Adelphocoris suturalis virus
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L6YQ04|A0A1L6YQ04_9VIRU ORF4 OS=Adelphocoris suturalis virus OX=1930920 PE=4 SV=1
MM1 pKa = 7.54 AAALLEE7 pKa = 4.29 AAEE10 pKa = 4.15 IAAEE14 pKa = 4.18 IIEE17 pKa = 4.22 AAKK20 pKa = 10.33 LAEE23 pKa = 4.03 EE24 pKa = 5.05 LGTGAYY30 pKa = 10.26 DD31 pKa = 4.17 LATSAYY37 pKa = 9.27 RR38 pKa = 11.84 GLKK41 pKa = 10.15 RR42 pKa = 11.84 IFGTVTQRR50 pKa = 11.84 YY51 pKa = 8.38 EE52 pKa = 3.67 PVTNFRR58 pKa = 11.84 FARR61 pKa = 11.84 SFTFIEE67 pKa = 4.34 LPRR70 pKa = 11.84 LQRR73 pKa = 11.84 VLLRR77 pKa = 11.84 MQNINYY83 pKa = 9.02 SLYY86 pKa = 10.49 YY87 pKa = 8.16 EE88 pKa = 4.17 RR89 pKa = 11.84 EE90 pKa = 4.04 YY91 pKa = 11.51 ARR93 pKa = 11.84 SLVSSITHH101 pKa = 5.42 DD102 pKa = 2.77 HH103 pKa = 6.17 SMNFFVDD110 pKa = 5.08 GIFRR114 pKa = 11.84 EE115 pKa = 4.3 DD116 pKa = 3.48 VVVVIISRR124 pKa = 11.84 QPFLIPFSLLSRR136 pKa = 11.84 SLSLSQHH143 pKa = 5.3 PTNDD147 pKa = 2.83 EE148 pKa = 4.11 LTTHH152 pKa = 6.94 RR153 pKa = 11.84 EE154 pKa = 3.71 LYY156 pKa = 8.41 YY157 pKa = 10.75 TSTNNLLALVNDD169 pKa = 4.48 RR170 pKa = 11.84 SNIYY174 pKa = 10.23 DD175 pKa = 3.43 KK176 pKa = 11.36 QKK178 pKa = 10.76 FEE180 pKa = 5.04 HH181 pKa = 6.75 EE182 pKa = 4.27 FDD184 pKa = 3.6 LKK186 pKa = 10.86 IVVDD190 pKa = 4.04
Molecular weight: 21.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.555
IPC2_protein 5.563
IPC_protein 5.537
Toseland 5.919
ProMoST 5.601
Dawson 5.715
Bjellqvist 5.753
Wikipedia 5.677
Rodwell 5.677
Grimsley 6.097
Solomon 5.715
Lehninger 5.69
Nozaki 5.931
DTASelect 6.109
Thurlkill 6.059
EMBOSS 6.02
Sillero 6.033
Patrickios 4.101
IPC_peptide 5.728
IPC2_peptide 6.046
IPC2.peptide.svr19 6.023
Protein with the highest isoelectric point:
>tr|A0A1L6YQ03|A0A1L6YQ03_9VIRU ORF2 OS=Adelphocoris suturalis virus OX=1930920 PE=4 SV=1
MM1 pKa = 7.33 FCLMMLMAGIIIHH14 pKa = 6.83 KK15 pKa = 9.62 SMGMRR20 pKa = 11.84 SMSLPAIVHH29 pKa = 5.47 ATEE32 pKa = 3.87 STPAVVYY39 pKa = 10.87 SSDD42 pKa = 3.56 SDD44 pKa = 3.95 SVSIITDD51 pKa = 3.65 DD52 pKa = 4.39 DD53 pKa = 4.05 LVDD56 pKa = 3.51 YY57 pKa = 11.11 SVVQRR62 pKa = 11.84 RR63 pKa = 11.84 SRR65 pKa = 11.84 RR66 pKa = 11.84 APPPEE71 pKa = 4.27 RR72 pKa = 11.84 PCSGSYY78 pKa = 10.16 YY79 pKa = 10.88 DD80 pKa = 3.87 NGTWKK85 pKa = 8.89 YY86 pKa = 7.89 TQDD89 pKa = 3.62 SCNVLADD96 pKa = 3.95 RR97 pKa = 11.84 CQVLNNGAPCSVKK110 pKa = 10.58 CGTSSSRR117 pKa = 11.84 TKK119 pKa = 10.55 RR120 pKa = 11.84 QTKK123 pKa = 6.17 YY124 pKa = 9.08 TPIVHH129 pKa = 6.35 TPPRR133 pKa = 11.84 QPVVTHH139 pKa = 6.33 KK140 pKa = 11.22 GKK142 pKa = 11.07 VNVSADD148 pKa = 3.04 TDD150 pKa = 3.35 IYY152 pKa = 11.32 YY153 pKa = 10.69 LANNVTHH160 pKa = 7.19 RR161 pKa = 11.84 VPLEE165 pKa = 4.05 DD166 pKa = 3.65 LHH168 pKa = 9.08 IEE170 pKa = 4.24 FKK172 pKa = 11.06 DD173 pKa = 4.1 CIVKK177 pKa = 10.54 SISLPGKK184 pKa = 10.01 KK185 pKa = 8.35 ITVHH189 pKa = 5.38 QFYY192 pKa = 11.39 SMIVNEE198 pKa = 4.32 VIKK201 pKa = 10.88 NKK203 pKa = 10.17 NYY205 pKa = 10.13 KK206 pKa = 9.98 HH207 pKa = 6.55 LFGPLDD213 pKa = 3.97 AFSHH217 pKa = 5.45 SQHH220 pKa = 5.79 FRR222 pKa = 11.84 DD223 pKa = 3.55 PAFATFFLDD232 pKa = 4.48 RR233 pKa = 11.84 IAMYY237 pKa = 9.33 EE238 pKa = 3.77 EE239 pKa = 4.53 RR240 pKa = 11.84 NPTFRR245 pKa = 11.84 SNLRR249 pKa = 11.84 DD250 pKa = 3.37 VFAAEE255 pKa = 4.28 IGCTIADD262 pKa = 3.91 KK263 pKa = 10.91 SVCSHH268 pKa = 7.07 DD269 pKa = 4.07 HH270 pKa = 5.78 LQHH273 pKa = 6.31 VIYY276 pKa = 10.65 SALDD280 pKa = 3.49 LLKK283 pKa = 10.53 NKK285 pKa = 9.8 HH286 pKa = 4.96 SSKK289 pKa = 10.9 VYY291 pKa = 9.37 VEE293 pKa = 4.16 TDD295 pKa = 4.2 FGCAIVSSTTIHH307 pKa = 6.34 FGKK310 pKa = 9.99 SCSPTTRR317 pKa = 11.84 LRR319 pKa = 11.84 TSEE322 pKa = 4.17 LASNLPAICEE332 pKa = 3.9 IVPIPFLHH340 pKa = 6.93 RR341 pKa = 11.84 SHH343 pKa = 6.25 ITAPEE348 pKa = 3.86 FLQILGSLNATDD360 pKa = 4.2 FDD362 pKa = 3.84 EE363 pKa = 4.96 FAFVINKK370 pKa = 9.75 RR371 pKa = 11.84 IRR373 pKa = 11.84 AHH375 pKa = 6.19 DD376 pKa = 3.39 THH378 pKa = 8.39 SEE380 pKa = 4.07 AKK382 pKa = 9.58 EE383 pKa = 3.75 HH384 pKa = 6.32 LTLSMKK390 pKa = 10.3 KK391 pKa = 10.06 KK392 pKa = 10.3 FVIEE396 pKa = 4.23 DD397 pKa = 3.87 FFPSRR402 pKa = 11.84 SNISLNEE409 pKa = 3.77 VKK411 pKa = 10.52 GASVLMNDD419 pKa = 3.49 SDD421 pKa = 4.31 CRR423 pKa = 11.84 IAIYY427 pKa = 10.25 SVIFHH432 pKa = 6.64 RR433 pKa = 11.84 EE434 pKa = 3.35 KK435 pKa = 10.97 HH436 pKa = 5.58 LVGTLSKK443 pKa = 10.6 KK444 pKa = 10.37 PEE446 pKa = 4.33 DD447 pKa = 3.73 SVVYY451 pKa = 8.42 YY452 pKa = 9.51 YY453 pKa = 10.41 PKK455 pKa = 10.93 VNATEE460 pKa = 4.22 CC461 pKa = 3.71
Molecular weight: 51.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.173
IPC2_protein 7.468
IPC_protein 7.293
Toseland 7.059
ProMoST 7.922
Dawson 7.995
Bjellqvist 8.419
Wikipedia 7.907
Rodwell 8.009
Grimsley 7.146
Solomon 8.039
Lehninger 8.053
Nozaki 8.668
DTASelect 8.141
Thurlkill 8.2
EMBOSS 8.214
Sillero 8.521
Patrickios 4.151
IPC_peptide 8.039
IPC2_peptide 7.556
IPC2.peptide.svr19 7.42
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3505
103
2592
701.0
79.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.191 ± 0.537
1.769 ± 0.509
6.391 ± 0.485
5.307 ± 0.613
5.05 ± 0.239
4.051 ± 0.466
3.252 ± 0.49
5.991 ± 0.345
5.364 ± 0.705
9.757 ± 1.333
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.854 ± 0.175
4.251 ± 0.379
5.193 ± 0.969
2.596 ± 0.324
5.735 ± 0.416
9.016 ± 0.631
5.592 ± 0.597
7.332 ± 0.278
0.656 ± 0.198
4.65 ± 0.327
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here