Amino acid dipepetide frequency for Phreatobacter sp. NMCR1094

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.872AlaAla: 18.872 ± 0.169	1.109AlaCys: 1.109 ± 0.031	7.036AlaAsp: 7.036 ± 0.082	7.676AlaGlu: 7.676 ± 0.085	4.82AlaPhe: 4.82 ± 0.065	11.686AlaGly: 11.686 ± 0.104	2.274AlaHis: 2.274 ± 0.039	7.317AlaIle: 7.317 ± 0.078	4.216AlaLys: 4.216 ± 0.064	13.964AlaLeu: 13.964 ± 0.134	3.872AlaMet: 3.872 ± 0.052	2.946AlaAsn: 2.946 ± 0.041	6.063AlaPro: 6.063 ± 0.096	3.872AlaGln: 3.872 ± 0.062	9.546AlaArg: 9.546 ± 0.1	6.87AlaSer: 6.87 ± 0.074	6.741AlaThr: 6.741 ± 0.081	9.452AlaVal: 9.452 ± 0.089	1.595AlaTrp: 1.595 ± 0.031	2.637AlaTyr: 2.637 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	0.903CysAla: 0.903 ± 0.03	0.094CysCys: 0.094 ± 0.008	0.52CysAsp: 0.52 ± 0.02	0.37CysGlu: 0.37 ± 0.016	0.335CysPhe: 0.335 ± 0.015	0.839CysGly: 0.839 ± 0.026	0.215CysHis: 0.215 ± 0.011	0.352CysIle: 0.352 ± 0.014	0.14CysLys: 0.14 ± 0.01	0.767CysLeu: 0.767 ± 0.028	0.154CysMet: 0.154 ± 0.01	0.206CysAsn: 0.206 ± 0.012	0.446CysPro: 0.446 ± 0.019	0.223CysGln: 0.223 ± 0.013	0.626CysArg: 0.626 ± 0.023	0.416CysSer: 0.416 ± 0.018	0.396CysThr: 0.396 ± 0.019	0.577CysVal: 0.577 ± 0.02	0.099CysTrp: 0.099 ± 0.008	0.162CysTyr: 0.162 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	6.343AspAla: 6.343 ± 0.082	0.421AspCys: 0.421 ± 0.017	2.773AspAsp: 2.773 ± 0.054	3.091AspGlu: 3.091 ± 0.059	2.003AspPhe: 2.003 ± 0.041	4.963AspGly: 4.963 ± 0.063	1.236AspHis: 1.236 ± 0.035	3.076AspIle: 3.076 ± 0.046	1.543AspLys: 1.543 ± 0.032	6.05AspLeu: 6.05 ± 0.075	1.368AspMet: 1.368 ± 0.028	1.08AspAsn: 1.08 ± 0.025	3.569AspPro: 3.569 ± 0.051	1.812AspGln: 1.812 ± 0.036	4.582AspArg: 4.582 ± 0.082	1.814AspSer: 1.814 ± 0.037	2.371AspThr: 2.371 ± 0.044	4.085AspVal: 4.085 ± 0.052	0.821AspTrp: 0.821 ± 0.025	1.254AspTyr: 1.254 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	7.984GluAla: 7.984 ± 0.084	0.314GluCys: 0.314 ± 0.016	2.167GluAsp: 2.167 ± 0.042	2.452GluGlu: 2.452 ± 0.05	1.546GluPhe: 1.546 ± 0.032	3.907GluGly: 3.907 ± 0.05	0.947GluHis: 0.947 ± 0.027	3.172GluIle: 3.172 ± 0.046	1.782GluLys: 1.782 ± 0.038	4.553GluLeu: 4.553 ± 0.054	1.396GluMet: 1.396 ± 0.035	1.219GluAsn: 1.219 ± 0.029	2.836GluPro: 2.836 ± 0.051	1.77GluGln: 1.77 ± 0.041	4.808GluArg: 4.808 ± 0.067	1.906GluSer: 1.906 ± 0.035	3.403GluThr: 3.403 ± 0.051	3.774GluVal: 3.774 ± 0.055	0.578GluTrp: 0.578 ± 0.02	0.746GluTyr: 0.746 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	4.88PheAla: 4.88 ± 0.057	0.387PheCys: 0.387 ± 0.02	2.581PheAsp: 2.581 ± 0.042	1.964PheGlu: 1.964 ± 0.038	1.342PhePhe: 1.342 ± 0.03	3.971PheGly: 3.971 ± 0.058	0.738PheHis: 0.738 ± 0.023	1.687PheIle: 1.687 ± 0.041	0.976PheLys: 0.976 ± 0.028	3.296PheLeu: 3.296 ± 0.051	0.843PheMet: 0.843 ± 0.022	1.02PheAsn: 1.02 ± 0.028	1.677PhePro: 1.677 ± 0.036	1.107PheGln: 1.107 ± 0.028	2.387PheArg: 2.387 ± 0.04	2.067PheSer: 2.067 ± 0.038	1.975PheThr: 1.975 ± 0.038	3.032PheVal: 3.032 ± 0.052	0.524PheTrp: 0.524 ± 0.019	0.85PheTyr: 0.85 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	9.732GlyAla: 9.732 ± 0.085	0.812GlyCys: 0.812 ± 0.024	4.199GlyAsp: 4.199 ± 0.062	4.492GlyGlu: 4.492 ± 0.065	3.886GlyPhe: 3.886 ± 0.055	7.687GlyGly: 7.687 ± 0.09	1.961GlyHis: 1.961 ± 0.039	4.899GlyIle: 4.899 ± 0.063	2.889GlyLys: 2.889 ± 0.045	9.956GlyLeu: 9.956 ± 0.185	2.342GlyMet: 2.342 ± 0.042	1.921GlyAsn: 1.921 ± 0.034	4.127GlyPro: 4.127 ± 0.054	3.172GlyGln: 3.172 ± 0.055	6.814GlyArg: 6.814 ± 0.08	4.756GlySer: 4.756 ± 0.058	4.98GlyThr: 4.98 ± 0.066	6.095GlyVal: 6.095 ± 0.077	1.378GlyTrp: 1.378 ± 0.032	2.243GlyTyr: 2.243 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.354HisAla: 2.354 ± 0.039	0.215HisCys: 0.215 ± 0.012	1.115HisAsp: 1.115 ± 0.029	0.902HisGlu: 0.902 ± 0.026	0.774HisPhe: 0.774 ± 0.024	1.973HisGly: 1.973 ± 0.041	0.545HisHis: 0.545 ± 0.02	0.883HisIle: 0.883 ± 0.027	0.424HisLys: 0.424 ± 0.016	1.958HisLeu: 1.958 ± 0.037	0.472HisMet: 0.472 ± 0.018	0.402HisAsn: 0.402 ± 0.016	1.321HisPro: 1.321 ± 0.031	0.541HisGln: 0.541 ± 0.019	1.383HisArg: 1.383 ± 0.028	0.831HisSer: 0.831 ± 0.024	0.768HisThr: 0.768 ± 0.023	1.644HisVal: 1.644 ± 0.037	0.328HisTrp: 0.328 ± 0.014	0.445HisTyr: 0.445 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	8.155IleAla: 8.155 ± 0.075	0.496IleCys: 0.496 ± 0.02	3.611IleAsp: 3.611 ± 0.05	3.398IleGlu: 3.398 ± 0.049	1.618IlePhe: 1.618 ± 0.039	5.509IleGly: 5.509 ± 0.073	0.976IleHis: 0.976 ± 0.024	2.384IleIle: 2.384 ± 0.044	1.369IleLys: 1.369 ± 0.033	4.558IleLeu: 4.558 ± 0.068	1.074IleMet: 1.074 ± 0.029	1.326IleAsn: 1.326 ± 0.03	2.383IlePro: 2.383 ± 0.043	1.374IleGln: 1.374 ± 0.03	3.463IleArg: 3.463 ± 0.045	2.696IleSer: 2.696 ± 0.049	2.751IleThr: 2.751 ± 0.04	4.662IleVal: 4.662 ± 0.059	0.597IleTrp: 0.597 ± 0.024	1.045IleTyr: 1.045 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	4.591LysAla: 4.591 ± 0.059	0.122LysCys: 0.122 ± 0.01	1.52LysAsp: 1.52 ± 0.034	1.198LysGlu: 1.198 ± 0.034	0.819LysPhe: 0.819 ± 0.025	2.485LysGly: 2.485 ± 0.05	0.506LysHis: 0.506 ± 0.017	1.554LysIle: 1.554 ± 0.037	0.985LysLys: 0.985 ± 0.029	3.047LysLeu: 3.047 ± 0.051	0.618LysMet: 0.618 ± 0.022	0.661LysAsn: 0.661 ± 0.028	2.135LysPro: 2.135 ± 0.039	0.776LysGln: 0.776 ± 0.023	2.276LysArg: 2.276 ± 0.049	1.566LysSer: 1.566 ± 0.032	1.796LysThr: 1.796 ± 0.039	2.476LysVal: 2.476 ± 0.047	0.318LysTrp: 0.318 ± 0.016	0.454LysTyr: 0.454 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	15.11LeuAla: 15.11 ± 0.126	0.809LeuCys: 0.809 ± 0.025	5.701LeuAsp: 5.701 ± 0.069	4.384LeuGlu: 4.384 ± 0.058	3.669LeuPhe: 3.669 ± 0.056	8.823LeuGly: 8.823 ± 0.1	1.656LeuHis: 1.656 ± 0.033	5.21LeuIle: 5.21 ± 0.064	3.547LeuLys: 3.547 ± 0.049	8.314LeuLeu: 8.314 ± 0.104	2.597LeuMet: 2.597 ± 0.048	2.29LeuAsn: 2.29 ± 0.062	5.505LeuPro: 5.505 ± 0.067	2.415LeuGln: 2.415 ± 0.041	6.244LeuArg: 6.244 ± 0.074	6.122LeuSer: 6.122 ± 0.112	5.941LeuThr: 5.941 ± 0.159	8.298LeuVal: 8.298 ± 0.101	1.137LeuTrp: 1.137 ± 0.032	1.811LeuTyr: 1.811 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	3.525MetAla: 3.525 ± 0.049	0.15MetCys: 0.15 ± 0.009	1.133MetAsp: 1.133 ± 0.028	1.018MetGlu: 1.018 ± 0.025	0.781MetPhe: 0.781 ± 0.024	1.888MetGly: 1.888 ± 0.039	0.386MetHis: 0.386 ± 0.017	1.464MetIle: 1.464 ± 0.036	0.876MetLys: 0.876 ± 0.027	2.485MetLeu: 2.485 ± 0.041	0.694MetMet: 0.694 ± 0.023	0.696MetAsn: 0.696 ± 0.023	1.633MetPro: 1.633 ± 0.039	0.756MetGln: 0.756 ± 0.023	1.905MetArg: 1.905 ± 0.037	1.719MetSer: 1.719 ± 0.033	2.119MetThr: 2.119 ± 0.038	2.027MetVal: 2.027 ± 0.042	0.205MetTrp: 0.205 ± 0.011	0.294MetTyr: 0.294 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	3.023AsnAla: 3.023 ± 0.049	0.223AsnCys: 0.223 ± 0.012	1.22AsnAsp: 1.22 ± 0.034	1.061AsnGlu: 1.061 ± 0.035	0.835AsnPhe: 0.835 ± 0.027	2.177AsnGly: 2.177 ± 0.039	0.46AsnHis: 0.46 ± 0.016	1.221AsnIle: 1.221 ± 0.033	0.566AsnLys: 0.566 ± 0.024	2.421AsnLeu: 2.421 ± 0.044	0.547AsnMet: 0.547 ± 0.019	0.603AsnAsn: 0.603 ± 0.023	1.843AsnPro: 1.843 ± 0.042	0.738AsnGln: 0.738 ± 0.023	1.765AsnArg: 1.765 ± 0.036	1.079AsnSer: 1.079 ± 0.03	1.137AsnThr: 1.137 ± 0.036	1.898AsnVal: 1.898 ± 0.035	0.399AsnTrp: 0.399 ± 0.015	0.544AsnTyr: 0.544 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	7.25ProAla: 7.25 ± 0.11	0.281ProCys: 0.281 ± 0.014	3.649ProAsp: 3.649 ± 0.053	3.214ProGlu: 3.214 ± 0.05	2.133ProPhe: 2.133 ± 0.039	4.965ProGly: 4.965 ± 0.062	1.067ProHis: 1.067 ± 0.028	2.64ProIle: 2.64 ± 0.045	1.693ProLys: 1.693 ± 0.032	4.874ProLeu: 4.874 ± 0.059	1.369ProMet: 1.369 ± 0.031	1.428ProAsn: 1.428 ± 0.031	2.969ProPro: 2.969 ± 0.073	1.706ProGln: 1.706 ± 0.038	3.328ProArg: 3.328 ± 0.049	2.89ProSer: 2.89 ± 0.043	2.803ProThr: 2.803 ± 0.047	4.633ProVal: 4.633 ± 0.068	0.698ProTrp: 0.698 ± 0.023	1.11ProTyr: 1.11 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	4.422GlnAla: 4.422 ± 0.061	0.192GlnCys: 0.192 ± 0.013	1.286GlnAsp: 1.286 ± 0.029	1.227GlnGlu: 1.227 ± 0.031	1.039GlnPhe: 1.039 ± 0.026	2.445GlnGly: 2.445 ± 0.04	0.527GlnHis: 0.527 ± 0.02	1.779GlnIle: 1.779 ± 0.039	0.914GlnLys: 0.914 ± 0.023	2.579GlnLeu: 2.579 ± 0.082	0.855GlnMet: 0.855 ± 0.023	0.787GlnAsn: 0.787 ± 0.026	1.92GlnPro: 1.92 ± 0.041	1.115GlnGln: 1.115 ± 0.035	2.464GlnArg: 2.464 ± 0.045	1.656GlnSer: 1.656 ± 0.035	1.808GlnThr: 1.808 ± 0.032	2.488GlnVal: 2.488 ± 0.084	0.367GlnTrp: 0.367 ± 0.016	0.491GlnTyr: 0.491 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	8.456ArgAla: 8.456 ± 0.087	0.468ArgCys: 0.468 ± 0.016	4.121ArgAsp: 4.121 ± 0.061	3.878ArgGlu: 3.878 ± 0.054	3.211ArgPhe: 3.211 ± 0.05	5.238ArgGly: 5.238 ± 0.071	1.701ArgHis: 1.701 ± 0.033	4.261ArgIle: 4.261 ± 0.058	1.956ArgLys: 1.956 ± 0.038	8.047ArgLeu: 8.047 ± 0.094	2.072ArgMet: 2.072 ± 0.035	1.75ArgAsn: 1.75 ± 0.035	4.088ArgPro: 4.088 ± 0.065	2.583ArgGln: 2.583 ± 0.042	6.015ArgArg: 6.015 ± 0.088	3.887ArgSer: 3.887 ± 0.055	3.662ArgThr: 3.662 ± 0.048	5.052ArgVal: 5.052 ± 0.069	1.035ArgTrp: 1.035 ± 0.024	1.622ArgTyr: 1.622 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	6.376SerAla: 6.376 ± 0.074	0.379SerCys: 0.379 ± 0.017	2.698SerAsp: 2.698 ± 0.041	2.447SerGlu: 2.447 ± 0.037	2.266SerPhe: 2.266 ± 0.041	5.453SerGly: 5.453 ± 0.086	1.0SerHis: 1.0 ± 0.024	2.739SerIle: 2.739 ± 0.04	1.358SerLys: 1.358 ± 0.035	5.414SerLeu: 5.414 ± 0.07	1.323SerMet: 1.323 ± 0.027	1.284SerAsn: 1.284 ± 0.032	2.907SerPro: 2.907 ± 0.055	1.519SerGln: 1.519 ± 0.035	3.703SerArg: 3.703 ± 0.06	2.926SerSer: 2.926 ± 0.07	2.886SerThr: 2.886 ± 0.142	3.867SerVal: 3.867 ± 0.056	0.737SerTrp: 0.737 ± 0.023	1.105SerTyr: 1.105 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	6.626ThrAla: 6.626 ± 0.074	0.435ThrCys: 0.435 ± 0.018	2.792ThrAsp: 2.792 ± 0.045	2.562ThrGlu: 2.562 ± 0.043	2.149ThrPhe: 2.149 ± 0.042	5.369ThrGly: 5.369 ± 0.066	1.012ThrHis: 1.012 ± 0.025	3.252ThrIle: 3.252 ± 0.05	1.414ThrLys: 1.414 ± 0.033	6.022ThrLeu: 6.022 ± 0.066	1.315ThrMet: 1.315 ± 0.034	1.317ThrAsn: 1.317 ± 0.043	3.309ThrPro: 3.309 ± 0.051	1.592ThrGln: 1.592 ± 0.185	3.73ThrArg: 3.73 ± 0.056	3.035ThrSer: 3.035 ± 0.091	3.186ThrThr: 3.186 ± 0.151	4.471ThrVal: 4.471 ± 0.055	0.693ThrTrp: 0.693 ± 0.025	1.259ThrTyr: 1.259 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	10.379ValAla: 10.379 ± 0.102	0.586ValCys: 0.586 ± 0.02	4.322ValAsp: 4.322 ± 0.059	4.414ValGlu: 4.414 ± 0.059	2.722ValPhe: 2.722 ± 0.052	5.885ValGly: 5.885 ± 0.073	1.414ValHis: 1.414 ± 0.031	4.195ValIle: 4.195 ± 0.056	2.328ValLys: 2.328 ± 0.048	7.661ValLeu: 7.661 ± 0.072	2.059ValMet: 2.059 ± 0.041	1.914ValAsn: 1.914 ± 0.04	4.218ValPro: 4.218 ± 0.062	2.106ValGln: 2.106 ± 0.034	5.145ValArg: 5.145 ± 0.061	4.318ValSer: 4.318 ± 0.067	4.965ValThr: 4.965 ± 0.06	6.582ValVal: 6.582 ± 0.079	0.906ValTrp: 0.906 ± 0.027	1.369ValTyr: 1.369 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.225TrpAla: 1.225 ± 0.031	0.134TrpCys: 0.134 ± 0.01	0.561TrpAsp: 0.561 ± 0.019	0.489TrpGlu: 0.489 ± 0.018	0.551TrpPhe: 0.551 ± 0.02	0.992TrpGly: 0.992 ± 0.027	0.283TrpHis: 0.283 ± 0.016	0.656TrpIle: 0.656 ± 0.023	0.409TrpLys: 0.409 ± 0.017	1.509TrpLeu: 1.509 ± 0.037	0.358TrpMet: 0.358 ± 0.016	0.423TrpAsn: 0.423 ± 0.02	0.705TrpPro: 0.705 ± 0.022	0.53TrpGln: 0.53 ± 0.02	1.18TrpArg: 1.18 ± 0.033	0.796TrpSer: 0.796 ± 0.025	0.843TrpThr: 0.843 ± 0.023	0.849TrpVal: 0.849 ± 0.028	0.24TrpTrp: 0.24 ± 0.016	0.254TrpTyr: 0.254 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.356TyrAla: 2.356 ± 0.044	0.213TyrCys: 0.213 ± 0.013	1.25TyrAsp: 1.25 ± 0.034	1.023TyrGlu: 1.023 ± 0.03	0.806TyrPhe: 0.806 ± 0.021	2.089TyrGly: 2.089 ± 0.042	0.372TyrHis: 0.372 ± 0.018	0.705TyrIle: 0.705 ± 0.021	0.519TyrLys: 0.519 ± 0.022	2.11TyrLeu: 2.11 ± 0.038	0.43TyrMet: 0.43 ± 0.018	0.484TyrAsn: 0.484 ± 0.017	1.047TyrPro: 1.047 ± 0.026	0.653TyrGln: 0.653 ± 0.019	1.739TyrArg: 1.739 ± 0.034	1.039TyrSer: 1.039 ± 0.03	1.009TyrThr: 1.009 ± 0.026	1.522TyrVal: 1.522 ± 0.031	0.331TyrTrp: 0.331 ± 0.016	0.446TyrTyr: 0.446 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4643 proteins (1481849 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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