Phreatobacter sp. NMCR1094

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phreatobacteraceae; Phreatobacter; unclassified Phreatobacter

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4643 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D7QJS5|A0A4D7QJS5_9PROT ABC transporter permease OS=Phreatobacter sp. NMCR1094 OX=2570229 GN=E8L99_07135 PE=3 SV=1
MM1 pKa = 7.29SLSGALNSAVSALSSQSSALAMISDD26 pKa = 5.01NISNSSTYY34 pKa = 10.57GYY36 pKa = 8.0KK37 pKa = 8.19TTTASFEE44 pKa = 4.42SMLTGSSSSAYY55 pKa = 9.8SSGGVSVSSVSNISTAGLLVASTTSSNMAISGSGFFVVTDD95 pKa = 4.38GANSNQTFYY104 pKa = 11.51SRR106 pKa = 11.84NGEE109 pKa = 4.04FTTDD113 pKa = 2.79SDD115 pKa = 4.54GYY117 pKa = 10.72LVNGDD122 pKa = 4.45YY123 pKa = 11.04YY124 pKa = 10.84LQGWKK129 pKa = 8.79TDD131 pKa = 3.33AEE133 pKa = 4.49GNIVGSSTQSSLTAIDD149 pKa = 3.9TNSVATIASATTKK162 pKa = 10.13MSLQANLPSNAATNDD177 pKa = 3.44TFTSEE182 pKa = 4.06VEE184 pKa = 4.49LYY186 pKa = 10.81DD187 pKa = 4.15SLGTAATSTVTWTKK201 pKa = 10.35TGDD204 pKa = 3.96NAWTASFSDD213 pKa = 3.64PTAADD218 pKa = 3.51GTTQLGTVSSSDD230 pKa = 2.91ITITFNTDD238 pKa = 2.01GTLASTSPSPATLSIGAWTTGAADD262 pKa = 3.51STITLDD268 pKa = 2.97MGTIGSSSGLTQLSSTADD286 pKa = 3.48TLAVSLTQDD295 pKa = 2.87QDD297 pKa = 3.55GVSYY301 pKa = 11.3GSLTGVEE308 pKa = 4.18IGDD311 pKa = 3.8NGTVYY316 pKa = 11.1ANYY319 pKa = 10.74DD320 pKa = 3.39NGEE323 pKa = 3.45QRR325 pKa = 11.84AIYY328 pKa = 9.02KK329 pKa = 9.85VAVATFNNADD339 pKa = 3.82GLTAMSGGVYY349 pKa = 9.95AASSEE354 pKa = 4.58SGGSTLHH361 pKa = 6.5IAGTGGAGDD370 pKa = 3.66VLGSKK375 pKa = 10.63LEE377 pKa = 4.17ASTTDD382 pKa = 3.27TNQEE386 pKa = 3.95FSNMMSAQQAYY397 pKa = 9.43SAAAQVMSTVNKK409 pKa = 10.0MYY411 pKa = 8.48DD412 pKa = 3.46TLISAVRR419 pKa = 3.41

Molecular weight: 42.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D7QFR8|A0A4D7QFR8_9PROT Glutathione S-transferase OS=Phreatobacter sp. NMCR1094 OX=2570229 GN=E8L99_13900 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.69GGQRR29 pKa = 11.84ILSARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4643

0

4643

1481849

27

3288

319.2

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.256 ± 0.051

0.784 ± 0.011

5.426 ± 0.027

5.126 ± 0.028

3.73 ± 0.024

8.834 ± 0.031

1.928 ± 0.019

5.345 ± 0.026

2.99 ± 0.03

9.958 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.576 ± 0.018

2.376 ± 0.019

5.47 ± 0.028

3.036 ± 0.027

7.31 ± 0.036

5.239 ± 0.029

5.554 ± 0.041

7.748 ± 0.03

1.299 ± 0.012

2.014 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski