Lutibacter oricola
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3464 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H3G2N9|A0A1H3G2N9_9FLAO Uncharacterized protein OS=Lutibacter oricola OX=762486 GN=SAMN05444411_11311 PE=4 SV=1
MM1 pKa = 7.21 KK2 pKa = 10.09 QIKK5 pKa = 9.53 YY6 pKa = 9.77 LISAIFLFAAVWSCTEE22 pKa = 4.39 DD23 pKa = 3.34 EE24 pKa = 4.98 LGNADD29 pKa = 4.07 FVDD32 pKa = 4.66 TIVAPTEE39 pKa = 3.78 VSAMFNVTQDD49 pKa = 3.05 NTGLVTIAPTSQGAVTYY66 pKa = 9.59 TVDD69 pKa = 4.72 LGDD72 pKa = 4.04 GSDD75 pKa = 4.98 PITLKK80 pKa = 10.61 QGEE83 pKa = 4.53 SIKK86 pKa = 11.08 NIFAEE91 pKa = 4.36 GEE93 pKa = 4.29 YY94 pKa = 9.97 QVKK97 pKa = 8.32 ITAIGVTGLTTSVTLPLVVSFKK119 pKa = 10.96 APEE122 pKa = 3.95 NLVVEE127 pKa = 4.58 ILNDD131 pKa = 3.52 EE132 pKa = 4.38 AVSKK136 pKa = 10.12 QVNVTATADD145 pKa = 3.34 NAINFDD151 pKa = 3.71 VYY153 pKa = 10.91 FGVEE157 pKa = 4.42 GVTEE161 pKa = 5.45 PITGVIGEE169 pKa = 4.6 TVSYY173 pKa = 9.98 VYY175 pKa = 9.76 PEE177 pKa = 4.0 PGIYY181 pKa = 8.99 TIKK184 pKa = 10.68 VVAKK188 pKa = 9.34 GAAIEE193 pKa = 4.1 TTEE196 pKa = 4.11 YY197 pKa = 10.41 IEE199 pKa = 4.13 EE200 pKa = 4.37 FEE202 pKa = 4.27 VTEE205 pKa = 4.19 IEE207 pKa = 4.36 EE208 pKa = 4.44 PLEE211 pKa = 4.26 KK212 pKa = 10.72 APTPPYY218 pKa = 9.94 RR219 pKa = 11.84 NEE221 pKa = 3.7 VDD223 pKa = 3.72 VVSIFSDD230 pKa = 3.6 AYY232 pKa = 9.68 TNITVNEE239 pKa = 4.18 WNPGWGQSTTLTDD252 pKa = 4.11 FEE254 pKa = 4.81 VDD256 pKa = 2.88 GDD258 pKa = 4.38 NILKK262 pKa = 10.3 YY263 pKa = 10.93 DD264 pKa = 3.88 FLNYY268 pKa = 9.57 TGIVTDD274 pKa = 4.05 YY275 pKa = 11.6 DD276 pKa = 3.71 NPTNLAQMEE285 pKa = 4.6 FVHH288 pKa = 6.97 FDD290 pKa = 2.94 YY291 pKa = 8.2 WTNDD295 pKa = 3.26 AEE297 pKa = 4.41 SLSLKK302 pKa = 10.02 IVNTSQADD310 pKa = 3.68 GTPEE314 pKa = 4.0 KK315 pKa = 10.18 EE316 pKa = 4.03 SEE318 pKa = 4.14 IAVGDD323 pKa = 3.53 ITAGEE328 pKa = 4.24 WVSVEE333 pKa = 3.96 IPLSDD338 pKa = 3.43 FTTNMSGVTQFLFVSNGVTVFIDD361 pKa = 2.92 NFYY364 pKa = 10.58 FYY366 pKa = 10.54 KK367 pKa = 10.6 QPTTVSEE374 pKa = 4.74 GIVGVWKK381 pKa = 9.96 LAPIAGALKK390 pKa = 10.55 VGPNPNDD397 pKa = 3.61 GSWWANSAEE406 pKa = 4.28 DD407 pKa = 3.64 VTGRR411 pKa = 11.84 DD412 pKa = 3.72 CLFDD416 pKa = 4.1 DD417 pKa = 4.87 SYY419 pKa = 12.23 VFGADD424 pKa = 3.15 GTFKK428 pKa = 11.11 NILDD432 pKa = 3.73 GSTWVEE438 pKa = 4.16 GWQGGSDD445 pKa = 3.49 SCATPVAPHH454 pKa = 7.12 DD455 pKa = 4.31 GSNPATFVYY464 pKa = 10.57 DD465 pKa = 3.86 EE466 pKa = 4.64 AAGKK470 pKa = 8.14 VTLNGLGAYY479 pKa = 10.31 LGIPKK484 pKa = 10.13 AYY486 pKa = 10.23 DD487 pKa = 3.39 GGEE490 pKa = 4.1 LGSPADD496 pKa = 3.88 APSAINYY503 pKa = 8.19 DD504 pKa = 3.54 VTLSNNDD511 pKa = 2.84 SAMTVVVNIGSGYY524 pKa = 6.42 WTFEE528 pKa = 4.0 LVKK531 pKa = 10.44 DD532 pKa = 4.14 GAPAYY537 pKa = 10.64 SPLEE541 pKa = 4.18 GTWVVAPEE549 pKa = 4.17 AGSLGVGPGQGDD561 pKa = 3.95 TSWWTIDD568 pKa = 3.33 AAGVTQRR575 pKa = 11.84 ACFFDD580 pKa = 3.59 DD581 pKa = 3.65 TFVFGADD588 pKa = 3.13 GSFMNVLGADD598 pKa = 3.29 TWIEE602 pKa = 4.03 GWQGGTDD609 pKa = 3.19 ACGVPVAPHH618 pKa = 7.12 DD619 pKa = 4.08 GMNPATYY626 pKa = 8.88 TYY628 pKa = 11.11 DD629 pKa = 3.43 EE630 pKa = 4.61 AAGKK634 pKa = 8.45 LTLNGIGAYY643 pKa = 10.34 LGIPKK648 pKa = 10.01 AYY650 pKa = 10.5 NEE652 pKa = 4.74 GEE654 pKa = 4.16 LASPADD660 pKa = 3.83 APASITYY667 pKa = 10.43 DD668 pKa = 3.18 VTLANNDD675 pKa = 3.09 SEE677 pKa = 4.61 MTVDD681 pKa = 3.02 IWVGGDD687 pKa = 2.88 AWWRR691 pKa = 11.84 FKK693 pKa = 9.91 MVKK696 pKa = 9.95 EE697 pKa = 4.35 GTSTGGGSGTGGGTSSGTQIDD718 pKa = 4.35 LPVDD722 pKa = 4.38 FEE724 pKa = 4.7 SATIDD729 pKa = 3.37 YY730 pKa = 8.23 TLTDD734 pKa = 3.71 FGEE737 pKa = 4.35 NLSSIVEE744 pKa = 4.3 DD745 pKa = 3.57 PTNASNTVAKK755 pKa = 10.16 VVKK758 pKa = 8.93 TAAATTWAGTTIGTDD773 pKa = 2.73 TGFATNIPISLTNSIITVRR792 pKa = 11.84 VWSPDD797 pKa = 2.49 AGAPIRR803 pKa = 11.84 LKK805 pKa = 10.58 IEE807 pKa = 4.3 DD808 pKa = 4.59 ANDD811 pKa = 3.39 ATHH814 pKa = 5.87 TCEE817 pKa = 4.24 TEE819 pKa = 4.06 VNTTVAGGWEE829 pKa = 4.17 TIEE832 pKa = 5.57 FDD834 pKa = 4.13 FTNQATGTEE843 pKa = 4.23 LLSVGLDD850 pKa = 3.27 NGWVYY855 pKa = 11.43 NKK857 pKa = 10.7 ASIFFNFGTDD867 pKa = 3.04 GATAGEE873 pKa = 4.0 KK874 pKa = 9.59 TYY876 pKa = 11.21 YY877 pKa = 10.01 FDD879 pKa = 6.31 DD880 pKa = 4.63 VIFINNN886 pKa = 3.32
Molecular weight: 94.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.719
IPC_protein 3.757
Toseland 3.528
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.643
Rodwell 3.579
Grimsley 3.439
Solomon 3.732
Lehninger 3.694
Nozaki 3.834
DTASelect 4.062
Thurlkill 3.579
EMBOSS 3.656
Sillero 3.872
Patrickios 1.265
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.766
Protein with the highest isoelectric point:
>tr|A0A1H2Z930|A0A1H2Z930_9FLAO Nitroreductase OS=Lutibacter oricola OX=762486 GN=SAMN05444411_103179 PE=4 SV=1
MM1 pKa = 7.54 SVRR4 pKa = 11.84 KK5 pKa = 9.68 LKK7 pKa = 10.5 PITPGQRR14 pKa = 11.84 FRR16 pKa = 11.84 VVNGFDD22 pKa = 5.49 AITTDD27 pKa = 3.56 KK28 pKa = 11.07 PEE30 pKa = 4.39 KK31 pKa = 10.55 SLLAPKK37 pKa = 9.91 KK38 pKa = 10.38 RR39 pKa = 11.84 SGGRR43 pKa = 11.84 NNKK46 pKa = 9.29 GKK48 pKa = 8.29 MTMRR52 pKa = 11.84 YY53 pKa = 9.26 IGGGHH58 pKa = 6.45 KK59 pKa = 9.63 KK60 pKa = 9.44 HH61 pKa = 6.1 YY62 pKa = 10.08 RR63 pKa = 11.84 IIDD66 pKa = 3.83 FKK68 pKa = 10.83 RR69 pKa = 11.84 NKK71 pKa = 9.58 HH72 pKa = 5.09 GVPATVKK79 pKa = 10.58 SIEE82 pKa = 4.0 YY83 pKa = 10.15 DD84 pKa = 3.43 PNRR87 pKa = 11.84 TAFIALLNYY96 pKa = 10.38 ADD98 pKa = 3.65 GEE100 pKa = 4.2 KK101 pKa = 10.21 RR102 pKa = 11.84 YY103 pKa = 10.74 VIAQNGLKK111 pKa = 10.38 AGQTVISGEE120 pKa = 4.13 GATPDD125 pKa = 3.47 VGNTMLLSEE134 pKa = 4.99 IPLGTTISCIEE145 pKa = 3.88 LHH147 pKa = 6.73 PGQGAVIARR156 pKa = 11.84 SAGSFAQLMARR167 pKa = 11.84 EE168 pKa = 4.45 GKK170 pKa = 10.09 YY171 pKa = 9.05 ATIKK175 pKa = 10.52 LPSGEE180 pKa = 4.19 TRR182 pKa = 11.84 LILLTCSATIGVVSNSDD199 pKa = 3.21 HH200 pKa = 6.09 QLLVSGKK207 pKa = 9.87 AGRR210 pKa = 11.84 TRR212 pKa = 11.84 WLGRR216 pKa = 11.84 RR217 pKa = 11.84 PRR219 pKa = 11.84 TRR221 pKa = 11.84 PVVMNPVDD229 pKa = 3.47 HH230 pKa = 7.05 PMGGGEE236 pKa = 4.28 GKK238 pKa = 10.51 SSGGHH243 pKa = 4.59 PRR245 pKa = 11.84 SRR247 pKa = 11.84 NGIPAKK253 pKa = 10.41 GFKK256 pKa = 9.24 TRR258 pKa = 11.84 SKK260 pKa = 9.37 TKK262 pKa = 10.4 ASNKK266 pKa = 9.95 YY267 pKa = 9.56 IIEE270 pKa = 4.06 RR271 pKa = 11.84 RR272 pKa = 11.84 KK273 pKa = 10.1 KK274 pKa = 9.82
Molecular weight: 29.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.303
IPC2_protein 9.897
IPC_protein 10.584
Toseland 10.994
ProMoST 10.657
Dawson 11.052
Bjellqvist 10.73
Wikipedia 11.242
Rodwell 11.403
Grimsley 11.082
Solomon 11.184
Lehninger 11.155
Nozaki 10.965
DTASelect 10.73
Thurlkill 10.965
EMBOSS 11.389
Sillero 10.979
Patrickios 11.111
IPC_peptide 11.184
IPC2_peptide 9.502
IPC2.peptide.svr19 8.553
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3464
0
3464
1260383
25
6593
363.9
41.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.891 ± 0.042
0.772 ± 0.014
5.279 ± 0.037
6.73 ± 0.043
5.208 ± 0.034
6.35 ± 0.038
1.657 ± 0.019
8.206 ± 0.039
8.719 ± 0.069
8.936 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.068 ± 0.022
6.929 ± 0.046
3.25 ± 0.026
2.974 ± 0.021
2.979 ± 0.024
6.61 ± 0.036
5.852 ± 0.059
6.324 ± 0.04
1.132 ± 0.016
4.134 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here