Halobacterium sp. DL1

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Halobacterium; unclassified Halobacterium

Average proteome isoelectric point is 4.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3265 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0K6I1|W0K6I1_9EURY Uncharacterized protein OS=Halobacterium sp. DL1 OX=751944 GN=HALDL1_04040 PE=4 SV=1
MM1 pKa = 7.68AEE3 pKa = 3.99YY4 pKa = 10.95ANDD7 pKa = 3.72VLVTADD13 pKa = 3.32WVEE16 pKa = 3.99EE17 pKa = 3.87NLDD20 pKa = 3.62QFEE23 pKa = 4.95SDD25 pKa = 3.68DD26 pKa = 3.56PDD28 pKa = 3.53YY29 pKa = 11.42RR30 pKa = 11.84LVEE33 pKa = 3.99VDD35 pKa = 4.33VDD37 pKa = 3.7TEE39 pKa = 4.45AYY41 pKa = 10.36DD42 pKa = 3.99DD43 pKa = 3.7AHH45 pKa = 7.27APGAIGFNWEE55 pKa = 4.26TQLQDD60 pKa = 3.04QTQRR64 pKa = 11.84DD65 pKa = 3.84ILEE68 pKa = 4.44KK69 pKa = 10.44QDD71 pKa = 3.74FADD74 pKa = 3.93LLGSHH79 pKa = 7.47GITEE83 pKa = 4.72DD84 pKa = 3.63STVVLYY90 pKa = 11.09GDD92 pKa = 3.44NSNWFAAYY100 pKa = 8.45TYY102 pKa = 9.6WQFKK106 pKa = 10.18YY107 pKa = 10.56YY108 pKa = 10.54GHH110 pKa = 7.23EE111 pKa = 4.08DD112 pKa = 3.33VKK114 pKa = 11.32LLDD117 pKa = 3.69GGRR120 pKa = 11.84DD121 pKa = 3.4YY122 pKa = 10.71WVEE125 pKa = 3.55NDD127 pKa = 3.85YY128 pKa = 8.71PTTDD132 pKa = 3.46EE133 pKa = 4.77APSFSEE139 pKa = 3.58QEE141 pKa = 4.0YY142 pKa = 9.46NARR145 pKa = 11.84GPFDD149 pKa = 4.84GIRR152 pKa = 11.84AYY154 pKa = 10.78RR155 pKa = 11.84DD156 pKa = 3.5DD157 pKa = 4.0VEE159 pKa = 4.12NAIDD163 pKa = 3.77RR164 pKa = 11.84GIPLVDD170 pKa = 3.21VRR172 pKa = 11.84SPEE175 pKa = 4.06EE176 pKa = 3.62FSGEE180 pKa = 3.6ILAPPGLQEE189 pKa = 3.56TAQRR193 pKa = 11.84GGHH196 pKa = 6.15IPGASNISWAATVNDD211 pKa = 4.04DD212 pKa = 4.45GTFKK216 pKa = 10.92SHH218 pKa = 8.23DD219 pKa = 3.99EE220 pKa = 4.08LQDD223 pKa = 3.54LYY225 pKa = 11.31EE226 pKa = 5.86DD227 pKa = 4.07GGITNDD233 pKa = 3.6QEE235 pKa = 4.17VVAYY239 pKa = 9.79CRR241 pKa = 11.84IGEE244 pKa = 4.16RR245 pKa = 11.84SSIAWFALSEE255 pKa = 4.05LLGYY259 pKa = 10.94DD260 pKa = 4.48DD261 pKa = 3.57VTNYY265 pKa = 10.53DD266 pKa = 3.73GSWTEE271 pKa = 3.68WGNLVDD277 pKa = 5.76APIEE281 pKa = 4.19TGAGDD286 pKa = 3.77DD287 pKa = 3.59

Molecular weight: 32.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0K2Q1|W0K2Q1_9EURY Uncharacterized protein OS=Halobacterium sp. DL1 OX=751944 GN=HALDL1_07970 PE=4 SV=1
MM1 pKa = 6.92YY2 pKa = 10.23RR3 pKa = 11.84SLRR6 pKa = 11.84DD7 pKa = 2.95AATTVGSVAVLLVVVMLVSSDD28 pKa = 3.32TKK30 pKa = 10.27TVVALMVFCVVGWVFSRR47 pKa = 11.84AYY49 pKa = 10.01RR50 pKa = 11.84SSSTVGAVRR59 pKa = 11.84ARR61 pKa = 11.84LSPRR65 pKa = 11.84AKK67 pKa = 9.55RR68 pKa = 11.84AIGVVVLIGVVIVVADD84 pKa = 3.91EE85 pKa = 4.15LDD87 pKa = 3.83EE88 pKa = 4.43VVVLAVAGLFGYY100 pKa = 9.62VGYY103 pKa = 10.27RR104 pKa = 11.84AGSVLRR110 pKa = 11.84GAWHH114 pKa = 6.03GTLGGILGSVCLVVLLGVMFLLATVVGVDD143 pKa = 3.69GARR146 pKa = 11.84TIALKK151 pKa = 9.76TLVAPQVVGFVTLVWSVFVVVLGGVVGLISGSVGGAFARR190 pKa = 11.84LFF192 pKa = 3.76

Molecular weight: 19.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3265

0

3265

904200

29

2012

276.9

30.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.174 ± 0.076

0.694 ± 0.014

8.519 ± 0.055

8.596 ± 0.067

3.461 ± 0.031

8.435 ± 0.047

2.088 ± 0.022

3.375 ± 0.037

1.864 ± 0.029

8.97 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.732 ± 0.018

2.368 ± 0.024

4.555 ± 0.029

2.63 ± 0.025

6.426 ± 0.041

5.382 ± 0.031

6.157 ± 0.041

9.727 ± 0.06

1.119 ± 0.016

2.728 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski