Slackia equolifaciens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Slackia

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2149 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3N0AR58|A0A3N0AR58_9ACTN Protein kinase domain-containing protein OS=Slackia equolifaciens OX=498718 GN=DMP06_10985 PE=4 SV=1
MM1 pKa = 7.26VPTMLNQRR9 pKa = 11.84SASRR13 pKa = 11.84GRR15 pKa = 11.84SRR17 pKa = 11.84GRR19 pKa = 11.84VLKK22 pKa = 9.5QQPQGMKK29 pKa = 7.49RR30 pKa = 11.84TRR32 pKa = 11.84RR33 pKa = 11.84AFVALLAAVVLAFGVGSAAYY53 pKa = 9.54ANPIDD58 pKa = 4.38DD59 pKa = 3.68VVGAVTSFFTGSSNDD74 pKa = 3.35EE75 pKa = 4.02SSDD78 pKa = 3.63AGVSTQAVEE87 pKa = 4.69DD88 pKa = 3.92YY89 pKa = 9.84TVTGVSPRR97 pKa = 11.84GTTINVFDD105 pKa = 3.93YY106 pKa = 10.5WITSQDD112 pKa = 3.47DD113 pKa = 3.83PDD115 pKa = 4.25NVTQALVNDD124 pKa = 3.56QGEE127 pKa = 4.2LNEE130 pKa = 4.89RR131 pKa = 11.84YY132 pKa = 9.53KK133 pKa = 10.89QGINADD139 pKa = 3.47HH140 pKa = 6.28TLKK143 pKa = 10.83FGAGMGTSTDD153 pKa = 3.54NNEE156 pKa = 3.93DD157 pKa = 3.92LINRR161 pKa = 11.84RR162 pKa = 11.84DD163 pKa = 3.38INYY166 pKa = 6.86WTGDD170 pKa = 3.4SAPRR174 pKa = 11.84QGIVSDD180 pKa = 4.22TLTDD184 pKa = 4.78GYY186 pKa = 8.8PTLSNAGGIGSEE198 pKa = 3.99SLAYY202 pKa = 10.83LFDD205 pKa = 4.28NSTQEE210 pKa = 3.75GKK212 pKa = 9.97AAYY215 pKa = 9.02MDD217 pKa = 3.71VDD219 pKa = 3.81GLLQVDD225 pKa = 3.82DD226 pKa = 3.65QGYY229 pKa = 9.93YY230 pKa = 10.55YY231 pKa = 11.15YY232 pKa = 11.18NSQDD236 pKa = 2.93NFAQFNADD244 pKa = 3.19GSGSGNFTLYY254 pKa = 10.67NRR256 pKa = 11.84WGVEE260 pKa = 4.11AAGSSPDD267 pKa = 3.43GQFFPFNTGDD277 pKa = 3.65EE278 pKa = 4.5VFDD281 pKa = 3.92EE282 pKa = 4.43QNGQLVEE289 pKa = 4.2QQVNTSQGRR298 pKa = 11.84RR299 pKa = 11.84DD300 pKa = 4.24LISTDD305 pKa = 3.33EE306 pKa = 4.45VINHH310 pKa = 5.45YY311 pKa = 10.51FGLNMSTRR319 pKa = 11.84FIQQNGGYY327 pKa = 6.38TAPDD331 pKa = 3.71GTQGRR336 pKa = 11.84QQVTYY341 pKa = 10.33NFSGDD346 pKa = 3.73DD347 pKa = 3.96DD348 pKa = 3.79VWIYY352 pKa = 10.79IDD354 pKa = 3.94GVLVGDD360 pKa = 4.85LGGIHH365 pKa = 7.62DD366 pKa = 4.39ATSIEE371 pKa = 4.29INFATGRR378 pKa = 11.84VYY380 pKa = 10.26IYY382 pKa = 10.9DD383 pKa = 4.7DD384 pKa = 3.72GTFGDD389 pKa = 3.86NDD391 pKa = 3.44SRR393 pKa = 11.84SNNNEE398 pKa = 3.36YY399 pKa = 10.92DD400 pKa = 3.51QGEE403 pKa = 4.3TLYY406 pKa = 11.06NGSNGQTIAEE416 pKa = 4.26IMRR419 pKa = 11.84AAGVTTGLTDD429 pKa = 3.11TTFADD434 pKa = 4.06DD435 pKa = 4.15TYY437 pKa = 10.33HH438 pKa = 6.4TLDD441 pKa = 3.45FFYY444 pKa = 11.1LEE446 pKa = 4.71RR447 pKa = 11.84GNTDD451 pKa = 2.73SNMSLKK457 pKa = 10.67YY458 pKa = 10.24NLVNIPEE465 pKa = 4.16SGIVKK470 pKa = 9.48VDD472 pKa = 3.29QYY474 pKa = 11.69GDD476 pKa = 3.48EE477 pKa = 4.11LAGVQFTLSEE487 pKa = 4.2ANSNYY492 pKa = 9.54EE493 pKa = 4.29AADD496 pKa = 3.65NNNDD500 pKa = 3.35GVVTEE505 pKa = 4.09VSGRR509 pKa = 11.84TDD511 pKa = 3.18SNGEE515 pKa = 3.95MIFTYY520 pKa = 10.29EE521 pKa = 4.06NNSGQEE527 pKa = 3.81MPITLEE533 pKa = 3.86QMGEE537 pKa = 3.86RR538 pKa = 11.84SAYY541 pKa = 8.97WILEE545 pKa = 3.96EE546 pKa = 5.37AEE548 pKa = 4.19GNEE551 pKa = 4.31TIGYY555 pKa = 7.52RR556 pKa = 11.84NPGTVEE562 pKa = 3.97LRR564 pKa = 11.84FSTINGDD571 pKa = 3.57GTGDD575 pKa = 3.65GVLLSSNQWEE585 pKa = 4.3TGAYY589 pKa = 8.82SQAHH593 pKa = 4.65VTATAPTNVTAVDD606 pKa = 3.23NGGTYY611 pKa = 10.31EE612 pKa = 4.23SAGSLMFAVVAQIDD626 pKa = 3.92EE627 pKa = 4.25QGNYY631 pKa = 9.59HH632 pKa = 6.98AVTGDD637 pKa = 3.75AFAGWSVADD646 pKa = 3.86ATIDD650 pKa = 3.85DD651 pKa = 4.5ASTSKK656 pKa = 10.95DD657 pKa = 3.63AIVTAAKK664 pKa = 10.38ASGYY668 pKa = 10.23QFLLGSGGAYY678 pKa = 9.3QVTIDD683 pKa = 5.15DD684 pKa = 4.67LPGDD688 pKa = 3.92INTYY692 pKa = 10.35EE693 pKa = 3.96YY694 pKa = 10.23MIEE697 pKa = 4.03QAGGDD702 pKa = 3.69TSTAQYY708 pKa = 9.77AVKK711 pKa = 10.35YY712 pKa = 8.38YY713 pKa = 10.04WSDD716 pKa = 2.99ATSIDD721 pKa = 4.05DD722 pKa = 4.64LSSNSQIVEE731 pKa = 3.83IDD733 pKa = 3.67PNTGDD738 pKa = 3.08GFEE741 pKa = 5.26RR742 pKa = 11.84IFSVTLSIPNIKK754 pKa = 10.44NEE756 pKa = 3.77LSLVKK761 pKa = 9.89TDD763 pKa = 3.83EE764 pKa = 4.42TTGDD768 pKa = 4.18PIAGVVFNMYY778 pKa = 9.83EE779 pKa = 4.15DD780 pKa = 4.0KK781 pKa = 11.53NGDD784 pKa = 3.04GTYY787 pKa = 11.15NNGEE791 pKa = 4.18RR792 pKa = 11.84CCFTGTTGTDD802 pKa = 2.93GGLEE806 pKa = 3.67ITSNINNGEE815 pKa = 3.95QVLAKK820 pKa = 10.42GEE822 pKa = 4.21YY823 pKa = 9.8VLVEE827 pKa = 4.37TVPSGYY833 pKa = 10.15VDD835 pKa = 3.64EE836 pKa = 4.39QTSIRR841 pKa = 11.84VIVDD845 pKa = 3.17DD846 pKa = 5.68DD847 pKa = 4.35GVHH850 pKa = 6.51VDD852 pKa = 5.5AGDD855 pKa = 3.92ANDD858 pKa = 3.57NVTVEE863 pKa = 4.18TGIGSLVYY871 pKa = 10.77SMRR874 pKa = 11.84GFAAGDD880 pKa = 3.82DD881 pKa = 4.13VDD883 pKa = 4.38STLHH887 pKa = 6.81DD888 pKa = 3.73VVAQAQTADD897 pKa = 3.76SYY899 pKa = 11.57EE900 pKa = 4.36GASTAWDD907 pKa = 3.31NADD910 pKa = 3.73AVTHH914 pKa = 5.31YY915 pKa = 10.73QYY917 pKa = 11.62DD918 pKa = 3.88DD919 pKa = 3.34QTDD922 pKa = 3.56GLNYY926 pKa = 9.53IRR928 pKa = 11.84NDD930 pKa = 3.13NVTDD934 pKa = 3.63NGDD937 pKa = 3.23ATFIADD943 pKa = 4.13AGWSRR948 pKa = 11.84LDD950 pKa = 3.82LRR952 pKa = 11.84QCTAHH957 pKa = 6.94AGEE960 pKa = 5.15DD961 pKa = 3.87PIEE964 pKa = 4.31NKK966 pKa = 9.99QNLGDD971 pKa = 3.61QSLNALFTGDD981 pKa = 3.44VTIHH985 pKa = 5.19VTNSQIPTTSSLTISKK1001 pKa = 8.63TVTGTGAPSDD1011 pKa = 3.7VTFDD1015 pKa = 3.79FSFSLTDD1022 pKa = 3.24AGGNSVTGTFSATTHH1037 pKa = 6.49RR1038 pKa = 11.84ADD1040 pKa = 3.78GTEE1043 pKa = 3.9STADD1047 pKa = 3.19MVIYY1051 pKa = 10.89AEE1053 pKa = 4.53ADD1055 pKa = 3.7CNQLTLRR1062 pKa = 11.84NGEE1065 pKa = 4.26SVTIDD1070 pKa = 3.66GLPISANYY1078 pKa = 8.31TVTEE1082 pKa = 4.08EE1083 pKa = 4.0AKK1085 pKa = 10.48GYY1087 pKa = 7.06YY1088 pKa = 8.6TPSVQQSSGTLNEE1101 pKa = 5.1DD1102 pKa = 3.53GSFTATGTIDD1112 pKa = 3.83NDD1114 pKa = 3.61GATAAFSNDD1123 pKa = 2.67FWGGSVDD1130 pKa = 3.59YY1131 pKa = 10.6DD1132 pKa = 3.63ASIDD1136 pKa = 3.79LTVSKK1141 pKa = 11.13VMTGRR1146 pKa = 11.84DD1147 pKa = 3.17MTAGEE1152 pKa = 4.31FAVVVDD1158 pKa = 5.2PINDD1162 pKa = 3.2EE1163 pKa = 4.19SAALLGLANASEE1175 pKa = 4.36NKK1177 pKa = 9.86VIDD1180 pKa = 3.67MPAANDD1186 pKa = 3.99GVEE1189 pKa = 4.06VSVDD1193 pKa = 3.11ILNGLNDD1200 pKa = 3.42IAFTAEE1206 pKa = 4.12DD1207 pKa = 3.69ADD1209 pKa = 3.78KK1210 pKa = 10.47TFTYY1214 pKa = 9.41TVYY1217 pKa = 10.42EE1218 pKa = 4.16QQSDD1222 pKa = 3.87QNPSNGVTYY1231 pKa = 8.54DD1232 pKa = 3.3TARR1235 pKa = 11.84YY1236 pKa = 9.24SLTIEE1241 pKa = 4.13VSVDD1245 pKa = 2.72ADD1247 pKa = 3.53GVVTVHH1253 pKa = 5.92TAVSNEE1259 pKa = 3.77TGTLRR1264 pKa = 11.84EE1265 pKa = 4.12MTTTPNSGGTMPVAVFNNSYY1285 pKa = 10.7NATGEE1290 pKa = 4.08LGGNGSASIKK1300 pKa = 8.36ATKK1303 pKa = 10.29VLEE1306 pKa = 4.22NDD1308 pKa = 3.25TLAAGDD1314 pKa = 3.65FTFNVAAVNSNGTDD1328 pKa = 3.22EE1329 pKa = 4.91RR1330 pKa = 11.84VEE1332 pKa = 3.97ATTTNGTNGAASAADD1347 pKa = 3.23IVFPAIQYY1355 pKa = 10.85DD1356 pKa = 4.18SDD1358 pKa = 4.14SLAQDD1363 pKa = 3.27VADD1366 pKa = 5.08GYY1368 pKa = 11.86ARR1370 pKa = 11.84ARR1372 pKa = 11.84VNDD1375 pKa = 3.81NGTTTYY1381 pKa = 9.59TYY1383 pKa = 10.64LYY1385 pKa = 9.8RR1386 pKa = 11.84VYY1388 pKa = 10.46EE1389 pKa = 4.18DD1390 pKa = 3.23TSGFDD1395 pKa = 3.7GLGLTTSQSSYY1406 pKa = 11.23NVTVWVTDD1414 pKa = 3.97DD1415 pKa = 3.9GDD1417 pKa = 3.74GSLEE1421 pKa = 4.16VEE1423 pKa = 4.14VGYY1426 pKa = 8.42PTGSEE1431 pKa = 3.73NGITFTNVYY1440 pKa = 9.25GASANATLNVGGTKK1454 pKa = 9.23IYY1456 pKa = 7.57EE1457 pKa = 4.28TNGLKK1462 pKa = 10.09NAPSIEE1468 pKa = 3.93GKK1470 pKa = 8.55YY1471 pKa = 8.48TFTLTGVDD1479 pKa = 3.55EE1480 pKa = 5.5DD1481 pKa = 4.81GNAAPLPGGATGSVTAQNQANGTVDD1506 pKa = 4.64FGTITYY1512 pKa = 10.06DD1513 pKa = 3.19IADD1516 pKa = 4.3LGGADD1521 pKa = 4.14SKK1523 pKa = 10.28TFTYY1527 pKa = 10.24TVTEE1531 pKa = 4.05SGGVDD1536 pKa = 3.25GVTNDD1541 pKa = 3.67GAASTGKK1548 pKa = 8.16TFTVTLTDD1556 pKa = 3.44NGKK1559 pKa = 8.4GTISVDD1565 pKa = 3.22SDD1567 pKa = 3.45ALQAAGSQFSFTNEE1581 pKa = 3.76YY1582 pKa = 10.85SVDD1585 pKa = 3.64PTDD1588 pKa = 4.04PTDD1591 pKa = 3.79PTDD1594 pKa = 4.24PGTDD1598 pKa = 3.3PSDD1601 pKa = 3.53PTTAALSISKK1611 pKa = 10.04VLTGRR1616 pKa = 11.84AMNEE1620 pKa = 4.1GEE1622 pKa = 4.85FSFTMAAAGDD1632 pKa = 3.88YY1633 pKa = 10.62GNAVSPTSITATNMVNEE1650 pKa = 5.15DD1651 pKa = 3.32GTGSVVFGDD1660 pKa = 3.45GFVFTEE1666 pKa = 4.03PGTYY1670 pKa = 10.17HH1671 pKa = 5.47FTISEE1676 pKa = 4.24DD1677 pKa = 3.34VGTLGGVSYY1686 pKa = 10.99DD1687 pKa = 3.39GSDD1690 pKa = 3.55YY1691 pKa = 10.86TATAVVTDD1699 pKa = 4.91DD1700 pKa = 3.6GAGNLSVAWTVADD1713 pKa = 3.66AEE1715 pKa = 4.59GNAVSEE1721 pKa = 4.23ITFEE1725 pKa = 4.03NTYY1728 pKa = 7.14TTSATTPVEE1737 pKa = 4.0LKK1739 pKa = 10.57VSKK1742 pKa = 10.05EE1743 pKa = 4.07LQDD1746 pKa = 3.32GTLADD1751 pKa = 3.88GQFTFEE1757 pKa = 4.39LTGSDD1762 pKa = 4.07GAPMPEE1768 pKa = 4.2STTVTNNAQGVVSFGSITFDD1788 pKa = 3.34AVGEE1792 pKa = 3.95YY1793 pKa = 10.02DD1794 pKa = 3.75YY1795 pKa = 11.56TVTEE1799 pKa = 4.4VNDD1802 pKa = 4.27GQDD1805 pKa = 3.42GITYY1809 pKa = 10.53DD1810 pKa = 3.81EE1811 pKa = 4.89DD1812 pKa = 3.41ATRR1815 pKa = 11.84TIHH1818 pKa = 7.16VSVTDD1823 pKa = 3.88DD1824 pKa = 4.2GEE1826 pKa = 4.3GHH1828 pKa = 6.93LVAEE1832 pKa = 4.49VTYY1835 pKa = 10.7GSDD1838 pKa = 2.98GSHH1841 pKa = 6.04FVNVNTTGGNNDD1853 pKa = 3.74GGDD1856 pKa = 3.63NGDD1859 pKa = 4.23GGNGGDD1865 pKa = 4.16NGGTGDD1871 pKa = 4.32AGNGSGNGSSFAQTNDD1887 pKa = 2.93ATPWPLAMALALVAAGAIIAAVFGLRR1913 pKa = 11.84RR1914 pKa = 11.84AQRR1917 pKa = 3.33

Molecular weight:
201.65 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3N0AUU4|A0A3N0AUU4_9ACTN LysR family transcriptional regulator OS=Slackia equolifaciens OX=498718 GN=DMP06_09035 PE=3 SV=1
MM1 pKa = 7.39AAAPRR6 pKa = 11.84YY7 pKa = 6.09VTCLFGSMKK16 pKa = 10.22SGKK19 pKa = 8.17FTQRR23 pKa = 11.84STEE26 pKa = 3.7AKK28 pKa = 8.83AARR31 pKa = 11.84GTFVRR36 pKa = 11.84WCAEE40 pKa = 3.51NNVRR44 pKa = 11.84HH45 pKa = 5.74VSDD48 pKa = 4.31FQHH51 pKa = 6.7FDD53 pKa = 2.75IGGYY57 pKa = 8.65QYY59 pKa = 11.66NEE61 pKa = 4.1ALSSEE66 pKa = 4.16DD67 pKa = 3.24TFVFTHH73 pKa = 5.6QGRR76 pKa = 11.84PLRR79 pKa = 11.84CPMGAAIVRR88 pKa = 11.84LGSVSGAAQEE98 pKa = 4.02ASPRR102 pKa = 11.84KK103 pKa = 9.55ARR105 pKa = 11.84SGDD108 pKa = 3.16SRR110 pKa = 11.84RR111 pKa = 11.84LRR113 pKa = 11.84RR114 pKa = 11.84PII116 pKa = 3.62

Molecular weight:
12.84 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2149

0

2149

742730

36

3987

345.6

37.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.816 ± 0.075

1.667 ± 0.028

6.081 ± 0.038

6.757 ± 0.052

3.847 ± 0.032

8.316 ± 0.058

1.861 ± 0.023

5.253 ± 0.042

3.785 ± 0.041

8.784 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.821 ± 0.025

2.934 ± 0.038

4.253 ± 0.037

2.981 ± 0.03

5.849 ± 0.061

6.104 ± 0.049

5.099 ± 0.064

7.865 ± 0.046

1.055 ± 0.019

2.871 ± 0.025

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski