Slackia equolifaciens
Average proteome isoelectric point is 5.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2149 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N0AR58|A0A3N0AR58_9ACTN Protein kinase domain-containing protein OS=Slackia equolifaciens OX=498718 GN=DMP06_10985 PE=4 SV=1
MM1 pKa = 7.26 VPTMLNQRR9 pKa = 11.84 SASRR13 pKa = 11.84 GRR15 pKa = 11.84 SRR17 pKa = 11.84 GRR19 pKa = 11.84 VLKK22 pKa = 9.5 QQPQGMKK29 pKa = 7.49 RR30 pKa = 11.84 TRR32 pKa = 11.84 RR33 pKa = 11.84 AFVALLAAVVLAFGVGSAAYY53 pKa = 9.54 ANPIDD58 pKa = 4.38 DD59 pKa = 3.68 VVGAVTSFFTGSSNDD74 pKa = 3.35 EE75 pKa = 4.02 SSDD78 pKa = 3.63 AGVSTQAVEE87 pKa = 4.69 DD88 pKa = 3.92 YY89 pKa = 9.84 TVTGVSPRR97 pKa = 11.84 GTTINVFDD105 pKa = 3.93 YY106 pKa = 10.5 WITSQDD112 pKa = 3.47 DD113 pKa = 3.83 PDD115 pKa = 4.25 NVTQALVNDD124 pKa = 3.56 QGEE127 pKa = 4.2 LNEE130 pKa = 4.89 RR131 pKa = 11.84 YY132 pKa = 9.53 KK133 pKa = 10.89 QGINADD139 pKa = 3.47 HH140 pKa = 6.28 TLKK143 pKa = 10.83 FGAGMGTSTDD153 pKa = 3.54 NNEE156 pKa = 3.93 DD157 pKa = 3.92 LINRR161 pKa = 11.84 RR162 pKa = 11.84 DD163 pKa = 3.38 INYY166 pKa = 6.86 WTGDD170 pKa = 3.4 SAPRR174 pKa = 11.84 QGIVSDD180 pKa = 4.22 TLTDD184 pKa = 4.78 GYY186 pKa = 8.8 PTLSNAGGIGSEE198 pKa = 3.99 SLAYY202 pKa = 10.83 LFDD205 pKa = 4.28 NSTQEE210 pKa = 3.75 GKK212 pKa = 9.97 AAYY215 pKa = 9.02 MDD217 pKa = 3.71 VDD219 pKa = 3.81 GLLQVDD225 pKa = 3.82 DD226 pKa = 3.65 QGYY229 pKa = 9.93 YY230 pKa = 10.55 YY231 pKa = 11.15 YY232 pKa = 11.18 NSQDD236 pKa = 2.93 NFAQFNADD244 pKa = 3.19 GSGSGNFTLYY254 pKa = 10.67 NRR256 pKa = 11.84 WGVEE260 pKa = 4.11 AAGSSPDD267 pKa = 3.43 GQFFPFNTGDD277 pKa = 3.65 EE278 pKa = 4.5 VFDD281 pKa = 3.92 EE282 pKa = 4.43 QNGQLVEE289 pKa = 4.2 QQVNTSQGRR298 pKa = 11.84 RR299 pKa = 11.84 DD300 pKa = 4.24 LISTDD305 pKa = 3.33 EE306 pKa = 4.45 VINHH310 pKa = 5.45 YY311 pKa = 10.51 FGLNMSTRR319 pKa = 11.84 FIQQNGGYY327 pKa = 6.38 TAPDD331 pKa = 3.71 GTQGRR336 pKa = 11.84 QQVTYY341 pKa = 10.33 NFSGDD346 pKa = 3.73 DD347 pKa = 3.96 DD348 pKa = 3.79 VWIYY352 pKa = 10.79 IDD354 pKa = 3.94 GVLVGDD360 pKa = 4.85 LGGIHH365 pKa = 7.62 DD366 pKa = 4.39 ATSIEE371 pKa = 4.29 INFATGRR378 pKa = 11.84 VYY380 pKa = 10.26 IYY382 pKa = 10.9 DD383 pKa = 4.7 DD384 pKa = 3.72 GTFGDD389 pKa = 3.86 NDD391 pKa = 3.44 SRR393 pKa = 11.84 SNNNEE398 pKa = 3.36 YY399 pKa = 10.92 DD400 pKa = 3.51 QGEE403 pKa = 4.3 TLYY406 pKa = 11.06 NGSNGQTIAEE416 pKa = 4.26 IMRR419 pKa = 11.84 AAGVTTGLTDD429 pKa = 3.11 TTFADD434 pKa = 4.06 DD435 pKa = 4.15 TYY437 pKa = 10.33 HH438 pKa = 6.4 TLDD441 pKa = 3.45 FFYY444 pKa = 11.1 LEE446 pKa = 4.71 RR447 pKa = 11.84 GNTDD451 pKa = 2.73 SNMSLKK457 pKa = 10.67 YY458 pKa = 10.24 NLVNIPEE465 pKa = 4.16 SGIVKK470 pKa = 9.48 VDD472 pKa = 3.29 QYY474 pKa = 11.69 GDD476 pKa = 3.48 EE477 pKa = 4.11 LAGVQFTLSEE487 pKa = 4.2 ANSNYY492 pKa = 9.54 EE493 pKa = 4.29 AADD496 pKa = 3.65 NNNDD500 pKa = 3.35 GVVTEE505 pKa = 4.09 VSGRR509 pKa = 11.84 TDD511 pKa = 3.18 SNGEE515 pKa = 3.95 MIFTYY520 pKa = 10.29 EE521 pKa = 4.06 NNSGQEE527 pKa = 3.81 MPITLEE533 pKa = 3.86 QMGEE537 pKa = 3.86 RR538 pKa = 11.84 SAYY541 pKa = 8.97 WILEE545 pKa = 3.96 EE546 pKa = 5.37 AEE548 pKa = 4.19 GNEE551 pKa = 4.31 TIGYY555 pKa = 7.52 RR556 pKa = 11.84 NPGTVEE562 pKa = 3.97 LRR564 pKa = 11.84 FSTINGDD571 pKa = 3.57 GTGDD575 pKa = 3.65 GVLLSSNQWEE585 pKa = 4.3 TGAYY589 pKa = 8.82 SQAHH593 pKa = 4.65 VTATAPTNVTAVDD606 pKa = 3.23 NGGTYY611 pKa = 10.31 EE612 pKa = 4.23 SAGSLMFAVVAQIDD626 pKa = 3.92 EE627 pKa = 4.25 QGNYY631 pKa = 9.59 HH632 pKa = 6.98 AVTGDD637 pKa = 3.75 AFAGWSVADD646 pKa = 3.86 ATIDD650 pKa = 3.85 DD651 pKa = 4.5 ASTSKK656 pKa = 10.95 DD657 pKa = 3.63 AIVTAAKK664 pKa = 10.38 ASGYY668 pKa = 10.23 QFLLGSGGAYY678 pKa = 9.3 QVTIDD683 pKa = 5.15 DD684 pKa = 4.67 LPGDD688 pKa = 3.92 INTYY692 pKa = 10.35 EE693 pKa = 3.96 YY694 pKa = 10.23 MIEE697 pKa = 4.03 QAGGDD702 pKa = 3.69 TSTAQYY708 pKa = 9.77 AVKK711 pKa = 10.35 YY712 pKa = 8.38 YY713 pKa = 10.04 WSDD716 pKa = 2.99 ATSIDD721 pKa = 4.05 DD722 pKa = 4.64 LSSNSQIVEE731 pKa = 3.83 IDD733 pKa = 3.67 PNTGDD738 pKa = 3.08 GFEE741 pKa = 5.26 RR742 pKa = 11.84 IFSVTLSIPNIKK754 pKa = 10.44 NEE756 pKa = 3.77 LSLVKK761 pKa = 9.89 TDD763 pKa = 3.83 EE764 pKa = 4.42 TTGDD768 pKa = 4.18 PIAGVVFNMYY778 pKa = 9.83 EE779 pKa = 4.15 DD780 pKa = 4.0 KK781 pKa = 11.53 NGDD784 pKa = 3.04 GTYY787 pKa = 11.15 NNGEE791 pKa = 4.18 RR792 pKa = 11.84 CCFTGTTGTDD802 pKa = 2.93 GGLEE806 pKa = 3.67 ITSNINNGEE815 pKa = 3.95 QVLAKK820 pKa = 10.42 GEE822 pKa = 4.21 YY823 pKa = 9.8 VLVEE827 pKa = 4.37 TVPSGYY833 pKa = 10.15 VDD835 pKa = 3.64 EE836 pKa = 4.39 QTSIRR841 pKa = 11.84 VIVDD845 pKa = 3.17 DD846 pKa = 5.68 DD847 pKa = 4.35 GVHH850 pKa = 6.51 VDD852 pKa = 5.5 AGDD855 pKa = 3.92 ANDD858 pKa = 3.57 NVTVEE863 pKa = 4.18 TGIGSLVYY871 pKa = 10.77 SMRR874 pKa = 11.84 GFAAGDD880 pKa = 3.82 DD881 pKa = 4.13 VDD883 pKa = 4.38 STLHH887 pKa = 6.81 DD888 pKa = 3.73 VVAQAQTADD897 pKa = 3.76 SYY899 pKa = 11.57 EE900 pKa = 4.36 GASTAWDD907 pKa = 3.31 NADD910 pKa = 3.73 AVTHH914 pKa = 5.31 YY915 pKa = 10.73 QYY917 pKa = 11.62 DD918 pKa = 3.88 DD919 pKa = 3.34 QTDD922 pKa = 3.56 GLNYY926 pKa = 9.53 IRR928 pKa = 11.84 NDD930 pKa = 3.13 NVTDD934 pKa = 3.63 NGDD937 pKa = 3.23 ATFIADD943 pKa = 4.13 AGWSRR948 pKa = 11.84 LDD950 pKa = 3.82 LRR952 pKa = 11.84 QCTAHH957 pKa = 6.94 AGEE960 pKa = 5.15 DD961 pKa = 3.87 PIEE964 pKa = 4.31 NKK966 pKa = 9.99 QNLGDD971 pKa = 3.61 QSLNALFTGDD981 pKa = 3.44 VTIHH985 pKa = 5.19 VTNSQIPTTSSLTISKK1001 pKa = 8.63 TVTGTGAPSDD1011 pKa = 3.7 VTFDD1015 pKa = 3.79 FSFSLTDD1022 pKa = 3.24 AGGNSVTGTFSATTHH1037 pKa = 6.49 RR1038 pKa = 11.84 ADD1040 pKa = 3.78 GTEE1043 pKa = 3.9 STADD1047 pKa = 3.19 MVIYY1051 pKa = 10.89 AEE1053 pKa = 4.53 ADD1055 pKa = 3.7 CNQLTLRR1062 pKa = 11.84 NGEE1065 pKa = 4.26 SVTIDD1070 pKa = 3.66 GLPISANYY1078 pKa = 8.31 TVTEE1082 pKa = 4.08 EE1083 pKa = 4.0 AKK1085 pKa = 10.48 GYY1087 pKa = 7.06 YY1088 pKa = 8.6 TPSVQQSSGTLNEE1101 pKa = 5.1 DD1102 pKa = 3.53 GSFTATGTIDD1112 pKa = 3.83 NDD1114 pKa = 3.61 GATAAFSNDD1123 pKa = 2.67 FWGGSVDD1130 pKa = 3.59 YY1131 pKa = 10.6 DD1132 pKa = 3.63 ASIDD1136 pKa = 3.79 LTVSKK1141 pKa = 11.13 VMTGRR1146 pKa = 11.84 DD1147 pKa = 3.17 MTAGEE1152 pKa = 4.31 FAVVVDD1158 pKa = 5.2 PINDD1162 pKa = 3.2 EE1163 pKa = 4.19 SAALLGLANASEE1175 pKa = 4.36 NKK1177 pKa = 9.86 VIDD1180 pKa = 3.67 MPAANDD1186 pKa = 3.99 GVEE1189 pKa = 4.06 VSVDD1193 pKa = 3.11 ILNGLNDD1200 pKa = 3.42 IAFTAEE1206 pKa = 4.12 DD1207 pKa = 3.69 ADD1209 pKa = 3.78 KK1210 pKa = 10.47 TFTYY1214 pKa = 9.41 TVYY1217 pKa = 10.42 EE1218 pKa = 4.16 QQSDD1222 pKa = 3.87 QNPSNGVTYY1231 pKa = 8.54 DD1232 pKa = 3.3 TARR1235 pKa = 11.84 YY1236 pKa = 9.24 SLTIEE1241 pKa = 4.13 VSVDD1245 pKa = 2.72 ADD1247 pKa = 3.53 GVVTVHH1253 pKa = 5.92 TAVSNEE1259 pKa = 3.77 TGTLRR1264 pKa = 11.84 EE1265 pKa = 4.12 MTTTPNSGGTMPVAVFNNSYY1285 pKa = 10.7 NATGEE1290 pKa = 4.08 LGGNGSASIKK1300 pKa = 8.36 ATKK1303 pKa = 10.29 VLEE1306 pKa = 4.22 NDD1308 pKa = 3.25 TLAAGDD1314 pKa = 3.65 FTFNVAAVNSNGTDD1328 pKa = 3.22 EE1329 pKa = 4.91 RR1330 pKa = 11.84 VEE1332 pKa = 3.97 ATTTNGTNGAASAADD1347 pKa = 3.23 IVFPAIQYY1355 pKa = 10.85 DD1356 pKa = 4.18 SDD1358 pKa = 4.14 SLAQDD1363 pKa = 3.27 VADD1366 pKa = 5.08 GYY1368 pKa = 11.86 ARR1370 pKa = 11.84 ARR1372 pKa = 11.84 VNDD1375 pKa = 3.81 NGTTTYY1381 pKa = 9.59 TYY1383 pKa = 10.64 LYY1385 pKa = 9.8 RR1386 pKa = 11.84 VYY1388 pKa = 10.46 EE1389 pKa = 4.18 DD1390 pKa = 3.23 TSGFDD1395 pKa = 3.7 GLGLTTSQSSYY1406 pKa = 11.23 NVTVWVTDD1414 pKa = 3.97 DD1415 pKa = 3.9 GDD1417 pKa = 3.74 GSLEE1421 pKa = 4.16 VEE1423 pKa = 4.14 VGYY1426 pKa = 8.42 PTGSEE1431 pKa = 3.73 NGITFTNVYY1440 pKa = 9.25 GASANATLNVGGTKK1454 pKa = 9.23 IYY1456 pKa = 7.57 EE1457 pKa = 4.28 TNGLKK1462 pKa = 10.09 NAPSIEE1468 pKa = 3.93 GKK1470 pKa = 8.55 YY1471 pKa = 8.48 TFTLTGVDD1479 pKa = 3.55 EE1480 pKa = 5.5 DD1481 pKa = 4.81 GNAAPLPGGATGSVTAQNQANGTVDD1506 pKa = 4.64 FGTITYY1512 pKa = 10.06 DD1513 pKa = 3.19 IADD1516 pKa = 4.3 LGGADD1521 pKa = 4.14 SKK1523 pKa = 10.28 TFTYY1527 pKa = 10.24 TVTEE1531 pKa = 4.05 SGGVDD1536 pKa = 3.25 GVTNDD1541 pKa = 3.67 GAASTGKK1548 pKa = 8.16 TFTVTLTDD1556 pKa = 3.44 NGKK1559 pKa = 8.4 GTISVDD1565 pKa = 3.22 SDD1567 pKa = 3.45 ALQAAGSQFSFTNEE1581 pKa = 3.76 YY1582 pKa = 10.85 SVDD1585 pKa = 3.64 PTDD1588 pKa = 4.04 PTDD1591 pKa = 3.79 PTDD1594 pKa = 4.24 PGTDD1598 pKa = 3.3 PSDD1601 pKa = 3.53 PTTAALSISKK1611 pKa = 10.04 VLTGRR1616 pKa = 11.84 AMNEE1620 pKa = 4.1 GEE1622 pKa = 4.85 FSFTMAAAGDD1632 pKa = 3.88 YY1633 pKa = 10.62 GNAVSPTSITATNMVNEE1650 pKa = 5.15 DD1651 pKa = 3.32 GTGSVVFGDD1660 pKa = 3.45 GFVFTEE1666 pKa = 4.03 PGTYY1670 pKa = 10.17 HH1671 pKa = 5.47 FTISEE1676 pKa = 4.24 DD1677 pKa = 3.34 VGTLGGVSYY1686 pKa = 10.99 DD1687 pKa = 3.39 GSDD1690 pKa = 3.55 YY1691 pKa = 10.86 TATAVVTDD1699 pKa = 4.91 DD1700 pKa = 3.6 GAGNLSVAWTVADD1713 pKa = 3.66 AEE1715 pKa = 4.59 GNAVSEE1721 pKa = 4.23 ITFEE1725 pKa = 4.03 NTYY1728 pKa = 7.14 TTSATTPVEE1737 pKa = 4.0 LKK1739 pKa = 10.57 VSKK1742 pKa = 10.05 EE1743 pKa = 4.07 LQDD1746 pKa = 3.32 GTLADD1751 pKa = 3.88 GQFTFEE1757 pKa = 4.39 LTGSDD1762 pKa = 4.07 GAPMPEE1768 pKa = 4.2 STTVTNNAQGVVSFGSITFDD1788 pKa = 3.34 AVGEE1792 pKa = 3.95 YY1793 pKa = 10.02 DD1794 pKa = 3.75 YY1795 pKa = 11.56 TVTEE1799 pKa = 4.4 VNDD1802 pKa = 4.27 GQDD1805 pKa = 3.42 GITYY1809 pKa = 10.53 DD1810 pKa = 3.81 EE1811 pKa = 4.89 DD1812 pKa = 3.41 ATRR1815 pKa = 11.84 TIHH1818 pKa = 7.16 VSVTDD1823 pKa = 3.88 DD1824 pKa = 4.2 GEE1826 pKa = 4.3 GHH1828 pKa = 6.93 LVAEE1832 pKa = 4.49 VTYY1835 pKa = 10.7 GSDD1838 pKa = 2.98 GSHH1841 pKa = 6.04 FVNVNTTGGNNDD1853 pKa = 3.74 GGDD1856 pKa = 3.63 NGDD1859 pKa = 4.23 GGNGGDD1865 pKa = 4.16 NGGTGDD1871 pKa = 4.32 AGNGSGNGSSFAQTNDD1887 pKa = 2.93 ATPWPLAMALALVAAGAIIAAVFGLRR1913 pKa = 11.84 RR1914 pKa = 11.84 AQRR1917 pKa = 3.33
Molecular weight: 201.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.678
IPC2_protein 3.681
IPC_protein 3.745
Toseland 3.503
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.401
Solomon 3.732
Lehninger 3.694
Nozaki 3.834
DTASelect 4.113
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.859
Patrickios 1.189
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.758
Protein with the highest isoelectric point:
>tr|A0A3N0AUU4|A0A3N0AUU4_9ACTN LysR family transcriptional regulator OS=Slackia equolifaciens OX=498718 GN=DMP06_09035 PE=3 SV=1
MM1 pKa = 7.39 AAAPRR6 pKa = 11.84 YY7 pKa = 6.09 VTCLFGSMKK16 pKa = 10.22 SGKK19 pKa = 8.17 FTQRR23 pKa = 11.84 STEE26 pKa = 3.7 AKK28 pKa = 8.83 AARR31 pKa = 11.84 GTFVRR36 pKa = 11.84 WCAEE40 pKa = 3.51 NNVRR44 pKa = 11.84 HH45 pKa = 5.74 VSDD48 pKa = 4.31 FQHH51 pKa = 6.7 FDD53 pKa = 2.75 IGGYY57 pKa = 8.65 QYY59 pKa = 11.66 NEE61 pKa = 4.1 ALSSEE66 pKa = 4.16 DD67 pKa = 3.24 TFVFTHH73 pKa = 5.6 QGRR76 pKa = 11.84 PLRR79 pKa = 11.84 CPMGAAIVRR88 pKa = 11.84 LGSVSGAAQEE98 pKa = 4.02 ASPRR102 pKa = 11.84 KK103 pKa = 9.55 ARR105 pKa = 11.84 SGDD108 pKa = 3.16 SRR110 pKa = 11.84 RR111 pKa = 11.84 LRR113 pKa = 11.84 RR114 pKa = 11.84 PII116 pKa = 3.62
Molecular weight: 12.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.531
IPC_protein 10.379
Toseland 10.452
ProMoST 10.204
Dawson 10.599
Bjellqvist 10.35
Wikipedia 10.818
Rodwell 10.716
Grimsley 10.657
Solomon 10.701
Lehninger 10.672
Nozaki 10.496
DTASelect 10.321
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.54
Patrickios 10.467
IPC_peptide 10.701
IPC2_peptide 9.604
IPC2.peptide.svr19 8.5
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2149
0
2149
742730
36
3987
345.6
37.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.816 ± 0.075
1.667 ± 0.028
6.081 ± 0.038
6.757 ± 0.052
3.847 ± 0.032
8.316 ± 0.058
1.861 ± 0.023
5.253 ± 0.042
3.785 ± 0.041
8.784 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.821 ± 0.025
2.934 ± 0.038
4.253 ± 0.037
2.981 ± 0.03
5.849 ± 0.061
6.104 ± 0.049
5.099 ± 0.064
7.865 ± 0.046
1.055 ± 0.019
2.871 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here