SAR202 cluster bacterium AC-647-P02_OGT_505m

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexi incertae sedis; SAR202 cluster

Average proteome isoelectric point is 5.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1332 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N8YM26|A0A5N8YM26_9CHLR Uncharacterized protein OS=SAR202 cluster bacterium AC-647-P02_OGT_505m OX=2587832 GN=FIM09_06470 PE=3 SV=1
MM1 pKa = 6.88AQYY4 pKa = 10.01MVVHH8 pKa = 6.28SLKK11 pKa = 10.7VPADD15 pKa = 3.53QFSQAMEE22 pKa = 4.93DD23 pKa = 3.65DD24 pKa = 4.82SITEE28 pKa = 3.89FAKK31 pKa = 10.71AMQAGEE37 pKa = 4.37TPAKK41 pKa = 9.99CVKK44 pKa = 9.59SWNPLPYY51 pKa = 10.73GNTDD55 pKa = 3.15TFICLWEE62 pKa = 4.53ANNEE66 pKa = 3.83EE67 pKa = 5.21DD68 pKa = 4.48IVATLGEE75 pKa = 4.8MIEE78 pKa = 4.66MITCEE83 pKa = 3.96PMEE86 pKa = 4.26VDD88 pKa = 4.23EE89 pKa = 5.74IDD91 pKa = 3.23WAQIAASS98 pKa = 3.64

Molecular weight: 10.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N8YKK7|A0A5N8YKK7_9CHLR Mandelate racemase OS=SAR202 cluster bacterium AC-647-P02_OGT_505m OX=2587832 GN=FIM09_02990 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.18ISSPVLKK9 pKa = 10.78LRR11 pKa = 11.84DD12 pKa = 3.77FRR14 pKa = 11.84MLIAATFFEE23 pKa = 4.65SFMRR27 pKa = 11.84GEE29 pKa = 4.13NVVLGWVVLEE39 pKa = 4.29MTDD42 pKa = 3.41SPFMVGLTMGIRR54 pKa = 11.84AAPGFFLGITAGTISDD70 pKa = 3.71LVDD73 pKa = 2.87RR74 pKa = 11.84RR75 pKa = 11.84KK76 pKa = 10.34FMRR79 pKa = 11.84LLMALAALVALTMGLLLASGNVEE102 pKa = 4.1LWHH105 pKa = 6.68LLAIPVFSGTIHH117 pKa = 6.36MMFNTAKK124 pKa = 10.16QSFVFDD130 pKa = 3.46LVGRR134 pKa = 11.84EE135 pKa = 3.73NGLNGMSYY143 pKa = 10.73LGLSMRR149 pKa = 11.84GGGMLGALGVGFALARR165 pKa = 11.84WGAGAGYY172 pKa = 9.42FVVAVACCISVGFIGFIRR190 pKa = 11.84SRR192 pKa = 11.84GKK194 pKa = 9.87SAPSRR199 pKa = 11.84KK200 pKa = 9.93GNILTGFSEE209 pKa = 5.49FWRR212 pKa = 11.84EE213 pKa = 3.45MRR215 pKa = 11.84QNRR218 pKa = 11.84TIAGLVLIVMLVEE231 pKa = 4.71FFGFTPNNALMPSIARR247 pKa = 11.84DD248 pKa = 3.4VWGLGPQGLGILNAFSSGGGMLAIAVISLVGHH280 pKa = 6.64IRR282 pKa = 11.84YY283 pKa = 9.3QGLAFIVVLHH293 pKa = 6.23LFGGALLLLGFAPSVYY309 pKa = 9.86VAVIAIVILSGTMALSDD326 pKa = 4.64LFSQTLLQRR335 pKa = 11.84LVPNDD340 pKa = 3.1LRR342 pKa = 11.84GRR344 pKa = 11.84IMGAWTTAVGTSPVGNLEE362 pKa = 3.72IGALASFMGVTVALAFHH379 pKa = 6.72GGGLVLLAVITLLAFKK395 pKa = 10.27RR396 pKa = 11.84LRR398 pKa = 11.84SII400 pKa = 4.24

Molecular weight: 42.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1332

0

1332

411302

27

1494

308.8

33.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.59 ± 0.059

0.908 ± 0.02

5.902 ± 0.059

6.599 ± 0.064

4.001 ± 0.051

8.436 ± 0.049

2.022 ± 0.03

7.232 ± 0.058

4.327 ± 0.047

9.113 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.758 ± 0.026

3.979 ± 0.035

4.58 ± 0.04

2.921 ± 0.03

5.046 ± 0.047

7.547 ± 0.058

5.452 ± 0.037

7.401 ± 0.051

1.334 ± 0.029

2.852 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski