Synechococcus phage S-B28
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A482IFP0|A0A482IFP0_9CAUD Core protein OS=Synechococcus phage S-B28 OX=2545435 PE=4 SV=1
MM1 pKa = 7.32 TATLSKK7 pKa = 10.29 LTGQDD12 pKa = 3.71 LIDD15 pKa = 3.89 HH16 pKa = 6.24 VKK18 pKa = 10.5 LHH20 pKa = 6.79 NDD22 pKa = 3.48 CCSKK26 pKa = 11.24 SEE28 pKa = 3.89 MCLDD32 pKa = 3.44 AGYY35 pKa = 10.93 VNKK38 pKa = 10.67 NGGPAFTDD46 pKa = 5.05 FYY48 pKa = 10.98 TALLEE53 pKa = 4.87 AKK55 pKa = 9.35 TDD57 pKa = 3.97 LNQMPEE63 pKa = 4.1 EE64 pKa = 4.4 SQSGADD70 pKa = 3.03 WYY72 pKa = 10.75 DD73 pKa = 3.37 NLSEE77 pKa = 4.18 QDD79 pKa = 3.42 QLLYY83 pKa = 10.95 DD84 pKa = 4.41 QIEE87 pKa = 4.39 DD88 pKa = 3.69 MCPEE92 pKa = 4.32 FAKK95 pKa = 9.69 LTAEE99 pKa = 4.14 EE100 pKa = 4.44 CQEE103 pKa = 4.3 FMDD106 pKa = 4.42 EE107 pKa = 4.8 LSDD110 pKa = 4.33 IGITTAQQFEE120 pKa = 4.45 DD121 pKa = 4.05 AYY123 pKa = 11.47 YY124 pKa = 9.38 MQFDD128 pKa = 3.78 SYY130 pKa = 10.87 KK131 pKa = 10.45 AEE133 pKa = 3.85 RR134 pKa = 11.84 EE135 pKa = 3.93 FAEE138 pKa = 4.48 EE139 pKa = 4.53 LMCQVTPNLEE149 pKa = 4.15 DD150 pKa = 3.43 SPVFFAIDD158 pKa = 3.33 WQRR161 pKa = 11.84 VWDD164 pKa = 4.2 HH165 pKa = 5.98 SLSYY169 pKa = 10.96 DD170 pKa = 3.72 FSTIEE175 pKa = 4.37 FDD177 pKa = 2.95 GTTYY181 pKa = 10.49 FFHH184 pKa = 7.21 NNN186 pKa = 2.92
Molecular weight: 21.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.794
IPC2_protein 3.834
IPC_protein 3.821
Toseland 3.617
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.643
Grimsley 3.516
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.656
EMBOSS 3.732
Sillero 3.935
Patrickios 0.451
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.839
Protein with the highest isoelectric point:
>tr|A0A482IFQ7|A0A482IFQ7_9CAUD Uncharacterized protein OS=Synechococcus phage S-B28 OX=2545435 PE=4 SV=1
MM1 pKa = 7.83 AKK3 pKa = 10.22 PIRR6 pKa = 11.84 NSLGPLLLHH15 pKa = 6.7 HH16 pKa = 6.68 GCDD19 pKa = 3.07 RR20 pKa = 11.84 SLANLVLEE28 pKa = 5.34 GKK30 pKa = 8.59 TSPIPSEE37 pKa = 4.55 NKK39 pKa = 10.04 YY40 pKa = 10.86 DD41 pKa = 3.46 WLGPGIYY48 pKa = 9.7 FWVDD52 pKa = 3.04 SPSRR56 pKa = 11.84 AMTWARR62 pKa = 11.84 EE63 pKa = 4.0 KK64 pKa = 11.14 YY65 pKa = 10.02 NRR67 pKa = 11.84 EE68 pKa = 3.68 TGSNLKK74 pKa = 10.46 APQPKK79 pKa = 9.79 GGPRR83 pKa = 11.84 KK84 pKa = 9.73 RR85 pKa = 11.84 SFCARR90 pKa = 11.84 MSGVKK95 pKa = 10.52 GPMKK99 pKa = 10.2 DD100 pKa = 3.05 EE101 pKa = 4.46 KK102 pKa = 11.11 GRR104 pKa = 11.84 PTRR107 pKa = 11.84 KK108 pKa = 9.9 ALALRR113 pKa = 11.84 KK114 pKa = 8.38 WKK116 pKa = 10.63 CC117 pKa = 3.1
Molecular weight: 13.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.261
IPC2_protein 9.589
IPC_protein 9.765
Toseland 10.628
ProMoST 10.145
Dawson 10.716
Bjellqvist 10.335
Wikipedia 10.833
Rodwell 11.213
Grimsley 10.745
Solomon 10.76
Lehninger 10.745
Nozaki 10.613
DTASelect 10.321
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.628
Patrickios 10.95
IPC_peptide 10.774
IPC2_peptide 9.107
IPC2.peptide.svr19 8.592
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
14035
40
1356
255.2
28.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.351 ± 0.421
0.955 ± 0.197
6.006 ± 0.263
6.398 ± 0.403
3.591 ± 0.178
6.975 ± 0.38
1.482 ± 0.2
5.33 ± 0.179
5.657 ± 0.383
8.272 ± 0.294
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.508 ± 0.174
5.33 ± 0.232
4.339 ± 0.195
4.973 ± 0.412
4.966 ± 0.261
6.79 ± 0.326
6.74 ± 0.492
6.227 ± 0.337
1.247 ± 0.124
3.862 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here