Amino acid dipepetide frequency for Polynucleobacter paneuropaeus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.765AlaAla: 9.765 ± 0.196
1.141AlaCys: 1.141 ± 0.043
4.827AlaAsp: 4.827 ± 0.089
5.397AlaGlu: 5.397 ± 0.119
3.691AlaPhe: 3.691 ± 0.087
7.6AlaGly: 7.6 ± 0.156
1.846AlaHis: 1.846 ± 0.057
6.445AlaIle: 6.445 ± 0.136
5.913AlaLys: 5.913 ± 0.128
10.811AlaLeu: 10.811 ± 0.161
2.771AlaMet: 2.771 ± 0.082
3.889AlaAsn: 3.889 ± 0.116
3.91AlaPro: 3.91 ± 0.094
4.53AlaGln: 4.53 ± 0.114
4.516AlaArg: 4.516 ± 0.101
6.024AlaSer: 6.024 ± 0.133
4.532AlaThr: 4.532 ± 0.135
6.152AlaVal: 6.152 ± 0.121
1.28AlaTrp: 1.28 ± 0.05
2.651AlaTyr: 2.651 ± 0.069
0.0AlaXaa: 0.0 ± 0.0
Cys
0.959CysAla: 0.959 ± 0.039
0.12CysCys: 0.12 ± 0.014
0.548CysAsp: 0.548 ± 0.032
0.461CysGlu: 0.461 ± 0.032
0.403CysPhe: 0.403 ± 0.028
0.92CysGly: 0.92 ± 0.045
0.251CysHis: 0.251 ± 0.024
0.646CysIle: 0.646 ± 0.036
0.433CysLys: 0.433 ± 0.029
0.94CysLeu: 0.94 ± 0.039
0.193CysMet: 0.193 ± 0.019
0.32CysAsn: 0.32 ± 0.026
0.491CysPro: 0.491 ± 0.036
0.33CysGln: 0.33 ± 0.027
0.449CysArg: 0.449 ± 0.033
0.645CysSer: 0.645 ± 0.036
0.459CysThr: 0.459 ± 0.03
0.627CysVal: 0.627 ± 0.034
0.122CysTrp: 0.122 ± 0.013
0.247CysTyr: 0.247 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
4.845AspAla: 4.845 ± 0.104
0.526AspCys: 0.526 ± 0.031
2.289AspAsp: 2.289 ± 0.076
3.04AspGlu: 3.04 ± 0.089
2.202AspPhe: 2.202 ± 0.066
3.682AspGly: 3.682 ± 0.082
1.169AspHis: 1.169 ± 0.044
3.304AspIle: 3.304 ± 0.085
2.307AspLys: 2.307 ± 0.079
5.747AspLeu: 5.747 ± 0.112
1.116AspMet: 1.116 ± 0.038
1.383AspAsn: 1.383 ± 0.052
2.734AspPro: 2.734 ± 0.076
2.294AspGln: 2.294 ± 0.07
2.549AspArg: 2.549 ± 0.063
3.04AspSer: 3.04 ± 0.073
2.31AspThr: 2.31 ± 0.053
3.377AspVal: 3.377 ± 0.081
0.871AspTrp: 0.871 ± 0.04
1.537AspTyr: 1.537 ± 0.057
0.0AspXaa: 0.0 ± 0.0
Glu
5.634GluAla: 5.634 ± 0.11
0.457GluCys: 0.457 ± 0.032
2.552GluAsp: 2.552 ± 0.082
3.232GluGlu: 3.232 ± 0.102
2.236GluPhe: 2.236 ± 0.071
3.508GluGly: 3.508 ± 0.095
1.24GluHis: 1.24 ± 0.049
4.283GluIle: 4.283 ± 0.103
3.661GluLys: 3.661 ± 0.1
5.97GluLeu: 5.97 ± 0.126
1.528GluMet: 1.528 ± 0.058
2.063GluAsn: 2.063 ± 0.066
1.987GluPro: 1.987 ± 0.063
2.699GluGln: 2.699 ± 0.07
3.405GluArg: 3.405 ± 0.095
3.271GluSer: 3.271 ± 0.097
2.685GluThr: 2.685 ± 0.066
4.05GluVal: 4.05 ± 0.109
0.736GluTrp: 0.736 ± 0.039
1.42GluTyr: 1.42 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
3.928PheAla: 3.928 ± 0.087
0.528PheCys: 0.528 ± 0.031
2.448PheAsp: 2.448 ± 0.081
2.261PheGlu: 2.261 ± 0.068
1.816PhePhe: 1.816 ± 0.078
3.486PheGly: 3.486 ± 0.089
0.807PheHis: 0.807 ± 0.042
2.594PheIle: 2.594 ± 0.082
1.983PheLys: 1.983 ± 0.059
3.824PheLeu: 3.824 ± 0.11
0.885PheMet: 0.885 ± 0.043
1.634PheAsn: 1.634 ± 0.063
1.635PhePro: 1.635 ± 0.068
1.328PheGln: 1.328 ± 0.051
1.65PheArg: 1.65 ± 0.051
2.766PheSer: 2.766 ± 0.079
2.22PheThr: 2.22 ± 0.068
2.543PheVal: 2.543 ± 0.068
0.551PheTrp: 0.551 ± 0.034
1.18PheTyr: 1.18 ± 0.051
0.0PheXaa: 0.0 ± 0.0
Gly
6.473GlyAla: 6.473 ± 0.175
0.756GlyCys: 0.756 ± 0.037
3.672GlyAsp: 3.672 ± 0.084
3.901GlyGlu: 3.901 ± 0.1
3.416GlyPhe: 3.416 ± 0.083
6.058GlyGly: 6.058 ± 0.281
1.625GlyHis: 1.625 ± 0.047
5.208GlyIle: 5.208 ± 0.096
4.765GlyLys: 4.765 ± 0.103
7.971GlyLeu: 7.971 ± 0.142
2.218GlyMet: 2.218 ± 0.065
2.769GlyAsn: 2.769 ± 0.135
2.407GlyPro: 2.407 ± 0.076
2.997GlyGln: 2.997 ± 0.066
3.493GlyArg: 3.493 ± 0.083
5.102GlySer: 5.102 ± 0.228
3.762GlyThr: 3.762 ± 0.24
5.585GlyVal: 5.585 ± 0.103
1.012GlyTrp: 1.012 ± 0.048
2.248GlyTyr: 2.248 ± 0.074
0.0GlyXaa: 0.0 ± 0.0
His
2.04HisAla: 2.04 ± 0.062
0.263HisCys: 0.263 ± 0.021
1.047HisAsp: 1.047 ± 0.044
1.074HisGlu: 1.074 ± 0.044
0.996HisPhe: 0.996 ± 0.037
1.542HisGly: 1.542 ± 0.056
0.659HisHis: 0.659 ± 0.036
1.342HisIle: 1.342 ± 0.049
0.768HisLys: 0.768 ± 0.037
2.338HisLeu: 2.338 ± 0.066
0.487HisMet: 0.487 ± 0.032
0.609HisAsn: 0.609 ± 0.033
1.341HisPro: 1.341 ± 0.052
0.987HisGln: 0.987 ± 0.041
1.104HisArg: 1.104 ± 0.05
1.273HisSer: 1.273 ± 0.055
1.003HisThr: 1.003 ± 0.038
1.136HisVal: 1.136 ± 0.051
0.366HisTrp: 0.366 ± 0.027
0.659HisTyr: 0.659 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
7.222IleAla: 7.222 ± 0.118
0.691IleCys: 0.691 ± 0.033
3.937IleAsp: 3.937 ± 0.088
4.366IleGlu: 4.366 ± 0.096
2.462IlePhe: 2.462 ± 0.088
5.23IleGly: 5.23 ± 0.101
1.348IleHis: 1.348 ± 0.047
3.462IleIle: 3.462 ± 0.092
3.163IleLys: 3.163 ± 0.094
5.968IleLeu: 5.968 ± 0.119
1.242IleMet: 1.242 ± 0.047
2.607IleAsn: 2.607 ± 0.085
3.167IlePro: 3.167 ± 0.083
2.715IleGln: 2.715 ± 0.078
2.888IleArg: 2.888 ± 0.076
4.378IleSer: 4.378 ± 0.12
3.668IleThr: 3.668 ± 0.137
4.198IleVal: 4.198 ± 0.087
0.814IleTrp: 0.814 ± 0.041
1.658IleTyr: 1.658 ± 0.057
0.0IleXaa: 0.0 ± 0.0
Lys
5.417LysAla: 5.417 ± 0.108
0.306LysCys: 0.306 ± 0.023
2.771LysAsp: 2.771 ± 0.08
3.188LysGlu: 3.188 ± 0.093
1.496LysPhe: 1.496 ± 0.056
3.357LysGly: 3.357 ± 0.077
1.155LysHis: 1.155 ± 0.051
3.403LysIle: 3.403 ± 0.084
3.315LysLys: 3.315 ± 0.1
5.383LysLeu: 5.383 ± 0.104
1.727LysMet: 1.727 ± 0.057
2.31LysAsn: 2.31 ± 0.067
3.017LysPro: 3.017 ± 0.077
2.39LysGln: 2.39 ± 0.066
2.861LysArg: 2.861 ± 0.078
3.41LysSer: 3.41 ± 0.076
3.4LysThr: 3.4 ± 0.087
3.725LysVal: 3.725 ± 0.087
0.519LysTrp: 0.519 ± 0.033
1.215LysTyr: 1.215 ± 0.048
0.0LysXaa: 0.0 ± 0.0
Leu
10.978LeuAla: 10.978 ± 0.163
1.0LeuCys: 1.0 ± 0.042
5.457LeuAsp: 5.457 ± 0.113
6.046LeuGlu: 6.046 ± 0.144
3.856LeuPhe: 3.856 ± 0.105
7.909LeuGly: 7.909 ± 0.137
1.992LeuHis: 1.992 ± 0.055
6.733LeuIle: 6.733 ± 0.144
5.698LeuLys: 5.698 ± 0.121
10.625LeuLeu: 10.625 ± 0.224
2.658LeuMet: 2.658 ± 0.06
4.313LeuAsn: 4.313 ± 0.096
5.201LeuPro: 5.201 ± 0.116
4.203LeuGln: 4.203 ± 0.092
5.157LeuArg: 5.157 ± 0.126
7.405LeuSer: 7.405 ± 0.128
5.224LeuThr: 5.224 ± 0.131
6.72LeuVal: 6.72 ± 0.118
1.134LeuTrp: 1.134 ± 0.048
2.337LeuTyr: 2.337 ± 0.067
0.0LeuXaa: 0.0 ± 0.0
Met
2.602MetAla: 2.602 ± 0.084
0.159MetCys: 0.159 ± 0.017
1.107MetAsp: 1.107 ± 0.047
1.162MetGlu: 1.162 ± 0.049
0.774MetPhe: 0.774 ± 0.038
2.059MetGly: 2.059 ± 0.062
0.572MetHis: 0.572 ± 0.033
1.538MetIle: 1.538 ± 0.052
1.498MetLys: 1.498 ± 0.055
2.411MetLeu: 2.411 ± 0.06
0.724MetMet: 0.724 ± 0.039
1.349MetAsn: 1.349 ± 0.052
1.438MetPro: 1.438 ± 0.052
1.16MetGln: 1.16 ± 0.041
1.471MetArg: 1.471 ± 0.053
1.939MetSer: 1.939 ± 0.063
1.552MetThr: 1.552 ± 0.055
1.575MetVal: 1.575 ± 0.058
0.184MetTrp: 0.184 ± 0.017
0.477MetTyr: 0.477 ± 0.032
0.0MetXaa: 0.0 ± 0.0
Asn
3.765AsnAla: 3.765 ± 0.121
0.366AsnCys: 0.366 ± 0.023
1.602AsnAsp: 1.602 ± 0.054
1.837AsnGlu: 1.837 ± 0.063
1.51AsnPhe: 1.51 ± 0.061
2.934AsnGly: 2.934 ± 0.123
0.871AsnHis: 0.871 ± 0.043
2.388AsnIle: 2.388 ± 0.076
1.773AsnLys: 1.773 ± 0.059
4.147AsnLeu: 4.147 ± 0.092
0.88AsnMet: 0.88 ± 0.043
1.48AsnAsn: 1.48 ± 0.071
2.589AsnPro: 2.589 ± 0.07
1.89AsnGln: 1.89 ± 0.061
1.943AsnArg: 1.943 ± 0.066
2.549AsnSer: 2.549 ± 0.145
2.439AsnThr: 2.439 ± 0.105
2.372AsnVal: 2.372 ± 0.078
0.611AsnTrp: 0.611 ± 0.035
1.157AsnTyr: 1.157 ± 0.048
0.0AsnXaa: 0.0 ± 0.0
Pro
4.165ProAla: 4.165 ± 0.095
0.362ProCys: 0.362 ± 0.027
2.568ProAsp: 2.568 ± 0.084
3.326ProGlu: 3.326 ± 0.09
1.794ProPhe: 1.794 ± 0.054
3.303ProGly: 3.303 ± 0.081
0.954ProHis: 0.954 ± 0.045
3.004ProIle: 3.004 ± 0.076
2.63ProLys: 2.63 ± 0.073
4.491ProLeu: 4.491 ± 0.103
1.159ProMet: 1.159 ± 0.053
2.063ProAsn: 2.063 ± 0.053
1.63ProPro: 1.63 ± 0.069
1.913ProGln: 1.913 ± 0.06
1.745ProArg: 1.745 ± 0.063
2.944ProSer: 2.944 ± 0.065
2.347ProThr: 2.347 ± 0.059
3.345ProVal: 3.345 ± 0.097
0.535ProTrp: 0.535 ± 0.035
1.3ProTyr: 1.3 ± 0.052
0.002ProXaa: 0.002 ± 0.002
Gln
4.88GlnAla: 4.88 ± 0.117
0.366GlnCys: 0.366 ± 0.03
2.088GlnAsp: 2.088 ± 0.059
2.3GlnGlu: 2.3 ± 0.078
1.77GlnPhe: 1.77 ± 0.051
2.838GlnGly: 2.838 ± 0.071
0.834GlnHis: 0.834 ± 0.042
2.983GlnIle: 2.983 ± 0.07
2.473GlnLys: 2.473 ± 0.071
4.279GlnLeu: 4.279 ± 0.101
1.217GlnMet: 1.217 ± 0.044
1.756GlnAsn: 1.756 ± 0.059
1.409GlnPro: 1.409 ± 0.054
1.676GlnGln: 1.676 ± 0.065
2.065GlnArg: 2.065 ± 0.069
2.863GlnSer: 2.863 ± 0.084
2.314GlnThr: 2.314 ± 0.064
2.981GlnVal: 2.981 ± 0.079
0.565GlnTrp: 0.565 ± 0.033
1.152GlnTyr: 1.152 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
4.23ArgAla: 4.23 ± 0.098
0.381ArgCys: 0.381 ± 0.027
2.513ArgAsp: 2.513 ± 0.074
2.974ArgGlu: 2.974 ± 0.083
2.315ArgPhe: 2.315 ± 0.069
3.061ArgGly: 3.061 ± 0.083
1.111ArgHis: 1.111 ± 0.049
3.292ArgIle: 3.292 ± 0.079
2.563ArgLys: 2.563 ± 0.072
5.374ArgLeu: 5.374 ± 0.13
1.339ArgMet: 1.339 ± 0.054
1.959ArgAsn: 1.959 ± 0.056
2.075ArgPro: 2.075 ± 0.068
2.186ArgGln: 2.186 ± 0.065
2.533ArgArg: 2.533 ± 0.075
2.822ArgSer: 2.822 ± 0.084
2.098ArgThr: 2.098 ± 0.068
3.319ArgVal: 3.319 ± 0.088
0.653ArgTrp: 0.653 ± 0.028
1.646ArgTyr: 1.646 ± 0.057
0.0ArgXaa: 0.0 ± 0.0
Ser
6.335SerAla: 6.335 ± 0.167
0.569SerCys: 0.569 ± 0.032
3.123SerAsp: 3.123 ± 0.079
3.578SerGlu: 3.578 ± 0.086
2.616SerPhe: 2.616 ± 0.075
5.597SerGly: 5.597 ± 0.295
1.362SerHis: 1.362 ± 0.052
4.159SerIle: 4.159 ± 0.097
3.778SerLys: 3.778 ± 0.095
6.74SerLeu: 6.74 ± 0.132
1.66SerMet: 1.66 ± 0.044
2.649SerAsn: 2.649 ± 0.116
2.997SerPro: 2.997 ± 0.07
2.752SerGln: 2.752 ± 0.077
2.858SerArg: 2.858 ± 0.073
4.726SerSer: 4.726 ± 0.237
3.338SerThr: 3.338 ± 0.184
4.105SerVal: 4.105 ± 0.088
0.878SerTrp: 0.878 ± 0.042
1.674SerTyr: 1.674 ± 0.063
0.0SerXaa: 0.0 ± 0.0
Thr
4.85ThrAla: 4.85 ± 0.133
0.404ThrCys: 0.404 ± 0.031
2.273ThrAsp: 2.273 ± 0.069
2.575ThrGlu: 2.575 ± 0.074
2.036ThrPhe: 2.036 ± 0.064
4.421ThrGly: 4.421 ± 0.245
1.146ThrHis: 1.146 ± 0.046
3.359ThrIle: 3.359 ± 0.126
2.39ThrLys: 2.39 ± 0.073
5.781ThrLeu: 5.781 ± 0.167
1.155ThrMet: 1.155 ± 0.043
1.973ThrAsn: 1.973 ± 0.122
2.964ThrPro: 2.964 ± 0.08
2.278ThrGln: 2.278 ± 0.066
2.368ThrArg: 2.368 ± 0.062
3.267ThrSer: 3.267 ± 0.159
2.653ThrThr: 2.653 ± 0.18
3.569ThrVal: 3.569 ± 0.125
0.592ThrTrp: 0.592 ± 0.036
1.434ThrTyr: 1.434 ± 0.074
0.0ThrXaa: 0.0 ± 0.0
Val
6.268ValAla: 6.268 ± 0.126
0.673ValCys: 0.673 ± 0.034
3.64ValAsp: 3.64 ± 0.083
3.818ValGlu: 3.818 ± 0.091
2.69ValPhe: 2.69 ± 0.064
4.947ValGly: 4.947 ± 0.099
1.256ValHis: 1.256 ± 0.044
4.735ValIle: 4.735 ± 0.099
3.52ValLys: 3.52 ± 0.097
7.178ValLeu: 7.178 ± 0.127
1.923ValMet: 1.923 ± 0.058
2.676ValAsn: 2.676 ± 0.081
2.798ValPro: 2.798 ± 0.069
2.314ValGln: 2.314 ± 0.071
3.071ValArg: 3.071 ± 0.091
4.488ValSer: 4.488 ± 0.097
3.645ValThr: 3.645 ± 0.113
4.977ValVal: 4.977 ± 0.113
0.793ValTrp: 0.793 ± 0.037
1.568ValTyr: 1.568 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.98TrpAla: 0.98 ± 0.047
0.14TrpCys: 0.14 ± 0.015
0.604TrpAsp: 0.604 ± 0.031
0.586TrpGlu: 0.586 ± 0.032
0.631TrpPhe: 0.631 ± 0.034
0.819TrpGly: 0.819 ± 0.038
0.325TrpHis: 0.325 ± 0.024
0.906TrpIle: 0.906 ± 0.043
0.586TrpLys: 0.586 ± 0.031
1.648TrpLeu: 1.648 ± 0.059
0.369TrpMet: 0.369 ± 0.027
0.491TrpAsn: 0.491 ± 0.028
0.466TrpPro: 0.466 ± 0.032
0.724TrpGln: 0.724 ± 0.041
0.765TrpArg: 0.765 ± 0.039
0.745TrpSer: 0.745 ± 0.037
0.569TrpThr: 0.569 ± 0.036
0.941TrpVal: 0.941 ± 0.044
0.2TrpTrp: 0.2 ± 0.02
0.329TrpTyr: 0.329 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.356TyrAla: 2.356 ± 0.079
0.355TyrCys: 0.355 ± 0.027
1.256TyrAsp: 1.256 ± 0.044
1.494TyrGlu: 1.494 ± 0.055
1.358TyrPhe: 1.358 ± 0.051
2.169TyrGly: 2.169 ± 0.071
0.514TyrHis: 0.514 ± 0.033
1.395TyrIle: 1.395 ± 0.054
1.15TyrLys: 1.15 ± 0.052
3.022TyrLeu: 3.022 ± 0.075
0.5TyrMet: 0.5 ± 0.032
0.809TyrAsn: 0.809 ± 0.038
1.323TyrPro: 1.323 ± 0.054
1.439TyrGln: 1.439 ± 0.058
1.556TyrArg: 1.556 ± 0.061
1.832TyrSer: 1.832 ± 0.078
1.27TyrThr: 1.27 ± 0.069
1.681TyrVal: 1.681 ± 0.052
0.408TyrTrp: 0.408 ± 0.027
0.717TyrTyr: 0.717 ± 0.041
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.002XaaSer: 0.002 ± 0.002
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.002XaaXaa: 0.002 ± 0.002
Statistics based on 1853 proteins (566203 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski