Polynucleobacter paneuropaeus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Polynucleobacter

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1853 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4JST9|A0A2Z4JST9_9BURK Glycine zipper family protein OS=Polynucleobacter paneuropaeus OX=2527775 GN=Pas1_05770 PE=4 SV=1
MM1 pKa = 7.41TYY3 pKa = 10.69VVTEE7 pKa = 3.66SCIRR11 pKa = 11.84CKK13 pKa = 10.34YY14 pKa = 7.74TDD16 pKa = 4.19CVDD19 pKa = 3.61VCPVDD24 pKa = 4.18CFRR27 pKa = 11.84EE28 pKa = 4.9GPNFLVIDD36 pKa = 4.26PDD38 pKa = 3.65EE39 pKa = 5.79CIDD42 pKa = 4.05CAVCVPEE49 pKa = 4.73CPVNAIYY56 pKa = 10.9AEE58 pKa = 4.42DD59 pKa = 4.72DD60 pKa = 3.81VPGDD64 pKa = 3.35QQQFIKK70 pKa = 10.66INADD74 pKa = 3.64LASSWTSITKK84 pKa = 10.4SKK86 pKa = 10.79GALPDD91 pKa = 3.62ADD93 pKa = 3.08EE94 pKa = 4.42WKK96 pKa = 10.5DD97 pKa = 3.72VKK99 pKa = 11.09SKK101 pKa = 10.62LAEE104 pKa = 3.92LVKK107 pKa = 11.02

Molecular weight: 11.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4JPX5|A0A2Z4JPX5_9BURK Phosphate import ATP-binding protein PstB OS=Polynucleobacter paneuropaeus OX=2527775 GN=pstB PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.17RR14 pKa = 11.84THH16 pKa = 5.95GFRR19 pKa = 11.84IRR21 pKa = 11.84MKK23 pKa = 8.63TKK25 pKa = 10.02SGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1853

0

1853

566203

38

5642

305.6

33.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.769 ± 0.059

0.956 ± 0.018

5.032 ± 0.044

5.662 ± 0.065

4.007 ± 0.045

7.544 ± 0.086

2.103 ± 0.033

6.518 ± 0.042

5.437 ± 0.06

10.465 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.575 ± 0.032

3.91 ± 0.062

4.558 ± 0.043

4.281 ± 0.038

4.845 ± 0.057

6.549 ± 0.093

5.149 ± 0.097

6.698 ± 0.046

1.282 ± 0.027

2.661 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski