Podoviridae sp. ctbd591

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345BNP6|A0A345BNP6_9CAUD DNA-directed DNA polymerase OS=Podoviridae sp. ctbd591 OX=2675445 PE=4 SV=1
MM1 pKa = 7.75SYY3 pKa = 11.28LDD5 pKa = 4.77DD6 pKa = 4.78GCHH9 pKa = 4.3NTYY12 pKa = 10.61FNGVPDD18 pKa = 4.26GCNKK22 pKa = 9.54KK23 pKa = 9.52HH24 pKa = 6.46CNNHH28 pKa = 4.37GCYY31 pKa = 9.93DD32 pKa = 4.01RR33 pKa = 11.84PPNCEE38 pKa = 3.79PNKK41 pKa = 10.36LDD43 pKa = 3.73SCEE46 pKa = 4.31TGCLPPPIKK55 pKa = 10.06PMCIIPGMNSQQQMSVIVRR74 pKa = 11.84KK75 pKa = 8.15TNEE78 pKa = 4.24CIEE81 pKa = 4.16RR82 pKa = 11.84WNTIQSNCYY91 pKa = 8.75EE92 pKa = 4.34ALNKK96 pKa = 10.35CVGAAVSNGVYY107 pKa = 10.17YY108 pKa = 10.69DD109 pKa = 3.79CEE111 pKa = 4.1EE112 pKa = 3.88VRR114 pKa = 11.84LQNGYY119 pKa = 9.6SEE121 pKa = 5.38NDD123 pKa = 3.08NCPYY127 pKa = 10.24KK128 pKa = 10.86YY129 pKa = 10.93VVIKK133 pKa = 10.11ATDD136 pKa = 3.3KK137 pKa = 10.62KK138 pKa = 10.76GCPIKK143 pKa = 11.04VKK145 pKa = 10.89LKK147 pKa = 8.88TAYY150 pKa = 10.24GNSGNNGARR159 pKa = 11.84QSMQDD164 pKa = 2.64ASFIYY169 pKa = 10.18SANTIISAINPVSGRR184 pKa = 11.84WSGPALIDD192 pKa = 3.87GCPMEE197 pKa = 4.58YY198 pKa = 9.61IKK200 pKa = 10.94PEE202 pKa = 4.23TIVDD206 pKa = 3.57GDD208 pKa = 3.68VLYY211 pKa = 11.09GFNKK215 pKa = 9.07GGAMLCFGPNTEE227 pKa = 4.73LEE229 pKa = 4.25TLKK232 pKa = 9.89MCGMVDD238 pKa = 3.81VIGMGIQILKK248 pKa = 10.23DD249 pKa = 3.67GNVTAAAEE257 pKa = 4.18SLTTGASIQAIGYY270 pKa = 9.2KK271 pKa = 9.81SSCGEE276 pKa = 3.96KK277 pKa = 10.68VFFSCGKK284 pKa = 9.02EE285 pKa = 3.9NSPGMQGINVANVLKK300 pKa = 11.27NMGCTTAVITCLQTMYY316 pKa = 10.89PDD318 pKa = 3.27ITGTTYY324 pKa = 10.68RR325 pKa = 11.84GIEE328 pKa = 4.12NAVTPASDD336 pKa = 5.62AIQQKK341 pKa = 8.88TGLTGGVMFLGRR353 pKa = 11.84LLNPPLEE360 pKa = 4.09WQIPYY365 pKa = 9.96NQAFWVVSKK374 pKa = 10.83KK375 pKa = 10.19PSNGFRR381 pKa = 11.84NDD383 pKa = 2.88FTTEE387 pKa = 4.01VADD390 pKa = 3.88LTQSLGGSVNNMASFANVIDD410 pKa = 3.75NMQIDD415 pKa = 4.39VLEE418 pKa = 4.3VTQKK422 pKa = 10.15IEE424 pKa = 4.35KK425 pKa = 10.02IEE427 pKa = 4.21SDD429 pKa = 3.4VSNISEE435 pKa = 5.13SITQLEE441 pKa = 4.34TRR443 pKa = 11.84VNAIEE448 pKa = 4.24PKK450 pKa = 9.24LTNLEE455 pKa = 4.2SDD457 pKa = 3.67VEE459 pKa = 4.34TLKK462 pKa = 11.0NCCNTVQEE470 pKa = 4.4YY471 pKa = 8.72MVTEE475 pKa = 4.09TEE477 pKa = 3.97EE478 pKa = 4.43RR479 pKa = 11.84KK480 pKa = 10.15QSDD483 pKa = 3.42TALGDD488 pKa = 4.04RR489 pKa = 11.84ITALQIAIQEE499 pKa = 4.3EE500 pKa = 4.56ATTRR504 pKa = 11.84NDD506 pKa = 3.27ADD508 pKa = 3.32NRR510 pKa = 11.84LIEE513 pKa = 4.94AIQNEE518 pKa = 4.14ASARR522 pKa = 11.84ATNDD526 pKa = 2.79AKK528 pKa = 11.11LDD530 pKa = 3.67TKK532 pKa = 10.6IDD534 pKa = 3.77SEE536 pKa = 4.61VAKK539 pKa = 10.62LEE541 pKa = 4.24SAIQDD546 pKa = 3.57IEE548 pKa = 4.38VAAYY552 pKa = 7.31TAGEE556 pKa = 4.2GIIINNRR563 pKa = 11.84VISVNKK569 pKa = 10.2DD570 pKa = 3.25DD571 pKa = 5.79FPDD574 pKa = 3.6NEE576 pKa = 4.89KK577 pKa = 10.47ISEE580 pKa = 4.26LEE582 pKa = 4.02NKK584 pKa = 9.39VNALPNYY591 pKa = 9.3VAGEE595 pKa = 4.41HH596 pKa = 6.52IVFTQSGGNTSISADD611 pKa = 3.43LTDD614 pKa = 4.15LSEE617 pKa = 4.63EE618 pKa = 4.06IEE620 pKa = 4.15NTKK623 pKa = 10.27TIVQEE628 pKa = 3.99NTSKK632 pKa = 10.55IEE634 pKa = 3.88KK635 pKa = 10.48AEE637 pKa = 3.87GDD639 pKa = 3.61IEE641 pKa = 4.52NNASSITTIEE651 pKa = 4.2NEE653 pKa = 3.9VNTIKK658 pKa = 11.06GDD660 pKa = 3.48ISQLQSDD667 pKa = 4.2VKK669 pKa = 10.24EE670 pKa = 4.29ANISAGNGIEE680 pKa = 4.19INTVDD685 pKa = 3.91DD686 pKa = 4.02SKK688 pKa = 11.24EE689 pKa = 3.68ISVKK693 pKa = 9.92IDD695 pKa = 3.2STLSFQPDD703 pKa = 3.42GALHH707 pKa = 6.42VDD709 pKa = 4.3LPAPEE714 pKa = 4.37TGEE717 pKa = 4.2TTRR720 pKa = 11.84AGYY723 pKa = 10.33GIIISKK729 pKa = 10.64NEE731 pKa = 4.56DD732 pKa = 2.93IATIAVNQQLVASTADD748 pKa = 3.1IQNIKK753 pKa = 10.54SGTTPLPYY761 pKa = 10.52VKK763 pKa = 10.55VSGDD767 pKa = 2.89IMTGEE772 pKa = 4.88LIGTTFKK779 pKa = 11.43SNVEE783 pKa = 3.71MPIRR787 pKa = 11.84DD788 pKa = 3.58NNEE791 pKa = 3.25NHH793 pKa = 6.31SAFGVDD799 pKa = 4.3VEE801 pKa = 4.88DD802 pKa = 4.43NKK804 pKa = 11.09LKK806 pKa = 10.9YY807 pKa = 10.54GGVGEE812 pKa = 3.94NTVFNNRR819 pKa = 11.84VKK821 pKa = 9.55ITNVDD826 pKa = 3.79TPSGDD831 pKa = 3.32TDD833 pKa = 3.83ATPKK837 pKa = 10.78DD838 pKa = 3.83YY839 pKa = 11.63VDD841 pKa = 4.02NKK843 pKa = 10.6DD844 pKa = 3.59DD845 pKa = 4.45AVISMTNTAIKK856 pKa = 10.21SAEE859 pKa = 3.78DD860 pKa = 3.9RR861 pKa = 11.84IEE863 pKa = 4.31GEE865 pKa = 3.62IAANTTEE872 pKa = 4.07INEE875 pKa = 4.53KK876 pKa = 10.26IEE878 pKa = 4.15STKK881 pKa = 11.07NEE883 pKa = 4.06LQNNINVIDD892 pKa = 3.92EE893 pKa = 4.63AQTEE897 pKa = 4.2LQEE900 pKa = 4.14QFSKK904 pKa = 11.47LEE906 pKa = 4.69DD907 pKa = 3.98GSVSLPYY914 pKa = 10.3VKK916 pKa = 10.53KK917 pKa = 10.87SGDD920 pKa = 3.67SMSGPLSLVDD930 pKa = 3.17STGAVLGQLSLTEE943 pKa = 4.22NGLMILGGPAATGAHH958 pKa = 4.89LTITNEE964 pKa = 4.15SVSIDD969 pKa = 3.5KK970 pKa = 8.0PTHH973 pKa = 5.51VNGTVSANNATKK985 pKa = 10.67DD986 pKa = 3.65NEE988 pKa = 4.44LVTLEE993 pKa = 4.08QLNAKK998 pKa = 9.67PSGISQTDD1006 pKa = 2.61ADD1008 pKa = 3.82ARR1010 pKa = 11.84YY1011 pKa = 8.4IASSNGVSHH1020 pKa = 6.41TPVYY1024 pKa = 10.47FDD1026 pKa = 5.52AEE1028 pKa = 4.15EE1029 pKa = 4.49DD1030 pKa = 3.75LNGWVHH1036 pKa = 7.5IYY1038 pKa = 10.81RR1039 pKa = 11.84LTKK1042 pKa = 10.87GNGLQSAINHH1052 pKa = 6.24NGIQYY1057 pKa = 9.94NSKK1060 pKa = 10.82DD1061 pKa = 3.08ITTTIDD1067 pKa = 3.37NPIPLQIGNPTLPSHH1082 pKa = 7.08AANKK1086 pKa = 10.31SYY1088 pKa = 11.32VDD1090 pKa = 3.81AVASSVGDD1098 pKa = 3.64GKK1100 pKa = 10.8FLPLTGGTMSGPIYY1114 pKa = 9.55ITEE1117 pKa = 4.2HH1118 pKa = 6.39NAFQSPGRR1126 pKa = 11.84QVPTFFSIDD1135 pKa = 3.47ARR1137 pKa = 11.84SGLYY1141 pKa = 10.45FSDD1144 pKa = 5.04DD1145 pKa = 3.4NNLEE1149 pKa = 4.13QNPVRR1154 pKa = 11.84VSGVGDD1160 pKa = 3.41PTIDD1164 pKa = 2.93IDD1166 pKa = 4.14AANKK1170 pKa = 10.19RR1171 pKa = 11.84YY1172 pKa = 10.02VDD1174 pKa = 3.8SKK1176 pKa = 10.12TDD1178 pKa = 3.33GLGYY1182 pKa = 9.78PVVKK1186 pKa = 10.4SIQEE1190 pKa = 4.03LVSAAVAGATKK1201 pKa = 9.6TISGGMVLTWGRR1213 pKa = 11.84LLYY1216 pKa = 10.29ISITFRR1222 pKa = 11.84VDD1224 pKa = 2.88EE1225 pKa = 4.3TLSNGTRR1232 pKa = 11.84LPFYY1236 pKa = 10.65VQLPSQGFYY1245 pKa = 10.37QGNPLVSATPGVGVGTTPYY1264 pKa = 10.95CNITVGTPLYY1274 pKa = 10.16SGSNMFVQGCIFGTDD1289 pKa = 2.93TYY1291 pKa = 11.07ALQNITVTMLGCAA1304 pKa = 3.83

Molecular weight: 140.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345BNN9|A0A345BNN9_9CAUD Uncharacterized protein OS=Podoviridae sp. ctbd591 OX=2675445 PE=4 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.56RR4 pKa = 11.84KK5 pKa = 9.2NSKK8 pKa = 8.72NKK10 pKa = 10.45GYY12 pKa = 8.52ITLPGGRR19 pKa = 11.84VRR21 pKa = 11.84RR22 pKa = 11.84STIDD26 pKa = 3.13NYY28 pKa = 11.0RR29 pKa = 11.84KK30 pKa = 9.59AVKK33 pKa = 10.11NYY35 pKa = 9.21NEE37 pKa = 4.54RR38 pKa = 11.84IRR40 pKa = 11.84YY41 pKa = 8.1AKK43 pKa = 10.26RR44 pKa = 11.84KK45 pKa = 8.65FGNYY49 pKa = 9.73DD50 pKa = 3.71YY51 pKa = 11.1LPKK54 pKa = 10.11PIKK57 pKa = 10.51ASEE60 pKa = 4.11TLKK63 pKa = 11.09GFTDD67 pKa = 4.45GKK69 pKa = 9.87SIRR72 pKa = 11.84DD73 pKa = 4.46FISDD77 pKa = 3.26INKK80 pKa = 9.56INISNLTPVEE90 pKa = 4.26TKK92 pKa = 10.69AGVITKK98 pKa = 10.7GEE100 pKa = 3.97LKK102 pKa = 10.05MIKK105 pKa = 8.88TRR107 pKa = 11.84IKK109 pKa = 10.49RR110 pKa = 11.84EE111 pKa = 3.79NKK113 pKa = 9.39RR114 pKa = 11.84RR115 pKa = 11.84AQVKK119 pKa = 10.32KK120 pKa = 10.8LVDD123 pKa = 3.39KK124 pKa = 11.14QEE126 pKa = 3.88NEE128 pKa = 3.26IGYY131 pKa = 9.93FKK133 pKa = 10.58TDD135 pKa = 2.79NDD137 pKa = 4.79KK138 pKa = 11.12ILMPIDD144 pKa = 3.09IEE146 pKa = 4.35KK147 pKa = 10.82YY148 pKa = 10.17NNLEE152 pKa = 3.86SLRR155 pKa = 11.84RR156 pKa = 11.84LSDD159 pKa = 4.25RR160 pKa = 11.84ISVDD164 pKa = 3.14YY165 pKa = 10.74RR166 pKa = 11.84NKK168 pKa = 10.64LAFDD172 pKa = 3.13WKK174 pKa = 10.64DD175 pKa = 3.21RR176 pKa = 11.84YY177 pKa = 10.2VNQVTFVINQGLISGLSQDD196 pKa = 4.21DD197 pKa = 4.26EE198 pKa = 5.21IIVRR202 pKa = 11.84LEE204 pKa = 4.02TIKK207 pKa = 10.66RR208 pKa = 11.84WVLSLDD214 pKa = 3.07NTTDD218 pKa = 2.9ITRR221 pKa = 11.84AAYY224 pKa = 8.24AAPSMDD230 pKa = 2.61ITVIYY235 pKa = 9.95YY236 pKa = 9.64SEE238 pKa = 3.96ISGPIVSEE246 pKa = 4.72MYY248 pKa = 10.87NDD250 pKa = 3.28WNLFMDD256 pKa = 4.31SLL258 pKa = 4.26

Molecular weight: 30.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17

0

17

5232

81

1304

307.8

34.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.657 ± 0.705

1.567 ± 0.306

5.944 ± 0.206

6.67 ± 0.526

4.434 ± 0.662

6.307 ± 0.659

1.281 ± 0.218

7.932 ± 0.547

6.384 ± 0.699

6.881 ± 0.619

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.214

8.008 ± 0.651

3.345 ± 0.418

2.867 ± 0.481

4.014 ± 0.58

7.607 ± 0.407

7.148 ± 1.084

6.097 ± 0.466

1.013 ± 0.252

4.587 ± 0.526
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski