Monoglobus pectinilyticus
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2258 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K9P291|A0A2K9P291_9FIRM GCN5-related N-acetyltransferase OS=Monoglobus pectinilyticus OX=1981510 GN=B9O19_01221 PE=4 SV=1
MM1 pKa = 7.86 RR2 pKa = 11.84 NLKK5 pKa = 10.19 KK6 pKa = 10.71 VIALIAVFAMMVSTVAFAATFGDD29 pKa = 3.96 VKK31 pKa = 11.03 EE32 pKa = 4.18 GDD34 pKa = 3.58 NYY36 pKa = 11.26 YY37 pKa = 10.78 EE38 pKa = 5.57 AIEE41 pKa = 4.18 TLNKK45 pKa = 10.47 LGILTGDD52 pKa = 4.06 DD53 pKa = 3.79 EE54 pKa = 5.62 NNDD57 pKa = 2.72 GVMEE61 pKa = 3.84 YY62 pKa = 10.36 RR63 pKa = 11.84 PNDD66 pKa = 3.91 SITRR70 pKa = 11.84 AEE72 pKa = 3.79 ITVIVARR79 pKa = 11.84 IQGQTGAVAQADD91 pKa = 4.1 TVFTDD96 pKa = 3.55 VPSTYY101 pKa = 9.23 WASGYY106 pKa = 9.59 IASATNQGIINGYY119 pKa = 10.16 GDD121 pKa = 3.86 GTFGPDD127 pKa = 3.96 DD128 pKa = 3.64 KK129 pKa = 11.59 VLYY132 pKa = 10.29 EE133 pKa = 4.67 DD134 pKa = 4.02 VIKK137 pKa = 10.51 MLMEE141 pKa = 3.88 TLGYY145 pKa = 10.01 KK146 pKa = 10.19 PYY148 pKa = 10.43 AQNNGGYY155 pKa = 6.11 PTGYY159 pKa = 9.59 ILAAQRR165 pKa = 11.84 QSVLKK170 pKa = 10.1 NVVGGAEE177 pKa = 4.16 GTEE180 pKa = 3.92 ATRR183 pKa = 11.84 GQVAQMTYY191 pKa = 10.82 NAIDD195 pKa = 3.55 TPLMEE200 pKa = 4.49 RR201 pKa = 11.84 YY202 pKa = 9.9 VYY204 pKa = 10.6 GGEE207 pKa = 3.85 AQYY210 pKa = 10.6 VIWDD214 pKa = 3.91 GEE216 pKa = 4.33 SWSPRR221 pKa = 11.84 KK222 pKa = 8.04 TLMNQALGINKK233 pKa = 9.66 LKK235 pKa = 11.03 GVVTEE240 pKa = 4.51 NEE242 pKa = 4.07 VTALDD247 pKa = 3.76 AAVQIDD253 pKa = 3.98 TDD255 pKa = 3.46 ATQQIKK261 pKa = 10.69 LYY263 pKa = 11.18 VEE265 pKa = 4.57 DD266 pKa = 5.26 NYY268 pKa = 11.37 LGSNDD273 pKa = 3.64 TNSDD277 pKa = 3.88 YY278 pKa = 11.16 EE279 pKa = 4.17 VDD281 pKa = 3.58 SVYY284 pKa = 10.79 PFYY287 pKa = 10.94 TGDD290 pKa = 3.42 TNAADD295 pKa = 3.68 YY296 pKa = 11.09 LGYY299 pKa = 10.82 DD300 pKa = 3.57 VVLYY304 pKa = 10.79 AQDD307 pKa = 3.84 NKK309 pKa = 11.18 NEE311 pKa = 4.1 TDD313 pKa = 3.67 TILSITEE320 pKa = 3.84 ATGKK324 pKa = 9.75 NSKK327 pKa = 10.33 VEE329 pKa = 4.03 FTLDD333 pKa = 3.07 KK334 pKa = 10.83 FNSYY338 pKa = 10.85 DD339 pKa = 3.59 ADD341 pKa = 4.25 TNNLSYY347 pKa = 10.48 MKK349 pKa = 10.72 NDD351 pKa = 3.55 TDD353 pKa = 4.8 KK354 pKa = 11.49 SATKK358 pKa = 10.56 LKK360 pKa = 10.78 LQTTSNRR367 pKa = 11.84 VNYY370 pKa = 9.58 SDD372 pKa = 4.31 SPAIIYY378 pKa = 10.34 NGIAYY383 pKa = 10.3 SGTLEE388 pKa = 4.21 SLFGSYY394 pKa = 10.23 EE395 pKa = 3.91 GDD397 pKa = 3.3 EE398 pKa = 4.39 SGLIYY403 pKa = 10.29 KK404 pKa = 10.58 DD405 pKa = 3.15 SAYY408 pKa = 10.44 SGKK411 pKa = 8.64 VTVLDD416 pKa = 3.92 NDD418 pKa = 3.74 DD419 pKa = 3.47 TSGYY423 pKa = 10.95 DD424 pKa = 3.33 VIFVDD429 pKa = 3.94 VAVGAVVDD437 pKa = 4.18 EE438 pKa = 4.39 LSSRR442 pKa = 11.84 GVLTFKK448 pKa = 11.03 NSVGDD453 pKa = 3.89 RR454 pKa = 11.84 NHH456 pKa = 6.1 MNSVSKK462 pKa = 11.48 VEE464 pKa = 4.22 FDD466 pKa = 4.0 SSNTDD471 pKa = 2.93 MYY473 pKa = 11.5 INLTKK478 pKa = 10.52 DD479 pKa = 3.29 GKK481 pKa = 9.92 PYY483 pKa = 10.8 DD484 pKa = 3.97 YY485 pKa = 9.97 TALKK489 pKa = 10.02 AWDD492 pKa = 3.75 VLSIVANNGDD502 pKa = 4.09 AQSDD506 pKa = 4.89 YY507 pKa = 11.7 YY508 pKa = 10.89 DD509 pKa = 3.89 IKK511 pKa = 11.54 VLDD514 pKa = 3.96 NTNTAITGSVSRR526 pKa = 11.84 TSSSDD531 pKa = 2.89 TSADD535 pKa = 2.94 GTAYY539 pKa = 9.57 TISGVDD545 pKa = 3.24 YY546 pKa = 10.89 DD547 pKa = 4.58 LAEE550 pKa = 4.62 GYY552 pKa = 10.51 YY553 pKa = 11.12 NNGTLKK559 pKa = 10.64 AGSEE563 pKa = 4.13 GTFYY567 pKa = 10.44 IDD569 pKa = 3.22 EE570 pKa = 4.34 YY571 pKa = 11.29 GKK573 pKa = 10.08 IVAYY577 pKa = 10.82 NKK579 pKa = 10.26 DD580 pKa = 4.16 SNSKK584 pKa = 8.9 TSDD587 pKa = 2.89 NYY589 pKa = 11.3 GYY591 pKa = 11.06 VIDD594 pKa = 4.49 AQITSSGFSDD604 pKa = 3.93 NVPSLQIIYY613 pKa = 10.33 KK614 pKa = 10.1 DD615 pKa = 3.81 GTIGTYY621 pKa = 10.49 DD622 pKa = 3.36 FQGTVTIDD630 pKa = 3.23 NPTEE634 pKa = 4.3 EE635 pKa = 4.17 IQKK638 pKa = 10.62 LVGDD642 pKa = 4.26 NSDD645 pKa = 3.64 SVSFKK650 pKa = 11.04 YY651 pKa = 10.3 KK652 pKa = 10.38 DD653 pKa = 3.62 YY654 pKa = 11.38 SVDD657 pKa = 3.49 AMTEE661 pKa = 4.21 AINTMAGTVITYY673 pKa = 9.32 NATNGYY679 pKa = 9.0 IKK681 pKa = 10.36 SITMAALNSTTDD693 pKa = 3.18 DD694 pKa = 3.51 STLVLEE700 pKa = 5.1 KK701 pKa = 10.71 ADD703 pKa = 3.84 TANVSSFDD711 pKa = 3.84 EE712 pKa = 4.1 DD713 pKa = 3.72 TKK715 pKa = 10.62 QIRR718 pKa = 11.84 VGGTKK723 pKa = 10.02 KK724 pKa = 10.75 YY725 pKa = 10.95 DD726 pKa = 3.41 VADD729 pKa = 3.87 DD730 pKa = 3.68 TLIFYY735 pKa = 10.09 IGKK738 pKa = 9.03 PGDD741 pKa = 3.47 SFVYY745 pKa = 9.97 NKK747 pKa = 9.44 PAAGEE752 pKa = 3.84 PSEE755 pKa = 4.1 NCIVGQGSEE764 pKa = 4.76 LRR766 pKa = 11.84 TNDD769 pKa = 3.33 NFSSAVYY776 pKa = 10.47 RR777 pKa = 11.84 NGDD780 pKa = 3.33 EE781 pKa = 4.33 SGTASVIVMYY791 pKa = 9.24 NTDD794 pKa = 3.85 PGVGASTGVAYY805 pKa = 8.63 ITSIGQTTVDD815 pKa = 3.36 GSKK818 pKa = 10.45 VLSITYY824 pKa = 9.63 YY825 pKa = 10.27 QDD827 pKa = 4.02 GEE829 pKa = 4.28 LKK831 pKa = 10.37 EE832 pKa = 5.29 ANTDD836 pKa = 3.53 DD837 pKa = 4.15 MSVGDD842 pKa = 4.75 YY843 pKa = 11.09 LDD845 pKa = 3.55 QNTVAGSLFKK855 pKa = 10.86 FGISNGTITSAVPYY869 pKa = 8.85 LTFDD873 pKa = 3.08 GTVRR877 pKa = 11.84 DD878 pKa = 3.94 KK879 pKa = 11.37 VYY881 pKa = 10.86 EE882 pKa = 4.32 GDD884 pKa = 4.0 DD885 pKa = 3.59 FTTAGIPNANYY896 pKa = 10.51 VKK898 pKa = 8.34 TTGSNSDD905 pKa = 3.33 EE906 pKa = 3.97 EE907 pKa = 4.93 VYY909 pKa = 11.0 YY910 pKa = 10.93 GAVVQKK916 pKa = 10.42 SGNRR920 pKa = 11.84 LTIAQADD927 pKa = 3.56 ADD929 pKa = 4.89 GIISLDD935 pKa = 3.64 PNDD938 pKa = 4.12 WEE940 pKa = 4.74 SVSLSNYY947 pKa = 5.89 TANYY951 pKa = 9.27 YY952 pKa = 10.76 LYY954 pKa = 10.74 DD955 pKa = 3.39 PTRR958 pKa = 11.84 NGKK961 pKa = 9.81 AKK963 pKa = 10.32 FQTASLGDD971 pKa = 3.41 ITVDD975 pKa = 3.71 KK976 pKa = 11.23 VLANDD981 pKa = 4.64 GGNGSYY987 pKa = 10.34 TIDD990 pKa = 3.37 FGGVVEE996 pKa = 4.8 DD997 pKa = 4.67 APAFGMLDD1005 pKa = 3.47 FVYY1008 pKa = 10.54 VRR1010 pKa = 11.84 TYY1012 pKa = 10.86 EE1013 pKa = 3.96 RR1014 pKa = 11.84 TADD1017 pKa = 3.62 VVDD1020 pKa = 3.85 YY1021 pKa = 7.48 TTWEE1025 pKa = 3.67 YY1026 pKa = 11.31 DD1027 pKa = 3.66 YY1028 pKa = 11.39 NFSKK1032 pKa = 11.14
Molecular weight: 111.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.986
IPC_protein 4.024
Toseland 3.795
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.948
Rodwell 3.846
Grimsley 3.706
Solomon 4.012
Lehninger 3.973
Nozaki 4.113
DTASelect 4.393
Thurlkill 3.846
EMBOSS 3.961
Sillero 4.139
Patrickios 1.367
IPC_peptide 4.012
IPC2_peptide 4.126
IPC2.peptide.svr19 4.011
Protein with the highest isoelectric point:
>tr|A0A2K9P241|A0A2K9P241_9FIRM Phage protein OS=Monoglobus pectinilyticus OX=1981510 GN=B9O19_00475 PE=4 SV=1
MM1 pKa = 7.45 LRR3 pKa = 11.84 TYY5 pKa = 9.82 QPKK8 pKa = 9.51 KK9 pKa = 7.62 RR10 pKa = 11.84 HH11 pKa = 5.45 RR12 pKa = 11.84 SKK14 pKa = 10.49 VHH16 pKa = 5.68 GFRR19 pKa = 11.84 KK20 pKa = 10.02 RR21 pKa = 11.84 MSTKK25 pKa = 10.18 NGRR28 pKa = 11.84 KK29 pKa = 8.44 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.63 GRR39 pKa = 11.84 KK40 pKa = 8.9 SLSAA44 pKa = 3.86
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2258
0
2258
762331
29
3174
337.6
37.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.719 ± 0.053
1.385 ± 0.025
6.225 ± 0.04
7.043 ± 0.054
4.046 ± 0.036
7.124 ± 0.05
1.339 ± 0.018
8.157 ± 0.052
7.294 ± 0.051
7.886 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.634 ± 0.024
5.945 ± 0.047
3.326 ± 0.039
2.611 ± 0.03
3.69 ± 0.043
6.891 ± 0.053
5.741 ± 0.07
6.722 ± 0.041
0.823 ± 0.019
4.398 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here