Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanosarcina; Methanosarcina mazei

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3303 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8PVK2|Q8PVK2_METMA Uncharacterized protein OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) OX=192952 GN=MM_1961 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.36KK3 pKa = 10.24SIALISVFAVLMIAFSGCVDD23 pKa = 3.71NSPAVNGTDD32 pKa = 3.28SDD34 pKa = 4.07NPEE37 pKa = 4.23TNPISDD43 pKa = 4.33DD44 pKa = 3.6DD45 pKa = 4.23AAGVSTIEE53 pKa = 3.97NPPAGFEE60 pKa = 4.35YY61 pKa = 10.07IDD63 pKa = 4.65TIPLSTDD70 pKa = 3.51EE71 pKa = 4.55IKK73 pKa = 9.84VTYY76 pKa = 9.47EE77 pKa = 3.78AEE79 pKa = 4.15NVTGILSGSEE89 pKa = 3.82GIYY92 pKa = 9.92RR93 pKa = 11.84DD94 pKa = 3.89SNNTDD99 pKa = 3.41YY100 pKa = 11.41YY101 pKa = 11.01VDD103 pKa = 4.63AIEE106 pKa = 5.45LEE108 pKa = 4.2NEE110 pKa = 3.65EE111 pKa = 4.43AANNLIDD118 pKa = 4.56AYY120 pKa = 10.48RR121 pKa = 11.84SSFPPLSDD129 pKa = 3.08GSRR132 pKa = 11.84FTEE135 pKa = 4.33VSFNGHH141 pKa = 6.07SGVKK145 pKa = 7.67ITDD148 pKa = 3.94YY149 pKa = 9.71ITSGGKK155 pKa = 8.91SVPRR159 pKa = 11.84YY160 pKa = 9.97SYY162 pKa = 9.66IWSNEE167 pKa = 3.42NYY169 pKa = 10.18VIVVFGNTAEE179 pKa = 4.12EE180 pKa = 4.28APVRR184 pKa = 11.84QLAEE188 pKa = 3.84ATGYY192 pKa = 10.63

Molecular weight: 20.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8PYR5|Q8PYR5_METMA Inhibitor_I42 domain-containing protein OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) OX=192952 GN=MM_0795 PE=4 SV=1
MM1 pKa = 6.8SHH3 pKa = 6.05NMKK6 pKa = 10.11GQKK9 pKa = 9.42KK10 pKa = 9.38RR11 pKa = 11.84LAKK14 pKa = 10.02AHH16 pKa = 5.94KK17 pKa = 8.8QNSRR21 pKa = 11.84VPVWAIVKK29 pKa = 7.23TNRR32 pKa = 11.84KK33 pKa = 8.7VVSHH37 pKa = 6.09PRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 3.61WRR44 pKa = 11.84RR45 pKa = 11.84GSLDD49 pKa = 3.28VKK51 pKa = 11.03

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3303

0

3303

1004248

16

1898

304.0

33.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.103 ± 0.05

1.236 ± 0.021

5.26 ± 0.03

8.16 ± 0.057

4.296 ± 0.04

7.266 ± 0.044

1.665 ± 0.017

7.656 ± 0.035

6.771 ± 0.043

9.387 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.023

4.339 ± 0.046

4.009 ± 0.027

2.468 ± 0.023

4.652 ± 0.037

6.768 ± 0.046

5.116 ± 0.038

6.894 ± 0.04

0.936 ± 0.014

3.531 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski