Amino acid dipepetide frequency for Flavobacterium phage vB_FspS_hemulen9-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.738AlaAla: 0.738 ± 0.306
0.656AlaCys: 0.656 ± 0.23
1.968AlaAsp: 1.968 ± 0.357
2.706AlaGlu: 2.706 ± 0.61
2.214AlaPhe: 2.214 ± 0.475
2.132AlaGly: 2.132 ± 0.403
0.656AlaHis: 0.656 ± 0.281
4.018AlaIle: 4.018 ± 0.525
5.659AlaLys: 5.659 ± 0.646
4.674AlaLeu: 4.674 ± 0.729
1.23AlaMet: 1.23 ± 0.413
4.92AlaAsn: 4.92 ± 0.736
0.902AlaPro: 0.902 ± 0.276
1.886AlaGln: 1.886 ± 0.352
1.64AlaArg: 1.64 ± 0.441
3.116AlaSer: 3.116 ± 0.628
3.936AlaThr: 3.936 ± 0.569
3.28AlaVal: 3.28 ± 0.594
0.492AlaTrp: 0.492 ± 0.169
2.05AlaTyr: 2.05 ± 0.363
0.0AlaXaa: 0.0 ± 0.0
Cys
0.246CysAla: 0.246 ± 0.173
0.246CysCys: 0.246 ± 0.148
0.82CysAsp: 0.82 ± 0.237
1.148CysGlu: 1.148 ± 0.328
0.656CysPhe: 0.656 ± 0.238
0.984CysGly: 0.984 ± 0.333
0.082CysHis: 0.082 ± 0.071
0.328CysIle: 0.328 ± 0.161
1.066CysLys: 1.066 ± 0.369
1.312CysLeu: 1.312 ± 0.333
0.164CysMet: 0.164 ± 0.106
0.41CysAsn: 0.41 ± 0.217
0.656CysPro: 0.656 ± 0.245
0.246CysGln: 0.246 ± 0.128
0.246CysArg: 0.246 ± 0.15
0.902CysSer: 0.902 ± 0.291
0.574CysThr: 0.574 ± 0.182
0.738CysVal: 0.738 ± 0.223
0.164CysTrp: 0.164 ± 0.108
0.41CysTyr: 0.41 ± 0.183
0.0CysXaa: 0.0 ± 0.0
Asp
3.772AspAla: 3.772 ± 0.436
1.066AspCys: 1.066 ± 0.285
1.886AspAsp: 1.886 ± 0.438
4.346AspGlu: 4.346 ± 0.705
3.854AspPhe: 3.854 ± 0.563
2.706AspGly: 2.706 ± 0.472
0.574AspHis: 0.574 ± 0.23
3.772AspIle: 3.772 ± 0.558
5.905AspLys: 5.905 ± 0.829
5.248AspLeu: 5.248 ± 0.517
1.558AspMet: 1.558 ± 0.442
4.592AspAsn: 4.592 ± 0.608
0.492AspPro: 0.492 ± 0.188
0.492AspGln: 0.492 ± 0.226
1.476AspArg: 1.476 ± 0.29
3.69AspSer: 3.69 ± 0.526
3.772AspThr: 3.772 ± 0.583
2.952AspVal: 2.952 ± 0.549
0.738AspTrp: 0.738 ± 0.183
3.198AspTyr: 3.198 ± 0.54
0.0AspXaa: 0.0 ± 0.0
Glu
2.378GluAla: 2.378 ± 0.651
0.902GluCys: 0.902 ± 0.348
3.28GluAsp: 3.28 ± 0.592
4.1GluGlu: 4.1 ± 0.695
4.756GluPhe: 4.756 ± 0.803
2.132GluGly: 2.132 ± 0.4
1.066GluHis: 1.066 ± 0.244
7.545GluIle: 7.545 ± 0.783
7.381GluLys: 7.381 ± 0.698
7.463GluLeu: 7.463 ± 0.842
1.968GluMet: 1.968 ± 0.362
6.807GluAsn: 6.807 ± 0.848
1.804GluPro: 1.804 ± 0.36
3.28GluGln: 3.28 ± 0.456
2.296GluArg: 2.296 ± 0.516
3.526GluSer: 3.526 ± 0.478
4.264GluThr: 4.264 ± 0.522
4.182GluVal: 4.182 ± 0.589
0.41GluTrp: 0.41 ± 0.149
3.526GluTyr: 3.526 ± 0.584
0.0GluXaa: 0.0 ± 0.0
Phe
2.542PheAla: 2.542 ± 0.447
0.738PheCys: 0.738 ± 0.249
3.69PheAsp: 3.69 ± 0.516
4.346PheGlu: 4.346 ± 0.619
2.296PhePhe: 2.296 ± 0.367
2.87PheGly: 2.87 ± 0.571
0.492PheHis: 0.492 ± 0.189
3.198PheIle: 3.198 ± 0.462
4.346PheLys: 4.346 ± 0.655
3.444PheLeu: 3.444 ± 0.446
1.312PheMet: 1.312 ± 0.307
4.756PheAsn: 4.756 ± 0.781
0.902PhePro: 0.902 ± 0.243
1.64PheGln: 1.64 ± 0.413
1.394PheArg: 1.394 ± 0.423
3.936PheSer: 3.936 ± 0.651
4.346PheThr: 4.346 ± 0.652
2.624PheVal: 2.624 ± 0.476
0.492PheTrp: 0.492 ± 0.212
1.886PheTyr: 1.886 ± 0.349
0.0PheXaa: 0.0 ± 0.0
Gly
3.034GlyAla: 3.034 ± 0.581
0.328GlyCys: 0.328 ± 0.153
2.788GlyAsp: 2.788 ± 0.563
2.132GlyGlu: 2.132 ± 0.439
2.542GlyPhe: 2.542 ± 0.549
2.296GlyGly: 2.296 ± 0.663
0.328GlyHis: 0.328 ± 0.145
4.018GlyIle: 4.018 ± 0.6
3.198GlyLys: 3.198 ± 0.601
3.526GlyLeu: 3.526 ± 0.506
1.476GlyMet: 1.476 ± 0.311
4.756GlyAsn: 4.756 ± 0.579
0.0GlyPro: 0.0 ± 0.0
1.64GlyGln: 1.64 ± 0.361
1.722GlyArg: 1.722 ± 0.345
3.034GlySer: 3.034 ± 0.45
4.674GlyThr: 4.674 ± 0.839
2.788GlyVal: 2.788 ± 0.418
0.328GlyTrp: 0.328 ± 0.198
2.378GlyTyr: 2.378 ± 0.475
0.0GlyXaa: 0.0 ± 0.0
His
0.41HisAla: 0.41 ± 0.176
0.246HisCys: 0.246 ± 0.144
0.984HisAsp: 0.984 ± 0.307
0.492HisGlu: 0.492 ± 0.173
1.066HisPhe: 1.066 ± 0.325
0.902HisGly: 0.902 ± 0.255
0.656HisHis: 0.656 ± 0.327
1.23HisIle: 1.23 ± 0.315
0.738HisLys: 0.738 ± 0.282
1.148HisLeu: 1.148 ± 0.319
0.082HisMet: 0.082 ± 0.07
1.066HisAsn: 1.066 ± 0.271
0.574HisPro: 0.574 ± 0.28
0.492HisGln: 0.492 ± 0.177
0.41HisArg: 0.41 ± 0.213
1.148HisSer: 1.148 ± 0.306
0.656HisThr: 0.656 ± 0.168
0.82HisVal: 0.82 ± 0.283
0.0HisTrp: 0.0 ± 0.0
0.574HisTyr: 0.574 ± 0.179
0.0HisXaa: 0.0 ± 0.0
Ile
4.756IleAla: 4.756 ± 0.65
0.984IleCys: 0.984 ± 0.353
4.92IleAsp: 4.92 ± 0.57
7.791IleGlu: 7.791 ± 0.851
3.526IlePhe: 3.526 ± 0.507
3.854IleGly: 3.854 ± 0.736
0.902IleHis: 0.902 ± 0.327
5.084IleIle: 5.084 ± 0.696
8.365IleLys: 8.365 ± 0.98
6.971IleLeu: 6.971 ± 0.912
1.066IleMet: 1.066 ± 0.3
6.315IleAsn: 6.315 ± 0.826
2.542IlePro: 2.542 ± 0.432
2.542IleGln: 2.542 ± 0.601
1.722IleArg: 1.722 ± 0.394
5.33IleSer: 5.33 ± 0.628
4.838IleThr: 4.838 ± 0.876
4.346IleVal: 4.346 ± 0.643
0.902IleTrp: 0.902 ± 0.267
3.198IleTyr: 3.198 ± 0.448
0.0IleXaa: 0.0 ± 0.0
Lys
5.166LysAla: 5.166 ± 0.63
1.312LysCys: 1.312 ± 0.329
4.592LysAsp: 4.592 ± 0.588
9.267LysGlu: 9.267 ± 1.267
3.28LysPhe: 3.28 ± 0.404
4.1LysGly: 4.1 ± 0.528
1.968LysHis: 1.968 ± 0.434
8.037LysIle: 8.037 ± 0.767
7.053LysLys: 7.053 ± 0.861
7.955LysLeu: 7.955 ± 0.855
3.362LysMet: 3.362 ± 0.478
6.233LysAsn: 6.233 ± 0.799
2.542LysPro: 2.542 ± 0.425
4.756LysGln: 4.756 ± 0.76
3.608LysArg: 3.608 ± 0.699
5.248LysSer: 5.248 ± 0.567
6.151LysThr: 6.151 ± 0.685
5.166LysVal: 5.166 ± 0.716
1.23LysTrp: 1.23 ± 0.361
4.1LysTyr: 4.1 ± 0.604
0.0LysXaa: 0.0 ± 0.0
Leu
4.018LeuAla: 4.018 ± 0.777
0.492LeuCys: 0.492 ± 0.217
5.577LeuAsp: 5.577 ± 0.615
6.397LeuGlu: 6.397 ± 1.044
4.018LeuPhe: 4.018 ± 0.476
3.772LeuGly: 3.772 ± 0.453
0.902LeuHis: 0.902 ± 0.283
7.217LeuIle: 7.217 ± 0.781
9.021LeuLys: 9.021 ± 0.858
6.561LeuLeu: 6.561 ± 0.704
1.886LeuMet: 1.886 ± 0.543
7.135LeuAsn: 7.135 ± 0.697
3.69LeuPro: 3.69 ± 0.462
4.346LeuGln: 4.346 ± 0.605
2.952LeuArg: 2.952 ± 0.478
5.412LeuSer: 5.412 ± 0.733
6.151LeuThr: 6.151 ± 0.721
4.51LeuVal: 4.51 ± 0.773
0.82LeuTrp: 0.82 ± 0.282
3.69LeuTyr: 3.69 ± 0.544
0.0LeuXaa: 0.0 ± 0.0
Met
1.722MetAla: 1.722 ± 0.341
0.082MetCys: 0.082 ± 0.087
0.82MetAsp: 0.82 ± 0.273
1.558MetGlu: 1.558 ± 0.358
1.23MetPhe: 1.23 ± 0.278
0.738MetGly: 0.738 ± 0.248
0.082MetHis: 0.082 ± 0.087
1.23MetIle: 1.23 ± 0.362
3.034MetLys: 3.034 ± 0.626
1.804MetLeu: 1.804 ± 0.36
0.328MetMet: 0.328 ± 0.152
1.558MetAsn: 1.558 ± 0.389
0.984MetPro: 0.984 ± 0.318
1.066MetGln: 1.066 ± 0.244
0.82MetArg: 0.82 ± 0.227
1.886MetSer: 1.886 ± 0.369
0.984MetThr: 0.984 ± 0.248
0.902MetVal: 0.902 ± 0.234
0.41MetTrp: 0.41 ± 0.176
0.492MetTyr: 0.492 ± 0.204
0.0MetXaa: 0.0 ± 0.0
Asn
4.51AsnAla: 4.51 ± 0.549
0.656AsnCys: 0.656 ± 0.194
4.756AsnAsp: 4.756 ± 0.7
5.987AsnGlu: 5.987 ± 0.709
4.182AsnPhe: 4.182 ± 0.644
4.182AsnGly: 4.182 ± 0.551
1.148AsnHis: 1.148 ± 0.293
5.33AsnIle: 5.33 ± 0.607
9.185AsnLys: 9.185 ± 1.282
7.053AsnLeu: 7.053 ± 0.991
0.984AsnMet: 0.984 ± 0.38
4.428AsnAsn: 4.428 ± 1.017
2.132AsnPro: 2.132 ± 0.38
2.952AsnGln: 2.952 ± 0.414
2.132AsnArg: 2.132 ± 0.424
5.33AsnSer: 5.33 ± 0.65
4.346AsnThr: 4.346 ± 0.498
5.412AsnVal: 5.412 ± 0.621
0.738AsnTrp: 0.738 ± 0.292
4.51AsnTyr: 4.51 ± 0.525
0.0AsnXaa: 0.0 ± 0.0
Pro
1.476ProAla: 1.476 ± 0.354
0.492ProCys: 0.492 ± 0.176
1.148ProAsp: 1.148 ± 0.253
1.804ProGlu: 1.804 ± 0.411
1.64ProPhe: 1.64 ± 0.372
0.0ProGly: 0.0 ± 0.0
0.082ProHis: 0.082 ± 0.075
1.394ProIle: 1.394 ± 0.31
1.722ProLys: 1.722 ± 0.438
3.034ProLeu: 3.034 ± 0.437
0.82ProMet: 0.82 ± 0.282
2.378ProAsn: 2.378 ± 0.404
0.738ProPro: 0.738 ± 0.303
1.312ProGln: 1.312 ± 0.417
0.41ProArg: 0.41 ± 0.221
2.296ProSer: 2.296 ± 0.47
2.05ProThr: 2.05 ± 0.415
1.312ProVal: 1.312 ± 0.305
0.0ProTrp: 0.0 ± 0.0
1.476ProTyr: 1.476 ± 0.329
0.0ProXaa: 0.0 ± 0.0
Gln
2.05GlnAla: 2.05 ± 0.637
0.328GlnCys: 0.328 ± 0.149
1.312GlnAsp: 1.312 ± 0.29
2.214GlnGlu: 2.214 ± 0.446
1.148GlnPhe: 1.148 ± 0.265
2.132GlnGly: 2.132 ± 0.409
0.738GlnHis: 0.738 ± 0.237
4.346GlnIle: 4.346 ± 0.701
4.018GlnLys: 4.018 ± 0.704
3.772GlnLeu: 3.772 ± 0.507
0.902GlnMet: 0.902 ± 0.339
2.378GlnAsn: 2.378 ± 0.517
1.23GlnPro: 1.23 ± 0.309
1.968GlnGln: 1.968 ± 0.67
1.968GlnArg: 1.968 ± 0.393
2.05GlnSer: 2.05 ± 0.359
2.378GlnThr: 2.378 ± 0.432
2.214GlnVal: 2.214 ± 0.451
0.41GlnTrp: 0.41 ± 0.157
1.558GlnTyr: 1.558 ± 0.349
0.0GlnXaa: 0.0 ± 0.0
Arg
0.902ArgAla: 0.902 ± 0.327
0.492ArgCys: 0.492 ± 0.196
1.476ArgAsp: 1.476 ± 0.324
2.05ArgGlu: 2.05 ± 0.451
1.148ArgPhe: 1.148 ± 0.314
1.066ArgGly: 1.066 ± 0.335
0.492ArgHis: 0.492 ± 0.205
2.788ArgIle: 2.788 ± 0.562
2.624ArgLys: 2.624 ± 0.413
3.034ArgLeu: 3.034 ± 0.407
0.656ArgMet: 0.656 ± 0.203
2.788ArgAsn: 2.788 ± 0.497
0.246ArgPro: 0.246 ± 0.136
0.902ArgGln: 0.902 ± 0.338
0.902ArgArg: 0.902 ± 0.276
2.214ArgSer: 2.214 ± 0.422
2.132ArgThr: 2.132 ± 0.345
2.214ArgVal: 2.214 ± 0.407
0.164ArgTrp: 0.164 ± 0.128
1.886ArgTyr: 1.886 ± 0.398
0.0ArgXaa: 0.0 ± 0.0
Ser
2.132SerAla: 2.132 ± 0.364
0.41SerCys: 0.41 ± 0.185
4.182SerAsp: 4.182 ± 0.714
5.084SerGlu: 5.084 ± 0.653
4.182SerPhe: 4.182 ± 0.684
4.264SerGly: 4.264 ± 0.604
0.82SerHis: 0.82 ± 0.194
5.659SerIle: 5.659 ± 0.644
5.987SerLys: 5.987 ± 0.709
5.823SerLeu: 5.823 ± 0.698
1.23SerMet: 1.23 ± 0.341
5.166SerAsn: 5.166 ± 0.794
1.558SerPro: 1.558 ± 0.367
2.788SerGln: 2.788 ± 0.558
1.886SerArg: 1.886 ± 0.363
3.854SerSer: 3.854 ± 0.712
2.706SerThr: 2.706 ± 0.522
4.756SerVal: 4.756 ± 0.61
0.656SerTrp: 0.656 ± 0.247
1.312SerTyr: 1.312 ± 0.336
0.0SerXaa: 0.0 ± 0.0
Thr
3.936ThrAla: 3.936 ± 0.665
0.574ThrCys: 0.574 ± 0.212
4.756ThrAsp: 4.756 ± 0.497
4.592ThrGlu: 4.592 ± 0.782
4.018ThrPhe: 4.018 ± 0.685
3.362ThrGly: 3.362 ± 0.659
0.984ThrHis: 0.984 ± 0.284
6.315ThrIle: 6.315 ± 0.753
4.92ThrLys: 4.92 ± 0.503
5.823ThrLeu: 5.823 ± 0.735
1.066ThrMet: 1.066 ± 0.335
4.838ThrAsn: 4.838 ± 0.736
2.132ThrPro: 2.132 ± 0.514
3.116ThrGln: 3.116 ± 0.482
1.312ThrArg: 1.312 ± 0.257
3.69ThrSer: 3.69 ± 0.593
5.412ThrThr: 5.412 ± 0.852
1.558ThrVal: 1.558 ± 0.376
0.656ThrTrp: 0.656 ± 0.213
2.296ThrTyr: 2.296 ± 0.421
0.0ThrXaa: 0.0 ± 0.0
Val
2.952ValAla: 2.952 ± 0.488
0.738ValCys: 0.738 ± 0.258
3.28ValAsp: 3.28 ± 0.554
3.116ValGlu: 3.116 ± 0.577
2.706ValPhe: 2.706 ± 0.481
3.198ValGly: 3.198 ± 0.403
0.656ValHis: 0.656 ± 0.219
4.264ValIle: 4.264 ± 0.499
5.248ValLys: 5.248 ± 0.676
4.92ValLeu: 4.92 ± 0.595
1.066ValMet: 1.066 ± 0.258
5.002ValAsn: 5.002 ± 0.824
1.148ValPro: 1.148 ± 0.324
2.132ValGln: 2.132 ± 0.452
1.64ValArg: 1.64 ± 0.441
4.592ValSer: 4.592 ± 0.606
2.542ValThr: 2.542 ± 0.446
3.034ValVal: 3.034 ± 0.564
0.82ValTrp: 0.82 ± 0.241
2.214ValTyr: 2.214 ± 0.324
0.0ValXaa: 0.0 ± 0.0
Trp
0.492TrpAla: 0.492 ± 0.172
0.082TrpCys: 0.082 ± 0.083
0.902TrpAsp: 0.902 ± 0.32
0.738TrpGlu: 0.738 ± 0.235
0.41TrpPhe: 0.41 ± 0.171
0.246TrpGly: 0.246 ± 0.144
0.328TrpHis: 0.328 ± 0.189
0.902TrpIle: 0.902 ± 0.279
0.984TrpLys: 0.984 ± 0.314
1.148TrpLeu: 1.148 ± 0.276
0.082TrpMet: 0.082 ± 0.125
0.82TrpAsn: 0.82 ± 0.264
0.0TrpPro: 0.0 ± 0.0
0.246TrpGln: 0.246 ± 0.139
0.328TrpArg: 0.328 ± 0.153
0.738TrpSer: 0.738 ± 0.282
0.656TrpThr: 0.656 ± 0.228
0.246TrpVal: 0.246 ± 0.159
0.0TrpTrp: 0.0 ± 0.0
0.492TrpTyr: 0.492 ± 0.231
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.64TyrAla: 1.64 ± 0.271
0.574TyrCys: 0.574 ± 0.191
2.952TyrAsp: 2.952 ± 0.509
3.198TyrGlu: 3.198 ± 0.531
2.378TyrPhe: 2.378 ± 0.464
2.05TyrGly: 2.05 ± 0.31
0.738TyrHis: 0.738 ± 0.213
3.28TyrIle: 3.28 ± 0.453
4.674TyrLys: 4.674 ± 0.638
3.854TyrLeu: 3.854 ± 0.465
0.41TyrMet: 0.41 ± 0.188
3.608TyrAsn: 3.608 ± 0.508
1.312TyrPro: 1.312 ± 0.424
1.312TyrGln: 1.312 ± 0.32
1.312TyrArg: 1.312 ± 0.361
2.46TyrSer: 2.46 ± 0.502
2.788TyrThr: 2.788 ± 0.531
2.214TyrVal: 2.214 ± 0.468
0.492TyrTrp: 0.492 ± 0.222
2.214TyrTyr: 2.214 ± 0.444
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (12195 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski