Enterococcus faecalis 13-SD-W-01

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; Enterococcus faecalis

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S4D0I8|S4D0I8_ENTFL Rhamnogalacturonan acetylesterase RhgT family protein OS=Enterococcus faecalis 13-SD-W-01 OX=1260356 GN=D920_02190 PE=4 SV=1
MM1 pKa = 7.36NEE3 pKa = 4.34DD4 pKa = 3.39GSVEE8 pKa = 4.09ISWDD12 pKa = 3.63AVDD15 pKa = 4.64GAQAYY20 pKa = 7.82VIRR23 pKa = 11.84YY24 pKa = 9.16GDD26 pKa = 3.84ANQSDD31 pKa = 4.38PHH33 pKa = 4.31QTIYY37 pKa = 10.07MGYY40 pKa = 9.46SEE42 pKa = 4.6TNSWTLAAEE51 pKa = 4.18DD52 pKa = 4.33VPTLEE57 pKa = 5.02EE58 pKa = 3.89GDD60 pKa = 3.91EE61 pKa = 4.22IYY63 pKa = 10.6FYY65 pKa = 11.53VQTFNDD71 pKa = 3.91LGEE74 pKa = 4.3GANDD78 pKa = 3.29IEE80 pKa = 4.94KK81 pKa = 10.68AAFLNEE87 pKa = 4.02NKK89 pKa = 10.38LGSAWSEE96 pKa = 3.74PIILTKK102 pKa = 10.75

Molecular weight: 11.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S4DJE9|S4DJE9_ENTFL EDD domain protein DegV family OS=Enterococcus faecalis 13-SD-W-01 OX=1260356 GN=D920_00370 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84QKK14 pKa = 9.38VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.73GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3105

0

3105

859451

28

2080

276.8

31.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.0 ± 0.047

0.604 ± 0.012

5.16 ± 0.041

7.748 ± 0.054

4.91 ± 0.042

6.379 ± 0.041

1.781 ± 0.019

7.567 ± 0.046

7.119 ± 0.046

9.849 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.621 ± 0.024

4.652 ± 0.034

3.46 ± 0.024

4.152 ± 0.038

3.911 ± 0.033

6.072 ± 0.04

5.842 ± 0.038

6.471 ± 0.04

0.962 ± 0.015

3.74 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski