Amino acid dipepetide frequency for Escherichia phage SRT7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.889AlaAla: 11.889 ± 1.365
0.884AlaCys: 0.884 ± 0.27
6.186AlaAsp: 6.186 ± 0.528
6.025AlaGlu: 6.025 ± 0.724
3.454AlaPhe: 3.454 ± 0.55
7.712AlaGly: 7.712 ± 0.736
1.125AlaHis: 1.125 ± 0.304
4.981AlaIle: 4.981 ± 0.802
8.114AlaLys: 8.114 ± 0.606
7.873AlaLeu: 7.873 ± 0.896
3.454AlaMet: 3.454 ± 0.606
5.141AlaAsn: 5.141 ± 0.708
2.892AlaPro: 2.892 ± 0.407
3.936AlaGln: 3.936 ± 0.631
4.338AlaArg: 4.338 ± 0.605
5.784AlaSer: 5.784 ± 0.894
4.338AlaThr: 4.338 ± 0.577
5.623AlaVal: 5.623 ± 0.825
1.205AlaTrp: 1.205 ± 0.364
2.571AlaTyr: 2.571 ± 0.386
0.0AlaXaa: 0.0 ± 0.0
Cys
0.964CysAla: 0.964 ± 0.33
0.08CysCys: 0.08 ± 0.084
0.482CysAsp: 0.482 ± 0.199
0.643CysGlu: 0.643 ± 0.256
0.482CysPhe: 0.482 ± 0.237
0.723CysGly: 0.723 ± 0.26
0.321CysHis: 0.321 ± 0.18
0.402CysIle: 0.402 ± 0.21
0.241CysLys: 0.241 ± 0.115
0.402CysLeu: 0.402 ± 0.189
0.161CysMet: 0.161 ± 0.126
0.241CysAsn: 0.241 ± 0.146
0.643CysPro: 0.643 ± 0.232
0.402CysGln: 0.402 ± 0.202
0.803CysArg: 0.803 ± 0.349
0.402CysSer: 0.402 ± 0.206
0.241CysThr: 0.241 ± 0.127
0.723CysVal: 0.723 ± 0.235
0.161CysTrp: 0.161 ± 0.151
0.643CysTyr: 0.643 ± 0.239
0.0CysXaa: 0.0 ± 0.0
Asp
6.507AspAla: 6.507 ± 0.732
0.562AspCys: 0.562 ± 0.193
3.535AspAsp: 3.535 ± 0.702
4.418AspGlu: 4.418 ± 0.459
3.213AspPhe: 3.213 ± 0.522
6.668AspGly: 6.668 ± 0.708
1.285AspHis: 1.285 ± 0.232
2.972AspIle: 2.972 ± 0.487
4.258AspLys: 4.258 ± 0.558
4.017AspLeu: 4.017 ± 0.597
1.928AspMet: 1.928 ± 0.257
2.651AspAsn: 2.651 ± 0.39
2.089AspPro: 2.089 ± 0.562
1.928AspGln: 1.928 ± 0.419
3.053AspArg: 3.053 ± 0.587
2.49AspSer: 2.49 ± 0.464
3.374AspThr: 3.374 ± 0.399
3.776AspVal: 3.776 ± 0.607
0.562AspTrp: 0.562 ± 0.164
2.41AspTyr: 2.41 ± 0.424
0.0AspXaa: 0.0 ± 0.0
Glu
6.748GluAla: 6.748 ± 0.708
1.044GluCys: 1.044 ± 0.305
4.499GluAsp: 4.499 ± 0.416
5.382GluGlu: 5.382 ± 0.99
2.571GluPhe: 2.571 ± 0.454
4.9GluGly: 4.9 ± 0.708
1.607GluHis: 1.607 ± 0.453
2.972GluIle: 2.972 ± 0.349
3.374GluLys: 3.374 ± 0.56
6.025GluLeu: 6.025 ± 0.763
1.607GluMet: 1.607 ± 0.418
2.892GluAsn: 2.892 ± 0.572
2.169GluPro: 2.169 ± 0.489
2.731GluGln: 2.731 ± 0.735
4.258GluArg: 4.258 ± 0.505
4.177GluSer: 4.177 ± 0.52
3.856GluThr: 3.856 ± 0.419
4.338GluVal: 4.338 ± 0.483
0.803GluTrp: 0.803 ± 0.262
3.294GluTyr: 3.294 ± 0.35
0.0GluXaa: 0.0 ± 0.0
Phe
3.695PheAla: 3.695 ± 0.648
0.723PheCys: 0.723 ± 0.272
2.49PheAsp: 2.49 ± 0.514
2.33PheGlu: 2.33 ± 0.383
0.803PhePhe: 0.803 ± 0.284
2.571PheGly: 2.571 ± 0.415
0.803PheHis: 0.803 ± 0.21
1.687PheIle: 1.687 ± 0.439
2.49PheLys: 2.49 ± 0.579
3.053PheLeu: 3.053 ± 0.525
1.285PheMet: 1.285 ± 0.303
1.928PheAsn: 1.928 ± 0.418
2.008PhePro: 2.008 ± 0.347
0.964PheGln: 0.964 ± 0.302
1.687PheArg: 1.687 ± 0.323
1.928PheSer: 1.928 ± 0.33
2.892PheThr: 2.892 ± 0.394
2.089PheVal: 2.089 ± 0.287
0.241PheTrp: 0.241 ± 0.137
1.366PheTyr: 1.366 ± 0.25
0.0PheXaa: 0.0 ± 0.0
Gly
6.828GlyAla: 6.828 ± 0.854
0.884GlyCys: 0.884 ± 0.267
4.579GlyAsp: 4.579 ± 0.763
4.659GlyGlu: 4.659 ± 0.606
3.213GlyPhe: 3.213 ± 0.423
5.704GlyGly: 5.704 ± 0.697
0.964GlyHis: 0.964 ± 0.264
3.695GlyIle: 3.695 ± 0.461
5.463GlyLys: 5.463 ± 0.629
7.15GlyLeu: 7.15 ± 1.049
1.687GlyMet: 1.687 ± 0.427
2.892GlyAsn: 2.892 ± 0.396
1.366GlyPro: 1.366 ± 0.458
3.454GlyGln: 3.454 ± 0.363
4.74GlyArg: 4.74 ± 0.497
6.105GlySer: 6.105 ± 0.725
3.936GlyThr: 3.936 ± 0.479
4.82GlyVal: 4.82 ± 0.637
1.446GlyTrp: 1.446 ± 0.492
2.812GlyTyr: 2.812 ± 0.371
0.0GlyXaa: 0.0 ± 0.0
His
1.366HisAla: 1.366 ± 0.378
0.241HisCys: 0.241 ± 0.14
1.687HisAsp: 1.687 ± 0.458
1.366HisGlu: 1.366 ± 0.367
0.964HisPhe: 0.964 ± 0.222
1.366HisGly: 1.366 ± 0.276
0.723HisHis: 0.723 ± 0.241
0.964HisIle: 0.964 ± 0.233
1.125HisLys: 1.125 ± 0.355
1.767HisLeu: 1.767 ± 0.374
0.643HisMet: 0.643 ± 0.182
1.044HisAsn: 1.044 ± 0.275
0.402HisPro: 0.402 ± 0.185
0.161HisGln: 0.161 ± 0.143
0.643HisArg: 0.643 ± 0.239
0.723HisSer: 0.723 ± 0.21
0.803HisThr: 0.803 ± 0.257
0.803HisVal: 0.803 ± 0.277
0.562HisTrp: 0.562 ± 0.206
0.723HisTyr: 0.723 ± 0.207
0.0HisXaa: 0.0 ± 0.0
Ile
4.097IleAla: 4.097 ± 0.481
0.723IleCys: 0.723 ± 0.231
2.972IleAsp: 2.972 ± 0.551
3.936IleGlu: 3.936 ± 0.579
1.044IlePhe: 1.044 ± 0.294
3.133IleGly: 3.133 ± 0.492
1.366IleHis: 1.366 ± 0.334
1.687IleIle: 1.687 ± 0.361
2.731IleLys: 2.731 ± 0.389
3.294IleLeu: 3.294 ± 0.574
1.607IleMet: 1.607 ± 0.338
2.169IleAsn: 2.169 ± 0.455
2.812IlePro: 2.812 ± 0.436
2.089IleGln: 2.089 ± 0.334
4.177IleArg: 4.177 ± 0.645
2.008IleSer: 2.008 ± 0.341
2.571IleThr: 2.571 ± 0.333
3.294IleVal: 3.294 ± 0.467
0.643IleTrp: 0.643 ± 0.245
1.767IleTyr: 1.767 ± 0.411
0.0IleXaa: 0.0 ± 0.0
Lys
8.033LysAla: 8.033 ± 1.145
0.321LysCys: 0.321 ± 0.164
3.374LysAsp: 3.374 ± 0.582
3.856LysGlu: 3.856 ± 0.531
2.41LysPhe: 2.41 ± 0.371
4.338LysGly: 4.338 ± 0.544
1.446LysHis: 1.446 ± 0.393
2.41LysIle: 2.41 ± 0.439
3.615LysLys: 3.615 ± 0.534
6.668LysLeu: 6.668 ± 0.706
2.008LysMet: 2.008 ± 0.472
1.607LysAsn: 1.607 ± 0.27
3.535LysPro: 3.535 ± 0.625
2.651LysGln: 2.651 ± 0.439
3.294LysArg: 3.294 ± 0.654
4.82LysSer: 4.82 ± 0.591
3.615LysThr: 3.615 ± 0.425
4.9LysVal: 4.9 ± 0.558
1.687LysTrp: 1.687 ± 0.367
2.249LysTyr: 2.249 ± 0.32
0.0LysXaa: 0.0 ± 0.0
Leu
7.632LeuAla: 7.632 ± 0.875
0.321LeuCys: 0.321 ± 0.159
5.061LeuAsp: 5.061 ± 0.6
6.668LeuGlu: 6.668 ± 0.833
2.571LeuPhe: 2.571 ± 0.56
5.382LeuGly: 5.382 ± 0.765
1.446LeuHis: 1.446 ± 0.293
3.776LeuIle: 3.776 ± 0.547
6.105LeuLys: 6.105 ± 0.672
5.864LeuLeu: 5.864 ± 0.65
2.33LeuMet: 2.33 ± 0.322
4.017LeuAsn: 4.017 ± 0.542
3.294LeuPro: 3.294 ± 0.484
3.133LeuGln: 3.133 ± 0.39
5.061LeuArg: 5.061 ± 0.635
4.258LeuSer: 4.258 ± 0.634
4.418LeuThr: 4.418 ± 0.662
4.659LeuVal: 4.659 ± 0.626
1.687LeuTrp: 1.687 ± 0.319
2.169LeuTyr: 2.169 ± 0.367
0.0LeuXaa: 0.0 ± 0.0
Met
3.454MetAla: 3.454 ± 0.418
0.161MetCys: 0.161 ± 0.116
1.366MetAsp: 1.366 ± 0.279
1.607MetGlu: 1.607 ± 0.392
1.285MetPhe: 1.285 ± 0.306
2.41MetGly: 2.41 ± 0.777
0.402MetHis: 0.402 ± 0.144
1.687MetIle: 1.687 ± 0.351
1.607MetLys: 1.607 ± 0.259
2.008MetLeu: 2.008 ± 0.373
0.562MetMet: 0.562 ± 0.218
1.607MetAsn: 1.607 ± 0.312
1.285MetPro: 1.285 ± 0.306
1.044MetGln: 1.044 ± 0.282
1.044MetArg: 1.044 ± 0.267
1.928MetSer: 1.928 ± 0.401
1.607MetThr: 1.607 ± 0.324
2.008MetVal: 2.008 ± 0.467
0.0MetTrp: 0.0 ± 0.0
1.044MetTyr: 1.044 ± 0.274
0.0MetXaa: 0.0 ± 0.0
Asn
4.097AsnAla: 4.097 ± 0.615
0.562AsnCys: 0.562 ± 0.263
2.892AsnAsp: 2.892 ± 0.584
2.571AsnGlu: 2.571 ± 0.396
1.366AsnPhe: 1.366 ± 0.282
4.9AsnGly: 4.9 ± 0.646
0.321AsnHis: 0.321 ± 0.168
2.249AsnIle: 2.249 ± 0.364
3.776AsnLys: 3.776 ± 0.545
4.097AsnLeu: 4.097 ± 0.507
1.205AsnMet: 1.205 ± 0.335
1.607AsnAsn: 1.607 ± 0.378
3.053AsnPro: 3.053 ± 0.521
1.446AsnGln: 1.446 ± 0.273
2.731AsnArg: 2.731 ± 0.512
2.169AsnSer: 2.169 ± 0.555
2.169AsnThr: 2.169 ± 0.411
3.454AsnVal: 3.454 ± 0.488
0.402AsnTrp: 0.402 ± 0.151
1.526AsnTyr: 1.526 ± 0.356
0.0AsnXaa: 0.0 ± 0.0
Pro
3.454ProAla: 3.454 ± 0.427
0.482ProCys: 0.482 ± 0.248
2.812ProAsp: 2.812 ± 0.419
4.177ProGlu: 4.177 ± 0.621
1.446ProPhe: 1.446 ± 0.369
2.089ProGly: 2.089 ± 0.421
0.321ProHis: 0.321 ± 0.127
1.526ProIle: 1.526 ± 0.243
2.249ProLys: 2.249 ± 0.357
1.767ProLeu: 1.767 ± 0.226
0.884ProMet: 0.884 ± 0.287
2.41ProAsn: 2.41 ± 0.448
0.562ProPro: 0.562 ± 0.184
1.366ProGln: 1.366 ± 0.343
1.607ProArg: 1.607 ± 0.43
2.169ProSer: 2.169 ± 0.308
2.169ProThr: 2.169 ± 0.414
3.133ProVal: 3.133 ± 0.465
0.402ProTrp: 0.402 ± 0.158
1.848ProTyr: 1.848 ± 0.542
0.0ProXaa: 0.0 ± 0.0
Gln
4.097GlnAla: 4.097 ± 0.469
0.161GlnCys: 0.161 ± 0.117
2.33GlnAsp: 2.33 ± 0.407
2.651GlnGlu: 2.651 ± 0.527
2.651GlnPhe: 2.651 ± 0.324
2.249GlnGly: 2.249 ± 0.443
0.562GlnHis: 0.562 ± 0.21
1.687GlnIle: 1.687 ± 0.364
2.089GlnLys: 2.089 ± 0.345
3.133GlnLeu: 3.133 ± 0.462
1.044GlnMet: 1.044 ± 0.27
1.366GlnAsn: 1.366 ± 0.402
1.767GlnPro: 1.767 ± 0.253
2.41GlnGln: 2.41 ± 0.465
2.249GlnArg: 2.249 ± 0.564
2.41GlnSer: 2.41 ± 0.427
2.008GlnThr: 2.008 ± 0.401
2.892GlnVal: 2.892 ± 0.53
0.482GlnTrp: 0.482 ± 0.168
1.526GlnTyr: 1.526 ± 0.31
0.0GlnXaa: 0.0 ± 0.0
Arg
5.222ArgAla: 5.222 ± 0.838
0.643ArgCys: 0.643 ± 0.215
4.097ArgAsp: 4.097 ± 0.602
3.936ArgGlu: 3.936 ± 0.794
1.526ArgPhe: 1.526 ± 0.475
3.695ArgGly: 3.695 ± 0.57
1.125ArgHis: 1.125 ± 0.318
2.731ArgIle: 2.731 ± 0.522
4.177ArgLys: 4.177 ± 0.705
4.74ArgLeu: 4.74 ± 0.539
1.848ArgMet: 1.848 ± 0.31
2.812ArgAsn: 2.812 ± 0.333
1.285ArgPro: 1.285 ± 0.286
2.33ArgGln: 2.33 ± 0.395
2.249ArgArg: 2.249 ± 0.449
2.651ArgSer: 2.651 ± 0.59
2.49ArgThr: 2.49 ± 0.557
3.856ArgVal: 3.856 ± 0.567
0.643ArgTrp: 0.643 ± 0.204
1.526ArgTyr: 1.526 ± 0.362
0.0ArgXaa: 0.0 ± 0.0
Ser
5.141SerAla: 5.141 ± 0.908
0.643SerCys: 0.643 ± 0.212
3.695SerAsp: 3.695 ± 0.572
2.731SerGlu: 2.731 ± 0.609
2.33SerPhe: 2.33 ± 0.376
5.382SerGly: 5.382 ± 0.571
1.205SerHis: 1.205 ± 0.337
3.776SerIle: 3.776 ± 0.557
3.615SerLys: 3.615 ± 0.558
5.061SerLeu: 5.061 ± 0.567
1.446SerMet: 1.446 ± 0.378
2.892SerAsn: 2.892 ± 0.46
1.607SerPro: 1.607 ± 0.382
2.49SerGln: 2.49 ± 0.458
2.812SerArg: 2.812 ± 0.462
3.776SerSer: 3.776 ± 0.645
3.294SerThr: 3.294 ± 0.623
3.695SerVal: 3.695 ± 0.505
1.125SerTrp: 1.125 ± 0.317
2.008SerTyr: 2.008 ± 0.348
0.0SerXaa: 0.0 ± 0.0
Thr
4.579ThrAla: 4.579 ± 0.595
0.321ThrCys: 0.321 ± 0.24
2.892ThrAsp: 2.892 ± 0.364
3.535ThrGlu: 3.535 ± 0.503
2.249ThrPhe: 2.249 ± 0.419
5.141ThrGly: 5.141 ± 0.761
0.723ThrHis: 0.723 ± 0.225
3.454ThrIle: 3.454 ± 0.617
3.936ThrLys: 3.936 ± 0.423
4.097ThrLeu: 4.097 ± 0.646
1.366ThrMet: 1.366 ± 0.369
2.972ThrAsn: 2.972 ± 0.666
2.249ThrPro: 2.249 ± 0.442
2.41ThrGln: 2.41 ± 0.478
2.169ThrArg: 2.169 ± 0.411
3.615ThrSer: 3.615 ± 0.791
4.097ThrThr: 4.097 ± 0.526
4.177ThrVal: 4.177 ± 0.488
0.562ThrTrp: 0.562 ± 0.187
1.366ThrTyr: 1.366 ± 0.267
0.0ThrXaa: 0.0 ± 0.0
Val
5.864ValAla: 5.864 ± 0.792
0.161ValCys: 0.161 ± 0.114
3.133ValAsp: 3.133 ± 0.413
5.222ValGlu: 5.222 ± 0.633
2.089ValPhe: 2.089 ± 0.465
4.579ValGly: 4.579 ± 0.654
1.446ValHis: 1.446 ± 0.413
3.615ValIle: 3.615 ± 0.568
4.981ValLys: 4.981 ± 0.688
4.9ValLeu: 4.9 ± 0.709
1.446ValMet: 1.446 ± 0.301
3.454ValAsn: 3.454 ± 0.771
2.41ValPro: 2.41 ± 0.408
2.651ValGln: 2.651 ± 0.445
3.374ValArg: 3.374 ± 0.532
5.302ValSer: 5.302 ± 0.588
4.177ValThr: 4.177 ± 0.548
4.74ValVal: 4.74 ± 0.856
1.205ValTrp: 1.205 ± 0.34
2.651ValTyr: 2.651 ± 0.572
0.0ValXaa: 0.0 ± 0.0
Trp
0.803TrpAla: 0.803 ± 0.258
0.08TrpCys: 0.08 ± 0.084
0.482TrpAsp: 0.482 ± 0.189
1.285TrpGlu: 1.285 ± 0.312
0.562TrpPhe: 0.562 ± 0.244
0.643TrpGly: 0.643 ± 0.193
0.402TrpHis: 0.402 ± 0.178
0.402TrpIle: 0.402 ± 0.176
1.205TrpLys: 1.205 ± 0.338
2.008TrpLeu: 2.008 ± 0.414
0.402TrpMet: 0.402 ± 0.14
0.964TrpAsn: 0.964 ± 0.279
0.321TrpPro: 0.321 ± 0.152
0.562TrpGln: 0.562 ± 0.186
0.803TrpArg: 0.803 ± 0.197
0.964TrpSer: 0.964 ± 0.325
0.723TrpThr: 0.723 ± 0.234
1.446TrpVal: 1.446 ± 0.45
0.402TrpTrp: 0.402 ± 0.158
0.161TrpTyr: 0.161 ± 0.134
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.053TyrAla: 3.053 ± 0.449
0.161TyrCys: 0.161 ± 0.108
3.133TyrAsp: 3.133 ± 0.511
2.249TyrGlu: 2.249 ± 0.423
0.803TyrPhe: 0.803 ± 0.288
2.812TyrGly: 2.812 ± 0.533
0.482TyrHis: 0.482 ± 0.252
1.446TyrIle: 1.446 ± 0.512
1.687TyrLys: 1.687 ± 0.233
1.928TyrLeu: 1.928 ± 0.457
1.125TyrMet: 1.125 ± 0.328
1.928TyrAsn: 1.928 ± 0.287
1.125TyrPro: 1.125 ± 0.297
1.607TyrGln: 1.607 ± 0.317
2.49TyrArg: 2.49 ± 0.435
1.044TyrSer: 1.044 ± 0.274
3.133TyrThr: 3.133 ± 0.569
2.972TyrVal: 2.972 ± 0.502
0.402TyrTrp: 0.402 ± 0.23
0.884TyrTyr: 0.884 ± 0.266
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (12449 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski