Amino acid dipepetide frequency for Enterococcus faecalis 13-SD-W-01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.377AlaAla: 6.377 ± 0.108	0.417AlaCys: 0.417 ± 0.024	3.971AlaAsp: 3.971 ± 0.115	5.673AlaGlu: 5.673 ± 0.108	3.282AlaPhe: 3.282 ± 0.067	4.959AlaGly: 4.959 ± 0.091	1.148AlaHis: 1.148 ± 0.039	5.268AlaIle: 5.268 ± 0.098	4.659AlaLys: 4.659 ± 0.088	6.5AlaLeu: 6.5 ± 0.104	1.92AlaMet: 1.92 ± 0.053	2.893AlaAsn: 2.893 ± 0.059	2.089AlaPro: 2.089 ± 0.058	2.706AlaGln: 2.706 ± 0.057	2.452AlaArg: 2.452 ± 0.059	4.15AlaSer: 4.15 ± 0.076	3.235AlaThr: 3.235 ± 0.066	5.289AlaVal: 5.289 ± 0.083	0.552AlaTrp: 0.552 ± 0.028	2.46AlaTyr: 2.46 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.36CysAla: 0.36 ± 0.024	0.085CysCys: 0.085 ± 0.01	0.257CysAsp: 0.257 ± 0.016	0.369CysGlu: 0.369 ± 0.021	0.361CysPhe: 0.361 ± 0.021	0.55CysGly: 0.55 ± 0.026	0.145CysHis: 0.145 ± 0.014	0.42CysIle: 0.42 ± 0.021	0.272CysLys: 0.272 ± 0.019	0.7CysLeu: 0.7 ± 0.03	0.148CysMet: 0.148 ± 0.014	0.208CysAsn: 0.208 ± 0.015	0.248CysPro: 0.248 ± 0.017	0.243CysGln: 0.243 ± 0.015	0.235CysArg: 0.235 ± 0.019	0.407CysSer: 0.407 ± 0.026	0.323CysThr: 0.323 ± 0.02	0.346CysVal: 0.346 ± 0.022	0.073CysTrp: 0.073 ± 0.009	0.287CysTyr: 0.287 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	3.389AspAla: 3.389 ± 0.105	0.284AspCys: 0.284 ± 0.02	2.29AspAsp: 2.29 ± 0.063	4.101AspGlu: 4.101 ± 0.073	2.916AspPhe: 2.916 ± 0.06	3.352AspGly: 3.352 ± 0.063	1.059AspHis: 1.059 ± 0.037	3.751AspIle: 3.751 ± 0.065	3.322AspLys: 3.322 ± 0.073	4.979AspLeu: 4.979 ± 0.077	1.226AspMet: 1.226 ± 0.038	1.97AspAsn: 1.97 ± 0.051	2.116AspPro: 2.116 ± 0.056	2.333AspGln: 2.333 ± 0.054	2.009AspArg: 2.009 ± 0.053	3.068AspSer: 3.068 ± 0.122	2.943AspThr: 2.943 ± 0.064	3.354AspVal: 3.354 ± 0.073	0.635AspTrp: 0.635 ± 0.028	2.498AspTyr: 2.498 ± 0.062	0.0AspXaa: 0.0 ± 0.0
Glu	5.564GluAla: 5.564 ± 0.103	0.306GluCys: 0.306 ± 0.022	3.63GluAsp: 3.63 ± 0.08	7.463GluGlu: 7.463 ± 0.129	2.661GluPhe: 2.661 ± 0.056	3.956GluGly: 3.956 ± 0.073	1.262GluHis: 1.262 ± 0.048	5.841GluIle: 5.841 ± 0.095	7.868GluLys: 7.868 ± 0.115	7.351GluLeu: 7.351 ± 0.112	2.255GluMet: 2.255 ± 0.05	4.562GluAsn: 4.562 ± 0.089	2.007GluPro: 2.007 ± 0.052	3.492GluGln: 3.492 ± 0.08	3.274GluArg: 3.274 ± 0.069	3.508GluSer: 3.508 ± 0.087	4.828GluThr: 4.828 ± 0.085	4.574GluVal: 4.574 ± 0.089	0.719GluTrp: 0.719 ± 0.031	2.356GluTyr: 2.356 ± 0.055	0.0GluXaa: 0.0 ± 0.0
Phe	3.237PheAla: 3.237 ± 0.072	0.436PheCys: 0.436 ± 0.027	2.855PheAsp: 2.855 ± 0.059	3.179PheGlu: 3.179 ± 0.072	3.144PhePhe: 3.144 ± 0.088	3.301PheGly: 3.301 ± 0.077	0.913PheHis: 0.913 ± 0.033	3.586PheIle: 3.586 ± 0.086	2.406PheLys: 2.406 ± 0.061	5.365PheLeu: 5.365 ± 0.126	1.253PheMet: 1.253 ± 0.039	1.878PheAsn: 1.878 ± 0.055	1.708PhePro: 1.708 ± 0.042	1.705PheGln: 1.705 ± 0.047	1.702PheArg: 1.702 ± 0.048	3.877PheSer: 3.877 ± 0.079	2.701PheThr: 2.701 ± 0.054	3.13PheVal: 3.13 ± 0.059	0.563PheTrp: 0.563 ± 0.029	2.161PheTyr: 2.161 ± 0.064	0.0PheXaa: 0.0 ± 0.0
Gly	4.364GlyAla: 4.364 ± 0.095	0.412GlyCys: 0.412 ± 0.023	2.988GlyAsp: 2.988 ± 0.06	4.224GlyGlu: 4.224 ± 0.077	3.299GlyPhe: 3.299 ± 0.071	4.241GlyGly: 4.241 ± 0.087	1.171GlyHis: 1.171 ± 0.032	5.642GlyIle: 5.642 ± 0.095	4.865GlyLys: 4.865 ± 0.089	6.123GlyLeu: 6.123 ± 0.1	1.864GlyMet: 1.864 ± 0.06	2.848GlyAsn: 2.848 ± 0.058	1.395GlyPro: 1.395 ± 0.042	2.267GlyGln: 2.267 ± 0.061	2.412GlyArg: 2.412 ± 0.062	3.797GlySer: 3.797 ± 0.085	4.231GlyThr: 4.231 ± 0.074	4.31GlyVal: 4.31 ± 0.082	0.721GlyTrp: 0.721 ± 0.034	2.617GlyTyr: 2.617 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	1.134HisAla: 1.134 ± 0.03	0.137HisCys: 0.137 ± 0.012	0.826HisAsp: 0.826 ± 0.033	1.212HisGlu: 1.212 ± 0.039	1.124HisPhe: 1.124 ± 0.039	1.174HisGly: 1.174 ± 0.041	0.467HisHis: 0.467 ± 0.024	1.147HisIle: 1.147 ± 0.036	0.869HisLys: 0.869 ± 0.031	1.94HisLeu: 1.94 ± 0.052	0.401HisMet: 0.401 ± 0.021	0.612HisAsn: 0.612 ± 0.025	0.931HisPro: 0.931 ± 0.037	0.848HisGln: 0.848 ± 0.031	0.757HisArg: 0.757 ± 0.032	1.063HisSer: 1.063 ± 0.04	1.005HisThr: 1.005 ± 0.038	1.132HisVal: 1.132 ± 0.036	0.204HisTrp: 0.204 ± 0.016	0.828HisTyr: 0.828 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	5.576IleAla: 5.576 ± 0.087	0.635IleCys: 0.635 ± 0.028	4.528IleAsp: 4.528 ± 0.081	5.391IleGlu: 5.391 ± 0.081	3.73IlePhe: 3.73 ± 0.088	5.231IleGly: 5.231 ± 0.096	1.48IleHis: 1.48 ± 0.043	5.201IleIle: 5.201 ± 0.105	4.508IleLys: 4.508 ± 0.073	7.395IleLeu: 7.395 ± 0.114	1.672IleMet: 1.672 ± 0.044	3.398IleAsn: 3.398 ± 0.07	3.15IlePro: 3.15 ± 0.056	3.176IleGln: 3.176 ± 0.067	3.018IleArg: 3.018 ± 0.063	4.848IleSer: 4.848 ± 0.086	4.413IleThr: 4.413 ± 0.074	4.937IleVal: 4.937 ± 0.079	0.635IleTrp: 0.635 ± 0.027	2.746IleTyr: 2.746 ± 0.065	0.0IleXaa: 0.0 ± 0.0
Lys	4.416LysAla: 4.416 ± 0.076	0.247LysCys: 0.247 ± 0.016	3.574LysAsp: 3.574 ± 0.062	7.79LysGlu: 7.79 ± 0.116	2.039LysPhe: 2.039 ± 0.053	3.819LysGly: 3.819 ± 0.07	1.151LysHis: 1.151 ± 0.04	5.523LysIle: 5.523 ± 0.095	7.455LysLys: 7.455 ± 0.126	5.515LysLeu: 5.515 ± 0.084	2.389LysMet: 2.389 ± 0.053	4.419LysAsn: 4.419 ± 0.079	1.892LysPro: 1.892 ± 0.046	3.265LysGln: 3.265 ± 0.077	3.187LysArg: 3.187 ± 0.066	3.295LysSer: 3.295 ± 0.062	4.145LysThr: 4.145 ± 0.074	4.092LysVal: 4.092 ± 0.082	0.629LysTrp: 0.629 ± 0.029	2.352LysTyr: 2.352 ± 0.061	0.0LysXaa: 0.0 ± 0.0
Leu	7.459LeuAla: 7.459 ± 0.111	0.646LeuCys: 0.646 ± 0.028	5.142LeuAsp: 5.142 ± 0.082	6.567LeuGlu: 6.567 ± 0.105	5.625LeuPhe: 5.625 ± 0.125	6.157LeuGly: 6.157 ± 0.103	1.561LeuHis: 1.561 ± 0.046	7.081LeuIle: 7.081 ± 0.122	6.458LeuLys: 6.458 ± 0.088	10.514LeuLeu: 10.514 ± 0.173	2.475LeuMet: 2.475 ± 0.063	4.382LeuAsn: 4.382 ± 0.078	4.085LeuPro: 4.085 ± 0.075	3.409LeuGln: 3.409 ± 0.079	3.64LeuArg: 3.64 ± 0.072	7.347LeuSer: 7.347 ± 0.109	6.096LeuThr: 6.096 ± 0.084	6.055LeuVal: 6.055 ± 0.095	0.738LeuTrp: 0.738 ± 0.03	3.057LeuTyr: 3.057 ± 0.074	0.0LeuXaa: 0.0 ± 0.0
Met	1.807MetAla: 1.807 ± 0.054	0.126MetCys: 0.126 ± 0.012	1.477MetAsp: 1.477 ± 0.046	1.831MetGlu: 1.831 ± 0.047	1.024MetPhe: 1.024 ± 0.038	1.578MetGly: 1.578 ± 0.052	0.369MetHis: 0.369 ± 0.02	2.298MetIle: 2.298 ± 0.059	2.364MetLys: 2.364 ± 0.052	2.382MetLeu: 2.382 ± 0.057	0.842MetMet: 0.842 ± 0.036	1.538MetAsn: 1.538 ± 0.043	0.967MetPro: 0.967 ± 0.031	0.924MetGln: 0.924 ± 0.033	1.055MetArg: 1.055 ± 0.034	1.504MetSer: 1.504 ± 0.041	1.648MetThr: 1.648 ± 0.045	1.563MetVal: 1.563 ± 0.047	0.149MetTrp: 0.149 ± 0.014	0.768MetTyr: 0.768 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	2.832AsnAla: 2.832 ± 0.06	0.308AsnCys: 0.308 ± 0.017	2.468AsnAsp: 2.468 ± 0.068	3.479AsnGlu: 3.479 ± 0.062	2.008AsnPhe: 2.008 ± 0.051	3.43AsnGly: 3.43 ± 0.066	1.102AsnHis: 1.102 ± 0.034	3.244AsnIle: 3.244 ± 0.065	3.061AsnLys: 3.061 ± 0.07	4.192AsnLeu: 4.192 ± 0.066	1.137AsnMet: 1.137 ± 0.034	2.315AsnAsn: 2.315 ± 0.065	2.023AsnPro: 2.023 ± 0.048	2.449AsnGln: 2.449 ± 0.061	1.976AsnArg: 1.976 ± 0.053	2.496AsnSer: 2.496 ± 0.062	2.456AsnThr: 2.456 ± 0.068	2.95AsnVal: 2.95 ± 0.068	0.555AsnTrp: 0.555 ± 0.027	2.035AsnTyr: 2.035 ± 0.054	0.0AsnXaa: 0.0 ± 0.0
Pro	2.511ProAla: 2.511 ± 0.057	0.157ProCys: 0.157 ± 0.013	1.985ProAsp: 1.985 ± 0.046	3.551ProGlu: 3.551 ± 0.072	1.848ProPhe: 1.848 ± 0.052	1.954ProGly: 1.954 ± 0.058	0.574ProHis: 0.574 ± 0.028	2.613ProIle: 2.613 ± 0.06	2.229ProLys: 2.229 ± 0.051	3.24ProLeu: 3.24 ± 0.068	0.812ProMet: 0.812 ± 0.032	1.645ProAsn: 1.645 ± 0.042	0.742ProPro: 0.742 ± 0.034	1.253ProGln: 1.253 ± 0.04	1.089ProArg: 1.089 ± 0.037	2.026ProSer: 2.026 ± 0.049	2.098ProThr: 2.098 ± 0.056	2.659ProVal: 2.659 ± 0.063	0.319ProTrp: 0.319 ± 0.02	1.299ProTyr: 1.299 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	3.208GlnAla: 3.208 ± 0.063	0.149GlnCys: 0.149 ± 0.013	1.622GlnAsp: 1.622 ± 0.044	3.69GlnGlu: 3.69 ± 0.078	1.593GlnPhe: 1.593 ± 0.044	2.171GlnGly: 2.171 ± 0.046	0.645GlnHis: 0.645 ± 0.024	2.92GlnIle: 2.92 ± 0.059	3.539GlnLys: 3.539 ± 0.073	4.688GlnLeu: 4.688 ± 0.087	1.155GlnMet: 1.155 ± 0.038	1.865GlnAsn: 1.865 ± 0.054	1.411GlnPro: 1.411 ± 0.04	2.257GlnGln: 2.257 ± 0.076	1.729GlnArg: 1.729 ± 0.044	2.143GlnSer: 2.143 ± 0.051	2.642GlnThr: 2.642 ± 0.064	2.443GlnVal: 2.443 ± 0.052	0.414GlnTrp: 0.414 ± 0.023	1.231GlnTyr: 1.231 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.172ArgAla: 2.172 ± 0.055	0.233ArgCys: 0.233 ± 0.016	1.748ArgAsp: 1.748 ± 0.048	2.967ArgGlu: 2.967 ± 0.067	2.139ArgPhe: 2.139 ± 0.055	2.155ArgGly: 2.155 ± 0.057	0.705ArgHis: 0.705 ± 0.035	3.078ArgIle: 3.078 ± 0.06	3.347ArgLys: 3.347 ± 0.067	3.987ArgLeu: 3.987 ± 0.07	1.127ArgMet: 1.127 ± 0.035	1.847ArgAsn: 1.847 ± 0.046	1.316ArgPro: 1.316 ± 0.035	1.78ArgGln: 1.78 ± 0.048	1.779ArgArg: 1.779 ± 0.046	2.205ArgSer: 2.205 ± 0.057	2.037ArgThr: 2.037 ± 0.052	2.406ArgVal: 2.406 ± 0.057	0.378ArgTrp: 0.378 ± 0.021	1.708ArgTyr: 1.708 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	3.987SerAla: 3.987 ± 0.075	0.339SerCys: 0.339 ± 0.02	3.103SerAsp: 3.103 ± 0.101	4.362SerGlu: 4.362 ± 0.095	3.449SerPhe: 3.449 ± 0.069	4.519SerGly: 4.519 ± 0.075	0.975SerHis: 0.975 ± 0.034	4.7SerIle: 4.7 ± 0.07	3.698SerLys: 3.698 ± 0.081	6.273SerLeu: 6.273 ± 0.11	1.523SerMet: 1.523 ± 0.047	2.702SerAsn: 2.702 ± 0.068	1.869SerPro: 1.869 ± 0.051	2.237SerGln: 2.237 ± 0.053	2.362SerArg: 2.362 ± 0.055	4.071SerSer: 4.071 ± 0.094	3.275SerThr: 3.275 ± 0.073	4.064SerVal: 4.064 ± 0.075	0.703SerTrp: 0.703 ± 0.033	2.507SerTyr: 2.507 ± 0.057	0.0SerXaa: 0.0 ± 0.0
Thr	4.381ThrAla: 4.381 ± 0.079	0.304ThrCys: 0.304 ± 0.021	3.052ThrAsp: 3.052 ± 0.065	4.148ThrGlu: 4.148 ± 0.077	2.751ThrPhe: 2.751 ± 0.051	4.132ThrGly: 4.132 ± 0.078	1.029ThrHis: 1.029 ± 0.036	5.086ThrIle: 5.086 ± 0.074	3.642ThrLys: 3.642 ± 0.07	5.371ThrLeu: 5.371 ± 0.084	1.343ThrMet: 1.343 ± 0.039	2.762ThrAsn: 2.762 ± 0.062	2.399ThrPro: 2.399 ± 0.055	1.958ThrGln: 1.958 ± 0.055	1.886ThrArg: 1.886 ± 0.041	3.627ThrSer: 3.627 ± 0.066	3.627ThrThr: 3.627 ± 0.098	4.264ThrVal: 4.264 ± 0.077	0.533ThrTrp: 0.533 ± 0.027	2.128ThrTyr: 2.128 ± 0.06	0.0ThrXaa: 0.0 ± 0.0
Val	4.394ValAla: 4.394 ± 0.084	0.472ValCys: 0.472 ± 0.021	3.539ValAsp: 3.539 ± 0.079	4.293ValGlu: 4.293 ± 0.091	3.289ValPhe: 3.289 ± 0.068	4.103ValGly: 4.103 ± 0.077	1.06ValHis: 1.06 ± 0.033	5.211ValIle: 5.211 ± 0.086	4.104ValLys: 4.104 ± 0.082	6.587ValLeu: 6.587 ± 0.088	1.642ValMet: 1.642 ± 0.044	2.708ValAsn: 2.708 ± 0.057	2.585ValPro: 2.585 ± 0.063	2.411ValGln: 2.411 ± 0.053	2.375ValArg: 2.375 ± 0.056	4.499ValSer: 4.499 ± 0.08	4.015ValThr: 4.015 ± 0.074	4.497ValVal: 4.497 ± 0.083	0.55ValTrp: 0.55 ± 0.028	2.371ValTyr: 2.371 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	0.558TrpAla: 0.558 ± 0.027	0.069TrpCys: 0.069 ± 0.009	0.47TrpAsp: 0.47 ± 0.027	0.646TrpGlu: 0.646 ± 0.025	0.54TrpPhe: 0.54 ± 0.028	0.639TrpGly: 0.639 ± 0.028	0.183TrpHis: 0.183 ± 0.014	0.704TrpIle: 0.704 ± 0.03	0.609TrpLys: 0.609 ± 0.027	1.153TrpLeu: 1.153 ± 0.037	0.263TrpMet: 0.263 ± 0.018	0.49TrpAsn: 0.49 ± 0.028	0.214TrpPro: 0.214 ± 0.014	0.532TrpGln: 0.532 ± 0.028	0.444TrpArg: 0.444 ± 0.018	0.603TrpSer: 0.603 ± 0.025	0.532TrpThr: 0.532 ± 0.027	0.527TrpVal: 0.527 ± 0.027	0.126TrpTrp: 0.126 ± 0.013	0.325TrpTyr: 0.325 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.272TyrAla: 2.272 ± 0.055	0.278TyrCys: 0.278 ± 0.02	2.071TyrAsp: 2.071 ± 0.06	2.541TyrGlu: 2.541 ± 0.054	2.281TyrPhe: 2.281 ± 0.065	2.368TyrGly: 2.368 ± 0.056	0.816TyrHis: 0.816 ± 0.033	2.354TyrIle: 2.354 ± 0.06	1.918TyrLys: 1.918 ± 0.052	4.238TyrLeu: 4.238 ± 0.084	0.764TyrMet: 0.764 ± 0.034	1.475TyrAsn: 1.475 ± 0.046	1.457TyrPro: 1.457 ± 0.044	2.271TyrGln: 2.271 ± 0.053	1.733TyrArg: 1.733 ± 0.045	2.183TyrSer: 2.183 ± 0.065	2.172TyrThr: 2.172 ± 0.059	2.114TyrVal: 2.114 ± 0.047	0.428TyrTrp: 0.428 ± 0.023	1.663TyrTyr: 1.663 ± 0.051	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3105 proteins (859451 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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