Maize mosaic virus (isolate Maize/United States/Reed/2005) (MMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Nucleorhabdovirus; Maize mosaic nucleorhabdovirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q6E0X1|NCAP_MMVR Nucleoprotein OS=Maize mosaic virus (isolate Maize/United States/Reed/2005) OX=928305 GN=N PE=3 SV=1
MM1 pKa = 7.09NRR3 pKa = 11.84YY4 pKa = 8.65SRR6 pKa = 11.84RR7 pKa = 11.84SRR9 pKa = 11.84HH10 pKa = 5.9PNPPVPNQEE19 pKa = 4.02EE20 pKa = 4.56PEE22 pKa = 4.1RR23 pKa = 11.84DD24 pKa = 3.77PNHH27 pKa = 6.96IDD29 pKa = 3.82QDD31 pKa = 4.33LADD34 pKa = 4.55LAQPLVLKK42 pKa = 10.04EE43 pKa = 3.94RR44 pKa = 11.84HH45 pKa = 5.76AVMAPTQPSLSDD57 pKa = 3.5VINEE61 pKa = 3.77EE62 pKa = 3.77RR63 pKa = 11.84QAPITFGNPPEE74 pKa = 4.23VMANARR80 pKa = 11.84LSALGYY86 pKa = 10.87DD87 pKa = 3.43NLTEE91 pKa = 3.99RR92 pKa = 11.84EE93 pKa = 3.96KK94 pKa = 10.94RR95 pKa = 11.84ILAVGVRR102 pKa = 11.84CGEE105 pKa = 3.87AAKK108 pKa = 10.19DD109 pKa = 3.77YY110 pKa = 11.32HH111 pKa = 7.73SLTTTKK117 pKa = 10.38KK118 pKa = 9.62WIEE121 pKa = 4.51DD122 pKa = 3.56EE123 pKa = 5.29LKK125 pKa = 10.6SQMVALASSTRR136 pKa = 11.84TLTEE140 pKa = 3.65AASLHH145 pKa = 4.98TTFAMLHH152 pKa = 5.7SPSIKK157 pKa = 9.96RR158 pKa = 11.84KK159 pKa = 9.49AEE161 pKa = 3.97AMSHH165 pKa = 5.99ISQGEE170 pKa = 3.82EE171 pKa = 3.77SIDD174 pKa = 3.2ISKK177 pKa = 10.72LNKK180 pKa = 9.61TGMEE184 pKa = 4.85DD185 pKa = 2.28IWVAMEE191 pKa = 4.33SEE193 pKa = 4.4SKK195 pKa = 10.65EE196 pKa = 4.24DD197 pKa = 4.61AVDD200 pKa = 3.52TYY202 pKa = 11.31LRR204 pKa = 11.84NILEE208 pKa = 4.03VDD210 pKa = 3.32PTQFYY215 pKa = 10.83AIDD218 pKa = 3.38GWGLYY223 pKa = 10.52LDD225 pKa = 6.52FIPTWHH231 pKa = 6.76YY232 pKa = 10.4IAAGKK237 pKa = 10.29NSAQFKK243 pKa = 9.53TSYY246 pKa = 10.66ADD248 pKa = 3.66EE249 pKa = 4.34IVEE252 pKa = 3.77QRR254 pKa = 11.84AAFEE258 pKa = 4.31RR259 pKa = 11.84VLSKK263 pKa = 10.64RR264 pKa = 11.84PRR266 pKa = 11.84VEE268 pKa = 3.33II269 pKa = 4.27

Molecular weight: 30.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q6E0X1|NCAP_MMVR Nucleoprotein OS=Maize mosaic virus (isolate Maize/United States/Reed/2005) OX=928305 GN=N PE=3 SV=1
MM1 pKa = 7.59ANINIPDD8 pKa = 4.29DD9 pKa = 4.02LVSKK13 pKa = 10.69YY14 pKa = 10.91SEE16 pKa = 4.23DD17 pKa = 4.61VKK19 pKa = 11.44DD20 pKa = 3.82LTQKK24 pKa = 10.77AGSIPSSKK32 pKa = 10.92SLIPQTAFTPDD43 pKa = 3.01EE44 pKa = 4.28LRR46 pKa = 11.84RR47 pKa = 11.84SLKK50 pKa = 9.76FWQVTTKK57 pKa = 10.52VAATVASDD65 pKa = 3.2WTNLATALTNGTFSATHH82 pKa = 6.31LRR84 pKa = 11.84TLCEE88 pKa = 3.55LAFNLKK94 pKa = 10.47APTGTGTIFIHH105 pKa = 7.06EE106 pKa = 4.68IADD109 pKa = 3.54PWKK112 pKa = 10.86GCIDD116 pKa = 3.72ANAPDD121 pKa = 4.31TVAIPATDD129 pKa = 3.26SDD131 pKa = 4.6VNMSTVSSGSTASGTEE147 pKa = 3.83DD148 pKa = 4.7AEE150 pKa = 4.62VKK152 pKa = 10.0MKK154 pKa = 10.71AISFLCCTLLRR165 pKa = 11.84LSVKK169 pKa = 9.82EE170 pKa = 4.39PSHH173 pKa = 7.67IMQAINSIRR182 pKa = 11.84QRR184 pKa = 11.84FGSLYY189 pKa = 10.06GVSSPTLNSVTFNRR203 pKa = 11.84SQLSRR208 pKa = 11.84IKK210 pKa = 10.5QGIEE214 pKa = 3.9TYY216 pKa = 9.8PSARR220 pKa = 11.84GTVFYY225 pKa = 8.14YY226 pKa = 10.24TRR228 pKa = 11.84YY229 pKa = 10.36ADD231 pKa = 3.44ATHH234 pKa = 7.11GYY236 pKa = 5.28TTKK239 pKa = 10.58EE240 pKa = 3.56YY241 pKa = 10.42GICRR245 pKa = 11.84FLLFQHH251 pKa = 6.78LEE253 pKa = 4.08LEE255 pKa = 4.52GMHH258 pKa = 6.77IYY260 pKa = 11.04KK261 pKa = 9.5MLLALLGEE269 pKa = 4.66WSTVPIGLLLTWIRR283 pKa = 11.84NPRR286 pKa = 11.84SKK288 pKa = 10.72LAINEE293 pKa = 3.63IVKK296 pKa = 9.9IVKK299 pKa = 9.79EE300 pKa = 3.85LDD302 pKa = 3.21KK303 pKa = 11.59PEE305 pKa = 3.89VDD307 pKa = 4.73KK308 pKa = 11.1GWKK311 pKa = 7.57YY312 pKa = 11.42ARR314 pKa = 11.84MVNNTFFLDD323 pKa = 3.98LSSRR327 pKa = 11.84RR328 pKa = 11.84NTYY331 pKa = 10.14LCAVLASLNKK341 pKa = 10.06KK342 pKa = 9.32NVPQGSGEE350 pKa = 4.15YY351 pKa = 10.49ADD353 pKa = 3.88PTNIAVIKK361 pKa = 11.17NMDD364 pKa = 3.56QSVKK368 pKa = 10.55SQVATDD374 pKa = 3.26VTLIEE379 pKa = 4.83RR380 pKa = 11.84IYY382 pKa = 10.98EE383 pKa = 4.16KK384 pKa = 11.1YY385 pKa = 10.35LVSAGGDD392 pKa = 3.56EE393 pKa = 5.15AGTAYY398 pKa = 10.55ALSRR402 pKa = 11.84GVKK405 pKa = 9.66RR406 pKa = 11.84SSPPSQEE413 pKa = 4.14GGQSGQSGGTPMEE426 pKa = 3.86VDD428 pKa = 3.55GASGRR433 pKa = 11.84GAAGPAPKK441 pKa = 8.91KK442 pKa = 8.96TRR444 pKa = 11.84SGLL447 pKa = 3.57

Molecular weight: 48.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3750

235

1922

625.0

69.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.187 ± 0.685

1.627 ± 0.236

5.6 ± 0.344

5.68 ± 0.478

3.04 ± 0.204

6.427 ± 0.458

2.667 ± 0.223

7.093 ± 0.45

5.253 ± 0.186

10.16 ± 0.741

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.8 ± 0.242

3.867 ± 0.265

4.453 ± 0.426

2.747 ± 0.234

5.44 ± 0.598

8.773 ± 0.798

7.013 ± 0.558

5.893 ± 0.239

1.787 ± 0.331

3.413 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski