Mycobacterium phage Wachhund

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249XRT5|A0A249XRT5_9CAUD Cytosine-specific methyltransferase OS=Mycobacterium phage Wachhund OX=2027903 GN=SEA_WACHHUND_69 PE=3 SV=1
MM1 pKa = 7.71SDD3 pKa = 5.03CLLCDD8 pKa = 3.92HH9 pKa = 7.15PRR11 pKa = 11.84STHH14 pKa = 5.11TPQCRR19 pKa = 11.84TRR21 pKa = 11.84LGVDD25 pKa = 2.95ADD27 pKa = 3.66DD28 pKa = 3.59MTRR31 pKa = 11.84YY32 pKa = 6.71TQCLCPGFEE41 pKa = 4.04GTEE44 pKa = 4.05DD45 pKa = 3.75EE46 pKa = 6.37EE47 pKa = 4.58EE48 pKa = 4.31DD49 pKa = 3.42TWLDD53 pKa = 3.26

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249XRY8|A0A249XRY8_9CAUD Uncharacterized protein OS=Mycobacterium phage Wachhund OX=2027903 GN=SEA_WACHHUND_56 PE=4 SV=1
MM1 pKa = 7.8AGTAVLTPDD10 pKa = 5.37GIDD13 pKa = 3.45TLVTAAEE20 pKa = 3.98AAALCGVTTSTIYY33 pKa = 10.15VWVNRR38 pKa = 11.84GTLAPSGKK46 pKa = 9.55NRR48 pKa = 11.84TGHH51 pKa = 4.9NVYY54 pKa = 10.29RR55 pKa = 11.84VLDD58 pKa = 3.67VAKK61 pKa = 10.24AEE63 pKa = 3.99HH64 pKa = 5.91ATRR67 pKa = 11.84VKK69 pKa = 10.61ARR71 pKa = 11.84RR72 pKa = 11.84HH73 pKa = 4.39RR74 pKa = 3.77

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

17526

29

1182

173.5

18.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.773 ± 0.451

1.13 ± 0.148

6.659 ± 0.208

5.769 ± 0.301

2.887 ± 0.21

9.124 ± 0.586

2.271 ± 0.171

4.405 ± 0.219

3.172 ± 0.185

7.303 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.288 ± 0.15

3.304 ± 0.212

6.139 ± 0.203

3.418 ± 0.181

6.687 ± 0.387

5.871 ± 0.313

6.699 ± 0.329

7.206 ± 0.259

2.362 ± 0.13

2.533 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski