Gordonia phage Sour

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sourvirus; Gordonia virus Sour

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UKW7|A0A2U8UKW7_9CAUD Uncharacterized protein OS=Gordonia phage Sour OX=2182349 GN=74 PE=4 SV=1
MM1 pKa = 7.29NVPTTRR7 pKa = 11.84AEE9 pKa = 3.86EE10 pKa = 3.88TGTYY14 pKa = 9.64KK15 pKa = 10.5RR16 pKa = 11.84PKK18 pKa = 8.26WQPTDD23 pKa = 3.22KK24 pKa = 10.91RR25 pKa = 11.84GVALLDD31 pKa = 3.39HH32 pKa = 6.3VMEE35 pKa = 4.87VIEE38 pKa = 4.2EE39 pKa = 4.14EE40 pKa = 4.14ARR42 pKa = 11.84EE43 pKa = 3.9AAQARR48 pKa = 11.84QVSAAWLDD56 pKa = 3.27EE57 pKa = 4.05DD58 pKa = 4.42TRR60 pKa = 11.84AGHH63 pKa = 6.92WDD65 pKa = 3.26QGDD68 pKa = 4.11WIQLNGAALDD78 pKa = 3.96DD79 pKa = 4.68AGVNVLALALEE90 pKa = 4.18QEE92 pKa = 4.67GLSLTEE98 pKa = 4.65ALEE101 pKa = 3.75SDD103 pKa = 4.33YY104 pKa = 10.82PVNIALPPAEE114 pKa = 5.05LGGLCGTAMCFAGHH128 pKa = 6.33ATFEE132 pKa = 4.5VGDD135 pKa = 3.71QPLFYY140 pKa = 10.62TDD142 pKa = 4.55LNYY145 pKa = 10.59LAHH148 pKa = 6.46NVSDD152 pKa = 3.98DD153 pKa = 3.58GKK155 pKa = 8.47TLEE158 pKa = 4.59LGQVFGDD165 pKa = 3.76LSIHH169 pKa = 5.51QVHH172 pKa = 6.45PVDD175 pKa = 3.72GAKK178 pKa = 10.84GEE180 pKa = 4.21TADD183 pKa = 3.45VGRR186 pKa = 11.84RR187 pKa = 11.84AAEE190 pKa = 3.9LLEE193 pKa = 4.8LDD195 pKa = 4.98SEE197 pKa = 4.58TADD200 pKa = 3.48ILFEE204 pKa = 4.45ASNTLEE210 pKa = 4.49DD211 pKa = 3.88LRR213 pKa = 11.84EE214 pKa = 3.69MVARR218 pKa = 11.84IKK220 pKa = 10.64EE221 pKa = 4.28HH222 pKa = 7.4GGLTWNLKK230 pKa = 10.33CSACDD235 pKa = 3.13VWPWHH240 pKa = 6.53CVDD243 pKa = 4.88EE244 pKa = 6.21GPVCDD249 pKa = 3.15NCGYY253 pKa = 10.74HH254 pKa = 7.24EE255 pKa = 5.17DD256 pKa = 3.74RR257 pKa = 11.84CEE259 pKa = 4.32CEE261 pKa = 5.69DD262 pKa = 5.76DD263 pKa = 4.35IEE265 pKa = 4.61CANCYY270 pKa = 10.19EE271 pKa = 4.29IVAEE275 pKa = 4.84EE276 pKa = 4.68GDD278 pKa = 3.92LCPDD282 pKa = 4.04CEE284 pKa = 4.42RR285 pKa = 11.84KK286 pKa = 9.97EE287 pKa = 4.16STANGGDD294 pKa = 3.67DD295 pKa = 4.22DD296 pKa = 4.36

Molecular weight: 32.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UKK8|A0A2U8UKK8_9CAUD Uncharacterized protein OS=Gordonia phage Sour OX=2182349 GN=73 PE=4 SV=1
MM1 pKa = 7.42LTPTVTRR8 pKa = 11.84GYY10 pKa = 9.8HH11 pKa = 4.11YY12 pKa = 10.81VGMYY16 pKa = 10.2RR17 pKa = 11.84GGSVVRR23 pKa = 11.84ARR25 pKa = 11.84VHH27 pKa = 6.33RR28 pKa = 11.84LVLEE32 pKa = 4.35AFVGPCPEE40 pKa = 4.24GQQACHH46 pKa = 6.91ADD48 pKa = 3.53DD49 pKa = 5.87DD50 pKa = 4.36KK51 pKa = 11.95TNNEE55 pKa = 4.07LTNLRR60 pKa = 11.84WDD62 pKa = 3.8SPSANAADD70 pKa = 3.74MRR72 pKa = 11.84RR73 pKa = 11.84NGRR76 pKa = 11.84NAQARR81 pKa = 11.84KK82 pKa = 7.05TKK84 pKa = 9.93CANGHH89 pKa = 6.67AYY91 pKa = 8.74TPEE94 pKa = 3.85NTYY97 pKa = 10.24VRR99 pKa = 11.84PDD101 pKa = 2.94GRR103 pKa = 11.84GRR105 pKa = 11.84GCKK108 pKa = 9.36RR109 pKa = 11.84CMYY112 pKa = 10.13DD113 pKa = 2.85RR114 pKa = 11.84NKK116 pKa = 10.31AARR119 pKa = 11.84ARR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 3.35

Molecular weight: 13.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

19518

37

1834

247.1

26.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.358 ± 0.281

0.953 ± 0.144

6.742 ± 0.244

6.21 ± 0.332

2.388 ± 0.148

8.464 ± 0.452

2.101 ± 0.2

5.031 ± 0.239

2.413 ± 0.14

8.08 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.15

2.608 ± 0.18

5.492 ± 0.27

3.371 ± 0.184

6.86 ± 0.344

5.984 ± 0.209

6.947 ± 0.244

7.25 ± 0.192

2.034 ± 0.091

2.229 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski