Escherichia phage SRT7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Foetvirus; Escherichia virus SRT7

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5H3D8|A0A2Z5H3D8_9CAUD Uncharacterized protein OS=Escherichia phage SRT7 OX=2268589 PE=4 SV=1
MM1 pKa = 7.03NVEE4 pKa = 4.54TGAEE8 pKa = 4.11LYY10 pKa = 10.5AVNDD14 pKa = 3.29ILAAIGEE21 pKa = 4.59PPVSTLEE28 pKa = 3.98GDD30 pKa = 3.8ANADD34 pKa = 3.17VANARR39 pKa = 11.84RR40 pKa = 11.84VLDD43 pKa = 4.06KK44 pKa = 10.81INRR47 pKa = 11.84QVQSRR52 pKa = 11.84GWTFNIEE59 pKa = 3.84EE60 pKa = 4.76GAVLMPDD67 pKa = 3.72AFSKK71 pKa = 11.02LIVYY75 pKa = 10.42SDD77 pKa = 4.14DD78 pKa = 3.54FLSIFQEE85 pKa = 5.3GSATPYY91 pKa = 10.13VNRR94 pKa = 11.84GGYY97 pKa = 9.96VYY99 pKa = 11.01DD100 pKa = 4.15RR101 pKa = 11.84VNKK104 pKa = 9.22TDD106 pKa = 3.88RR107 pKa = 11.84FDD109 pKa = 3.9NSITVTMIKK118 pKa = 10.36LRR120 pKa = 11.84DD121 pKa = 3.68YY122 pKa = 11.67DD123 pKa = 4.0EE124 pKa = 4.58MPEE127 pKa = 4.1CFRR130 pKa = 11.84TYY132 pKa = 10.45IVTKK136 pKa = 9.43AARR139 pKa = 11.84RR140 pKa = 11.84FNSSFFGAAEE150 pKa = 4.16VEE152 pKa = 4.41AMLADD157 pKa = 3.96EE158 pKa = 4.51EE159 pKa = 4.75LEE161 pKa = 3.97ARR163 pKa = 11.84QMCMEE168 pKa = 4.15YY169 pKa = 10.85EE170 pKa = 3.91MDD172 pKa = 3.6YY173 pKa = 11.37GVYY176 pKa = 11.06NMLDD180 pKa = 3.06GDD182 pKa = 4.34TFVQGQISRR191 pKa = 3.79

Molecular weight: 21.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5H572|A0A2Z5H572_9CAUD Uncharacterized protein OS=Escherichia phage SRT7 OX=2268589 PE=4 SV=1
MM1 pKa = 6.9YY2 pKa = 10.04AAIVALFVRR11 pKa = 11.84DD12 pKa = 3.96FRR14 pKa = 11.84KK15 pKa = 10.56GLIVHH20 pKa = 6.48SANLAYY26 pKa = 9.83FKK28 pKa = 10.25WYY30 pKa = 9.38VLPRR34 pKa = 11.84FTVRR38 pKa = 11.84EE39 pKa = 3.73PNGRR43 pKa = 11.84FKK45 pKa = 11.26ANKK48 pKa = 9.08VGMFYY53 pKa = 10.83VATHH57 pKa = 6.41

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

12449

38

1320

264.9

29.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.752 ± 0.469

0.916 ± 0.137

6.169 ± 0.205

6.924 ± 0.317

3.703 ± 0.209

7.639 ± 0.25

1.807 ± 0.165

4.812 ± 0.199

6.683 ± 0.328

7.985 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.811 ± 0.212

4.659 ± 0.262

3.687 ± 0.168

3.976 ± 0.235

5.358 ± 0.164

5.872 ± 0.37

5.591 ± 0.312

6.739 ± 0.337

1.462 ± 0.176

3.454 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski