ± 0.187
2.73 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here