Amino acid dipepetide frequency for Lachnospiraceae bacterium KHCPX20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.354AlaAla: 6.354 ± 0.107	0.916AlaCys: 0.916 ± 0.033	4.589AlaAsp: 4.589 ± 0.075	4.906AlaGlu: 4.906 ± 0.091	2.959AlaPhe: 2.959 ± 0.054	5.635AlaGly: 5.635 ± 0.094	1.079AlaHis: 1.079 ± 0.036	5.098AlaIle: 5.098 ± 0.079	5.827AlaLys: 5.827 ± 0.091	6.904AlaLeu: 6.904 ± 0.093	2.543AlaMet: 2.543 ± 0.059	2.868AlaAsn: 2.868 ± 0.068	1.977AlaPro: 1.977 ± 0.051	2.199AlaGln: 2.199 ± 0.057	3.099AlaArg: 3.099 ± 0.061	4.522AlaSer: 4.522 ± 0.084	4.106AlaThr: 4.106 ± 0.093	5.55AlaVal: 5.55 ± 0.09	0.544AlaTrp: 0.544 ± 0.024	2.753AlaTyr: 2.753 ± 0.06	0.001AlaXaa: 0.001 ± 0.001
Cys	0.777CysAla: 0.777 ± 0.031	0.222CysCys: 0.222 ± 0.016	0.902CysAsp: 0.902 ± 0.032	0.801CysGlu: 0.801 ± 0.03	0.608CysPhe: 0.608 ± 0.029	1.308CysGly: 1.308 ± 0.043	0.319CysHis: 0.319 ± 0.019	0.991CysIle: 0.991 ± 0.031	0.8CysLys: 0.8 ± 0.03	1.013CysLeu: 1.013 ± 0.032	0.357CysMet: 0.357 ± 0.018	0.546CysAsn: 0.546 ± 0.027	0.544CysPro: 0.544 ± 0.025	0.417CysGln: 0.417 ± 0.021	0.658CysArg: 0.658 ± 0.027	0.792CysSer: 0.792 ± 0.031	0.532CysThr: 0.532 ± 0.025	0.931CysVal: 0.931 ± 0.031	0.095CysTrp: 0.095 ± 0.011	0.557CysTyr: 0.557 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	4.865AspAla: 4.865 ± 0.077	0.778AspCys: 0.778 ± 0.033	3.465AspAsp: 3.465 ± 0.076	5.037AspGlu: 5.037 ± 0.067	2.856AspPhe: 2.856 ± 0.061	4.532AspGly: 4.532 ± 0.089	1.257AspHis: 1.257 ± 0.046	4.382AspIle: 4.382 ± 0.07	3.784AspLys: 3.784 ± 0.068	5.263AspLeu: 5.263 ± 0.069	1.842AspMet: 1.842 ± 0.046	2.279AspAsn: 2.279 ± 0.047	2.316AspPro: 2.316 ± 0.053	1.887AspGln: 1.887 ± 0.051	2.764AspArg: 2.764 ± 0.055	3.218AspSer: 3.218 ± 0.061	3.186AspThr: 3.186 ± 0.063	4.364AspVal: 4.364 ± 0.079	0.627AspTrp: 0.627 ± 0.027	3.148AspTyr: 3.148 ± 0.062	0.0AspXaa: 0.0 ± 0.0
Glu	5.609GluAla: 5.609 ± 0.097	0.751GluCys: 0.751 ± 0.028	5.11GluAsp: 5.11 ± 0.086	7.608GluGlu: 7.608 ± 0.137	2.328GluPhe: 2.328 ± 0.047	4.356GluGly: 4.356 ± 0.073	1.455GluHis: 1.455 ± 0.039	5.306GluIle: 5.306 ± 0.083	6.66GluLys: 6.66 ± 0.107	6.354GluLeu: 6.354 ± 0.096	2.39GluMet: 2.39 ± 0.055	3.773GluAsn: 3.773 ± 0.069	1.685GluPro: 1.685 ± 0.049	2.902GluGln: 2.902 ± 0.061	3.292GluArg: 3.292 ± 0.07	3.345GluSer: 3.345 ± 0.073	3.219GluThr: 3.219 ± 0.063	4.439GluVal: 4.439 ± 0.08	0.605GluTrp: 0.605 ± 0.026	2.978GluTyr: 2.978 ± 0.058	0.0GluXaa: 0.0 ± 0.0
Phe	2.942PheAla: 2.942 ± 0.059	0.644PheCys: 0.644 ± 0.027	2.709PheAsp: 2.709 ± 0.053	2.477PheGlu: 2.477 ± 0.06	1.87PhePhe: 1.87 ± 0.054	2.751PheGly: 2.751 ± 0.062	0.948PheHis: 0.948 ± 0.031	2.437PheIle: 2.437 ± 0.057	2.003PheLys: 2.003 ± 0.043	3.756PheLeu: 3.756 ± 0.08	1.115PheMet: 1.115 ± 0.034	1.498PheAsn: 1.498 ± 0.043	1.286PhePro: 1.286 ± 0.038	1.14PheGln: 1.14 ± 0.036	1.689PheArg: 1.689 ± 0.042	2.847PheSer: 2.847 ± 0.056	2.377PheThr: 2.377 ± 0.055	2.755PheVal: 2.755 ± 0.056	0.397PheTrp: 0.397 ± 0.023	1.757PheTyr: 1.757 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	4.708GlyAla: 4.708 ± 0.092	1.08GlyCys: 1.08 ± 0.037	3.773GlyAsp: 3.773 ± 0.079	4.685GlyGlu: 4.685 ± 0.087	2.951GlyPhe: 2.951 ± 0.058	4.612GlyGly: 4.612 ± 0.096	1.406GlyHis: 1.406 ± 0.039	5.73GlyIle: 5.73 ± 0.077	5.554GlyLys: 5.554 ± 0.084	5.408GlyLeu: 5.408 ± 0.087	2.286GlyMet: 2.286 ± 0.051	3.03GlyAsn: 3.03 ± 0.058	1.317GlyPro: 1.317 ± 0.041	2.153GlyGln: 2.153 ± 0.051	3.138GlyArg: 3.138 ± 0.059	4.18GlySer: 4.18 ± 0.069	3.908GlyThr: 3.908 ± 0.074	4.685GlyVal: 4.685 ± 0.079	0.638GlyTrp: 0.638 ± 0.029	3.291GlyTyr: 3.291 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	1.209HisAla: 1.209 ± 0.037	0.297HisCys: 0.297 ± 0.019	1.029HisAsp: 1.029 ± 0.039	1.134HisGlu: 1.134 ± 0.035	0.835HisPhe: 0.835 ± 0.03	1.334HisGly: 1.334 ± 0.04	0.456HisHis: 0.456 ± 0.028	1.33HisIle: 1.33 ± 0.038	1.149HisLys: 1.149 ± 0.034	1.662HisLeu: 1.662 ± 0.043	0.593HisMet: 0.593 ± 0.027	0.79HisAsn: 0.79 ± 0.03	0.973HisPro: 0.973 ± 0.034	0.653HisGln: 0.653 ± 0.026	0.938HisArg: 0.938 ± 0.034	1.022HisSer: 1.022 ± 0.036	0.998HisThr: 0.998 ± 0.031	1.291HisVal: 1.291 ± 0.034	0.168HisTrp: 0.168 ± 0.013	0.868HisTyr: 0.868 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.569IleAla: 5.569 ± 0.087	1.118IleCys: 1.118 ± 0.032	4.204IleAsp: 4.204 ± 0.07	4.434IleGlu: 4.434 ± 0.071	2.719IlePhe: 2.719 ± 0.062	4.719IleGly: 4.719 ± 0.088	1.428IleHis: 1.428 ± 0.042	4.392IleIle: 4.392 ± 0.072	4.315IleLys: 4.315 ± 0.076	6.552IleLeu: 6.552 ± 0.096	1.821IleMet: 1.821 ± 0.053	2.77IleAsn: 2.77 ± 0.058	2.994IlePro: 2.994 ± 0.058	2.197IleGln: 2.197 ± 0.053	3.829IleArg: 3.829 ± 0.059	4.733IleSer: 4.733 ± 0.071	4.328IleThr: 4.328 ± 0.083	4.503IleVal: 4.503 ± 0.078	0.519IleTrp: 0.519 ± 0.026	2.662IleTyr: 2.662 ± 0.056	0.0IleXaa: 0.0 ± 0.0
Lys	5.677LysAla: 5.677 ± 0.099	0.581LysCys: 0.581 ± 0.026	4.991LysAsp: 4.991 ± 0.08	7.211LysGlu: 7.211 ± 0.108	1.751LysPhe: 1.751 ± 0.047	4.651LysGly: 4.651 ± 0.069	1.064LysHis: 1.064 ± 0.036	4.673LysIle: 4.673 ± 0.078	7.332LysLys: 7.332 ± 0.119	5.324LysLeu: 5.324 ± 0.074	2.122LysMet: 2.122 ± 0.045	3.843LysAsn: 3.843 ± 0.077	1.95LysPro: 1.95 ± 0.047	2.36LysGln: 2.36 ± 0.06	3.37LysArg: 3.37 ± 0.07	3.739LysSer: 3.739 ± 0.071	4.156LysThr: 4.156 ± 0.077	4.846LysVal: 4.846 ± 0.086	0.56LysTrp: 0.56 ± 0.028	2.821LysTyr: 2.821 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	6.505LeuAla: 6.505 ± 0.084	1.435LeuCys: 1.435 ± 0.041	5.233LeuAsp: 5.233 ± 0.087	5.613LeuGlu: 5.613 ± 0.091	3.47LeuPhe: 3.47 ± 0.084	5.494LeuGly: 5.494 ± 0.088	1.713LeuHis: 1.713 ± 0.055	5.511LeuIle: 5.511 ± 0.091	6.054LeuLys: 6.054 ± 0.092	8.351LeuLeu: 8.351 ± 0.139	2.665LeuMet: 2.665 ± 0.058	3.654LeuAsn: 3.654 ± 0.066	3.436LeuPro: 3.436 ± 0.07	2.9LeuGln: 2.9 ± 0.051	3.924LeuArg: 3.924 ± 0.072	7.092LeuSer: 7.092 ± 0.097	5.273LeuThr: 5.273 ± 0.082	5.321LeuVal: 5.321 ± 0.089	0.695LeuTrp: 0.695 ± 0.031	3.337LeuTyr: 3.337 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	2.417MetAla: 2.417 ± 0.047	0.307MetCys: 0.307 ± 0.019	2.201MetAsp: 2.201 ± 0.052	2.427MetGlu: 2.427 ± 0.051	0.983MetPhe: 0.983 ± 0.039	1.913MetGly: 1.913 ± 0.051	0.502MetHis: 0.502 ± 0.024	2.146MetIle: 2.146 ± 0.055	2.427MetLys: 2.427 ± 0.049	2.603MetLeu: 2.603 ± 0.061	1.034MetMet: 1.034 ± 0.033	1.563MetAsn: 1.563 ± 0.037	1.063MetPro: 1.063 ± 0.034	1.09MetGln: 1.09 ± 0.035	1.229MetArg: 1.229 ± 0.039	1.831MetSer: 1.831 ± 0.045	1.823MetThr: 1.823 ± 0.046	2.015MetVal: 2.015 ± 0.044	0.163MetTrp: 0.163 ± 0.012	0.923MetTyr: 0.923 ± 0.041	0.0MetXaa: 0.0 ± 0.0
Asn	3.292AsnAla: 3.292 ± 0.068	0.6AsnCys: 0.6 ± 0.029	2.361AsnAsp: 2.361 ± 0.061	2.803AsnGlu: 2.803 ± 0.062	1.616AsnPhe: 1.616 ± 0.041	3.574AsnGly: 3.574 ± 0.068	0.931AsnHis: 0.931 ± 0.032	3.084AsnIle: 3.084 ± 0.062	2.795AsnLys: 2.795 ± 0.062	3.861AsnLeu: 3.861 ± 0.073	1.313AsnMet: 1.313 ± 0.038	1.942AsnAsn: 1.942 ± 0.062	1.885AsnPro: 1.885 ± 0.047	1.482AsnGln: 1.482 ± 0.039	2.191AsnArg: 2.191 ± 0.053	2.274AsnSer: 2.274 ± 0.053	2.485AsnThr: 2.485 ± 0.063	2.896AsnVal: 2.896 ± 0.07	0.403AsnTrp: 0.403 ± 0.019	1.854AsnTyr: 1.854 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	2.237ProAla: 2.237 ± 0.059	0.378ProCys: 0.378 ± 0.021	2.133ProAsp: 2.133 ± 0.048	2.881ProGlu: 2.881 ± 0.063	1.414ProPhe: 1.414 ± 0.034	2.17ProGly: 2.17 ± 0.047	0.513ProHis: 0.513 ± 0.025	1.997ProIle: 1.997 ± 0.042	2.353ProLys: 2.353 ± 0.052	2.685ProLeu: 2.685 ± 0.06	0.927ProMet: 0.927 ± 0.035	1.213ProAsn: 1.213 ± 0.036	0.662ProPro: 0.662 ± 0.031	1.142ProGln: 1.142 ± 0.033	1.011ProArg: 1.011 ± 0.035	1.984ProSer: 1.984 ± 0.049	1.607ProThr: 1.607 ± 0.043	2.826ProVal: 2.826 ± 0.062	0.316ProTrp: 0.316 ± 0.019	1.458ProTyr: 1.458 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	2.407GlnAla: 2.407 ± 0.053	0.313GlnCys: 0.313 ± 0.019	1.657GlnAsp: 1.657 ± 0.044	2.685GlnGlu: 2.685 ± 0.058	1.114GlnPhe: 1.114 ± 0.033	1.807GlnGly: 1.807 ± 0.044	0.524GlnHis: 0.524 ± 0.024	2.518GlnIle: 2.518 ± 0.053	2.957GlnLys: 2.957 ± 0.059	2.914GlnLeu: 2.914 ± 0.066	1.228GlnMet: 1.228 ± 0.037	1.495GlnAsn: 1.495 ± 0.045	0.87GlnPro: 0.87 ± 0.028	1.372GlnGln: 1.372 ± 0.038	1.613GlnArg: 1.613 ± 0.049	1.742GlnSer: 1.742 ± 0.042	1.698GlnThr: 1.698 ± 0.042	2.129GlnVal: 2.129 ± 0.051	0.315GlnTrp: 0.315 ± 0.016	1.342GlnTyr: 1.342 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.785ArgAla: 2.785 ± 0.049	0.553ArgCys: 0.553 ± 0.021	2.579ArgAsp: 2.579 ± 0.049	3.719ArgGlu: 3.719 ± 0.073	1.89ArgPhe: 1.89 ± 0.049	2.573ArgGly: 2.573 ± 0.064	0.903ArgHis: 0.903 ± 0.037	3.697ArgIle: 3.697 ± 0.071	3.619ArgLys: 3.619 ± 0.073	4.054ArgLeu: 4.054 ± 0.071	1.646ArgMet: 1.646 ± 0.047	2.156ArgAsn: 2.156 ± 0.045	1.328ArgPro: 1.328 ± 0.036	1.833ArgGln: 1.833 ± 0.046	2.346ArgArg: 2.346 ± 0.059	2.439ArgSer: 2.439 ± 0.062	2.212ArgThr: 2.212 ± 0.053	2.731ArgVal: 2.731 ± 0.058	0.335ArgTrp: 0.335 ± 0.02	1.954ArgTyr: 1.954 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	4.291SerAla: 4.291 ± 0.07	0.777SerCys: 0.777 ± 0.032	3.929SerAsp: 3.929 ± 0.072	3.921SerGlu: 3.921 ± 0.063	2.85SerPhe: 2.85 ± 0.063	4.88SerGly: 4.88 ± 0.084	1.11SerHis: 1.11 ± 0.033	4.226SerIle: 4.226 ± 0.073	3.867SerLys: 3.867 ± 0.068	5.469SerLeu: 5.469 ± 0.093	1.882SerMet: 1.882 ± 0.042	2.613SerAsn: 2.613 ± 0.072	1.689SerPro: 1.689 ± 0.045	1.753SerGln: 1.753 ± 0.043	2.708SerArg: 2.708 ± 0.056	4.062SerSer: 4.062 ± 0.083	2.987SerThr: 2.987 ± 0.068	4.477SerVal: 4.477 ± 0.069	0.539SerTrp: 0.539 ± 0.027	2.779SerTyr: 2.779 ± 0.068	0.0SerXaa: 0.0 ± 0.0
Thr	4.306ThrAla: 4.306 ± 0.094	0.609ThrCys: 0.609 ± 0.025	3.358ThrAsp: 3.358 ± 0.062	3.747ThrGlu: 3.747 ± 0.064	2.314ThrPhe: 2.314 ± 0.055	4.566ThrGly: 4.566 ± 0.079	0.85ThrHis: 0.85 ± 0.031	4.098ThrIle: 4.098 ± 0.073	3.568ThrLys: 3.568 ± 0.073	4.836ThrLeu: 4.836 ± 0.074	1.459ThrMet: 1.459 ± 0.042	2.202ThrAsn: 2.202 ± 0.056	2.063ThrPro: 2.063 ± 0.044	1.531ThrGln: 1.531 ± 0.045	2.132ThrArg: 2.132 ± 0.048	3.297ThrSer: 3.297 ± 0.069	3.234ThrThr: 3.234 ± 0.078	4.504ThrVal: 4.504 ± 0.092	0.503ThrTrp: 0.503 ± 0.024	2.376ThrTyr: 2.376 ± 0.059	0.0ThrXaa: 0.0 ± 0.0
Val	5.272ValAla: 5.272 ± 0.095	1.049ValCys: 1.049 ± 0.038	4.246ValAsp: 4.246 ± 0.067	4.468ValGlu: 4.468 ± 0.078	2.754ValPhe: 2.754 ± 0.058	4.17ValGly: 4.17 ± 0.072	1.102ValHis: 1.102 ± 0.036	4.843ValIle: 4.843 ± 0.078	4.758ValLys: 4.758 ± 0.078	6.287ValLeu: 6.287 ± 0.093	2.039ValMet: 2.039 ± 0.054	2.947ValAsn: 2.947 ± 0.053	2.247ValPro: 2.247 ± 0.058	1.655ValGln: 1.655 ± 0.038	2.888ValArg: 2.888 ± 0.059	4.786ValSer: 4.786 ± 0.074	4.639ValThr: 4.639 ± 0.104	4.896ValVal: 4.896 ± 0.086	0.555ValTrp: 0.555 ± 0.022	2.661ValTyr: 2.661 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.502TrpAla: 0.502 ± 0.023	0.143TrpCys: 0.143 ± 0.012	0.562TrpAsp: 0.562 ± 0.024	0.634TrpGlu: 0.634 ± 0.029	0.36TrpPhe: 0.36 ± 0.022	0.534TrpGly: 0.534 ± 0.029	0.173TrpHis: 0.173 ± 0.015	0.624TrpIle: 0.624 ± 0.028	0.647TrpLys: 0.647 ± 0.029	0.724TrpLeu: 0.724 ± 0.032	0.302TrpMet: 0.302 ± 0.018	0.543TrpAsn: 0.543 ± 0.025	0.177TrpPro: 0.177 ± 0.012	0.367TrpGln: 0.367 ± 0.021	0.317TrpArg: 0.317 ± 0.021	0.471TrpSer: 0.471 ± 0.024	0.399TrpThr: 0.399 ± 0.023	0.462TrpVal: 0.462 ± 0.021	0.116TrpTrp: 0.116 ± 0.011	0.372TrpTyr: 0.372 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.704TyrAla: 2.704 ± 0.061	0.622TyrCys: 0.622 ± 0.031	2.819TyrAsp: 2.819 ± 0.061	2.978TyrGlu: 2.978 ± 0.058	1.756TyrPhe: 1.756 ± 0.046	2.954TyrGly: 2.954 ± 0.066	0.994TyrHis: 0.994 ± 0.033	2.722TyrIle: 2.722 ± 0.057	2.554TyrLys: 2.554 ± 0.057	3.654TyrLeu: 3.654 ± 0.074	1.095TyrMet: 1.095 ± 0.04	1.916TyrAsn: 1.916 ± 0.046	1.443TyrPro: 1.443 ± 0.039	1.667TyrGln: 1.667 ± 0.044	2.216TyrArg: 2.216 ± 0.055	2.432TyrSer: 2.432 ± 0.057	2.388TyrThr: 2.388 ± 0.067	2.641TyrVal: 2.641 ± 0.051	0.337TyrTrp: 0.337 ± 0.019	2.025TyrTyr: 2.025 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 2926 proteins (953569 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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