Pseudomonas phage nickie

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nickievirus; Pseudomonas virus nickie

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 161 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4P744|A0A2H4P744_9CAUD Uncharacterized protein OS=Pseudomonas phage nickie OX=2048977 GN=CNR34_00071 PE=4 SV=1
MM1 pKa = 6.73SQKK4 pKa = 10.58VYY6 pKa = 11.13LFTADD11 pKa = 4.44LGDD14 pKa = 3.84GSSTVRR20 pKa = 11.84FTRR23 pKa = 11.84DD24 pKa = 2.74YY25 pKa = 11.82DD26 pKa = 4.38LLDD29 pKa = 3.96KK30 pKa = 11.12LCEE33 pKa = 4.25EE34 pKa = 5.13DD35 pKa = 3.67PEE37 pKa = 4.33QYY39 pKa = 11.26GMNEE43 pKa = 4.42GYY45 pKa = 10.47SRR47 pKa = 11.84TLTFPDD53 pKa = 3.93DD54 pKa = 4.16VNLSEE59 pKa = 5.28CGFNFYY65 pKa = 10.98EE66 pKa = 4.88DD67 pKa = 3.28

Molecular weight: 7.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4P741|A0A2H4P741_9CAUD Uncharacterized protein OS=Pseudomonas phage nickie OX=2048977 GN=CNR34_00058 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.58LSLDD6 pKa = 3.42KK7 pKa = 10.65MINRR11 pKa = 11.84LTGRR15 pKa = 11.84RR16 pKa = 11.84WQYY19 pKa = 9.77QVEE22 pKa = 4.55FTYY25 pKa = 10.47TLQPGVPNSGTVSRR39 pKa = 11.84GMTLSVNDD47 pKa = 3.87RR48 pKa = 11.84NILADD53 pKa = 3.27HH54 pKa = 6.19RR55 pKa = 11.84HH56 pKa = 5.51IKK58 pKa = 9.84KK59 pKa = 10.48ALIPDD64 pKa = 4.41FVKK67 pKa = 10.43EE68 pKa = 4.3LPPHH72 pKa = 6.38LRR74 pKa = 11.84TNGALMISKK83 pKa = 9.92ISYY86 pKa = 9.52IGWFRR91 pKa = 11.84DD92 pKa = 3.0RR93 pKa = 11.84DD94 pKa = 3.63VRR96 pKa = 11.84PPQTGKK102 pKa = 10.44LGHH105 pKa = 6.72RR106 pKa = 11.84KK107 pKa = 9.06PFGALVV113 pKa = 3.26

Molecular weight: 12.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

161

0

161

33951

47

1310

210.9

23.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.92 ± 0.411

1.063 ± 0.089

6.339 ± 0.153

7.207 ± 0.238

3.349 ± 0.131

7.281 ± 0.23

2.106 ± 0.114

5.481 ± 0.152

6.333 ± 0.207

8.274 ± 0.156

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.998 ± 0.108

4.018 ± 0.154

4.026 ± 0.19

3.864 ± 0.161

5.367 ± 0.18

5.582 ± 0.13

5.85 ± 0.223

6.881 ± 0.197

1.376 ± 0.082

2.686 ± 0.097
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski