Paenimaribius caenipelagi
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3702 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A547PIS5|A0A547PIS5_9RHOB Uncharacterized protein OS=Paenimaribius caenipelagi OX=2489174 GN=FEV53_19610 PE=4 SV=1
MM1 pKa = 7.85 AEE3 pKa = 4.77 PDD5 pKa = 3.62 TEE7 pKa = 4.3 LTVPLQEE14 pKa = 4.92 QSDD17 pKa = 4.0 LLYY20 pKa = 10.79 FDD22 pKa = 5.77 FNLDD26 pKa = 3.32 GVEE29 pKa = 5.12 DD30 pKa = 4.54 IIQIRR35 pKa = 11.84 RR36 pKa = 11.84 SDD38 pKa = 4.02 SVWTGTQFLDD48 pKa = 2.79 IWSVEE53 pKa = 3.89 FWEE56 pKa = 4.69 VGSLSDD62 pKa = 4.89 PGFSHH67 pKa = 7.62 PDD69 pKa = 3.08 DD70 pKa = 3.81 VAILHH75 pKa = 6.36 PVTVEE80 pKa = 3.66 AVKK83 pKa = 10.9 YY84 pKa = 9.87 LASADD89 pKa = 3.97 FNKK92 pKa = 10.68 DD93 pKa = 2.53 LYY95 pKa = 11.43 NDD97 pKa = 3.32 VKK99 pKa = 10.83 RR100 pKa = 11.84 PHH102 pKa = 7.5 DD103 pKa = 3.78 YY104 pKa = 11.31 AQTLTLADD112 pKa = 4.56 DD113 pKa = 4.7 LNSDD117 pKa = 4.48 GYY119 pKa = 11.85 VDD121 pKa = 4.18 LQFSVGFNYY130 pKa = 10.0 EE131 pKa = 3.92 DD132 pKa = 3.71 PLYY135 pKa = 10.56 GSRR138 pKa = 11.84 YY139 pKa = 9.84 EE140 pKa = 4.0 YY141 pKa = 10.02 LTEE144 pKa = 4.29 NGIEE148 pKa = 3.97 FAGYY152 pKa = 9.66 ILDD155 pKa = 3.6 NGRR158 pKa = 11.84 YY159 pKa = 8.48 QDD161 pKa = 3.87 HH162 pKa = 6.99 TDD164 pKa = 4.2 DD165 pKa = 5.05 FYY167 pKa = 11.85 GDD169 pKa = 4.08 PPHH172 pKa = 6.36 QLSEE176 pKa = 4.21 IIQAFEE182 pKa = 3.91 RR183 pKa = 11.84 VISGSVDD190 pKa = 3.57 LDD192 pKa = 3.43 EE193 pKa = 7.21 DD194 pKa = 3.94 GALEE198 pKa = 4.11 KK199 pKa = 10.52 FAVLDD204 pKa = 3.87 FQSRR208 pKa = 11.84 SWGSLYY214 pKa = 11.0 DD215 pKa = 3.73 GDD217 pKa = 4.89 SNIIEE222 pKa = 4.18 SWDD225 pKa = 3.61 RR226 pKa = 11.84 QQSANYY232 pKa = 9.1 VEE234 pKa = 5.82 LYY236 pKa = 10.84 EE237 pKa = 5.99 FDD239 pKa = 3.95 DD240 pKa = 4.67 QLAIWRR246 pKa = 11.84 DD247 pKa = 3.39 SEE249 pKa = 4.84 EE250 pKa = 4.31 YY251 pKa = 10.33 PNTPVPLAYY260 pKa = 10.61 DD261 pKa = 3.95 NVWGLIPTLIFDD273 pKa = 4.63 SDD275 pKa = 3.89 DD276 pKa = 3.85 PLNVEE281 pKa = 4.39 QVMSTLSRR289 pKa = 11.84 LPGYY293 pKa = 7.58 GTQGFYY299 pKa = 10.86 EE300 pKa = 4.66 GLSSYY305 pKa = 11.03 DD306 pKa = 2.99 WYY308 pKa = 9.8 TAYY311 pKa = 10.5 HH312 pKa = 6.76 DD313 pKa = 4.43 WYY315 pKa = 8.69 TGPGGSVDD323 pKa = 3.75 YY324 pKa = 11.29 DD325 pKa = 4.05 AMFLFANSINANQDD339 pKa = 2.88 PTFQLGMTQALLQDD353 pKa = 4.23 ALDD356 pKa = 3.81 SRR358 pKa = 11.84 GGSISVDD365 pKa = 3.21 LDD367 pKa = 3.54 EE368 pKa = 6.81 FITDD372 pKa = 4.38 PDD374 pKa = 3.87 PTGDD378 pKa = 3.85 PLTYY382 pKa = 10.46 SAIDD386 pKa = 3.8 LPAGLILDD394 pKa = 3.98 SSTGVVSGASEE405 pKa = 4.05 EE406 pKa = 4.49 TMTTSFMADD415 pKa = 3.47 DD416 pKa = 4.12 GRR418 pKa = 11.84 GGTFTTTVSFSLPGLDD434 pKa = 4.74 GIVDD438 pKa = 4.26 GTAGGDD444 pKa = 3.49 VITTNFVDD452 pKa = 4.64 AYY454 pKa = 10.46 GDD456 pKa = 3.39 TLTFSKK462 pKa = 10.65 VDD464 pKa = 3.59 HH465 pKa = 6.58 KK466 pKa = 11.61 VLGYY470 pKa = 10.77 EE471 pKa = 3.87 GDD473 pKa = 3.93 DD474 pKa = 3.69 YY475 pKa = 11.66 IGIAKK480 pKa = 10.32 GIGIEE485 pKa = 4.11 VSGGAGNDD493 pKa = 3.37 VIRR496 pKa = 11.84 INSATGTASGDD507 pKa = 3.5 GGNDD511 pKa = 3.67 TIHH514 pKa = 6.19 TGVRR518 pKa = 11.84 SMVVDD523 pKa = 4.28 GGTGDD528 pKa = 4.93 DD529 pKa = 3.42 MLIANMCYY537 pKa = 9.81 GAKK540 pKa = 8.47 HH541 pKa = 5.68 TLTGGEE547 pKa = 4.17 GADD550 pKa = 3.16 TFVFTAASTRR560 pKa = 11.84 KK561 pKa = 8.97 MSVATVTDD569 pKa = 4.38 FDD571 pKa = 4.9 PEE573 pKa = 4.52 LDD575 pKa = 3.57 TLVIEE580 pKa = 4.8 GEE582 pKa = 4.42 VVDD585 pKa = 6.55 LSRR588 pKa = 11.84 AWLYY592 pKa = 10.86 DD593 pKa = 3.17 FRR595 pKa = 11.84 SITTTAGGDD604 pKa = 3.23 RR605 pKa = 11.84 VLGYY609 pKa = 10.82 GDD611 pKa = 3.56 NDD613 pKa = 3.91 EE614 pKa = 4.92 IVISNLIDD622 pKa = 3.57 VDD624 pKa = 3.84 DD625 pKa = 5.26 LLYY628 pKa = 10.91 QFF630 pKa = 5.61
Molecular weight: 68.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.617
IPC_protein 3.681
Toseland 3.439
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.49
Grimsley 3.338
Solomon 3.681
Lehninger 3.63
Nozaki 3.783
DTASelect 4.075
Thurlkill 3.49
EMBOSS 3.643
Sillero 3.795
Patrickios 0.858
IPC_peptide 3.668
IPC2_peptide 3.77
IPC2.peptide.svr19 3.725
Protein with the highest isoelectric point:
>tr|A0A547Q757|A0A547Q757_9RHOB MBL fold metallo-hydrolase OS=Paenimaribius caenipelagi OX=2489174 GN=FEV53_05705 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3702
0
3702
1141485
32
2956
308.3
33.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.803 ± 0.05
0.937 ± 0.017
6.268 ± 0.045
6.119 ± 0.041
3.578 ± 0.027
8.763 ± 0.059
2.054 ± 0.023
5.176 ± 0.028
2.99 ± 0.032
10.04 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.603 ± 0.021
2.553 ± 0.024
5.096 ± 0.031
3.155 ± 0.02
7.152 ± 0.048
5.392 ± 0.029
5.574 ± 0.031
7.137 ± 0.037
1.398 ± 0.015
2.211 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here