Amino acid dipepetide frequency for Synechococcus phage S-B28

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.263AlaAla: 9.263 ± 1.03
0.926AlaCys: 0.926 ± 0.271
3.848AlaAsp: 3.848 ± 0.502
4.56AlaGlu: 4.56 ± 0.71
3.349AlaPhe: 3.349 ± 0.511
6.128AlaGly: 6.128 ± 0.726
0.784AlaHis: 0.784 ± 0.246
3.563AlaIle: 3.563 ± 0.392
4.56AlaLys: 4.56 ± 0.69
7.126AlaLeu: 7.126 ± 0.693
2.494AlaMet: 2.494 ± 0.417
4.56AlaAsn: 4.56 ± 0.53
3.777AlaPro: 3.777 ± 0.503
4.917AlaGln: 4.917 ± 0.955
3.99AlaArg: 3.99 ± 0.584
6.199AlaSer: 6.199 ± 0.725
5.487AlaThr: 5.487 ± 0.772
4.774AlaVal: 4.774 ± 0.577
0.641AlaTrp: 0.641 ± 0.235
2.565AlaTyr: 2.565 ± 0.345
0.0AlaXaa: 0.0 ± 0.0
Cys
0.57CysAla: 0.57 ± 0.235
0.285CysCys: 0.285 ± 0.152
0.926CysAsp: 0.926 ± 0.283
0.285CysGlu: 0.285 ± 0.133
0.214CysPhe: 0.214 ± 0.126
0.641CysGly: 0.641 ± 0.221
0.214CysHis: 0.214 ± 0.115
0.214CysIle: 0.214 ± 0.111
1.14CysLys: 1.14 ± 0.385
0.57CysLeu: 0.57 ± 0.23
0.285CysMet: 0.285 ± 0.14
0.428CysAsn: 0.428 ± 0.156
0.143CysPro: 0.143 ± 0.097
0.641CysGln: 0.641 ± 0.201
0.285CysArg: 0.285 ± 0.161
1.069CysSer: 1.069 ± 0.331
0.428CysThr: 0.428 ± 0.214
0.641CysVal: 0.641 ± 0.208
0.071CysTrp: 0.071 ± 0.069
0.499CysTyr: 0.499 ± 0.22
0.0CysXaa: 0.0 ± 0.0
Asp
5.629AspAla: 5.629 ± 0.645
0.499AspCys: 0.499 ± 0.18
3.349AspAsp: 3.349 ± 0.562
4.133AspGlu: 4.133 ± 0.591
2.351AspPhe: 2.351 ± 0.431
4.489AspGly: 4.489 ± 0.902
0.998AspHis: 0.998 ± 0.317
3.135AspIle: 3.135 ± 0.364
2.708AspLys: 2.708 ± 0.512
4.917AspLeu: 4.917 ± 0.472
1.211AspMet: 1.211 ± 0.219
2.921AspAsn: 2.921 ± 0.499
3.064AspPro: 3.064 ± 0.462
1.853AspGln: 1.853 ± 0.38
2.779AspArg: 2.779 ± 0.46
3.634AspSer: 3.634 ± 0.474
3.99AspThr: 3.99 ± 0.747
3.634AspVal: 3.634 ± 0.52
0.926AspTrp: 0.926 ± 0.252
3.777AspTyr: 3.777 ± 0.466
0.0AspXaa: 0.0 ± 0.0
Glu
5.415GluAla: 5.415 ± 0.716
0.57GluCys: 0.57 ± 0.199
3.634GluAsp: 3.634 ± 0.607
5.273GluGlu: 5.273 ± 0.83
3.135GluPhe: 3.135 ± 0.52
3.42GluGly: 3.42 ± 0.427
1.14GluHis: 1.14 ± 0.281
3.634GluIle: 3.634 ± 0.76
2.993GluLys: 2.993 ± 0.564
6.128GluLeu: 6.128 ± 0.761
1.71GluMet: 1.71 ± 0.251
2.494GluAsn: 2.494 ± 0.626
2.138GluPro: 2.138 ± 0.345
3.99GluGln: 3.99 ± 0.602
2.921GluArg: 2.921 ± 0.57
3.206GluSer: 3.206 ± 0.46
3.777GluThr: 3.777 ± 0.521
4.917GluVal: 4.917 ± 0.537
1.354GluTrp: 1.354 ± 0.339
2.138GluTyr: 2.138 ± 0.297
0.0GluXaa: 0.0 ± 0.0
Phe
2.351PheAla: 2.351 ± 0.461
0.428PheCys: 0.428 ± 0.198
2.565PheAsp: 2.565 ± 0.398
2.209PheGlu: 2.209 ± 0.366
1.211PhePhe: 1.211 ± 0.343
2.779PheGly: 2.779 ± 0.473
0.641PheHis: 0.641 ± 0.224
1.781PheIle: 1.781 ± 0.366
1.425PheLys: 1.425 ± 0.338
3.42PheLeu: 3.42 ± 0.599
1.211PheMet: 1.211 ± 0.282
2.066PheAsn: 2.066 ± 0.397
0.855PhePro: 0.855 ± 0.253
1.71PheGln: 1.71 ± 0.376
1.639PheArg: 1.639 ± 0.212
2.636PheSer: 2.636 ± 0.389
2.494PheThr: 2.494 ± 0.392
2.636PheVal: 2.636 ± 0.345
0.214PheTrp: 0.214 ± 0.122
1.639PheTyr: 1.639 ± 0.336
0.0PheXaa: 0.0 ± 0.0
Gly
6.199GlyAla: 6.199 ± 0.87
0.356GlyCys: 0.356 ± 0.148
4.347GlyAsp: 4.347 ± 0.774
3.563GlyGlu: 3.563 ± 0.613
1.71GlyPhe: 1.71 ± 0.404
3.777GlyGly: 3.777 ± 0.809
0.926GlyHis: 0.926 ± 0.219
5.202GlyIle: 5.202 ± 0.665
4.418GlyLys: 4.418 ± 0.656
5.629GlyLeu: 5.629 ± 0.581
1.71GlyMet: 1.71 ± 0.283
3.563GlyAsn: 3.563 ± 0.581
2.138GlyPro: 2.138 ± 0.528
2.494GlyGln: 2.494 ± 0.463
4.062GlyArg: 4.062 ± 0.499
4.204GlySer: 4.204 ± 0.573
5.558GlyThr: 5.558 ± 1.04
5.7GlyVal: 5.7 ± 0.508
1.283GlyTrp: 1.283 ± 0.258
2.921GlyTyr: 2.921 ± 0.482
0.0GlyXaa: 0.0 ± 0.0
His
0.641HisAla: 0.641 ± 0.18
0.356HisCys: 0.356 ± 0.169
0.855HisAsp: 0.855 ± 0.26
1.14HisGlu: 1.14 ± 0.318
0.356HisPhe: 0.356 ± 0.167
1.069HisGly: 1.069 ± 0.303
0.499HisHis: 0.499 ± 0.192
0.998HisIle: 0.998 ± 0.221
0.998HisLys: 0.998 ± 0.353
1.211HisLeu: 1.211 ± 0.322
0.784HisMet: 0.784 ± 0.232
0.784HisAsn: 0.784 ± 0.288
0.57HisPro: 0.57 ± 0.184
0.57HisGln: 0.57 ± 0.216
0.713HisArg: 0.713 ± 0.246
0.641HisSer: 0.641 ± 0.206
0.713HisThr: 0.713 ± 0.215
0.641HisVal: 0.641 ± 0.277
0.214HisTrp: 0.214 ± 0.121
1.069HisTyr: 1.069 ± 0.264
0.0HisXaa: 0.0 ± 0.0
Ile
3.99IleAla: 3.99 ± 0.564
0.499IleCys: 0.499 ± 0.191
3.919IleAsp: 3.919 ± 0.465
3.919IleGlu: 3.919 ± 0.472
1.283IlePhe: 1.283 ± 0.271
3.563IleGly: 3.563 ± 0.539
0.784IleHis: 0.784 ± 0.308
2.921IleIle: 2.921 ± 0.6
3.848IleLys: 3.848 ± 0.647
3.777IleLeu: 3.777 ± 0.567
0.998IleMet: 0.998 ± 0.246
2.565IleAsn: 2.565 ± 0.477
3.135IlePro: 3.135 ± 0.485
1.995IleGln: 1.995 ± 0.335
3.064IleArg: 3.064 ± 0.404
4.418IleSer: 4.418 ± 0.626
3.705IleThr: 3.705 ± 0.478
2.423IleVal: 2.423 ± 0.394
0.428IleTrp: 0.428 ± 0.168
2.066IleTyr: 2.066 ± 0.39
0.0IleXaa: 0.0 ± 0.0
Lys
4.774LysAla: 4.774 ± 0.582
0.57LysCys: 0.57 ± 0.204
3.492LysAsp: 3.492 ± 0.442
4.062LysGlu: 4.062 ± 0.548
1.924LysPhe: 1.924 ± 0.319
3.634LysGly: 3.634 ± 0.578
0.499LysHis: 0.499 ± 0.201
3.777LysIle: 3.777 ± 0.459
2.993LysLys: 2.993 ± 0.481
4.988LysLeu: 4.988 ± 0.591
1.069LysMet: 1.069 ± 0.274
3.705LysAsn: 3.705 ± 0.61
1.71LysPro: 1.71 ± 0.377
2.138LysGln: 2.138 ± 0.414
4.275LysArg: 4.275 ± 0.544
4.062LysSer: 4.062 ± 0.489
2.85LysThr: 2.85 ± 0.362
2.85LysVal: 2.85 ± 0.409
0.713LysTrp: 0.713 ± 0.197
2.494LysTyr: 2.494 ± 0.409
0.0LysXaa: 0.0 ± 0.0
Leu
6.983LeuAla: 6.983 ± 0.81
0.998LeuCys: 0.998 ± 0.263
5.273LeuAsp: 5.273 ± 0.566
6.057LeuGlu: 6.057 ± 0.664
2.494LeuPhe: 2.494 ± 0.403
4.774LeuGly: 4.774 ± 0.473
1.853LeuHis: 1.853 ± 0.411
4.632LeuIle: 4.632 ± 0.731
4.632LeuLys: 4.632 ± 0.631
5.772LeuLeu: 5.772 ± 0.724
2.138LeuMet: 2.138 ± 0.356
4.347LeuAsn: 4.347 ± 0.499
3.705LeuPro: 3.705 ± 0.494
5.13LeuGln: 5.13 ± 0.796
4.489LeuArg: 4.489 ± 0.815
6.199LeuSer: 6.199 ± 0.568
5.273LeuThr: 5.273 ± 0.686
4.632LeuVal: 4.632 ± 0.749
0.855LeuTrp: 0.855 ± 0.273
2.494LeuTyr: 2.494 ± 0.374
0.0LeuXaa: 0.0 ± 0.0
Met
2.351MetAla: 2.351 ± 0.441
0.356MetCys: 0.356 ± 0.241
1.425MetAsp: 1.425 ± 0.272
1.283MetGlu: 1.283 ± 0.339
0.784MetPhe: 0.784 ± 0.235
1.71MetGly: 1.71 ± 0.293
0.285MetHis: 0.285 ± 0.217
0.998MetIle: 0.998 ± 0.256
1.14MetLys: 1.14 ± 0.301
2.565MetLeu: 2.565 ± 0.461
0.641MetMet: 0.641 ± 0.209
1.425MetAsn: 1.425 ± 0.368
0.926MetPro: 0.926 ± 0.255
1.283MetGln: 1.283 ± 0.263
1.496MetArg: 1.496 ± 0.29
1.568MetSer: 1.568 ± 0.293
2.423MetThr: 2.423 ± 0.446
1.568MetVal: 1.568 ± 0.312
0.285MetTrp: 0.285 ± 0.132
0.57MetTyr: 0.57 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
3.705AsnAla: 3.705 ± 0.439
0.356AsnCys: 0.356 ± 0.143
2.494AsnAsp: 2.494 ± 0.359
2.28AsnGlu: 2.28 ± 0.393
2.066AsnPhe: 2.066 ± 0.43
4.204AsnGly: 4.204 ± 0.568
1.069AsnHis: 1.069 ± 0.254
1.995AsnIle: 1.995 ± 0.398
2.993AsnLys: 2.993 ± 0.541
5.344AsnLeu: 5.344 ± 0.405
1.354AsnMet: 1.354 ± 0.317
3.634AsnAsn: 3.634 ± 0.541
3.064AsnPro: 3.064 ± 0.5
2.921AsnGln: 2.921 ± 0.617
3.492AsnArg: 3.492 ± 0.417
2.779AsnSer: 2.779 ± 0.377
3.206AsnThr: 3.206 ± 0.527
3.206AsnVal: 3.206 ± 0.414
0.499AsnTrp: 0.499 ± 0.214
2.636AsnTyr: 2.636 ± 0.448
0.0AsnXaa: 0.0 ± 0.0
Pro
2.494ProAla: 2.494 ± 0.461
0.428ProCys: 0.428 ± 0.159
2.636ProAsp: 2.636 ± 0.475
4.204ProGlu: 4.204 ± 0.654
2.28ProPhe: 2.28 ± 0.32
3.278ProGly: 3.278 ± 0.428
0.285ProHis: 0.285 ± 0.112
2.423ProIle: 2.423 ± 0.483
1.639ProLys: 1.639 ± 0.385
2.565ProLeu: 2.565 ± 0.341
0.784ProMet: 0.784 ± 0.273
2.28ProAsn: 2.28 ± 0.45
1.995ProPro: 1.995 ± 0.4
2.351ProGln: 2.351 ± 0.458
1.568ProArg: 1.568 ± 0.402
3.705ProSer: 3.705 ± 0.525
3.705ProThr: 3.705 ± 0.443
2.921ProVal: 2.921 ± 0.497
0.855ProTrp: 0.855 ± 0.291
0.998ProTyr: 0.998 ± 0.265
0.0ProXaa: 0.0 ± 0.0
Gln
4.632GlnAla: 4.632 ± 0.89
0.214GlnCys: 0.214 ± 0.133
2.494GlnAsp: 2.494 ± 0.418
3.135GlnGlu: 3.135 ± 0.521
2.138GlnPhe: 2.138 ± 0.424
3.777GlnGly: 3.777 ± 0.527
0.57GlnHis: 0.57 ± 0.195
2.636GlnIle: 2.636 ± 0.518
2.565GlnLys: 2.565 ± 0.389
4.845GlnLeu: 4.845 ± 0.688
1.283GlnMet: 1.283 ± 0.329
2.138GlnAsn: 2.138 ± 0.439
1.354GlnPro: 1.354 ± 0.335
4.56GlnGln: 4.56 ± 1.218
2.351GlnArg: 2.351 ± 0.38
2.85GlnSer: 2.85 ± 0.584
3.492GlnThr: 3.492 ± 0.559
2.494GlnVal: 2.494 ± 0.378
0.57GlnTrp: 0.57 ± 0.19
1.639GlnTyr: 1.639 ± 0.332
0.0GlnXaa: 0.0 ± 0.0
Arg
4.489ArgAla: 4.489 ± 0.694
0.499ArgCys: 0.499 ± 0.183
2.565ArgAsp: 2.565 ± 0.37
3.349ArgGlu: 3.349 ± 0.53
1.568ArgPhe: 1.568 ± 0.302
3.206ArgGly: 3.206 ± 0.627
0.57ArgHis: 0.57 ± 0.194
3.135ArgIle: 3.135 ± 0.471
3.99ArgLys: 3.99 ± 0.62
3.919ArgLeu: 3.919 ± 0.479
1.211ArgMet: 1.211 ± 0.239
1.781ArgAsn: 1.781 ± 0.388
1.853ArgPro: 1.853 ± 0.294
2.28ArgGln: 2.28 ± 0.494
2.779ArgArg: 2.779 ± 0.47
3.563ArgSer: 3.563 ± 0.46
3.135ArgThr: 3.135 ± 0.533
2.921ArgVal: 2.921 ± 0.51
0.57ArgTrp: 0.57 ± 0.194
2.28ArgTyr: 2.28 ± 0.402
0.0ArgXaa: 0.0 ± 0.0
Ser
5.487SerAla: 5.487 ± 0.474
0.57SerCys: 0.57 ± 0.21
4.062SerAsp: 4.062 ± 0.446
4.917SerGlu: 4.917 ± 0.523
3.42SerPhe: 3.42 ± 0.437
6.128SerGly: 6.128 ± 0.782
0.998SerHis: 0.998 ± 0.258
3.064SerIle: 3.064 ± 0.432
3.919SerLys: 3.919 ± 0.495
5.344SerLeu: 5.344 ± 0.628
1.496SerMet: 1.496 ± 0.335
4.204SerAsn: 4.204 ± 0.642
3.563SerPro: 3.563 ± 0.513
2.779SerGln: 2.779 ± 0.438
2.921SerArg: 2.921 ± 0.451
4.418SerSer: 4.418 ± 0.497
3.705SerThr: 3.705 ± 0.599
3.634SerVal: 3.634 ± 0.505
0.855SerTrp: 0.855 ± 0.331
2.423SerTyr: 2.423 ± 0.35
0.0SerXaa: 0.0 ± 0.0
Thr
6.199ThrAla: 6.199 ± 0.549
0.641ThrCys: 0.641 ± 0.234
3.563ThrAsp: 3.563 ± 0.457
2.779ThrGlu: 2.779 ± 0.501
1.995ThrPhe: 1.995 ± 0.391
5.344ThrGly: 5.344 ± 0.883
0.926ThrHis: 0.926 ± 0.247
3.064ThrIle: 3.064 ± 0.646
3.349ThrLys: 3.349 ± 0.493
5.558ThrLeu: 5.558 ± 0.402
1.069ThrMet: 1.069 ± 0.247
3.777ThrAsn: 3.777 ± 0.716
4.632ThrPro: 4.632 ± 0.453
2.993ThrGln: 2.993 ± 0.616
1.924ThrArg: 1.924 ± 0.243
4.988ThrSer: 4.988 ± 0.804
5.843ThrThr: 5.843 ± 0.707
4.632ThrVal: 4.632 ± 0.55
0.926ThrTrp: 0.926 ± 0.25
3.206ThrTyr: 3.206 ± 0.592
0.0ThrXaa: 0.0 ± 0.0
Val
5.558ValAla: 5.558 ± 0.803
0.57ValCys: 0.57 ± 0.204
4.632ValAsp: 4.632 ± 0.581
3.492ValGlu: 3.492 ± 0.435
1.354ValPhe: 1.354 ± 0.51
4.845ValGly: 4.845 ± 0.515
0.855ValHis: 0.855 ± 0.238
3.206ValIle: 3.206 ± 0.382
3.848ValLys: 3.848 ± 0.495
3.848ValLeu: 3.848 ± 0.555
1.995ValMet: 1.995 ± 0.308
3.206ValAsn: 3.206 ± 0.48
3.064ValPro: 3.064 ± 0.384
2.565ValGln: 2.565 ± 0.436
2.779ValArg: 2.779 ± 0.38
4.347ValSer: 4.347 ± 0.502
4.703ValThr: 4.703 ± 0.791
4.845ValVal: 4.845 ± 0.697
0.784ValTrp: 0.784 ± 0.228
1.781ValTyr: 1.781 ± 0.292
0.0ValXaa: 0.0 ± 0.0
Trp
0.641TrpAla: 0.641 ± 0.226
0.214TrpCys: 0.214 ± 0.118
0.641TrpAsp: 0.641 ± 0.203
1.14TrpGlu: 1.14 ± 0.358
0.855TrpPhe: 0.855 ± 0.256
0.356TrpGly: 0.356 ± 0.184
0.285TrpHis: 0.285 ± 0.141
0.784TrpIle: 0.784 ± 0.195
0.641TrpLys: 0.641 ± 0.209
1.781TrpLeu: 1.781 ± 0.279
0.285TrpMet: 0.285 ± 0.138
0.713TrpAsn: 0.713 ± 0.212
0.356TrpPro: 0.356 ± 0.139
0.499TrpGln: 0.499 ± 0.249
0.285TrpArg: 0.285 ± 0.138
0.713TrpSer: 0.713 ± 0.178
0.926TrpThr: 0.926 ± 0.235
0.926TrpVal: 0.926 ± 0.247
0.071TrpTrp: 0.071 ± 0.081
0.356TrpTyr: 0.356 ± 0.128
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.138TyrAla: 2.138 ± 0.359
0.214TyrCys: 0.214 ± 0.109
3.349TyrAsp: 3.349 ± 0.498
2.209TyrGlu: 2.209 ± 0.447
1.425TyrPhe: 1.425 ± 0.327
2.636TyrGly: 2.636 ± 0.352
0.641TyrHis: 0.641 ± 0.16
2.138TyrIle: 2.138 ± 0.333
2.779TyrLys: 2.779 ± 0.563
3.42TyrLeu: 3.42 ± 0.456
1.283TyrMet: 1.283 ± 0.404
2.708TyrAsn: 2.708 ± 0.427
1.354TyrPro: 1.354 ± 0.285
2.066TyrGln: 2.066 ± 0.338
1.853TyrArg: 1.853 ± 0.334
2.708TyrSer: 2.708 ± 0.491
1.995TyrThr: 1.995 ± 0.422
2.28TyrVal: 2.28 ± 0.463
0.356TyrTrp: 0.356 ± 0.154
1.069TyrTyr: 1.069 ± 0.321
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (14035 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski