Brevibacterium ravenspurgense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Brevibacteriaceae; Brevibacterium

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2020 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A150H5V9|A0A150H5V9_9MICO Beta sliding clamp OS=Brevibacterium ravenspurgense OX=479117 GN=dnaN PE=3 SV=1
MM1 pKa = 7.47ATTTINVSGLTCNHH15 pKa = 6.41CVNAVKK21 pKa = 10.82DD22 pKa = 3.85EE23 pKa = 4.05VGAIDD28 pKa = 4.61GVQSVNVEE36 pKa = 4.06LVEE39 pKa = 4.5GGISPVTIEE48 pKa = 4.25SATPLAEE55 pKa = 5.13ADD57 pKa = 3.92LNDD60 pKa = 5.27AIDD63 pKa = 3.57EE64 pKa = 4.42AGYY67 pKa = 9.03TIVKK71 pKa = 9.99

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A150H5R0|A0A150H5R0_9MICO Malate synthase OS=Brevibacterium ravenspurgense OX=479117 GN=aceB_2 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 8.98RR13 pKa = 11.84AKK15 pKa = 8.49THH17 pKa = 5.22GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SVLAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.74GRR40 pKa = 11.84ANLSAA45 pKa = 4.66

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2020

0

2020

687623

29

1636

340.4

36.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.162 ± 0.077

0.597 ± 0.014

6.26 ± 0.052

6.417 ± 0.052

3.344 ± 0.032

8.736 ± 0.049

1.991 ± 0.02

4.892 ± 0.037

3.379 ± 0.044

9.331 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.018

2.426 ± 0.031

5.156 ± 0.041

3.313 ± 0.031

6.357 ± 0.053

6.06 ± 0.033

5.92 ± 0.035

8.131 ± 0.044

1.301 ± 0.02

2.125 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski