Vulgatibacter incomptus
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3704 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1PCL8|A0A0K1PCL8_9DELT ABC transporter related protein OS=Vulgatibacter incomptus OX=1391653 GN=AKJ08_1251 PE=4 SV=1
MM1 pKa = 7.07 VLSLGGGCSPSVPDD15 pKa = 4.38 GSPAASAGAGGEE27 pKa = 4.39 GGGSGGKK34 pKa = 10.19 GGGSGGVGGGSGGEE48 pKa = 4.09 GGGTGGVGGGTGGVGGGGSTQSPGTHH74 pKa = 6.39 FAATARR80 pKa = 11.84 EE81 pKa = 4.13 WSLPPDD87 pKa = 3.87 SPSPDD92 pKa = 2.87 GFFTFGYY99 pKa = 10.19 YY100 pKa = 10.48 DD101 pKa = 4.0 WTTMDD106 pKa = 4.65 LDD108 pKa = 4.57 GDD110 pKa = 4.29 GKK112 pKa = 10.06 PDD114 pKa = 3.47 LVVYY118 pKa = 9.49 TGASEE123 pKa = 5.38 SNPHH127 pKa = 5.46 WLLYY131 pKa = 10.77 KK132 pKa = 9.86 NTGSGFAATPTEE144 pKa = 4.01 WSLPPDD150 pKa = 3.85 SPHH153 pKa = 7.01 PDD155 pKa = 2.95 GFFTLAYY162 pKa = 10.05 YY163 pKa = 10.72 DD164 pKa = 4.1 WTTMDD169 pKa = 4.65 LDD171 pKa = 4.57 GDD173 pKa = 4.29 GKK175 pKa = 10.12 PDD177 pKa = 3.56 LVVNTGASEE186 pKa = 4.22 TNRR189 pKa = 11.84 RR190 pKa = 11.84 WLDD193 pKa = 3.38 YY194 pKa = 10.9 KK195 pKa = 10.63 NTGTGFAATATEE207 pKa = 4.08 WSLPADD213 pKa = 3.91 SPHH216 pKa = 7.11 PDD218 pKa = 2.93 GFFTFSYY225 pKa = 10.79 YY226 pKa = 11.07 DD227 pKa = 3.63 WTTMDD232 pKa = 4.65 LDD234 pKa = 4.57 GDD236 pKa = 4.29 GKK238 pKa = 10.17 PDD240 pKa = 3.53 LVVNAGASEE249 pKa = 4.24 TNRR252 pKa = 11.84 RR253 pKa = 11.84 WLLYY257 pKa = 10.33 KK258 pKa = 9.93 NTGSGFAATATEE270 pKa = 4.08 WSLPADD276 pKa = 3.66 SPFPGGFFTFSYY288 pKa = 10.83 YY289 pKa = 11.07 DD290 pKa = 3.63 WTTMDD295 pKa = 4.65 LDD297 pKa = 4.57 GDD299 pKa = 4.29 GKK301 pKa = 10.06 PDD303 pKa = 3.47 LVVYY307 pKa = 9.49 TGASEE312 pKa = 5.38 SNPHH316 pKa = 5.46 WLLYY320 pKa = 10.77 KK321 pKa = 9.86 NTGSGFAATATEE333 pKa = 4.2 WSLPPDD339 pKa = 3.76 SPSPNGFFTFSYY351 pKa = 10.77 YY352 pKa = 11.07 DD353 pKa = 3.63 WTTMDD358 pKa = 4.65 LDD360 pKa = 4.57 GDD362 pKa = 4.29 GKK364 pKa = 10.06 PDD366 pKa = 3.47 LVVYY370 pKa = 9.49 TGASEE375 pKa = 5.39 SNPHH379 pKa = 5.13 WLFYY383 pKa = 11.12 KK384 pKa = 9.72 NTGSGFTATATEE396 pKa = 4.13 WTLPADD402 pKa = 4.0 SPHH405 pKa = 7.0 PDD407 pKa = 2.97 GFFTLAYY414 pKa = 10.05 YY415 pKa = 10.72 DD416 pKa = 4.1 WTTMDD421 pKa = 4.65 LDD423 pKa = 4.57 GDD425 pKa = 4.29 GKK427 pKa = 10.12 PDD429 pKa = 3.56 LVVNTGASEE438 pKa = 4.19 TNRR441 pKa = 11.84 RR442 pKa = 11.84 WLLYY446 pKa = 10.79 KK447 pKa = 9.29 NTPP450 pKa = 3.29
Molecular weight: 47.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.923
IPC_protein 3.961
Toseland 3.732
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.113
Wikipedia 3.923
Rodwell 3.783
Grimsley 3.643
Solomon 3.961
Lehninger 3.923
Nozaki 4.075
DTASelect 4.368
Thurlkill 3.783
EMBOSS 3.923
Sillero 4.088
Patrickios 1.405
IPC_peptide 3.961
IPC2_peptide 4.062
IPC2.peptide.svr19 3.965
Protein with the highest isoelectric point:
>tr|A0A0K1PCI4|A0A0K1PCI4_9DELT Acyl carrier protein OS=Vulgatibacter incomptus OX=1391653 GN=acpP PE=3 SV=1
MM1 pKa = 7.44 PVVSGSSVRR10 pKa = 11.84 ASGRR14 pKa = 11.84 GPAASAPGVTASGRR28 pKa = 11.84 VRR30 pKa = 11.84 AASDD34 pKa = 3.06 RR35 pKa = 11.84 RR36 pKa = 11.84 ATATVRR42 pKa = 11.84 DD43 pKa = 4.28 RR44 pKa = 11.84 TASAPGATATGRR56 pKa = 11.84 ARR58 pKa = 11.84 TASAPGATAIGRR70 pKa = 11.84 GLAASGLRR78 pKa = 11.84 ATAIGPALAASVRR91 pKa = 11.84 RR92 pKa = 11.84 ATATGRR98 pKa = 11.84 ARR100 pKa = 11.84 TALARR105 pKa = 11.84 RR106 pKa = 11.84 ATAIGRR112 pKa = 11.84 ARR114 pKa = 11.84 TALARR119 pKa = 11.84 RR120 pKa = 11.84 ATAIGRR126 pKa = 11.84 ARR128 pKa = 11.84 TALARR133 pKa = 11.84 RR134 pKa = 11.84 ATAIGRR140 pKa = 11.84 GPADD144 pKa = 3.09 SDD146 pKa = 3.91 LRR148 pKa = 11.84 VTAIDD153 pKa = 3.54 RR154 pKa = 11.84 ALAASDD160 pKa = 3.87 RR161 pKa = 11.84 PAAAIDD167 pKa = 3.62 RR168 pKa = 11.84 GPAVSDD174 pKa = 3.71 PRR176 pKa = 11.84 ATAIGRR182 pKa = 11.84 ALAASDD188 pKa = 4.39 LRR190 pKa = 11.84 ATAIGRR196 pKa = 11.84 ALAASDD202 pKa = 4.11 LRR204 pKa = 11.84 ASAIGRR210 pKa = 11.84 ARR212 pKa = 11.84 TAWARR217 pKa = 11.84 RR218 pKa = 11.84 ATAIGRR224 pKa = 11.84 ALAASDD230 pKa = 4.39 LRR232 pKa = 11.84 ATAIGRR238 pKa = 11.84 APAALDD244 pKa = 3.68 LRR246 pKa = 11.84 AMAIGRR252 pKa = 11.84 ARR254 pKa = 11.84 TASARR259 pKa = 11.84 RR260 pKa = 11.84 AMAIGLARR268 pKa = 11.84 TASARR273 pKa = 11.84 RR274 pKa = 11.84 AMAIGRR280 pKa = 11.84 ARR282 pKa = 11.84 TASARR287 pKa = 11.84 RR288 pKa = 11.84 AMAIGLARR296 pKa = 11.84 TASARR301 pKa = 11.84 RR302 pKa = 11.84 ATAIGRR308 pKa = 11.84 ARR310 pKa = 11.84 TASARR315 pKa = 11.84 RR316 pKa = 11.84 AMAIGRR322 pKa = 11.84 ARR324 pKa = 11.84 TALARR329 pKa = 11.84 RR330 pKa = 11.84 ATAIGRR336 pKa = 11.84 ARR338 pKa = 11.84 AASAPRR344 pKa = 11.84 ATAIGRR350 pKa = 11.84 APTASAPRR358 pKa = 11.84 AMAIGRR364 pKa = 11.84 ARR366 pKa = 11.84 TASARR371 pKa = 11.84 RR372 pKa = 11.84 AMAIGRR378 pKa = 11.84 ARR380 pKa = 11.84 TALVRR385 pKa = 11.84 RR386 pKa = 11.84 ATAIGRR392 pKa = 11.84 ARR394 pKa = 11.84 AASVRR399 pKa = 11.84 RR400 pKa = 11.84 AMAIGRR406 pKa = 11.84 ARR408 pKa = 11.84 AASVRR413 pKa = 11.84 RR414 pKa = 11.84 AMAIGRR420 pKa = 11.84 ARR422 pKa = 11.84 ATSARR427 pKa = 11.84 RR428 pKa = 11.84 AMAIVRR434 pKa = 11.84 ARR436 pKa = 11.84 TASARR441 pKa = 11.84 RR442 pKa = 11.84 ATAIGRR448 pKa = 11.84 ARR450 pKa = 11.84 TASAPDD456 pKa = 3.42 ATATAPIAASIGRR469 pKa = 11.84 LVPARR474 pKa = 11.84 PAMPRR479 pKa = 11.84 GGLRR483 pKa = 11.84 AAPIGVRR490 pKa = 11.84 RR491 pKa = 11.84 PGAAEE496 pKa = 3.64 ATIGRR501 pKa = 11.84 VAA503 pKa = 3.74
Molecular weight: 50.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.457
IPC2_protein 10.994
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.247
Rodwell 12.266
Grimsley 12.808
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 11.974
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.162
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3704
0
3704
1270377
37
3785
343.0
37.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.517 ± 0.066
1.036 ± 0.029
5.584 ± 0.032
6.777 ± 0.046
3.486 ± 0.023
9.322 ± 0.056
1.884 ± 0.018
4.02 ± 0.029
2.998 ± 0.039
10.398 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.761 ± 0.019
1.99 ± 0.024
5.714 ± 0.033
2.607 ± 0.022
8.173 ± 0.05
5.927 ± 0.042
4.831 ± 0.056
7.753 ± 0.037
1.32 ± 0.018
1.902 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here