Lake Sarah-associated circular virus-40

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQQ2|A0A140AQQ2_9VIRU Coat protein OS=Lake Sarah-associated circular virus-40 OX=1685769 PE=4 SV=1
MM1 pKa = 7.85EE2 pKa = 4.36EE3 pKa = 3.92TTTEE7 pKa = 3.86KK8 pKa = 10.88SVISTGGGPQSSRR21 pKa = 11.84TWCGTLNNYY30 pKa = 8.68SQGEE34 pKa = 4.32YY35 pKa = 10.55EE36 pKa = 4.66FLVQEE41 pKa = 4.25FNKK44 pKa = 9.72VAEE47 pKa = 3.86YY48 pKa = 9.79WIIGKK53 pKa = 9.63EE54 pKa = 3.9KK55 pKa = 9.37GQEE58 pKa = 4.22GTPHH62 pKa = 6.59LQMACSFTRR71 pKa = 11.84AQKK74 pKa = 10.82LNYY77 pKa = 9.47LKK79 pKa = 10.85QNLSVRR85 pKa = 11.84AHH87 pKa = 5.65WEE89 pKa = 3.79ITKK92 pKa = 8.89GTLDD96 pKa = 3.48QNRR99 pKa = 11.84VYY101 pKa = 10.85CMKK104 pKa = 10.63EE105 pKa = 3.28GDD107 pKa = 4.03YY108 pKa = 10.97TEE110 pKa = 5.95GGAPPVKK117 pKa = 10.24KK118 pKa = 9.99QGKK121 pKa = 7.51RR122 pKa = 11.84TDD124 pKa = 2.82IEE126 pKa = 4.34MAVEE130 pKa = 4.02TLKK133 pKa = 10.63ATGGDD138 pKa = 3.79VQEE141 pKa = 4.65MALTHH146 pKa = 6.28PEE148 pKa = 4.0AYY150 pKa = 9.77VKK152 pKa = 10.86YY153 pKa = 10.22SSNFEE158 pKa = 4.13KK159 pKa = 10.71LAQRR163 pKa = 11.84YY164 pKa = 4.84QKK166 pKa = 10.87KK167 pKa = 8.66MRR169 pKa = 11.84SSGSGYY175 pKa = 8.59VHH177 pKa = 7.4DD178 pKa = 5.52RR179 pKa = 11.84RR180 pKa = 11.84VIWIYY185 pKa = 10.95GPSGTGKK192 pKa = 7.95TRR194 pKa = 11.84YY195 pKa = 8.23VHH197 pKa = 6.25EE198 pKa = 5.27KK199 pKa = 10.65EE200 pKa = 5.01LISNLWISGRR210 pKa = 11.84DD211 pKa = 3.6LRR213 pKa = 11.84WWQSYY218 pKa = 10.77DD219 pKa = 3.36MEE221 pKa = 4.74PAVLFDD227 pKa = 4.23DD228 pKa = 5.23FRR230 pKa = 11.84GDD232 pKa = 3.03FCKK235 pKa = 10.58YY236 pKa = 10.31HH237 pKa = 6.18EE238 pKa = 5.03LLRR241 pKa = 11.84ILDD244 pKa = 4.48RR245 pKa = 11.84YY246 pKa = 9.59QCTVEE251 pKa = 4.07NKK253 pKa = 9.86GGSVSLQNVKK263 pKa = 10.47RR264 pKa = 11.84IWITSCFHH272 pKa = 6.47PRR274 pKa = 11.84HH275 pKa = 5.97VYY277 pKa = 8.23DD278 pKa = 3.2TRR280 pKa = 11.84EE281 pKa = 4.65DD282 pKa = 3.42IEE284 pKa = 4.08QLIRR288 pKa = 11.84RR289 pKa = 11.84IDD291 pKa = 4.1KK292 pKa = 9.5IWHH295 pKa = 5.1FTSTTKK301 pKa = 10.61TDD303 pKa = 3.24VTNEE307 pKa = 3.53KK308 pKa = 9.81EE309 pKa = 4.44YY310 pKa = 10.34PGKK313 pKa = 9.87PGGAAPDD320 pKa = 5.2FITPLALVRR329 pKa = 11.84TKK331 pKa = 10.98AVIPEE336 pKa = 4.25SPPTPTNSRR345 pKa = 11.84PNSKK349 pKa = 10.05RR350 pKa = 11.84SSTIGTEE357 pKa = 3.51LDD359 pKa = 3.45MVISRR364 pKa = 11.84GVATQPLVVLDD375 pKa = 3.82SDD377 pKa = 4.33EE378 pKa = 4.85EE379 pKa = 4.43EE380 pKa = 4.47EE381 pKa = 5.99LPGQPDD387 pKa = 3.79SSWAASQWKK396 pKa = 9.57RR397 pKa = 11.84AVKK400 pKa = 10.29KK401 pKa = 10.94SEE403 pKa = 3.63

Molecular weight: 45.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQQ2|A0A140AQQ2_9VIRU Coat protein OS=Lake Sarah-associated circular virus-40 OX=1685769 PE=4 SV=1
MM1 pKa = 7.23PGKK4 pKa = 9.84RR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 9.82YY8 pKa = 10.17SLGSKK13 pKa = 9.32AQIRR17 pKa = 11.84KK18 pKa = 6.8MARR21 pKa = 11.84AYY23 pKa = 9.99VRR25 pKa = 11.84KK26 pKa = 9.69KK27 pKa = 10.04YY28 pKa = 9.37RR29 pKa = 11.84TGGVPVTRR37 pKa = 11.84GAYY40 pKa = 8.96NMRR43 pKa = 11.84MPSEE47 pKa = 4.57LKK49 pKa = 10.59LSQNQTLGLTTLQNTPTIANNQLILLNSIAQGTDD83 pKa = 2.8YY84 pKa = 7.56TTRR87 pKa = 11.84IGRR90 pKa = 11.84TCLATSLQWNLEE102 pKa = 4.05LFQAPGTTTSNNQIWVRR119 pKa = 11.84AMVFCDD125 pKa = 3.37RR126 pKa = 11.84QPNGVEE132 pKa = 3.55PSVFSDD138 pKa = 3.51VLDD141 pKa = 3.49STFRR145 pKa = 11.84TEE147 pKa = 3.62QAFRR151 pKa = 11.84NLSNRR156 pKa = 11.84DD157 pKa = 2.94RR158 pKa = 11.84FVIVANKK165 pKa = 8.07VWSWSDD171 pKa = 2.81WNTLQPEE178 pKa = 4.49SLGCAKK184 pKa = 10.74NGGPKK189 pKa = 9.49RR190 pKa = 11.84WAKK193 pKa = 9.75YY194 pKa = 10.02KK195 pKa = 10.38KK196 pKa = 10.4LNVEE200 pKa = 3.84KK201 pKa = 9.43TVYY204 pKa = 10.29SGTSGGITNVQSCALFFAIWLVNTSGAVQANFAYY238 pKa = 10.43RR239 pKa = 11.84LRR241 pKa = 11.84FVDD244 pKa = 5.09FF245 pKa = 4.21

Molecular weight: 27.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

648

245

403

324.0

36.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.71 ± 0.934

1.543 ± 0.051

4.167 ± 0.747

6.019 ± 2.036

3.241 ± 0.712

7.253 ± 0.179

1.389 ± 0.792

4.475 ± 0.457

6.79 ± 0.614

7.099 ± 0.84

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.006 ± 0.02

4.938 ± 1.607

4.475 ± 0.457

5.093 ± 0.355

6.79 ± 0.55

7.562 ± 0.123

8.333 ± 0.369

6.481 ± 0.261

2.778 ± 0.045

3.858 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski