Beihai tombus-like virus 13

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFV4|A0A1L3KFV4_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 13 OX=1922716 PE=4 SV=1
MM1 pKa = 7.7VPICRR6 pKa = 11.84GVQHH10 pKa = 7.08LLEE13 pKa = 4.31NHH15 pKa = 6.4PQDD18 pKa = 4.32EE19 pKa = 4.49NASPDD24 pKa = 3.69LLLDD28 pKa = 3.42TRR30 pKa = 11.84NLGFHH35 pKa = 6.09RR36 pKa = 11.84QSYY39 pKa = 9.64HH40 pKa = 5.79YY41 pKa = 10.58KK42 pKa = 10.55LFNLPPSMEE51 pKa = 4.28CMLEE55 pKa = 3.83KK56 pKa = 9.83QTFYY60 pKa = 11.59NNCYY64 pKa = 9.13CNEE67 pKa = 3.98YY68 pKa = 9.9NAMIGRR74 pKa = 11.84HH75 pKa = 5.26FTPDD79 pKa = 3.48LPEE82 pKa = 4.7KK83 pKa = 10.76SSIPALTEE91 pKa = 3.68LFEE94 pKa = 4.56KK95 pKa = 10.8EE96 pKa = 3.9NIKK99 pKa = 11.01YY100 pKa = 9.43CDD102 pKa = 3.35SMKK105 pKa = 10.75EE106 pKa = 4.03LLFSTGGYY114 pKa = 9.56NPPSYY119 pKa = 11.16ASVISNTKK127 pKa = 8.28GAKK130 pKa = 8.64KK131 pKa = 9.99KK132 pKa = 10.16RR133 pKa = 11.84YY134 pKa = 8.43QRR136 pKa = 11.84AYY138 pKa = 11.02DD139 pKa = 3.84NILNRR144 pKa = 11.84RR145 pKa = 11.84ILFDD149 pKa = 3.49NRR151 pKa = 11.84HH152 pKa = 5.05SRR154 pKa = 11.84VNFFVKK160 pKa = 10.51LEE162 pKa = 3.8KK163 pKa = 10.21WPISKK168 pKa = 10.03VEE170 pKa = 4.02KK171 pKa = 9.8EE172 pKa = 4.0KK173 pKa = 10.71PPRR176 pKa = 11.84GIQFRR181 pKa = 11.84SYY183 pKa = 11.02EE184 pKa = 3.89YY185 pKa = 11.32LLALKK190 pKa = 10.4RR191 pKa = 11.84LITPMVDD198 pKa = 3.04LTKK201 pKa = 10.15TNKK204 pKa = 8.87TFEE207 pKa = 4.65HH208 pKa = 6.36NPPFNPQCVFTKK220 pKa = 10.84NNTPQVIAANFLEE233 pKa = 4.58AWQKK237 pKa = 10.99YY238 pKa = 5.62KK239 pKa = 10.8TPTAVCLDD247 pKa = 3.82HH248 pKa = 7.36SCFDD252 pKa = 3.24GHH254 pKa = 6.48YY255 pKa = 9.36SHH257 pKa = 7.22EE258 pKa = 4.13LLTYY262 pKa = 10.27EE263 pKa = 3.88MQRR266 pKa = 11.84YY267 pKa = 7.54QRR269 pKa = 11.84ISGCKK274 pKa = 9.26NNSLLMRR281 pKa = 11.84LLKK284 pKa = 10.54RR285 pKa = 11.84QLTNTGYY292 pKa = 9.91SAGGIKK298 pKa = 10.57YY299 pKa = 8.98KK300 pKa = 11.04VKK302 pKa = 10.47GKK304 pKa = 9.69RR305 pKa = 11.84CSGEE309 pKa = 3.71FTTSHH314 pKa = 6.49GNGEE318 pKa = 4.39TNYY321 pKa = 11.24LMIRR325 pKa = 11.84SILLFLGIMEE335 pKa = 4.56FNIFVNGDD343 pKa = 3.5DD344 pKa = 5.02SVIICNFEE352 pKa = 3.95DD353 pKa = 3.17VDD355 pKa = 4.94RR356 pKa = 11.84IVKK359 pKa = 10.03NLEE362 pKa = 3.56LFRR365 pKa = 11.84NFNMSTEE372 pKa = 4.25VEE374 pKa = 4.56HH375 pKa = 6.62IATVFEE381 pKa = 5.27EE382 pKa = 4.07ITFCQTSPVLVNGTYY397 pKa = 11.06KK398 pKa = 9.82MVRR401 pKa = 11.84KK402 pKa = 9.41PMRR405 pKa = 11.84VLSRR409 pKa = 11.84LPYY412 pKa = 10.63SSTNWASVLDD422 pKa = 3.89RR423 pKa = 11.84FMVSVGLCEE432 pKa = 3.99LSINIGVPILQEE444 pKa = 3.6LAIWLIRR451 pKa = 11.84KK452 pKa = 8.84GGSDD456 pKa = 4.33RR457 pKa = 11.84PLTTHH462 pKa = 6.27KK463 pKa = 9.32TDD465 pKa = 3.64HH466 pKa = 6.73YY467 pKa = 11.01DD468 pKa = 3.17AQAVLKK474 pKa = 10.43IEE476 pKa = 5.41NIDD479 pKa = 3.31QSTRR483 pKa = 11.84EE484 pKa = 4.15SFNLAFGIDD493 pKa = 3.43PHH495 pKa = 6.02EE496 pKa = 4.01QLRR499 pKa = 11.84LEE501 pKa = 4.38KK502 pKa = 10.53YY503 pKa = 10.69LRR505 pKa = 11.84DD506 pKa = 4.62DD507 pKa = 4.02YY508 pKa = 11.37CDD510 pKa = 4.39HH511 pKa = 7.88PDD513 pKa = 3.96QIQTLLTKK521 pKa = 10.76LNYY524 pKa = 10.08KK525 pKa = 10.45LYY527 pKa = 10.94

Molecular weight: 61.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFV4|A0A1L3KFV4_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 13 OX=1922716 PE=4 SV=1
MM1 pKa = 7.51EE2 pKa = 4.05MAFYY6 pKa = 10.19QIRR9 pKa = 11.84EE10 pKa = 4.18MPPLLARR17 pKa = 11.84IEE19 pKa = 4.12EE20 pKa = 4.34RR21 pKa = 11.84PQRR24 pKa = 11.84RR25 pKa = 11.84AEE27 pKa = 3.98DD28 pKa = 3.81RR29 pKa = 11.84PVLSRR34 pKa = 11.84MFYY37 pKa = 10.71KK38 pKa = 10.4LRR40 pKa = 11.84KK41 pKa = 8.73IKK43 pKa = 10.79NHH45 pKa = 5.19MKK47 pKa = 10.0KK48 pKa = 10.56APAEE52 pKa = 3.96EE53 pKa = 4.64HH54 pKa = 6.66NILPVLQEE62 pKa = 4.2TITPVALHH70 pKa = 6.06NAFSLLHH77 pKa = 6.47DD78 pKa = 5.11AGDD81 pKa = 3.62IVNDD85 pKa = 3.66EE86 pKa = 4.43QIDD89 pKa = 3.83IVLSEE94 pKa = 4.34TPKK97 pKa = 10.44KK98 pKa = 10.73VKK100 pKa = 10.44DD101 pKa = 3.94VIKK104 pKa = 10.37KK105 pKa = 9.58VPKK108 pKa = 9.37AQLNARR114 pKa = 11.84LATVDD119 pKa = 4.04EE120 pKa = 4.63NGLLQTSANFVEE132 pKa = 4.57HH133 pKa = 7.25DD134 pKa = 3.64VAIEE138 pKa = 3.85DD139 pKa = 3.96RR140 pKa = 11.84FEE142 pKa = 3.96MSTDD146 pKa = 3.36RR147 pKa = 11.84EE148 pKa = 4.2RR149 pKa = 11.84IRR151 pKa = 11.84PAKK154 pKa = 9.71RR155 pKa = 11.84IKK157 pKa = 10.83GEE159 pKa = 3.65FRR161 pKa = 11.84KK162 pKa = 10.22AVAAVVKK169 pKa = 10.59SDD171 pKa = 2.91MYY173 pKa = 11.3YY174 pKa = 10.62YY175 pKa = 10.28MKK177 pKa = 10.13IKK179 pKa = 10.76YY180 pKa = 9.24FMKK183 pKa = 10.04EE184 pKa = 3.69RR185 pKa = 11.84TPGMVQQIINDD196 pKa = 3.4CRR198 pKa = 11.84VYY200 pKa = 9.64LTKK203 pKa = 10.59SGSLMNTKK211 pKa = 10.05EE212 pKa = 4.16DD213 pKa = 3.97YY214 pKa = 10.78EE215 pKa = 4.45LVSSLVLAIWLPSRR229 pKa = 11.84EE230 pKa = 3.97EE231 pKa = 3.77LRR233 pKa = 11.84FRR235 pKa = 11.84SAMKK239 pKa = 10.78NNVVLDD245 pKa = 4.5GIEE248 pKa = 5.64AINQFNKK255 pKa = 10.44GNLGNRR261 pKa = 11.84FSIPIPSVSRR271 pKa = 11.84PSSLTKK277 pKa = 10.3QVTINPKK284 pKa = 8.47QQ285 pKa = 3.09

Molecular weight: 32.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1266

285

527

422.0

47.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.687 ± 1.086

1.343 ± 0.621

4.265 ± 0.323

5.134 ± 1.281

4.344 ± 0.375

4.66 ± 0.65

1.896 ± 0.597

6.951 ± 0.526

6.319 ± 0.873

8.768 ± 0.957

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.449 ± 0.652

6.872 ± 0.367

5.766 ± 0.479

4.028 ± 0.262

5.134 ± 1.047

7.899 ± 1.331

7.82 ± 1.971

5.687 ± 0.597

0.79 ± 0.156

4.186 ± 0.44
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski