Amino acid dipepetide frequency for Pseudomonas phage nickie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.105AlaAla: 11.105 ± 1.13
1.09AlaCys: 1.09 ± 0.158
6.244AlaAsp: 6.244 ± 0.505
7.04AlaGlu: 7.04 ± 0.548
3.27AlaPhe: 3.27 ± 0.279
8.277AlaGly: 8.277 ± 0.6
2.062AlaHis: 2.062 ± 0.29
5.331AlaIle: 5.331 ± 0.431
6.745AlaLys: 6.745 ± 0.634
8.837AlaLeu: 8.837 ± 0.622
3.299AlaMet: 3.299 ± 0.33
3.918AlaAsn: 3.918 ± 0.323
3.093AlaPro: 3.093 ± 0.296
4.566AlaGln: 4.566 ± 0.451
4.566AlaArg: 4.566 ± 0.393
4.772AlaSer: 4.772 ± 0.386
5.037AlaThr: 5.037 ± 0.483
5.714AlaVal: 5.714 ± 0.445
1.355AlaTrp: 1.355 ± 0.169
2.887AlaTyr: 2.887 ± 0.277
0.0AlaXaa: 0.0 ± 0.0
Cys
0.736CysAla: 0.736 ± 0.147
0.059CysCys: 0.059 ± 0.036
0.854CysAsp: 0.854 ± 0.137
1.119CysGlu: 1.119 ± 0.16
0.236CysPhe: 0.236 ± 0.08
0.913CysGly: 0.913 ± 0.167
0.56CysHis: 0.56 ± 0.147
0.677CysIle: 0.677 ± 0.14
0.412CysLys: 0.412 ± 0.116
0.442CysLeu: 0.442 ± 0.114
0.088CysMet: 0.088 ± 0.051
0.383CysAsn: 0.383 ± 0.099
0.501CysPro: 0.501 ± 0.116
0.471CysGln: 0.471 ± 0.142
0.471CysArg: 0.471 ± 0.133
0.795CysSer: 0.795 ± 0.159
0.736CysThr: 0.736 ± 0.157
0.589CysVal: 0.589 ± 0.127
0.265CysTrp: 0.265 ± 0.082
0.324CysTyr: 0.324 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
5.979AspAla: 5.979 ± 0.477
0.854AspCys: 0.854 ± 0.209
4.507AspAsp: 4.507 ± 0.399
5.567AspGlu: 5.567 ± 0.449
2.003AspPhe: 2.003 ± 0.213
5.243AspGly: 5.243 ± 0.401
1.237AspHis: 1.237 ± 0.189
3.535AspIle: 3.535 ± 0.32
3.888AspLys: 3.888 ± 0.414
6.451AspLeu: 6.451 ± 0.481
1.532AspMet: 1.532 ± 0.247
1.915AspAsn: 1.915 ± 0.277
3.299AspPro: 3.299 ± 0.379
1.973AspGln: 1.973 ± 0.209
3.034AspArg: 3.034 ± 0.284
2.622AspSer: 2.622 ± 0.314
3.299AspThr: 3.299 ± 0.365
3.8AspVal: 3.8 ± 0.34
1.001AspTrp: 1.001 ± 0.158
1.649AspTyr: 1.649 ± 0.202
0.0AspXaa: 0.0 ± 0.0
Glu
7.953GluAla: 7.953 ± 0.534
0.884GluCys: 0.884 ± 0.145
4.3GluAsp: 4.3 ± 0.517
5.007GluGlu: 5.007 ± 0.479
2.504GluPhe: 2.504 ± 0.296
3.741GluGly: 3.741 ± 0.39
1.384GluHis: 1.384 ± 0.215
3.859GluIle: 3.859 ± 0.316
4.094GluLys: 4.094 ± 0.365
7.452GluLeu: 7.452 ± 0.454
2.15GluMet: 2.15 ± 0.283
2.651GluAsn: 2.651 ± 0.265
2.592GluPro: 2.592 ± 0.327
3.122GluGln: 3.122 ± 0.375
4.359GluArg: 4.359 ± 0.413
3.829GluSer: 3.829 ± 0.317
3.476GluThr: 3.476 ± 0.361
5.567GluVal: 5.567 ± 0.455
1.355GluTrp: 1.355 ± 0.218
1.767GluTyr: 1.767 ± 0.228
0.0GluXaa: 0.0 ± 0.0
Phe
2.828PheAla: 2.828 ± 0.337
0.442PheCys: 0.442 ± 0.107
3.122PheAsp: 3.122 ± 0.314
2.327PheGlu: 2.327 ± 0.255
0.884PhePhe: 0.884 ± 0.143
2.533PheGly: 2.533 ± 0.221
0.501PheHis: 0.501 ± 0.163
1.915PheIle: 1.915 ± 0.234
2.297PheLys: 2.297 ± 0.259
2.091PheLeu: 2.091 ± 0.273
0.825PheMet: 0.825 ± 0.146
1.679PheAsn: 1.679 ± 0.231
1.237PhePro: 1.237 ± 0.211
0.943PheGln: 0.943 ± 0.176
1.826PheArg: 1.826 ± 0.228
1.679PheSer: 1.679 ± 0.214
2.563PheThr: 2.563 ± 0.241
2.297PheVal: 2.297 ± 0.197
0.324PheTrp: 0.324 ± 0.107
1.178PheTyr: 1.178 ± 0.182
0.0PheXaa: 0.0 ± 0.0
Gly
6.009GlyAla: 6.009 ± 0.573
0.854GlyCys: 0.854 ± 0.159
4.33GlyAsp: 4.33 ± 0.397
4.536GlyGlu: 4.536 ± 0.419
3.299GlyPhe: 3.299 ± 0.367
6.539GlyGly: 6.539 ± 0.943
1.355GlyHis: 1.355 ± 0.199
4.654GlyIle: 4.654 ± 0.391
4.89GlyLys: 4.89 ± 0.378
5.979GlyLeu: 5.979 ± 0.314
2.239GlyMet: 2.239 ± 0.23
2.18GlyAsn: 2.18 ± 0.299
2.062GlyPro: 2.062 ± 0.272
2.474GlyGln: 2.474 ± 0.252
3.829GlyArg: 3.829 ± 0.286
4.477GlySer: 4.477 ± 0.305
4.89GlyThr: 4.89 ± 0.448
5.302GlyVal: 5.302 ± 0.356
1.267GlyTrp: 1.267 ± 0.181
1.649GlyTyr: 1.649 ± 0.23
0.0GlyXaa: 0.0 ± 0.0
His
1.915HisAla: 1.915 ± 0.224
0.265HisCys: 0.265 ± 0.078
1.237HisAsp: 1.237 ± 0.216
1.208HisGlu: 1.208 ± 0.164
0.795HisPhe: 0.795 ± 0.156
1.443HisGly: 1.443 ± 0.218
0.442HisHis: 0.442 ± 0.109
1.267HisIle: 1.267 ± 0.231
1.031HisLys: 1.031 ± 0.165
1.797HisLeu: 1.797 ± 0.24
0.353HisMet: 0.353 ± 0.086
1.09HisAsn: 1.09 ± 0.135
1.119HisPro: 1.119 ± 0.23
0.619HisGln: 0.619 ± 0.121
1.591HisArg: 1.591 ± 0.226
1.237HisSer: 1.237 ± 0.242
1.267HisThr: 1.267 ± 0.208
1.561HisVal: 1.561 ± 0.27
0.324HisTrp: 0.324 ± 0.106
0.501HisTyr: 0.501 ± 0.112
0.0HisXaa: 0.0 ± 0.0
Ile
5.567IleAla: 5.567 ± 0.404
0.589IleCys: 0.589 ± 0.126
4.242IleAsp: 4.242 ± 0.361
5.331IleGlu: 5.331 ± 0.357
1.208IlePhe: 1.208 ± 0.21
3.505IleGly: 3.505 ± 0.267
1.06IleHis: 1.06 ± 0.173
2.386IleIle: 2.386 ± 0.267
3.299IleLys: 3.299 ± 0.289
3.122IleLeu: 3.122 ± 0.289
1.679IleMet: 1.679 ± 0.201
2.415IleAsn: 2.415 ± 0.29
2.209IlePro: 2.209 ± 0.233
2.386IleGln: 2.386 ± 0.221
3.093IleArg: 3.093 ± 0.296
2.769IleSer: 2.769 ± 0.322
3.829IleThr: 3.829 ± 0.345
4.124IleVal: 4.124 ± 0.395
0.736IleTrp: 0.736 ± 0.15
1.267IleTyr: 1.267 ± 0.191
0.0IleXaa: 0.0 ± 0.0
Lys
7.541LysAla: 7.541 ± 0.603
0.353LysCys: 0.353 ± 0.125
3.299LysAsp: 3.299 ± 0.381
3.652LysGlu: 3.652 ± 0.301
1.708LysPhe: 1.708 ± 0.209
4.153LysGly: 4.153 ± 0.386
1.208LysHis: 1.208 ± 0.207
3.505LysIle: 3.505 ± 0.363
4.713LysLys: 4.713 ± 0.464
5.331LysLeu: 5.331 ± 0.425
2.209LysMet: 2.209 ± 0.276
2.798LysAsn: 2.798 ± 0.317
3.24LysPro: 3.24 ± 0.364
3.004LysGln: 3.004 ± 0.245
3.211LysArg: 3.211 ± 0.309
3.682LysSer: 3.682 ± 0.373
3.594LysThr: 3.594 ± 0.37
3.741LysVal: 3.741 ± 0.282
0.913LysTrp: 0.913 ± 0.149
1.473LysTyr: 1.473 ± 0.187
0.0LysXaa: 0.0 ± 0.0
Leu
7.747LeuAla: 7.747 ± 0.374
0.884LeuCys: 0.884 ± 0.156
5.744LeuAsp: 5.744 ± 0.443
6.775LeuGlu: 6.775 ± 0.424
2.739LeuPhe: 2.739 ± 0.31
4.624LeuGly: 4.624 ± 0.412
1.591LeuHis: 1.591 ± 0.217
4.477LeuIle: 4.477 ± 0.348
5.42LeuLys: 5.42 ± 0.368
6.186LeuLeu: 6.186 ± 0.457
2.474LeuMet: 2.474 ± 0.237
3.535LeuAsn: 3.535 ± 0.364
3.682LeuPro: 3.682 ± 0.367
3.505LeuGln: 3.505 ± 0.366
4.624LeuArg: 4.624 ± 0.279
5.125LeuSer: 5.125 ± 0.382
5.331LeuThr: 5.331 ± 0.401
5.361LeuVal: 5.361 ± 0.411
0.854LeuTrp: 0.854 ± 0.161
2.062LeuTyr: 2.062 ± 0.261
0.0LeuXaa: 0.0 ± 0.0
Met
3.063MetAla: 3.063 ± 0.319
0.471MetCys: 0.471 ± 0.116
1.502MetAsp: 1.502 ± 0.183
1.325MetGlu: 1.325 ± 0.209
1.031MetPhe: 1.031 ± 0.186
1.973MetGly: 1.973 ± 0.235
0.677MetHis: 0.677 ± 0.119
1.826MetIle: 1.826 ± 0.227
2.18MetLys: 2.18 ± 0.223
1.679MetLeu: 1.679 ± 0.23
0.972MetMet: 0.972 ± 0.148
1.826MetAsn: 1.826 ± 0.242
1.06MetPro: 1.06 ± 0.194
1.267MetGln: 1.267 ± 0.15
1.414MetArg: 1.414 ± 0.178
2.563MetSer: 2.563 ± 0.285
2.533MetThr: 2.533 ± 0.242
1.885MetVal: 1.885 ± 0.229
0.353MetTrp: 0.353 ± 0.1
0.383MetTyr: 0.383 ± 0.104
0.0MetXaa: 0.0 ± 0.0
Asn
3.976AsnAla: 3.976 ± 0.317
0.324AsnCys: 0.324 ± 0.095
2.327AsnAsp: 2.327 ± 0.261
1.826AsnGlu: 1.826 ± 0.217
1.767AsnPhe: 1.767 ± 0.203
3.476AsnGly: 3.476 ± 0.446
0.825AsnHis: 0.825 ± 0.155
1.767AsnIle: 1.767 ± 0.242
1.856AsnLys: 1.856 ± 0.218
3.829AsnLeu: 3.829 ± 0.378
1.443AsnMet: 1.443 ± 0.196
1.443AsnAsn: 1.443 ± 0.251
2.622AsnPro: 2.622 ± 0.266
1.797AsnGln: 1.797 ± 0.216
2.239AsnArg: 2.239 ± 0.244
2.209AsnSer: 2.209 ± 0.261
2.297AsnThr: 2.297 ± 0.317
2.386AsnVal: 2.386 ± 0.261
0.589AsnTrp: 0.589 ± 0.115
1.178AsnTyr: 1.178 ± 0.19
0.0AsnXaa: 0.0 ± 0.0
Pro
3.564ProAla: 3.564 ± 0.34
0.353ProCys: 0.353 ± 0.11
2.563ProAsp: 2.563 ± 0.252
4.389ProGlu: 4.389 ± 0.525
1.325ProPhe: 1.325 ± 0.194
3.358ProGly: 3.358 ± 0.276
0.648ProHis: 0.648 ± 0.101
1.973ProIle: 1.973 ± 0.229
2.739ProLys: 2.739 ± 0.299
3.063ProLeu: 3.063 ± 0.268
0.972ProMet: 0.972 ± 0.153
1.62ProAsn: 1.62 ± 0.195
1.325ProPro: 1.325 ± 0.193
1.208ProGln: 1.208 ± 0.201
1.62ProArg: 1.62 ± 0.229
2.15ProSer: 2.15 ± 0.255
2.415ProThr: 2.415 ± 0.244
3.505ProVal: 3.505 ± 0.371
0.295ProTrp: 0.295 ± 0.09
1.178ProTyr: 1.178 ± 0.167
0.0ProXaa: 0.0 ± 0.0
Gln
4.035GlnAla: 4.035 ± 0.323
0.412GlnCys: 0.412 ± 0.116
1.797GlnAsp: 1.797 ± 0.25
2.651GlnGlu: 2.651 ± 0.286
1.414GlnPhe: 1.414 ± 0.165
2.651GlnGly: 2.651 ± 0.3
0.766GlnHis: 0.766 ± 0.132
2.415GlnIle: 2.415 ± 0.291
2.563GlnLys: 2.563 ± 0.289
3.682GlnLeu: 3.682 ± 0.321
1.826GlnMet: 1.826 ± 0.258
1.178GlnAsn: 1.178 ± 0.19
1.296GlnPro: 1.296 ± 0.203
1.325GlnGln: 1.325 ± 0.22
2.386GlnArg: 2.386 ± 0.23
1.944GlnSer: 1.944 ± 0.226
2.121GlnThr: 2.121 ± 0.217
2.533GlnVal: 2.533 ± 0.234
0.501GlnTrp: 0.501 ± 0.107
1.149GlnTyr: 1.149 ± 0.192
0.0GlnXaa: 0.0 ± 0.0
Arg
5.155ArgAla: 5.155 ± 0.413
0.619ArgCys: 0.619 ± 0.11
2.916ArgAsp: 2.916 ± 0.314
4.065ArgGlu: 4.065 ± 0.427
2.062ArgPhe: 2.062 ± 0.211
3.004ArgGly: 3.004 ± 0.323
1.649ArgHis: 1.649 ± 0.242
2.887ArgIle: 2.887 ± 0.307
3.711ArgLys: 3.711 ± 0.295
4.595ArgLeu: 4.595 ± 0.393
1.532ArgMet: 1.532 ± 0.206
2.003ArgAsn: 2.003 ± 0.249
2.003ArgPro: 2.003 ± 0.269
2.297ArgGln: 2.297 ± 0.273
3.476ArgArg: 3.476 ± 0.435
2.386ArgSer: 2.386 ± 0.235
2.857ArgThr: 2.857 ± 0.265
4.183ArgVal: 4.183 ± 0.373
0.412ArgTrp: 0.412 ± 0.102
1.856ArgTyr: 1.856 ± 0.227
0.0ArgXaa: 0.0 ± 0.0
Ser
5.007SerAla: 5.007 ± 0.446
0.589SerCys: 0.589 ± 0.128
3.888SerAsp: 3.888 ± 0.349
3.476SerGlu: 3.476 ± 0.321
1.649SerPhe: 1.649 ± 0.194
4.713SerGly: 4.713 ± 0.36
1.355SerHis: 1.355 ± 0.238
2.798SerIle: 2.798 ± 0.257
3.888SerLys: 3.888 ± 0.33
4.919SerLeu: 4.919 ± 0.334
1.826SerMet: 1.826 ± 0.196
2.386SerAsn: 2.386 ± 0.263
2.032SerPro: 2.032 ± 0.229
2.297SerGln: 2.297 ± 0.256
2.946SerArg: 2.946 ± 0.271
3.505SerSer: 3.505 ± 0.67
2.386SerThr: 2.386 ± 0.243
3.947SerVal: 3.947 ± 0.329
0.619SerTrp: 0.619 ± 0.134
1.591SerTyr: 1.591 ± 0.184
0.0SerXaa: 0.0 ± 0.0
Thr
6.274ThrAla: 6.274 ± 0.765
0.442ThrCys: 0.442 ± 0.116
3.476ThrAsp: 3.476 ± 0.365
3.24ThrGlu: 3.24 ± 0.325
2.533ThrPhe: 2.533 ± 0.27
5.184ThrGly: 5.184 ± 0.407
1.267ThrHis: 1.267 ± 0.187
3.8ThrIle: 3.8 ± 0.382
3.417ThrLys: 3.417 ± 0.299
5.037ThrLeu: 5.037 ± 0.393
1.443ThrMet: 1.443 ± 0.19
2.445ThrAsn: 2.445 ± 0.285
2.415ThrPro: 2.415 ± 0.25
2.062ThrGln: 2.062 ± 0.246
2.68ThrArg: 2.68 ± 0.304
2.975ThrSer: 2.975 ± 0.317
2.857ThrThr: 2.857 ± 0.325
4.183ThrVal: 4.183 ± 0.428
1.208ThrTrp: 1.208 ± 0.171
1.561ThrTyr: 1.561 ± 0.201
0.0ThrXaa: 0.0 ± 0.0
Val
6.834ValAla: 6.834 ± 0.449
0.56ValCys: 0.56 ± 0.143
4.271ValAsp: 4.271 ± 0.367
5.685ValGlu: 5.685 ± 0.454
1.708ValPhe: 1.708 ± 0.207
4.242ValGly: 4.242 ± 0.348
1.502ValHis: 1.502 ± 0.182
3.594ValIle: 3.594 ± 0.323
4.183ValLys: 4.183 ± 0.374
4.654ValLeu: 4.654 ± 0.37
2.032ValMet: 2.032 ± 0.223
2.916ValAsn: 2.916 ± 0.303
3.093ValPro: 3.093 ± 0.311
1.885ValGln: 1.885 ± 0.239
3.682ValArg: 3.682 ± 0.378
4.418ValSer: 4.418 ± 0.435
4.566ValThr: 4.566 ± 0.396
5.773ValVal: 5.773 ± 0.416
0.972ValTrp: 0.972 ± 0.177
2.239ValTyr: 2.239 ± 0.274
0.0ValXaa: 0.0 ± 0.0
Trp
1.532TrpAla: 1.532 ± 0.189
0.118TrpCys: 0.118 ± 0.048
0.825TrpAsp: 0.825 ± 0.14
0.913TrpGlu: 0.913 ± 0.19
0.324TrpPhe: 0.324 ± 0.098
0.854TrpGly: 0.854 ± 0.147
0.501TrpHis: 0.501 ± 0.138
0.795TrpIle: 0.795 ± 0.121
0.766TrpLys: 0.766 ± 0.142
0.913TrpLeu: 0.913 ± 0.152
0.53TrpMet: 0.53 ± 0.12
0.53TrpAsn: 0.53 ± 0.116
0.324TrpPro: 0.324 ± 0.116
0.442TrpGln: 0.442 ± 0.126
0.884TrpArg: 0.884 ± 0.162
1.119TrpSer: 1.119 ± 0.14
0.795TrpThr: 0.795 ± 0.118
1.119TrpVal: 1.119 ± 0.196
0.118TrpTrp: 0.118 ± 0.059
0.353TrpTyr: 0.353 ± 0.091
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.386TyrAla: 2.386 ± 0.31
0.471TyrCys: 0.471 ± 0.115
1.944TyrAsp: 1.944 ± 0.212
1.944TyrGlu: 1.944 ± 0.226
1.031TyrPhe: 1.031 ± 0.19
2.386TyrGly: 2.386 ± 0.259
0.471TyrHis: 0.471 ± 0.136
1.355TyrIle: 1.355 ± 0.204
1.237TyrLys: 1.237 ± 0.174
2.68TyrLeu: 2.68 ± 0.291
0.53TyrMet: 0.53 ± 0.12
1.267TyrAsn: 1.267 ± 0.18
1.06TyrPro: 1.06 ± 0.212
1.001TyrGln: 1.001 ± 0.178
1.738TyrArg: 1.738 ± 0.324
1.561TyrSer: 1.561 ± 0.225
1.649TyrThr: 1.649 ± 0.173
1.237TyrVal: 1.237 ± 0.191
0.295TyrTrp: 0.295 ± 0.087
0.619TyrTyr: 0.619 ± 0.124
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 161 proteins (33951 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski