Flavobacterium phage vB_FspS_hemulen9-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lillamyvirus; unclassified Lillamyvirus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9LA75|A0A6B9LA75_9CAUD Uncharacterized protein OS=Flavobacterium phage vB_FspS_hemulen9-1 OX=2686249 GN=hemulen91_gp021 PE=4 SV=1
MM1 pKa = 7.13TKK3 pKa = 10.25FVIMKK8 pKa = 7.91TLKK11 pKa = 10.69LLLITALAILFLSCSNDD28 pKa = 3.34DD29 pKa = 4.75NEE31 pKa = 4.51QCTYY35 pKa = 9.76TCNAWVKK42 pKa = 10.29PDD44 pKa = 3.4GQIRR48 pKa = 11.84TVIPVEE54 pKa = 4.25LNCEE58 pKa = 4.08TNEE61 pKa = 4.67PINLPEE67 pKa = 4.47GYY69 pKa = 9.92IFLGCDD75 pKa = 3.18NDD77 pKa = 4.19NIPP80 pKa = 4.55

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LAT3|A0A6B9LAT3_9CAUD Uncharacterized protein OS=Flavobacterium phage vB_FspS_hemulen9-1 OX=2686249 GN=hemulen91_gp029 PE=4 SV=1
MM1 pKa = 7.84WSRR4 pKa = 11.84NSEE7 pKa = 4.15SFSFRR12 pKa = 11.84LIYY15 pKa = 9.77RR16 pKa = 11.84HH17 pKa = 5.53KK18 pKa = 10.85NKK20 pKa = 10.48YY21 pKa = 10.1KK22 pKa = 10.83LITNATIAYY31 pKa = 7.4NRR33 pKa = 11.84CC34 pKa = 3.15

Molecular weight: 4.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

12195

31

930

174.2

20.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.887 ± 0.404

1.14 ± 0.146

5.609 ± 0.274

7.306 ± 0.305

5.125 ± 0.258

4.699 ± 0.253

1.369 ± 0.162

7.995 ± 0.266

9.512 ± 0.506

8.856 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.173 ± 0.145

7.995 ± 0.284

2.558 ± 0.182

3.911 ± 0.329

3.018 ± 0.164

6.445 ± 0.268

6.535 ± 0.375

5.527 ± 0.227

0.992 ± 0.111

4.346 ± 0.273
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski