Aliifodinibius sediminis
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3822 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A521B7J8|A0A521B7J8_9BACT Uncharacterized protein OS=Aliifodinibius sediminis OX=1214077 GN=SAMN06265218_102263 PE=4 SV=1
MM1 pKa = 7.32 QSIVNNRR8 pKa = 11.84 FCLLPVILLISSLALTSCLDD28 pKa = 3.2 SSTDD32 pKa = 3.53 TNQGASKK39 pKa = 10.79 NLLEE43 pKa = 4.26 QAQSYY48 pKa = 11.42 ADD50 pKa = 3.71 FDD52 pKa = 4.27 TFVDD56 pKa = 4.73 IISDD60 pKa = 3.91 TEE62 pKa = 4.42 LNTTIANEE70 pKa = 4.51 GPLTLLVPTNDD81 pKa = 4.11 AFDD84 pKa = 4.6 ALPSGTLEE92 pKa = 4.34 SLTDD96 pKa = 3.52 EE97 pKa = 4.08 QLIDD101 pKa = 3.21 ILSYY105 pKa = 10.79 HH106 pKa = 6.82 VIEE109 pKa = 5.59 QIIDD113 pKa = 3.78 LNQLSAEE120 pKa = 4.18 EE121 pKa = 5.03 TFTSMQGDD129 pKa = 3.6 NIFFVISNDD138 pKa = 3.01 SLYY141 pKa = 10.49 INGSNYY147 pKa = 10.08 VGGVGASNGVLYY159 pKa = 9.01 ATDD162 pKa = 3.27 QVLFPDD168 pKa = 4.78 AYY170 pKa = 11.24 LDD172 pKa = 3.75 VTGLVHH178 pKa = 7.42 KK179 pKa = 10.44 RR180 pKa = 11.84 KK181 pKa = 9.35 QLSEE185 pKa = 4.05 LDD187 pKa = 3.55 KK188 pKa = 11.54 SIDD191 pKa = 3.6 NTGLASTLEE200 pKa = 4.48 DD201 pKa = 3.05 TSSAYY206 pKa = 9.75 TIFAPSNTALQNAEE220 pKa = 4.39 LSADD224 pKa = 3.5 TLKK227 pKa = 10.98 YY228 pKa = 10.24 HH229 pKa = 5.65 VVSGKK234 pKa = 10.21 LLSQDD239 pKa = 3.62 FSSSQTYY246 pKa = 6.76 TTLNGKK252 pKa = 8.02 EE253 pKa = 4.06 LSVEE257 pKa = 4.11 VSGSSVTINGEE268 pKa = 4.06 AAVTTADD275 pKa = 3.22 IEE277 pKa = 4.61 GVNGVVHH284 pKa = 7.33 IIDD287 pKa = 3.66 TALAPPSEE295 pKa = 4.32
Molecular weight: 31.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.554
ProMoST 3.91
Dawson 3.757
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.465
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.101
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.884
Patrickios 1.125
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.804
Protein with the highest isoelectric point:
>tr|A0A521B8R2|A0A521B8R2_9BACT Uncharacterized protein OS=Aliifodinibius sediminis OX=1214077 GN=SAMN06265218_102270 PE=4 SV=1
MM1 pKa = 8.07 GDD3 pKa = 3.29 QLSNNGIRR11 pKa = 11.84 SQGKK15 pKa = 6.87 VHH17 pKa = 6.84 PLYY20 pKa = 10.87 FLGLTSAVAILFVLNLSLGSVSIPFPEE47 pKa = 4.98 LLDD50 pKa = 3.26 ILMGGEE56 pKa = 4.16 AGNTAWEE63 pKa = 4.46 KK64 pKa = 10.23 IVINIRR70 pKa = 11.84 LPRR73 pKa = 11.84 AATAVMAGSALAVSGLLMQTLFRR96 pKa = 11.84 NPLAGPSVLGITAGASLGVAVVMLAAGSITTVFAIRR132 pKa = 11.84 QLSVLGSGVIILAASVGSAVVLLLILLISLKK163 pKa = 10.42 VRR165 pKa = 11.84 DD166 pKa = 4.9 NITLLIIGLMVGNMTIALVSIWQYY190 pKa = 9.99 FSRR193 pKa = 11.84 PEE195 pKa = 3.85 QIQDD199 pKa = 3.36 YY200 pKa = 9.66 LIWTFGSLGGVSLGQLWVLGVVTVAGGGIAMVLSKK235 pKa = 10.43 PLNGLLLGKK244 pKa = 10.22 NYY246 pKa = 9.59 ARR248 pKa = 11.84 SMGIPVSRR256 pKa = 11.84 ARR258 pKa = 11.84 LWIIISTSLLAGGITAFCGPIGFVGIAVPHH288 pKa = 6.0 LTRR291 pKa = 11.84 SLLGTNEE298 pKa = 3.79 HH299 pKa = 6.57 RR300 pKa = 11.84 VLIPGTLLMGALLLLACDD318 pKa = 5.51 IIAQMPGSTITLPISAVTSMIGSPVVIWVIIRR350 pKa = 11.84 RR351 pKa = 11.84 KK352 pKa = 8.56 NLKK355 pKa = 9.99 AAFF358 pKa = 3.96
Molecular weight: 37.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.648
IPC_protein 10.35
Toseland 10.555
ProMoST 10.233
Dawson 10.672
Bjellqvist 10.379
Wikipedia 10.877
Rodwell 10.935
Grimsley 10.73
Solomon 10.76
Lehninger 10.73
Nozaki 10.555
DTASelect 10.365
Thurlkill 10.57
EMBOSS 10.95
Sillero 10.599
Patrickios 10.672
IPC_peptide 10.774
IPC2_peptide 9.355
IPC2.peptide.svr19 8.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3822
0
3822
1296162
27
2887
339.1
38.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.291 ± 0.042
0.674 ± 0.012
5.919 ± 0.034
7.346 ± 0.046
4.377 ± 0.03
7.355 ± 0.034
2.184 ± 0.021
6.565 ± 0.036
5.136 ± 0.042
9.492 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.471 ± 0.02
4.366 ± 0.029
4.198 ± 0.024
4.068 ± 0.028
5.374 ± 0.028
6.497 ± 0.029
5.33 ± 0.025
6.321 ± 0.027
1.346 ± 0.015
3.694 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here