Ochrobactrum thiophenivorans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Brucellaceae; Brucella/Ochrobactrum group; Brucella

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4365 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A256FZM1|A0A256FZM1_9RHIZ 50S ribosomal protein L9 OS=Ochrobactrum thiophenivorans OX=571255 GN=rplI PE=3 SV=1
MM1 pKa = 7.43KK2 pKa = 10.22KK3 pKa = 9.41WILVAGLSLIASGAFAGEE21 pKa = 3.98ITIKK25 pKa = 10.77LADD28 pKa = 4.2DD29 pKa = 3.68VDD31 pKa = 4.05VTEE34 pKa = 4.2NAVLYY39 pKa = 10.68KK40 pKa = 10.86CGDD43 pKa = 3.67QNVSATYY50 pKa = 10.94YY51 pKa = 10.36NAGDD55 pKa = 3.54NSLVEE60 pKa = 5.54LEE62 pKa = 4.52MEE64 pKa = 4.4NNTVIAANVLSGSGAKK80 pKa = 9.51YY81 pKa = 10.44AGGIYY86 pKa = 9.81VWWTKK91 pKa = 10.79GPTADD96 pKa = 5.15LYY98 pKa = 11.91NLMDD102 pKa = 5.14DD103 pKa = 4.71PEE105 pKa = 4.2QEE107 pKa = 4.3KK108 pKa = 10.47PISCTEE114 pKa = 3.84EE115 pKa = 3.7

Molecular weight: 12.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A256G4L7|A0A256G4L7_9RHIZ Transcriptional activator HlyU family protein OS=Ochrobactrum thiophenivorans OX=571255 GN=CEV31_0392 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAAGRR28 pKa = 11.84KK29 pKa = 8.62VLAARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.96RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4365

0

4365

1258635

35

2865

288.3

31.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.891 ± 0.047

0.788 ± 0.011

5.603 ± 0.03

5.827 ± 0.036

4.064 ± 0.025

8.04 ± 0.036

2.018 ± 0.019

6.299 ± 0.031

4.351 ± 0.031

9.912 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.019

3.361 ± 0.022

4.593 ± 0.026

3.277 ± 0.025

6.096 ± 0.037

6.061 ± 0.028

5.329 ± 0.024

7.166 ± 0.034

1.249 ± 0.017

2.459 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski