Amino acid dipepetide frequency for Eel River basin pequenovirus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.937AlaAla: 4.937 ± 1.911
1.097AlaCys: 1.097 ± 0.796
3.291AlaAsp: 3.291 ± 1.512
1.097AlaGlu: 1.097 ± 0.677
4.388AlaPhe: 4.388 ± 1.089
8.777AlaGly: 8.777 ± 3.014
0.0AlaHis: 0.0 ± 0.0
5.485AlaIle: 5.485 ± 1.593
5.485AlaLys: 5.485 ± 2.628
4.937AlaLeu: 4.937 ± 2.601
2.743AlaMet: 2.743 ± 1.22
2.194AlaAsn: 2.194 ± 1.193
2.194AlaPro: 2.194 ± 1.197
2.194AlaGln: 2.194 ± 0.77
3.291AlaArg: 3.291 ± 1.182
3.291AlaSer: 3.291 ± 1.805
5.485AlaThr: 5.485 ± 2.034
4.937AlaVal: 4.937 ± 0.896
1.646AlaTrp: 1.646 ± 1.797
0.549AlaTyr: 0.549 ± 0.467
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.549CysCys: 0.549 ± 0.467
0.0CysAsp: 0.0 ± 0.0
1.097CysGlu: 1.097 ± 0.888
0.0CysPhe: 0.0 ± 0.0
0.549CysGly: 0.549 ± 0.605
0.549CysHis: 0.549 ± 0.467
0.549CysIle: 0.549 ± 0.482
0.549CysLys: 0.549 ± 0.482
1.646CysLeu: 1.646 ± 0.613
0.549CysMet: 0.549 ± 0.415
0.549CysAsn: 0.549 ± 0.591
0.0CysPro: 0.0 ± 0.0
0.0CysGln: 0.0 ± 0.0
1.097CysArg: 1.097 ± 0.677
0.0CysSer: 0.0 ± 0.0
0.549CysThr: 0.549 ± 0.467
0.549CysVal: 0.549 ± 0.482
0.549CysTrp: 0.549 ± 0.599
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
4.937AspAla: 4.937 ± 1.261
0.0AspCys: 0.0 ± 0.0
4.937AspAsp: 4.937 ± 2.7
4.937AspGlu: 4.937 ± 1.887
1.097AspPhe: 1.097 ± 0.659
1.646AspGly: 1.646 ± 0.98
1.646AspHis: 1.646 ± 0.969
2.194AspIle: 2.194 ± 0.917
2.743AspLys: 2.743 ± 1.359
4.937AspLeu: 4.937 ± 1.543
0.0AspMet: 0.0 ± 0.0
2.743AspAsn: 2.743 ± 1.436
2.194AspPro: 2.194 ± 0.96
1.646AspGln: 1.646 ± 0.924
1.646AspArg: 1.646 ± 0.805
5.485AspSer: 5.485 ± 2.224
2.194AspThr: 2.194 ± 1.276
2.743AspVal: 2.743 ± 1.382
0.0AspTrp: 0.0 ± 0.0
3.291AspTyr: 3.291 ± 1.0
0.0AspXaa: 0.0 ± 0.0
Glu
0.549GluAla: 0.549 ± 0.57
0.549GluCys: 0.549 ± 0.482
0.549GluAsp: 0.549 ± 0.605
4.937GluGlu: 4.937 ± 2.682
2.194GluPhe: 2.194 ± 1.076
3.84GluGly: 3.84 ± 1.38
1.646GluHis: 1.646 ± 1.145
5.485GluIle: 5.485 ± 1.56
9.325GluLys: 9.325 ± 3.041
4.937GluLeu: 4.937 ± 1.249
1.646GluMet: 1.646 ± 0.879
2.194GluAsn: 2.194 ± 0.662
1.097GluPro: 1.097 ± 0.935
1.097GluGln: 1.097 ± 0.716
4.388GluArg: 4.388 ± 1.265
3.291GluSer: 3.291 ± 1.497
2.743GluThr: 2.743 ± 0.755
4.937GluVal: 4.937 ± 1.997
0.549GluTrp: 0.549 ± 0.467
4.937GluTyr: 4.937 ± 2.265
0.0GluXaa: 0.0 ± 0.0
Phe
1.646PheAla: 1.646 ± 0.91
0.0PheCys: 0.0 ± 0.0
0.549PheAsp: 0.549 ± 0.591
3.291PheGlu: 3.291 ± 1.348
0.549PhePhe: 0.549 ± 0.605
2.743PheGly: 2.743 ± 0.906
0.549PheHis: 0.549 ± 0.482
2.743PheIle: 2.743 ± 1.132
3.291PheLys: 3.291 ± 1.725
2.743PheLeu: 2.743 ± 1.835
0.0PheMet: 0.0 ± 0.0
4.388PheAsn: 4.388 ± 1.756
2.194PhePro: 2.194 ± 0.852
1.097PheGln: 1.097 ± 0.645
1.646PheArg: 1.646 ± 0.959
3.84PheSer: 3.84 ± 0.78
2.743PheThr: 2.743 ± 1.33
1.097PheVal: 1.097 ± 0.814
0.0PheTrp: 0.0 ± 0.0
1.097PheTyr: 1.097 ± 0.664
0.0PheXaa: 0.0 ± 0.0
Gly
4.388GlyAla: 4.388 ± 2.066
0.549GlyCys: 0.549 ± 0.482
2.743GlyAsp: 2.743 ± 1.607
1.646GlyGlu: 1.646 ± 0.825
1.097GlyPhe: 1.097 ± 0.645
0.0GlyGly: 0.0 ± 0.0
1.097GlyHis: 1.097 ± 0.659
4.388GlyIle: 4.388 ± 1.624
3.84GlyLys: 3.84 ± 1.439
3.291GlyLeu: 3.291 ± 1.821
0.549GlyMet: 0.549 ± 0.591
3.291GlyAsn: 3.291 ± 0.927
0.0GlyPro: 0.0 ± 0.0
1.646GlyGln: 1.646 ± 0.665
2.194GlyArg: 2.194 ± 1.354
6.583GlySer: 6.583 ± 1.276
7.68GlyThr: 7.68 ± 2.049
4.388GlyVal: 4.388 ± 1.136
0.549GlyTrp: 0.549 ± 0.482
4.388GlyTyr: 4.388 ± 1.644
0.0GlyXaa: 0.0 ± 0.0
His
0.549HisAla: 0.549 ± 0.467
0.0HisCys: 0.0 ± 0.0
0.549HisAsp: 0.549 ± 0.523
1.646HisGlu: 1.646 ± 1.087
0.0HisPhe: 0.0 ± 0.0
0.549HisGly: 0.549 ± 0.467
0.0HisHis: 0.0 ± 0.0
0.549HisIle: 0.549 ± 0.482
1.097HisLys: 1.097 ± 0.559
1.097HisLeu: 1.097 ± 0.856
1.097HisMet: 1.097 ± 0.559
0.549HisAsn: 0.549 ± 0.605
2.194HisPro: 2.194 ± 1.371
0.0HisGln: 0.0 ± 0.0
0.549HisArg: 0.549 ± 0.599
1.097HisSer: 1.097 ± 0.827
1.097HisThr: 1.097 ± 0.664
3.291HisVal: 3.291 ± 1.245
0.549HisTrp: 0.549 ± 0.467
0.549HisTyr: 0.549 ± 0.467
0.0HisXaa: 0.0 ± 0.0
Ile
8.777IleAla: 8.777 ± 1.6
1.097IleCys: 1.097 ± 0.664
2.743IleAsp: 2.743 ± 1.058
4.937IleGlu: 4.937 ± 1.656
4.937IlePhe: 4.937 ± 1.406
4.937IleGly: 4.937 ± 1.644
3.84IleHis: 3.84 ± 1.453
3.291IleIle: 3.291 ± 1.426
4.937IleLys: 4.937 ± 1.68
4.388IleLeu: 4.388 ± 1.356
1.646IleMet: 1.646 ± 0.934
4.937IleAsn: 4.937 ± 1.4
3.84IlePro: 3.84 ± 1.052
2.194IleGln: 2.194 ± 1.06
1.646IleArg: 1.646 ± 0.591
3.84IleSer: 3.84 ± 1.933
2.194IleThr: 2.194 ± 0.782
3.291IleVal: 3.291 ± 1.389
2.194IleTrp: 2.194 ± 0.852
3.291IleTyr: 3.291 ± 0.908
0.0IleXaa: 0.0 ± 0.0
Lys
5.485LysAla: 5.485 ± 1.14
0.0LysCys: 0.0 ± 0.0
3.291LysAsp: 3.291 ± 0.784
8.228LysGlu: 8.228 ± 3.478
2.743LysPhe: 2.743 ± 1.898
4.388LysGly: 4.388 ± 2.494
0.0LysHis: 0.0 ± 0.0
8.777LysIle: 8.777 ± 2.912
10.971LysLys: 10.971 ± 4.478
6.583LysLeu: 6.583 ± 1.924
2.743LysMet: 2.743 ± 0.712
7.131LysAsn: 7.131 ± 2.895
1.097LysPro: 1.097 ± 0.965
2.743LysGln: 2.743 ± 1.181
3.84LysArg: 3.84 ± 1.372
7.68LysSer: 7.68 ± 2.458
4.388LysThr: 4.388 ± 1.792
3.84LysVal: 3.84 ± 0.948
1.097LysTrp: 1.097 ± 0.559
4.388LysTyr: 4.388 ± 1.684
0.0LysXaa: 0.0 ± 0.0
Leu
4.388LeuAla: 4.388 ± 3.053
1.097LeuCys: 1.097 ± 0.965
7.131LeuAsp: 7.131 ± 2.463
9.325LeuGlu: 9.325 ± 2.639
3.291LeuPhe: 3.291 ± 1.485
6.034LeuGly: 6.034 ± 1.502
1.097LeuHis: 1.097 ± 0.704
3.84LeuIle: 3.84 ± 0.862
9.325LeuLys: 9.325 ± 2.958
6.583LeuLeu: 6.583 ± 2.191
1.097LeuMet: 1.097 ± 0.677
2.743LeuAsn: 2.743 ± 0.88
2.194LeuPro: 2.194 ± 1.354
3.84LeuGln: 3.84 ± 2.116
3.291LeuArg: 3.291 ± 0.99
8.228LeuSer: 8.228 ± 1.582
5.485LeuThr: 5.485 ± 2.54
3.84LeuVal: 3.84 ± 2.142
0.0LeuTrp: 0.0 ± 0.0
4.388LeuTyr: 4.388 ± 1.375
0.0LeuXaa: 0.0 ± 0.0
Met
0.549MetAla: 0.549 ± 0.599
0.0MetCys: 0.0 ± 0.0
0.0MetAsp: 0.0 ± 0.0
2.194MetGlu: 2.194 ± 1.039
1.646MetPhe: 1.646 ± 1.34
1.097MetGly: 1.097 ± 0.814
0.0MetHis: 0.0 ± 0.0
2.194MetIle: 2.194 ± 1.007
4.937MetLys: 4.937 ± 1.754
3.291MetLeu: 3.291 ± 1.838
0.549MetMet: 0.549 ± 0.482
0.0MetAsn: 0.0 ± 0.0
0.549MetPro: 0.549 ± 0.57
0.549MetGln: 0.549 ± 0.532
2.743MetArg: 2.743 ± 0.786
1.646MetSer: 1.646 ± 1.007
1.646MetThr: 1.646 ± 1.447
0.549MetVal: 0.549 ± 0.467
0.0MetTrp: 0.0 ± 0.0
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
4.937AsnAla: 4.937 ± 2.539
1.646AsnCys: 1.646 ± 0.969
3.291AsnAsp: 3.291 ± 0.989
3.84AsnGlu: 3.84 ± 1.353
1.646AsnPhe: 1.646 ± 0.837
1.646AsnGly: 1.646 ± 1.447
1.097AsnHis: 1.097 ± 0.796
6.583AsnIle: 6.583 ± 1.838
6.034AsnLys: 6.034 ± 2.793
4.388AsnLeu: 4.388 ± 0.752
1.646AsnMet: 1.646 ± 0.867
2.194AsnAsn: 2.194 ± 1.718
2.194AsnPro: 2.194 ± 0.984
3.84AsnGln: 3.84 ± 1.309
3.291AsnArg: 3.291 ± 0.99
2.743AsnSer: 2.743 ± 1.508
3.84AsnThr: 3.84 ± 0.724
3.291AsnVal: 3.291 ± 1.02
0.549AsnTrp: 0.549 ± 0.482
2.743AsnTyr: 2.743 ± 1.678
0.0AsnXaa: 0.0 ± 0.0
Pro
2.194ProAla: 2.194 ± 1.536
0.549ProCys: 0.549 ± 0.599
1.646ProAsp: 1.646 ± 0.675
1.097ProGlu: 1.097 ± 0.664
0.549ProPhe: 0.549 ± 0.467
1.097ProGly: 1.097 ± 0.716
0.0ProHis: 0.0 ± 0.0
1.646ProIle: 1.646 ± 1.162
2.743ProLys: 2.743 ± 1.202
2.743ProLeu: 2.743 ± 1.84
0.549ProMet: 0.549 ± 0.467
3.291ProAsn: 3.291 ± 1.412
0.549ProPro: 0.549 ± 0.467
0.549ProGln: 0.549 ± 0.467
2.194ProArg: 2.194 ± 0.818
1.097ProSer: 1.097 ± 0.659
2.743ProThr: 2.743 ± 1.805
1.646ProVal: 1.646 ± 0.623
1.097ProTrp: 1.097 ± 0.814
1.646ProTyr: 1.646 ± 0.98
0.0ProXaa: 0.0 ± 0.0
Gln
3.291GlnAla: 3.291 ± 1.156
0.0GlnCys: 0.0 ± 0.0
1.646GlnAsp: 1.646 ± 0.907
1.646GlnGlu: 1.646 ± 1.007
1.646GlnPhe: 1.646 ± 1.145
1.646GlnGly: 1.646 ± 0.675
0.0GlnHis: 0.0 ± 0.0
3.84GlnIle: 3.84 ± 1.751
2.194GlnLys: 2.194 ± 0.852
5.485GlnLeu: 5.485 ± 1.605
0.0GlnMet: 0.0 ± 0.0
1.646GlnAsn: 1.646 ± 1.074
1.097GlnPro: 1.097 ± 0.559
2.743GlnGln: 2.743 ± 1.608
1.646GlnArg: 1.646 ± 0.937
2.743GlnSer: 2.743 ± 1.485
2.194GlnThr: 2.194 ± 1.353
0.549GlnVal: 0.549 ± 0.523
1.097GlnTrp: 1.097 ± 0.935
1.646GlnTyr: 1.646 ± 1.202
0.0GlnXaa: 0.0 ± 0.0
Arg
2.194ArgAla: 2.194 ± 0.791
0.549ArgCys: 0.549 ± 0.467
0.0ArgAsp: 0.0 ± 0.0
1.097ArgGlu: 1.097 ± 0.559
4.388ArgPhe: 4.388 ± 1.257
2.743ArgGly: 2.743 ± 1.419
2.743ArgHis: 2.743 ± 1.358
3.291ArgIle: 3.291 ± 1.047
4.388ArgLys: 4.388 ± 1.208
6.034ArgLeu: 6.034 ± 1.93
1.646ArgMet: 1.646 ± 1.074
3.291ArgAsn: 3.291 ± 1.556
1.646ArgPro: 1.646 ± 0.78
2.194ArgGln: 2.194 ± 0.976
1.646ArgArg: 1.646 ± 0.934
2.743ArgSer: 2.743 ± 1.345
2.743ArgThr: 2.743 ± 1.461
1.646ArgVal: 1.646 ± 0.692
0.0ArgTrp: 0.0 ± 0.0
3.291ArgTyr: 3.291 ± 1.034
0.0ArgXaa: 0.0 ± 0.0
Ser
7.131SerAla: 7.131 ± 3.601
0.0SerCys: 0.0 ± 0.0
2.194SerAsp: 2.194 ± 1.135
3.291SerGlu: 3.291 ± 1.117
1.097SerPhe: 1.097 ± 0.888
3.291SerGly: 3.291 ± 1.838
0.549SerHis: 0.549 ± 0.467
5.485SerIle: 5.485 ± 1.603
4.937SerLys: 4.937 ± 1.832
9.874SerLeu: 9.874 ± 3.404
3.291SerMet: 3.291 ± 1.211
8.777SerAsn: 8.777 ± 2.067
1.097SerPro: 1.097 ± 0.602
3.84SerGln: 3.84 ± 1.257
1.646SerArg: 1.646 ± 0.692
5.485SerSer: 5.485 ± 2.269
4.937SerThr: 4.937 ± 2.625
3.84SerVal: 3.84 ± 1.349
0.0SerTrp: 0.0 ± 0.0
1.097SerTyr: 1.097 ± 0.935
0.0SerXaa: 0.0 ± 0.0
Thr
4.388ThrAla: 4.388 ± 2.043
0.0ThrCys: 0.0 ± 0.0
4.937ThrAsp: 4.937 ± 3.055
2.194ThrGlu: 2.194 ± 0.966
1.097ThrPhe: 1.097 ± 0.697
1.646ThrGly: 1.646 ± 0.969
1.097ThrHis: 1.097 ± 0.559
4.388ThrIle: 4.388 ± 1.007
3.84ThrLys: 3.84 ± 2.347
7.68ThrLeu: 7.68 ± 1.725
1.097ThrMet: 1.097 ± 0.965
3.84ThrAsn: 3.84 ± 1.118
2.194ThrPro: 2.194 ± 1.045
4.388ThrGln: 4.388 ± 1.266
4.388ThrArg: 4.388 ± 1.362
6.034ThrSer: 6.034 ± 1.944
4.937ThrThr: 4.937 ± 1.785
2.194ThrVal: 2.194 ± 1.728
1.646ThrTrp: 1.646 ± 0.91
3.84ThrTyr: 3.84 ± 1.31
0.0ThrXaa: 0.0 ± 0.0
Val
4.937ValAla: 4.937 ± 1.214
0.549ValCys: 0.549 ± 0.591
7.68ValAsp: 7.68 ± 2.106
2.194ValGlu: 2.194 ± 1.007
1.097ValPhe: 1.097 ± 0.704
2.743ValGly: 2.743 ± 0.815
0.549ValHis: 0.549 ± 0.605
3.84ValIle: 3.84 ± 1.093
4.388ValLys: 4.388 ± 1.045
2.743ValLeu: 2.743 ± 1.276
1.646ValMet: 1.646 ± 1.275
4.937ValAsn: 4.937 ± 2.116
1.646ValPro: 1.646 ± 0.675
1.646ValGln: 1.646 ± 0.895
1.646ValArg: 1.646 ± 0.934
3.291ValSer: 3.291 ± 1.131
4.388ValThr: 4.388 ± 1.758
2.743ValVal: 2.743 ± 0.866
0.549ValTrp: 0.549 ± 0.467
1.646ValTyr: 1.646 ± 0.98
0.0ValXaa: 0.0 ± 0.0
Trp
0.549TrpAla: 0.549 ± 0.605
0.0TrpCys: 0.0 ± 0.0
0.0TrpAsp: 0.0 ± 0.0
1.097TrpGlu: 1.097 ± 0.64
1.097TrpPhe: 1.097 ± 0.645
0.549TrpGly: 0.549 ± 0.467
0.0TrpHis: 0.0 ± 0.0
0.549TrpIle: 0.549 ± 0.467
1.646TrpLys: 1.646 ± 0.692
0.549TrpLeu: 0.549 ± 0.467
0.0TrpMet: 0.0 ± 0.0
0.549TrpAsn: 0.549 ± 0.467
0.0TrpPro: 0.0 ± 0.0
0.549TrpGln: 0.549 ± 0.467
1.646TrpArg: 1.646 ± 1.447
1.646TrpSer: 1.646 ± 1.402
1.646TrpThr: 1.646 ± 1.162
0.549TrpVal: 0.549 ± 0.599
0.0TrpTrp: 0.0 ± 0.0
1.097TrpTyr: 1.097 ± 0.559
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.194TyrAla: 2.194 ± 0.599
1.097TyrCys: 1.097 ± 0.657
3.84TyrAsp: 3.84 ± 1.676
1.097TyrGlu: 1.097 ± 0.677
1.646TyrPhe: 1.646 ± 0.91
4.388TyrGly: 4.388 ± 1.179
0.549TyrHis: 0.549 ± 0.599
3.84TyrIle: 3.84 ± 1.182
3.291TyrLys: 3.291 ± 1.047
3.291TyrLeu: 3.291 ± 1.208
1.097TyrMet: 1.097 ± 0.965
2.194TyrAsn: 2.194 ± 1.705
1.646TyrPro: 1.646 ± 1.394
0.0TyrGln: 0.0 ± 0.0
3.84TyrArg: 3.84 ± 0.637
1.097TyrSer: 1.097 ± 0.657
2.194TyrThr: 2.194 ± 1.869
4.937TyrVal: 4.937 ± 1.205
1.646TyrTrp: 1.646 ± 0.999
3.291TyrTyr: 3.291 ± 2.14
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 9 proteins (1824 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski