Amino acid dipepetide frequency for Brevibacterium ravenspurgense

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.71AlaAla: 17.71 ± 0.228	0.721AlaCys: 0.721 ± 0.03	8.343AlaAsp: 8.343 ± 0.133	9.101AlaGlu: 9.101 ± 0.158	3.551AlaPhe: 3.551 ± 0.069	11.54AlaGly: 11.54 ± 0.177	2.475AlaHis: 2.475 ± 0.07	5.07AlaIle: 5.07 ± 0.114	4.03AlaLys: 4.03 ± 0.107	11.474AlaLeu: 11.474 ± 0.161	2.56AlaMet: 2.56 ± 0.065	2.302AlaAsn: 2.302 ± 0.056	5.174AlaPro: 5.174 ± 0.106	4.824AlaGln: 4.824 ± 0.092	6.876AlaArg: 6.876 ± 0.113	6.326AlaSer: 6.326 ± 0.111	4.994AlaThr: 4.994 ± 0.091	10.741AlaVal: 10.741 ± 0.167	1.414AlaTrp: 1.414 ± 0.049	2.389AlaTyr: 2.389 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.781CysAla: 0.781 ± 0.032	0.071CysCys: 0.071 ± 0.011	0.369CysAsp: 0.369 ± 0.024	0.33CysGlu: 0.33 ± 0.025	0.192CysPhe: 0.192 ± 0.016	0.649CysGly: 0.649 ± 0.027	0.096CysHis: 0.096 ± 0.013	0.262CysIle: 0.262 ± 0.021	0.106CysLys: 0.106 ± 0.011	0.502CysLeu: 0.502 ± 0.028	0.119CysMet: 0.119 ± 0.012	0.145CysAsn: 0.145 ± 0.013	0.355CysPro: 0.355 ± 0.024	0.143CysGln: 0.143 ± 0.014	0.377CysArg: 0.377 ± 0.024	0.433CysSer: 0.433 ± 0.03	0.364CysThr: 0.364 ± 0.024	0.47CysVal: 0.47 ± 0.026	0.073CysTrp: 0.073 ± 0.01	0.129CysTyr: 0.129 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	6.88AspAla: 6.88 ± 0.114	0.324AspCys: 0.324 ± 0.023	4.348AspAsp: 4.348 ± 0.118	4.569AspGlu: 4.569 ± 0.096	2.227AspPhe: 2.227 ± 0.056	5.625AspGly: 5.625 ± 0.114	1.318AspHis: 1.318 ± 0.047	3.213AspIle: 3.213 ± 0.069	2.119AspLys: 2.119 ± 0.094	5.593AspLeu: 5.593 ± 0.1	1.21AspMet: 1.21 ± 0.047	1.505AspAsn: 1.505 ± 0.062	3.631AspPro: 3.631 ± 0.08	1.709AspGln: 1.709 ± 0.05	3.787AspArg: 3.787 ± 0.081	3.727AspSer: 3.727 ± 0.083	3.288AspThr: 3.288 ± 0.073	5.112AspVal: 5.112 ± 0.099	0.871AspTrp: 0.871 ± 0.034	1.544AspTyr: 1.544 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	8.131GluAla: 8.131 ± 0.142	0.33GluCys: 0.33 ± 0.024	4.283GluAsp: 4.283 ± 0.087	4.199GluGlu: 4.199 ± 0.099	2.112GluPhe: 2.112 ± 0.062	4.686GluGly: 4.686 ± 0.094	1.667GluHis: 1.667 ± 0.054	3.105GluIle: 3.105 ± 0.078	2.446GluLys: 2.446 ± 0.078	6.437GluLeu: 6.437 ± 0.118	1.015GluMet: 1.015 ± 0.038	1.838GluAsn: 1.838 ± 0.05	3.085GluPro: 3.085 ± 0.1	2.632GluGln: 2.632 ± 0.065	4.406GluArg: 4.406 ± 0.091	3.39GluSer: 3.39 ± 0.085	3.178GluThr: 3.178 ± 0.07	4.846GluVal: 4.846 ± 0.084	0.874GluTrp: 0.874 ± 0.039	1.511GluTyr: 1.511 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	4.21PheAla: 4.21 ± 0.091	0.253PheCys: 0.253 ± 0.02	2.388PheAsp: 2.388 ± 0.063	1.825PheGlu: 1.825 ± 0.058	1.341PhePhe: 1.341 ± 0.061	3.578PheGly: 3.578 ± 0.072	0.569PheHis: 0.569 ± 0.029	1.659PheIle: 1.659 ± 0.055	0.865PheLys: 0.865 ± 0.037	2.833PheLeu: 2.833 ± 0.08	0.638PheMet: 0.638 ± 0.032	0.896PheAsn: 0.896 ± 0.036	1.507PhePro: 1.507 ± 0.049	0.848PheGln: 0.848 ± 0.036	1.655PheArg: 1.655 ± 0.052	2.321PheSer: 2.321 ± 0.058	2.35PheThr: 2.35 ± 0.059	2.504PheVal: 2.504 ± 0.07	0.455PheTrp: 0.455 ± 0.029	0.74PheTyr: 0.74 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	9.335GlyAla: 9.335 ± 0.151	0.595GlyCys: 0.595 ± 0.033	4.854GlyAsp: 4.854 ± 0.101	5.195GlyGlu: 5.195 ± 0.092	3.365GlyPhe: 3.365 ± 0.074	7.33GlyGly: 7.33 ± 0.152	1.799GlyHis: 1.799 ± 0.058	4.731GlyIle: 4.731 ± 0.094	3.496GlyLys: 3.496 ± 0.094	8.538GlyLeu: 8.538 ± 0.123	2.12GlyMet: 2.12 ± 0.06	1.946GlyAsn: 1.946 ± 0.068	3.607GlyPro: 3.607 ± 0.083	3.06GlyGln: 3.06 ± 0.075	5.5GlyArg: 5.5 ± 0.106	5.795GlySer: 5.795 ± 0.105	5.676GlyThr: 5.676 ± 0.098	6.882GlyVal: 6.882 ± 0.113	1.395GlyTrp: 1.395 ± 0.046	2.147GlyTyr: 2.147 ± 0.054	0.0GlyXaa: 0.0 ± 0.0
His	2.106HisAla: 2.106 ± 0.062	0.127HisCys: 0.127 ± 0.013	1.188HisAsp: 1.188 ± 0.046	1.142HisGlu: 1.142 ± 0.042	0.637HisPhe: 0.637 ± 0.033	1.831HisGly: 1.831 ± 0.055	0.476HisHis: 0.476 ± 0.028	0.967HisIle: 0.967 ± 0.04	0.484HisLys: 0.484 ± 0.028	1.827HisLeu: 1.827 ± 0.055	0.422HisMet: 0.422 ± 0.025	0.494HisAsn: 0.494 ± 0.026	1.361HisPro: 1.361 ± 0.045	0.567HisGln: 0.567 ± 0.032	1.485HisArg: 1.485 ± 0.047	1.267HisSer: 1.267 ± 0.046	1.243HisThr: 1.243 ± 0.046	1.528HisVal: 1.528 ± 0.046	0.272HisTrp: 0.272 ± 0.021	0.483HisTyr: 0.483 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.364IleAla: 6.364 ± 0.11	0.321IleCys: 0.321 ± 0.019	3.592IleAsp: 3.592 ± 0.082	3.191IleGlu: 3.191 ± 0.072	1.54IlePhe: 1.54 ± 0.056	4.806IleGly: 4.806 ± 0.097	0.855IleHis: 0.855 ± 0.037	2.69IleIle: 2.69 ± 0.079	1.328IleLys: 1.328 ± 0.042	3.791IleLeu: 3.791 ± 0.097	0.958IleMet: 0.958 ± 0.043	1.37IleAsn: 1.37 ± 0.042	2.461IlePro: 2.461 ± 0.063	1.06IleGln: 1.06 ± 0.042	2.769IleArg: 2.769 ± 0.074	2.813IleSer: 2.813 ± 0.075	3.169IleThr: 3.169 ± 0.075	4.543IleVal: 4.543 ± 0.088	0.423IleTrp: 0.423 ± 0.028	0.873IleTyr: 0.873 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	3.991LysAla: 3.991 ± 0.104	0.131LysCys: 0.131 ± 0.013	2.154LysAsp: 2.154 ± 0.094	1.803LysGlu: 1.803 ± 0.062	0.838LysPhe: 0.838 ± 0.038	2.346LysGly: 2.346 ± 0.064	0.769LysHis: 0.769 ± 0.038	1.626LysIle: 1.626 ± 0.057	1.859LysLys: 1.859 ± 0.077	3.074LysLeu: 3.074 ± 0.072	0.686LysMet: 0.686 ± 0.041	1.178LysAsn: 1.178 ± 0.048	2.195LysPro: 2.195 ± 0.09	1.307LysGln: 1.307 ± 0.039	2.334LysArg: 2.334 ± 0.06	1.94LysSer: 1.94 ± 0.057	2.056LysThr: 2.056 ± 0.068	2.436LysVal: 2.436 ± 0.064	0.365LysTrp: 0.365 ± 0.026	0.7LysTyr: 0.7 ± 0.039	0.0LysXaa: 0.0 ± 0.0
Leu	11.54LeuAla: 11.54 ± 0.168	0.579LeuCys: 0.579 ± 0.027	5.915LeuAsp: 5.915 ± 0.102	5.519LeuGlu: 5.519 ± 0.1	2.968LeuPhe: 2.968 ± 0.077	8.131LeuGly: 8.131 ± 0.12	1.693LeuHis: 1.693 ± 0.055	4.529LeuIle: 4.529 ± 0.087	2.907LeuLys: 2.907 ± 0.075	8.769LeuLeu: 8.769 ± 0.161	2.051LeuMet: 2.051 ± 0.053	2.264LeuAsn: 2.264 ± 0.062	5.241LeuPro: 5.241 ± 0.078	2.567LeuGln: 2.567 ± 0.065	6.331LeuArg: 6.331 ± 0.114	5.952LeuSer: 5.952 ± 0.093	6.264LeuThr: 6.264 ± 0.104	7.363LeuVal: 7.363 ± 0.129	1.099LeuTrp: 1.099 ± 0.042	1.632LeuTyr: 1.632 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	2.395MetAla: 2.395 ± 0.058	0.161MetCys: 0.161 ± 0.015	1.001MetAsp: 1.001 ± 0.04	1.049MetGlu: 1.049 ± 0.043	0.707MetPhe: 0.707 ± 0.029	1.687MetGly: 1.687 ± 0.055	0.41MetHis: 0.41 ± 0.023	0.98MetIle: 0.98 ± 0.039	0.794MetLys: 0.794 ± 0.027	2.045MetLeu: 2.045 ± 0.062	0.385MetMet: 0.385 ± 0.027	0.617MetAsn: 0.617 ± 0.028	1.118MetPro: 1.118 ± 0.043	0.63MetGln: 0.63 ± 0.036	1.499MetArg: 1.499 ± 0.045	1.739MetSer: 1.739 ± 0.051	1.793MetThr: 1.793 ± 0.048	1.4MetVal: 1.4 ± 0.042	0.263MetTrp: 0.263 ± 0.018	0.378MetTyr: 0.378 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.693AsnAla: 2.693 ± 0.066	0.151AsnCys: 0.151 ± 0.014	1.262AsnAsp: 1.262 ± 0.046	1.204AsnGlu: 1.204 ± 0.05	0.779AsnPhe: 0.779 ± 0.034	1.994AsnGly: 1.994 ± 0.061	0.433AsnHis: 0.433 ± 0.026	1.268AsnIle: 1.268 ± 0.044	0.793AsnLys: 0.793 ± 0.038	2.336AsnLeu: 2.336 ± 0.063	0.521AsnMet: 0.521 ± 0.027	0.65AsnAsn: 0.65 ± 0.039	1.985AsnPro: 1.985 ± 0.059	0.736AsnGln: 0.736 ± 0.034	1.659AsnArg: 1.659 ± 0.057	1.491AsnSer: 1.491 ± 0.042	1.611AsnThr: 1.611 ± 0.047	1.799AsnVal: 1.799 ± 0.056	0.311AsnTrp: 0.311 ± 0.021	0.58AsnTyr: 0.58 ± 0.033	0.0AsnXaa: 0.0 ± 0.0
Pro	6.32ProAla: 6.32 ± 0.117	0.211ProCys: 0.211 ± 0.018	3.505ProAsp: 3.505 ± 0.086	4.444ProGlu: 4.444 ± 0.093	1.579ProPhe: 1.579 ± 0.049	4.899ProGly: 4.899 ± 0.11	1.107ProHis: 1.107 ± 0.046	2.068ProIle: 2.068 ± 0.055	1.879ProLys: 1.879 ± 0.062	4.225ProLeu: 4.225 ± 0.074	0.957ProMet: 0.957 ± 0.039	1.37ProAsn: 1.37 ± 0.045	1.854ProPro: 1.854 ± 0.064	2.088ProGln: 2.088 ± 0.057	2.743ProArg: 2.743 ± 0.073	3.012ProSer: 3.012 ± 0.079	3.182ProThr: 3.182 ± 0.079	4.412ProVal: 4.412 ± 0.079	0.675ProTrp: 0.675 ± 0.037	1.03ProTyr: 1.03 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	4.295GlnAla: 4.295 ± 0.098	0.138GlnCys: 0.138 ± 0.015	1.457GlnAsp: 1.457 ± 0.053	1.767GlnGlu: 1.767 ± 0.054	0.992GlnPhe: 0.992 ± 0.041	2.059GlnGly: 2.059 ± 0.06	0.742GlnHis: 0.742 ± 0.035	1.683GlnIle: 1.683 ± 0.05	1.438GlnLys: 1.438 ± 0.05	3.447GlnLeu: 3.447 ± 0.071	0.806GlnMet: 0.806 ± 0.033	0.862GlnAsn: 0.862 ± 0.037	2.019GlnPro: 2.019 ± 0.074	1.636GlnGln: 1.636 ± 0.067	2.546GlnArg: 2.546 ± 0.069	1.69GlnSer: 1.69 ± 0.05	1.949GlnThr: 1.949 ± 0.058	2.411GlnVal: 2.411 ± 0.06	0.553GlnTrp: 0.553 ± 0.031	0.638GlnTyr: 0.638 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	7.03ArgAla: 7.03 ± 0.122	0.369ArgCys: 0.369 ± 0.023	3.48ArgAsp: 3.48 ± 0.078	4.398ArgGlu: 4.398 ± 0.093	2.228ArgPhe: 2.228 ± 0.058	4.994ArgGly: 4.994 ± 0.09	1.149ArgHis: 1.149 ± 0.038	3.335ArgIle: 3.335 ± 0.065	2.193ArgLys: 2.193 ± 0.06	6.085ArgLeu: 6.085 ± 0.112	1.611ArgMet: 1.611 ± 0.046	1.384ArgAsn: 1.384 ± 0.049	3.093ArgPro: 3.093 ± 0.069	2.093ArgGln: 2.093 ± 0.057	5.163ArgArg: 5.163 ± 0.117	3.969ArgSer: 3.969 ± 0.078	3.986ArgThr: 3.986 ± 0.071	4.646ArgVal: 4.646 ± 0.09	0.857ArgTrp: 0.857 ± 0.035	1.507ArgTyr: 1.507 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	7.83SerAla: 7.83 ± 0.13	0.343SerCys: 0.343 ± 0.026	3.554SerAsp: 3.554 ± 0.073	3.659SerGlu: 3.659 ± 0.09	2.072SerPhe: 2.072 ± 0.057	6.022SerGly: 6.022 ± 0.092	1.124SerHis: 1.124 ± 0.038	2.672SerIle: 2.672 ± 0.06	1.863SerLys: 1.863 ± 0.057	5.314SerLeu: 5.314 ± 0.095	1.412SerMet: 1.412 ± 0.052	1.347SerAsn: 1.347 ± 0.044	3.057SerPro: 3.057 ± 0.068	1.973SerGln: 1.973 ± 0.069	3.883SerArg: 3.883 ± 0.092	3.908SerSer: 3.908 ± 0.081	3.697SerThr: 3.697 ± 0.082	4.693SerVal: 4.693 ± 0.096	0.811SerTrp: 0.811 ± 0.035	1.36SerTyr: 1.36 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	7.858ThrAla: 7.858 ± 0.132	0.307ThrCys: 0.307 ± 0.02	3.534ThrAsp: 3.534 ± 0.085	3.476ThrGlu: 3.476 ± 0.083	1.838ThrPhe: 1.838 ± 0.051	5.701ThrGly: 5.701 ± 0.103	1.11ThrHis: 1.11 ± 0.039	2.955ThrIle: 2.955 ± 0.07	1.741ThrLys: 1.741 ± 0.059	4.99ThrLeu: 4.99 ± 0.094	1.145ThrMet: 1.145 ± 0.04	1.264ThrAsn: 1.264 ± 0.044	3.669ThrPro: 3.669 ± 0.085	1.812ThrGln: 1.812 ± 0.052	3.058ThrArg: 3.058 ± 0.067	3.627ThrSer: 3.627 ± 0.074	3.291ThrThr: 3.291 ± 0.072	5.859ThrVal: 5.859 ± 0.107	0.749ThrTrp: 0.749 ± 0.035	1.214ThrTyr: 1.214 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	8.273ValAla: 8.273 ± 0.147	0.59ValCys: 0.59 ± 0.036	5.227ValAsp: 5.227 ± 0.083	5.323ValGlu: 5.323 ± 0.1	3.125ValPhe: 3.125 ± 0.074	6.556ValGly: 6.556 ± 0.11	1.515ValHis: 1.515 ± 0.05	4.559ValIle: 4.559 ± 0.09	2.394ValLys: 2.394 ± 0.069	8.563ValLeu: 8.563 ± 0.126	1.658ValMet: 1.658 ± 0.053	1.928ValAsn: 1.928 ± 0.052	4.489ValPro: 4.489 ± 0.083	2.296ValGln: 2.296 ± 0.055	5.094ValArg: 5.094 ± 0.103	5.029ValSer: 5.029 ± 0.08	4.975ValThr: 4.975 ± 0.097	7.072ValVal: 7.072 ± 0.126	1.035ValTrp: 1.035 ± 0.041	1.604ValTyr: 1.604 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	1.495TrpAla: 1.495 ± 0.054	0.093TrpCys: 0.093 ± 0.012	0.745TrpAsp: 0.745 ± 0.031	0.682TrpGlu: 0.682 ± 0.032	0.521TrpPhe: 0.521 ± 0.032	0.922TrpGly: 0.922 ± 0.037	0.276TrpHis: 0.276 ± 0.019	0.673TrpIle: 0.673 ± 0.033	0.493TrpLys: 0.493 ± 0.028	1.478TrpLeu: 1.478 ± 0.054	0.339TrpMet: 0.339 ± 0.021	0.417TrpAsn: 0.417 ± 0.026	0.608TrpPro: 0.608 ± 0.032	0.509TrpGln: 0.509 ± 0.031	0.765TrpArg: 0.765 ± 0.032	0.697TrpSer: 0.697 ± 0.033	0.795TrpThr: 0.795 ± 0.034	0.967TrpVal: 0.967 ± 0.046	0.244TrpTrp: 0.244 ± 0.02	0.289TrpTyr: 0.289 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.379TyrAla: 2.379 ± 0.056	0.148TyrCys: 0.148 ± 0.015	1.402TyrAsp: 1.402 ± 0.052	1.294TyrGlu: 1.294 ± 0.052	0.823TyrPhe: 0.823 ± 0.034	2.01TyrGly: 2.01 ± 0.058	0.324TyrHis: 0.324 ± 0.023	0.874TyrIle: 0.874 ± 0.041	0.56TyrLys: 0.56 ± 0.027	1.994TyrLeu: 1.994 ± 0.056	0.438TyrMet: 0.438 ± 0.025	0.478TyrAsn: 0.478 ± 0.025	1.051TyrPro: 1.051 ± 0.042	0.637TyrGln: 0.637 ± 0.031	1.639TyrArg: 1.639 ± 0.051	1.47TyrSer: 1.47 ± 0.046	1.335TyrThr: 1.335 ± 0.046	1.622TyrVal: 1.622 ± 0.048	0.269TyrTrp: 0.269 ± 0.021	0.5TyrTyr: 0.5 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2020 proteins (687623 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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