Rhodospirillum centenum (strain ATCC 51521 / SW)
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3984 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B6IMI6|B6IMI6_RHOCS Sulfurtransferase OS=Rhodospirillum centenum (strain ATCC 51521 / SW) OX=414684 GN=sseA PE=4 SV=1
MM1 pKa = 6.9 VAEE4 pKa = 4.07 GLIRR8 pKa = 11.84 VDD10 pKa = 6.39 SEE12 pKa = 4.36 FQWDD16 pKa = 4.0 NTWDD20 pKa = 3.35 EE21 pKa = 4.29 PYY23 pKa = 10.72 AYY25 pKa = 9.58 GVYY28 pKa = 10.29 HH29 pKa = 7.18 IDD31 pKa = 3.62 FGQGRR36 pKa = 11.84 VVEE39 pKa = 4.16 LLGGDD44 pKa = 4.39 FGAGGLTAANLTFFGTFAA62 pKa = 4.65
Molecular weight: 6.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.961
IPC_protein 3.808
Toseland 3.617
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.656
Grimsley 3.541
Solomon 3.77
Lehninger 3.719
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.935
Patrickios 1.901
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.827
Protein with the highest isoelectric point:
>tr|B6IPR6|B6IPR6_RHOCS Fido domain-containing protein OS=Rhodospirillum centenum (strain ATCC 51521 / SW) OX=414684 GN=RC1_2383 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.84 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.16 RR41 pKa = 11.84 LSAA44 pKa = 3.93
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3984
0
3984
1255268
37
5368
315.1
33.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.499 ± 0.062
0.79 ± 0.011
5.87 ± 0.035
5.653 ± 0.038
3.257 ± 0.024
9.213 ± 0.048
1.923 ± 0.018
3.981 ± 0.029
2.246 ± 0.03
10.895 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.144 ± 0.021
1.979 ± 0.02
6.106 ± 0.041
2.987 ± 0.025
8.42 ± 0.048
4.528 ± 0.035
5.435 ± 0.056
7.765 ± 0.033
1.344 ± 0.018
1.965 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here