Sulfitobacter phage phiCB2047-B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Rhodovirinae; Raunefjordenvirus; Sulfitobacter virus phiCB2047B

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4PMS3|M4PMS3_9CAUD Uncharacterized protein OS=Sulfitobacter phage phiCB2047-B OX=754046 GN=SUFG_00034 PE=4 SV=1
MM1 pKa = 8.35SEE3 pKa = 3.77MEE5 pKa = 4.38MFIGTAKK12 pKa = 10.36RR13 pKa = 11.84YY14 pKa = 8.69VGPDD18 pKa = 2.79IKK20 pKa = 11.32VEE22 pKa = 4.1DD23 pKa = 3.62TDD25 pKa = 3.43EE26 pKa = 4.83FYY28 pKa = 11.1EE29 pKa = 4.36LEE31 pKa = 4.07QEE33 pKa = 4.46LGHH36 pKa = 5.86SAIVKK41 pKa = 10.37VGDD44 pKa = 3.99EE45 pKa = 4.18LWCIDD50 pKa = 3.77KK51 pKa = 11.46LEE53 pKa = 4.75DD54 pKa = 2.97VDD56 pKa = 5.56AYY58 pKa = 10.77GFQTIIEE65 pKa = 4.34PQSEE69 pKa = 4.39LMFIGYY75 pKa = 8.44WYY77 pKa = 10.57NGGGGIHH84 pKa = 6.22EE85 pKa = 4.61VSASAIKK92 pKa = 10.43DD93 pKa = 3.52YY94 pKa = 11.21LKK96 pKa = 11.02SKK98 pKa = 10.6EE99 pKa = 4.02EE100 pKa = 4.07EE101 pKa = 4.05EE102 pKa = 4.33

Molecular weight: 11.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4PRQ3|M4PRQ3_9CAUD Uncharacterized protein OS=Sulfitobacter phage phiCB2047-B OX=754046 GN=SUFG_00064 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84DD3 pKa = 4.39DD4 pKa = 4.53IPTSTVSDD12 pKa = 3.83RR13 pKa = 11.84NHH15 pKa = 8.01LEE17 pKa = 3.77MEE19 pKa = 4.19YY20 pKa = 10.95EE21 pKa = 4.5DD22 pKa = 3.89YY23 pKa = 11.12HH24 pKa = 8.59DD25 pKa = 4.24LAEE28 pKa = 4.6AVSKK32 pKa = 11.23NCIRR36 pKa = 11.84DD37 pKa = 3.21RR38 pKa = 11.84FKK40 pKa = 11.15NHH42 pKa = 4.44MPIRR46 pKa = 11.84KK47 pKa = 8.78LKK49 pKa = 9.39IRR51 pKa = 11.84KK52 pKa = 7.02IHH54 pKa = 5.34NMRR57 pKa = 11.84NRR59 pKa = 11.84IIKK62 pKa = 9.38SEE64 pKa = 4.11KK65 pKa = 9.74LSKK68 pKa = 10.81SLIFKK73 pKa = 10.54EE74 pKa = 4.23MLRR77 pKa = 11.84QLNQEE82 pKa = 3.58LSKK85 pKa = 10.26RR86 pKa = 11.84KK87 pKa = 9.67KK88 pKa = 8.71MKK90 pKa = 10.51GMDD93 pKa = 3.17RR94 pKa = 11.84RR95 pKa = 11.84IPSS98 pKa = 3.14

Molecular weight: 11.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

22169

52

3330

295.6

33.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.077 ± 0.352

0.65 ± 0.13

6.789 ± 0.169

7.141 ± 0.233

3.848 ± 0.159

6.59 ± 0.225

1.646 ± 0.178

5.869 ± 0.216

6.617 ± 0.332

7.858 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.126 ± 0.137

5.688 ± 0.23

3.933 ± 0.18

4.028 ± 0.246

4.348 ± 0.195

6.902 ± 0.222

6.518 ± 0.222

6.78 ± 0.238

1.074 ± 0.124

3.518 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski