Apis mellifera associated microvirus 44
Average proteome isoelectric point is 7.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3Q8U598|A0A3Q8U598_9VIRU Replication initiator protein OS=Apis mellifera associated microvirus 44 OX=2494774 PE=4 SV=1
MM1 pKa = 7.44 SSKK4 pKa = 10.51 KK5 pKa = 9.89 PSTEE9 pKa = 3.5 IPFDD13 pKa = 3.36 IAPRR17 pKa = 11.84 APRR20 pKa = 11.84 FQNLNTLGYY29 pKa = 9.95 SIADD33 pKa = 3.97 PTPVAPPIGYY43 pKa = 8.76 QKK45 pKa = 9.64 PISMVDD51 pKa = 2.88 QMRR54 pKa = 11.84 NLIRR58 pKa = 11.84 SEE60 pKa = 3.76 MLARR64 pKa = 11.84 AVAAQGMEE72 pKa = 4.1 TFEE75 pKa = 4.17 EE76 pKa = 4.67 SEE78 pKa = 4.58 DD79 pKa = 3.96 FDD81 pKa = 5.16 VPDD84 pKa = 5.47 DD85 pKa = 5.07 DD86 pKa = 5.95 GVLDD90 pKa = 4.1 PHH92 pKa = 7.09 SPWEE96 pKa = 4.03 NDD98 pKa = 2.61 FDD100 pKa = 4.31 PPARR104 pKa = 11.84 EE105 pKa = 3.54 IAEE108 pKa = 4.14 AVGDD112 pKa = 3.92 ARR114 pKa = 11.84 QRR116 pKa = 11.84 PPMPAEE122 pKa = 4.38 GGGGGAAPAEE132 pKa = 4.28 QPKK135 pKa = 9.45 PASPAPAPEE144 pKa = 4.58 APPAKK149 pKa = 9.7 PALPP153 pKa = 4.09
Molecular weight: 16.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.57
IPC2_protein 4.38
IPC_protein 4.304
Toseland 4.126
ProMoST 4.418
Dawson 4.253
Bjellqvist 4.406
Wikipedia 4.151
Rodwell 4.139
Grimsley 4.037
Solomon 4.253
Lehninger 4.202
Nozaki 4.368
DTASelect 4.546
Thurlkill 4.139
EMBOSS 4.164
Sillero 4.418
Patrickios 4.164
IPC_peptide 4.253
IPC2_peptide 4.393
IPC2.peptide.svr19 4.359
Protein with the highest isoelectric point:
>tr|A0A3S8UTI2|A0A3S8UTI2_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 44 OX=2494774 PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 10.6 GGGLNGLSSSSSSDD16 pKa = 3.16 CSSSSRR22 pKa = 11.84 SRR24 pKa = 11.84 SSSSRR29 pKa = 11.84 SRR31 pKa = 11.84 APNRR35 pKa = 11.84 VSHH38 pKa = 5.79 VRR40 pKa = 11.84 CEE42 pKa = 4.1 NPFVRR47 pKa = 11.84 GNSVHH52 pKa = 6.25 GCGRR56 pKa = 11.84 CLPCRR61 pKa = 11.84 INRR64 pKa = 11.84 RR65 pKa = 11.84 RR66 pKa = 11.84 LWKK69 pKa = 10.4 VRR71 pKa = 11.84 LGLEE75 pKa = 4.24 ALLHH79 pKa = 5.36 SQSAFVTLTYY89 pKa = 10.72 ADD91 pKa = 3.89 EE92 pKa = 4.42 NLPVAKK98 pKa = 10.11 SSKK101 pKa = 10.61 GSLATLDD108 pKa = 4.0 PVDD111 pKa = 3.94 LRR113 pKa = 11.84 NWLKK117 pKa = 10.64 RR118 pKa = 11.84 LRR120 pKa = 11.84 KK121 pKa = 9.6 AISPSSIRR129 pKa = 11.84 YY130 pKa = 7.64 YY131 pKa = 11.24 AVGEE135 pKa = 4.2 YY136 pKa = 10.63 GDD138 pKa = 4.16 EE139 pKa = 4.0 SWRR142 pKa = 11.84 PHH144 pKa = 4.14 YY145 pKa = 10.57 HH146 pKa = 6.46 LALFGYY152 pKa = 7.51 PRR154 pKa = 11.84 CSTLQTTYY162 pKa = 11.01 RR163 pKa = 11.84 LRR165 pKa = 11.84 ASTSEE170 pKa = 3.97 KK171 pKa = 10.34 PKK173 pKa = 10.95 CCDD176 pKa = 2.82 HH177 pKa = 6.88 CRR179 pKa = 11.84 LIHH182 pKa = 6.93 DD183 pKa = 3.53 SWGLGRR189 pKa = 11.84 VQVAEE194 pKa = 4.75 LNDD197 pKa = 3.43 ASAGYY202 pKa = 9.38 IAGYY206 pKa = 9.4 IEE208 pKa = 4.63 KK209 pKa = 10.41 KK210 pKa = 9.06 LTRR213 pKa = 11.84 TDD215 pKa = 3.55 DD216 pKa = 3.37 PRR218 pKa = 11.84 LDD220 pKa = 3.79 GRR222 pKa = 11.84 WPEE225 pKa = 4.01 FARR228 pKa = 11.84 MSLRR232 pKa = 11.84 PGIGAGFMPHH242 pKa = 6.44 YY243 pKa = 9.92 FQALLEE249 pKa = 4.58 HH250 pKa = 6.64 NLDD253 pKa = 3.63 GMADD257 pKa = 3.55 VPSAIRR263 pKa = 11.84 IGDD266 pKa = 3.57 KK267 pKa = 10.41 VVPLGRR273 pKa = 11.84 YY274 pKa = 7.46 LTSQLRR280 pKa = 11.84 KK281 pKa = 10.31 LNGKK285 pKa = 9.12 DD286 pKa = 3.12 GKK288 pKa = 10.78 APPEE292 pKa = 3.7 TLKK295 pKa = 11.09 AVFEE299 pKa = 4.44 EE300 pKa = 4.69 KK301 pKa = 10.69 VLPLLLAARR310 pKa = 11.84 TSADD314 pKa = 3.31 APSLKK319 pKa = 10.49 EE320 pKa = 4.05 QVLQKK325 pKa = 10.86 NRR327 pKa = 11.84 GARR330 pKa = 11.84 SRR332 pKa = 11.84 LLSLNKK338 pKa = 9.25 IHH340 pKa = 7.12 RR341 pKa = 11.84 QRR343 pKa = 11.84 KK344 pKa = 7.8 HH345 pKa = 4.65 LL346 pKa = 4.02
Molecular weight: 38.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.293
IPC2_protein 9.428
IPC_protein 9.692
Toseland 10.233
ProMoST 9.911
Dawson 10.394
Bjellqvist 10.101
Wikipedia 10.57
Rodwell 10.716
Grimsley 10.467
Solomon 10.438
Lehninger 10.409
Nozaki 10.277
DTASelect 10.072
Thurlkill 10.262
EMBOSS 10.628
Sillero 10.321
Patrickios 10.335
IPC_peptide 10.438
IPC2_peptide 9.107
IPC2.peptide.svr19 8.423
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1420
75
499
284.0
31.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.0 ± 1.423
0.845 ± 0.551
5.07 ± 0.575
4.507 ± 0.509
3.732 ± 0.68
8.099 ± 0.717
2.394 ± 0.334
4.085 ± 0.742
4.085 ± 0.737
7.746 ± 1.479
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.746 ± 0.613
4.366 ± 0.569
7.324 ± 1.679
4.296 ± 0.872
7.746 ± 1.367
8.028 ± 1.071
4.93 ± 1.017
5.211 ± 0.462
1.338 ± 0.278
3.451 ± 0.513
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here