Sulfitobacter sp. JBTF-M27

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Sulfitobacter

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4488 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P0C9J0|A0A6P0C9J0_9RHOB Peptidoglycan DD-metalloendopeptidase family protein OS=Sulfitobacter sp. JBTF-M27 OX=2698830 GN=GV827_08940 PE=4 SV=1
MM1 pKa = 7.22FPEE4 pKa = 5.11DD5 pKa = 4.13SPLLAALASYY15 pKa = 10.94GSDD18 pKa = 2.69EE19 pKa = 4.37RR20 pKa = 11.84LYY22 pKa = 10.48TSTVTFRR29 pKa = 11.84FMPAGSEE36 pKa = 4.16IDD38 pKa = 4.81DD39 pKa = 4.6GDD41 pKa = 4.39PNTPNEE47 pKa = 4.14VSVGWSEE54 pKa = 5.03PNMQYY59 pKa = 10.77VRR61 pKa = 11.84GTFDD65 pKa = 3.81YY66 pKa = 10.42ISSVVDD72 pKa = 3.16LTFAEE77 pKa = 4.85TTSSDD82 pKa = 3.16ATISFYY88 pKa = 11.08EE89 pKa = 4.06VAEE92 pKa = 4.39FSDD95 pKa = 3.81DD96 pKa = 3.21TAGYY100 pKa = 8.23VTFISEE106 pKa = 4.81GISIAVIGSQFTGPVAFDD124 pKa = 3.46NDD126 pKa = 3.8YY127 pKa = 8.61TTIIHH132 pKa = 6.87EE133 pKa = 4.42IGHH136 pKa = 7.07AIGLSHH142 pKa = 6.99PHH144 pKa = 7.3DD145 pKa = 4.97GPAVLPGVTSQDD157 pKa = 3.44DD158 pKa = 3.8KK159 pKa = 12.09GDD161 pKa = 4.08LNLNTEE167 pKa = 4.33FTTRR171 pKa = 11.84MSYY174 pKa = 10.24IPGEE178 pKa = 4.41SPSNPGVDD186 pKa = 2.54IWGEE190 pKa = 4.0ANTFGAIDD198 pKa = 3.54IAALQLLYY206 pKa = 10.74GANTQTGLGDD216 pKa = 3.6TVYY219 pKa = 11.33GDD221 pKa = 3.43TRR223 pKa = 11.84DD224 pKa = 5.3LVTIWDD230 pKa = 3.53NGGFDD235 pKa = 5.18RR236 pKa = 11.84IDD238 pKa = 3.5FSSATEE244 pKa = 3.93DD245 pKa = 3.43AVIDD249 pKa = 3.85LRR251 pKa = 11.84AATLQFEE258 pKa = 4.75VGGGGYY264 pKa = 10.48LSYY267 pKa = 10.64ISSRR271 pKa = 11.84NGTVADD277 pKa = 3.61GGYY280 pKa = 10.23AIAYY284 pKa = 8.49GVEE287 pKa = 3.98IEE289 pKa = 4.23NAMGGAGADD298 pKa = 3.27MLMGNALANTFVGGGGNDD316 pKa = 3.56VIDD319 pKa = 4.65GGDD322 pKa = 3.56GRR324 pKa = 11.84DD325 pKa = 3.19TGAYY329 pKa = 9.71SGNQSSYY336 pKa = 10.58TLTLSAQTTTLADD349 pKa = 3.52RR350 pKa = 11.84RR351 pKa = 11.84GTDD354 pKa = 3.32GTDD357 pKa = 2.86TLVNMEE363 pKa = 5.26LLTFGDD369 pKa = 3.71AAVAPFDD376 pKa = 3.68LTVFAGTTGLSEE388 pKa = 4.14TQMEE392 pKa = 4.67SFIEE396 pKa = 4.25LYY398 pKa = 9.75IAYY401 pKa = 9.93FNRR404 pKa = 11.84APDD407 pKa = 3.65AVGLNFWGTAFASGTTLEE425 pKa = 4.12QMATLFTDD433 pKa = 3.29QDD435 pKa = 3.79EE436 pKa = 4.62TRR438 pKa = 11.84ATYY441 pKa = 10.48GPEE444 pKa = 3.67LSNADD449 pKa = 3.41FATAVYY455 pKa = 10.26GNVLGRR461 pKa = 11.84VADD464 pKa = 3.61QAGFDD469 pKa = 3.67FWVGVLDD476 pKa = 3.54SGARR480 pKa = 11.84TRR482 pKa = 11.84DD483 pKa = 3.39QFILSVLEE491 pKa = 4.02GAKK494 pKa = 10.05AAPFEE499 pKa = 4.31GAPADD504 pKa = 4.48FVAQQIADD512 pKa = 3.99RR513 pKa = 11.84QYY515 pKa = 11.37LSTKK519 pKa = 8.83TDD521 pKa = 2.5IGAYY525 pKa = 7.99YY526 pKa = 10.69AVIRR530 pKa = 11.84GMSDD534 pKa = 2.95VTNATAAMNAFDD546 pKa = 5.09GSAAGINAAVAAIDD560 pKa = 4.91GYY562 pKa = 10.82FQAAQDD568 pKa = 3.59GDD570 pKa = 3.77TGQFLMPLVGVLDD583 pKa = 4.26DD584 pKa = 4.73PFAGMVV590 pKa = 3.18

Molecular weight: 62.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P0CFE8|A0A6P0CFE8_9RHOB MBL fold metallo-hydrolase OS=Sulfitobacter sp. JBTF-M27 OX=2698830 GN=GV827_21190 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.81IINARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.34SLSAA44 pKa = 3.83

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4488

0

4488

1404947

34

3221

313.0

34.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.728 ± 0.046

0.901 ± 0.012

6.258 ± 0.036

6.009 ± 0.035

3.823 ± 0.024

8.41 ± 0.04

2.063 ± 0.02

5.449 ± 0.027

3.596 ± 0.035

9.816 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.894 ± 0.019

2.835 ± 0.02

4.91 ± 0.026

3.353 ± 0.02

6.234 ± 0.033

5.324 ± 0.023

5.553 ± 0.027

7.18 ± 0.031

1.382 ± 0.013

2.281 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski