Brachybacterium endophyticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Dermabacteraceae; Brachybacterium

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3043 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U2RIW5|A0A2U2RIW5_9MICO Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase OS=Brachybacterium endophyticum OX=2182385 GN=lgt PE=3 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84RR3 pKa = 11.84CLHH6 pKa = 6.23LAGGGLFAAGLVLGSASMASAGPDD30 pKa = 3.34DD31 pKa = 5.5DD32 pKa = 4.41VWEE35 pKa = 4.74GDD37 pKa = 3.99SVQKK41 pKa = 10.36PVSEE45 pKa = 3.93QKK47 pKa = 10.48AAQEE51 pKa = 3.99YY52 pKa = 5.73WTPEE56 pKa = 3.41RR57 pKa = 11.84MEE59 pKa = 4.16NAKK62 pKa = 10.65SADD65 pKa = 3.55NLVSDD70 pKa = 4.78KK71 pKa = 10.73DD72 pKa = 3.84APSGDD77 pKa = 3.83VKK79 pKa = 11.15SSDD82 pKa = 3.46PSEE85 pKa = 4.38VPSQKK90 pKa = 10.6ADD92 pKa = 3.52PAVQTRR98 pKa = 11.84SATSEE103 pKa = 3.75KK104 pKa = 9.43TAAGTDD110 pKa = 3.63HH111 pKa = 6.81IGKK114 pKa = 9.18VFFTQGGSDD123 pKa = 3.88YY124 pKa = 10.48VCSGNAVSSGNKK136 pKa = 8.13STVATAGHH144 pKa = 6.76CANEE148 pKa = 4.32GGTWATNWTFAPGYY162 pKa = 10.09DD163 pKa = 4.79HH164 pKa = 7.67GDD166 pKa = 3.29TPYY169 pKa = 11.48GLWTASDD176 pKa = 4.7LYY178 pKa = 10.67STQQWISSEE187 pKa = 3.76DD188 pKa = 3.52MNYY191 pKa = 10.58DD192 pKa = 2.86AAFAVVQPEE201 pKa = 4.4SGSDD205 pKa = 3.24TLTDD209 pKa = 3.56AVGGSALSFNGPRR222 pKa = 11.84GEE224 pKa = 4.17QEE226 pKa = 3.58TSYY229 pKa = 10.5GYY231 pKa = 9.41PAAAPFDD238 pKa = 4.16GEE240 pKa = 4.65SLEE243 pKa = 4.35NCQATATDD251 pKa = 4.07DD252 pKa = 4.02TVGDD256 pKa = 4.07TDD258 pKa = 6.05DD259 pKa = 5.18LAIDD263 pKa = 4.06CDD265 pKa = 3.89MTGGSSGGPWFLGSDD280 pKa = 3.34ASGSQNSVNSFGYY293 pKa = 6.12TTQPNVMYY301 pKa = 10.22GPYY304 pKa = 9.91FGDD307 pKa = 3.91VIKK310 pKa = 10.07STYY313 pKa = 10.3DD314 pKa = 3.16QASKK318 pKa = 11.07SS319 pKa = 3.6

Molecular weight: 33.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U2RPI6|A0A2U2RPI6_9MICO DNA-directed RNA polymerase subunit beta' OS=Brachybacterium endophyticum OX=2182385 GN=rpoC PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.44LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84HH26 pKa = 3.6QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3043

0

3043

1043044

27

3522

342.8

36.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.676 ± 0.055

0.562 ± 0.01

6.424 ± 0.044

6.257 ± 0.046

2.811 ± 0.025

9.303 ± 0.043

2.241 ± 0.023

4.118 ± 0.029

1.864 ± 0.033

10.262 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.937 ± 0.019

1.638 ± 0.021

5.66 ± 0.035

2.924 ± 0.022

7.722 ± 0.051

6.025 ± 0.034

5.975 ± 0.026

8.397 ± 0.038

1.407 ± 0.019

1.796 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski