Amino acid dipepetide frequency for Mycobacterium phage Wachhund

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.265AlaAla: 14.265 ± 1.866
1.084AlaCys: 1.084 ± 0.284
7.304AlaAsp: 7.304 ± 0.796
7.76AlaGlu: 7.76 ± 0.689
3.252AlaPhe: 3.252 ± 0.499
11.013AlaGly: 11.013 ± 1.437
2.511AlaHis: 2.511 ± 0.338
4.108AlaIle: 4.108 ± 0.603
3.709AlaLys: 3.709 ± 0.426
7.475AlaLeu: 7.475 ± 0.735
2.796AlaMet: 2.796 ± 0.44
2.054AlaAsn: 2.054 ± 0.424
5.535AlaPro: 5.535 ± 0.674
3.424AlaGln: 3.424 ± 0.506
6.961AlaArg: 6.961 ± 0.602
5.364AlaSer: 5.364 ± 0.606
6.562AlaThr: 6.562 ± 0.727
7.304AlaVal: 7.304 ± 0.673
2.853AlaTrp: 2.853 ± 0.402
2.397AlaTyr: 2.397 ± 0.334
0.0AlaXaa: 0.0 ± 0.0
Cys
0.913CysAla: 0.913 ± 0.254
0.114CysCys: 0.114 ± 0.075
1.027CysAsp: 1.027 ± 0.27
0.799CysGlu: 0.799 ± 0.199
0.285CysPhe: 0.285 ± 0.119
1.598CysGly: 1.598 ± 0.386
0.228CysHis: 0.228 ± 0.116
0.057CysIle: 0.057 ± 0.06
0.342CysLys: 0.342 ± 0.144
0.628CysLeu: 0.628 ± 0.24
0.228CysMet: 0.228 ± 0.125
0.514CysAsn: 0.514 ± 0.162
1.084CysPro: 1.084 ± 0.26
0.456CysGln: 0.456 ± 0.178
0.856CysArg: 0.856 ± 0.23
0.571CysSer: 0.571 ± 0.195
0.685CysThr: 0.685 ± 0.191
0.571CysVal: 0.571 ± 0.171
0.285CysTrp: 0.285 ± 0.136
0.057CysTyr: 0.057 ± 0.051
0.0CysXaa: 0.0 ± 0.0
Asp
7.133AspAla: 7.133 ± 0.72
0.685AspCys: 0.685 ± 0.211
4.165AspAsp: 4.165 ± 0.576
3.31AspGlu: 3.31 ± 0.493
1.598AspPhe: 1.598 ± 0.261
6.79AspGly: 6.79 ± 0.69
1.427AspHis: 1.427 ± 0.257
2.282AspIle: 2.282 ± 0.377
1.484AspLys: 1.484 ± 0.245
6.049AspLeu: 6.049 ± 0.541
1.084AspMet: 1.084 ± 0.291
1.541AspAsn: 1.541 ± 0.349
4.964AspPro: 4.964 ± 0.638
2.568AspGln: 2.568 ± 0.326
5.364AspArg: 5.364 ± 0.638
3.595AspSer: 3.595 ± 0.65
4.964AspThr: 4.964 ± 0.404
3.994AspVal: 3.994 ± 0.494
1.94AspTrp: 1.94 ± 0.356
1.655AspTyr: 1.655 ± 0.287
0.0AspXaa: 0.0 ± 0.0
Glu
5.877GluAla: 5.877 ± 0.603
0.913GluCys: 0.913 ± 0.267
3.538GluAsp: 3.538 ± 0.416
2.568GluGlu: 2.568 ± 0.554
2.111GluPhe: 2.111 ± 0.418
3.367GluGly: 3.367 ± 0.486
1.255GluHis: 1.255 ± 0.271
2.111GluIle: 2.111 ± 0.389
1.94GluLys: 1.94 ± 0.28
5.078GluLeu: 5.078 ± 0.536
1.541GluMet: 1.541 ± 0.336
2.054GluAsn: 2.054 ± 0.279
2.625GluPro: 2.625 ± 0.414
2.91GluGln: 2.91 ± 0.415
5.364GluArg: 5.364 ± 0.572
3.252GluSer: 3.252 ± 0.542
3.937GluThr: 3.937 ± 0.599
3.88GluVal: 3.88 ± 0.426
1.312GluTrp: 1.312 ± 0.248
2.054GluTyr: 2.054 ± 0.412
0.0GluXaa: 0.0 ± 0.0
Phe
3.31PheAla: 3.31 ± 0.38
0.228PheCys: 0.228 ± 0.115
2.682PheAsp: 2.682 ± 0.431
1.769PheGlu: 1.769 ± 0.329
0.913PhePhe: 0.913 ± 0.324
2.454PheGly: 2.454 ± 0.512
0.285PheHis: 0.285 ± 0.117
1.198PheIle: 1.198 ± 0.351
0.856PheLys: 0.856 ± 0.212
1.826PheLeu: 1.826 ± 0.302
0.799PheMet: 0.799 ± 0.266
1.141PheAsn: 1.141 ± 0.286
1.541PhePro: 1.541 ± 0.295
0.913PheGln: 0.913 ± 0.299
1.598PheArg: 1.598 ± 0.241
1.769PheSer: 1.769 ± 0.267
2.111PheThr: 2.111 ± 0.355
2.111PheVal: 2.111 ± 0.33
0.628PheTrp: 0.628 ± 0.149
0.742PheTyr: 0.742 ± 0.284
0.0PheXaa: 0.0 ± 0.0
Gly
9.586GlyAla: 9.586 ± 1.331
1.027GlyCys: 1.027 ± 0.28
5.934GlyAsp: 5.934 ± 0.497
4.108GlyGlu: 4.108 ± 0.509
2.91GlyPhe: 2.91 ± 0.445
10.899GlyGly: 10.899 ± 3.066
2.282GlyHis: 2.282 ± 0.386
4.793GlyIle: 4.793 ± 0.655
2.739GlyLys: 2.739 ± 0.402
5.877GlyLeu: 5.877 ± 0.52
2.397GlyMet: 2.397 ± 0.425
3.367GlyAsn: 3.367 ± 0.574
4.451GlyPro: 4.451 ± 0.589
2.282GlyGln: 2.282 ± 0.573
4.793GlyArg: 4.793 ± 0.594
6.334GlySer: 6.334 ± 0.876
6.448GlyThr: 6.448 ± 0.702
5.934GlyVal: 5.934 ± 0.655
2.91GlyTrp: 2.91 ± 0.42
2.168GlyTyr: 2.168 ± 0.35
0.0GlyXaa: 0.0 ± 0.0
His
2.34HisAla: 2.34 ± 0.367
0.399HisCys: 0.399 ± 0.175
0.97HisAsp: 0.97 ± 0.194
1.141HisGlu: 1.141 ± 0.225
0.514HisPhe: 0.514 ± 0.157
1.94HisGly: 1.94 ± 0.27
1.027HisHis: 1.027 ± 0.312
1.712HisIle: 1.712 ± 0.362
0.742HisLys: 0.742 ± 0.221
1.541HisLeu: 1.541 ± 0.259
0.571HisMet: 0.571 ± 0.155
0.628HisAsn: 0.628 ± 0.174
1.484HisPro: 1.484 ± 0.273
1.027HisGln: 1.027 ± 0.188
1.769HisArg: 1.769 ± 0.346
0.799HisSer: 0.799 ± 0.193
1.769HisThr: 1.769 ± 0.334
1.369HisVal: 1.369 ± 0.309
0.285HisTrp: 0.285 ± 0.119
0.685HisTyr: 0.685 ± 0.152
0.0HisXaa: 0.0 ± 0.0
Ile
5.535IleAla: 5.535 ± 0.469
0.456IleCys: 0.456 ± 0.209
3.823IleAsp: 3.823 ± 0.448
3.595IleGlu: 3.595 ± 0.402
0.742IlePhe: 0.742 ± 0.262
3.88IleGly: 3.88 ± 0.519
1.598IleHis: 1.598 ± 0.345
1.427IleIle: 1.427 ± 0.256
1.027IleLys: 1.027 ± 0.256
2.34IleLeu: 2.34 ± 0.41
0.342IleMet: 0.342 ± 0.15
2.054IleAsn: 2.054 ± 0.313
2.511IlePro: 2.511 ± 0.367
1.369IleGln: 1.369 ± 0.273
2.796IleArg: 2.796 ± 0.426
1.598IleSer: 1.598 ± 0.355
3.994IleThr: 3.994 ± 0.496
3.252IleVal: 3.252 ± 0.373
1.027IleTrp: 1.027 ± 0.234
0.685IleTyr: 0.685 ± 0.188
0.0IleXaa: 0.0 ± 0.0
Lys
3.88LysAla: 3.88 ± 0.531
0.571LysCys: 0.571 ± 0.205
1.484LysAsp: 1.484 ± 0.265
1.598LysGlu: 1.598 ± 0.291
1.084LysPhe: 1.084 ± 0.206
2.454LysGly: 2.454 ± 0.369
1.312LysHis: 1.312 ± 0.261
1.141LysIle: 1.141 ± 0.276
1.084LysLys: 1.084 ± 0.338
2.168LysLeu: 2.168 ± 0.356
0.571LysMet: 0.571 ± 0.183
0.856LysAsn: 0.856 ± 0.244
2.111LysPro: 2.111 ± 0.341
1.541LysGln: 1.541 ± 0.292
1.826LysArg: 1.826 ± 0.282
1.655LysSer: 1.655 ± 0.265
1.883LysThr: 1.883 ± 0.334
2.739LysVal: 2.739 ± 0.421
0.514LysTrp: 0.514 ± 0.155
1.255LysTyr: 1.255 ± 0.261
0.0LysXaa: 0.0 ± 0.0
Leu
7.589LeuAla: 7.589 ± 0.755
0.799LeuCys: 0.799 ± 0.216
4.679LeuAsp: 4.679 ± 0.491
4.223LeuGlu: 4.223 ± 0.463
2.111LeuPhe: 2.111 ± 0.281
5.478LeuGly: 5.478 ± 0.63
1.027LeuHis: 1.027 ± 0.262
3.31LeuIle: 3.31 ± 0.434
2.397LeuLys: 2.397 ± 0.343
5.078LeuLeu: 5.078 ± 0.618
1.598LeuMet: 1.598 ± 0.335
2.34LeuAsn: 2.34 ± 0.454
5.706LeuPro: 5.706 ± 0.691
2.568LeuGln: 2.568 ± 0.415
5.078LeuArg: 5.078 ± 0.624
5.763LeuSer: 5.763 ± 0.489
5.535LeuThr: 5.535 ± 0.616
4.451LeuVal: 4.451 ± 0.56
1.198LeuTrp: 1.198 ± 0.256
2.111LeuTyr: 2.111 ± 0.377
0.0LeuXaa: 0.0 ± 0.0
Met
2.111MetAla: 2.111 ± 0.378
0.285MetCys: 0.285 ± 0.175
1.484MetAsp: 1.484 ± 0.294
0.799MetGlu: 0.799 ± 0.166
0.685MetPhe: 0.685 ± 0.197
1.883MetGly: 1.883 ± 0.319
0.285MetHis: 0.285 ± 0.139
0.856MetIle: 0.856 ± 0.23
0.856MetLys: 0.856 ± 0.221
1.997MetLeu: 1.997 ± 0.289
0.742MetMet: 0.742 ± 0.253
1.141MetAsn: 1.141 ± 0.237
1.369MetPro: 1.369 ± 0.332
0.228MetGln: 0.228 ± 0.104
1.312MetArg: 1.312 ± 0.288
2.853MetSer: 2.853 ± 0.401
1.997MetThr: 1.997 ± 0.298
1.312MetVal: 1.312 ± 0.354
0.285MetTrp: 0.285 ± 0.116
0.399MetTyr: 0.399 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
3.195AsnAla: 3.195 ± 0.407
0.171AsnCys: 0.171 ± 0.111
2.225AsnAsp: 2.225 ± 0.428
1.369AsnGlu: 1.369 ± 0.333
0.913AsnPhe: 0.913 ± 0.285
3.88AsnGly: 3.88 ± 0.515
0.97AsnHis: 0.97 ± 0.203
1.598AsnIle: 1.598 ± 0.414
0.913AsnLys: 0.913 ± 0.224
2.111AsnLeu: 2.111 ± 0.36
0.685AsnMet: 0.685 ± 0.171
1.769AsnAsn: 1.769 ± 0.388
2.796AsnPro: 2.796 ± 0.363
1.027AsnGln: 1.027 ± 0.335
1.997AsnArg: 1.997 ± 0.352
1.712AsnSer: 1.712 ± 0.369
2.397AsnThr: 2.397 ± 0.325
1.769AsnVal: 1.769 ± 0.351
0.742AsnTrp: 0.742 ± 0.165
0.799AsnTyr: 0.799 ± 0.215
0.0AsnXaa: 0.0 ± 0.0
Pro
5.706ProAla: 5.706 ± 0.743
0.799ProCys: 0.799 ± 0.244
4.565ProAsp: 4.565 ± 0.551
4.394ProGlu: 4.394 ± 0.571
1.598ProPhe: 1.598 ± 0.306
6.733ProGly: 6.733 ± 0.648
1.427ProHis: 1.427 ± 0.265
1.94ProIle: 1.94 ± 0.328
2.168ProLys: 2.168 ± 0.383
4.165ProLeu: 4.165 ± 0.489
1.369ProMet: 1.369 ± 0.335
2.682ProAsn: 2.682 ± 0.448
3.88ProPro: 3.88 ± 0.53
1.826ProGln: 1.826 ± 0.319
3.823ProArg: 3.823 ± 0.518
3.709ProSer: 3.709 ± 0.468
3.424ProThr: 3.424 ± 0.534
4.679ProVal: 4.679 ± 0.452
0.97ProTrp: 0.97 ± 0.263
1.541ProTyr: 1.541 ± 0.279
0.0ProXaa: 0.0 ± 0.0
Gln
4.736GlnAla: 4.736 ± 0.386
0.399GlnCys: 0.399 ± 0.157
1.427GlnAsp: 1.427 ± 0.275
1.541GlnGlu: 1.541 ± 0.401
1.141GlnPhe: 1.141 ± 0.267
2.168GlnGly: 2.168 ± 0.491
0.742GlnHis: 0.742 ± 0.248
1.997GlnIle: 1.997 ± 0.31
1.027GlnLys: 1.027 ± 0.217
2.853GlnLeu: 2.853 ± 0.4
0.456GlnMet: 0.456 ± 0.141
0.799GlnAsn: 0.799 ± 0.251
2.739GlnPro: 2.739 ± 0.428
1.598GlnGln: 1.598 ± 0.323
2.511GlnArg: 2.511 ± 0.391
2.168GlnSer: 2.168 ± 0.374
1.997GlnThr: 1.997 ± 0.373
2.225GlnVal: 2.225 ± 0.296
0.742GlnTrp: 0.742 ± 0.189
0.913GlnTyr: 0.913 ± 0.282
0.0GlnXaa: 0.0 ± 0.0
Arg
6.163ArgAla: 6.163 ± 0.632
1.084ArgCys: 1.084 ± 0.329
4.622ArgAsp: 4.622 ± 0.507
4.622ArgGlu: 4.622 ± 0.686
1.94ArgPhe: 1.94 ± 0.3
4.451ArgGly: 4.451 ± 0.418
1.427ArgHis: 1.427 ± 0.319
3.766ArgIle: 3.766 ± 0.495
2.168ArgLys: 2.168 ± 0.33
5.25ArgLeu: 5.25 ± 0.621
2.397ArgMet: 2.397 ± 0.408
2.568ArgAsn: 2.568 ± 0.472
3.252ArgPro: 3.252 ± 0.438
1.94ArgGln: 1.94 ± 0.334
5.535ArgArg: 5.535 ± 0.903
3.424ArgSer: 3.424 ± 0.387
3.081ArgThr: 3.081 ± 0.528
5.193ArgVal: 5.193 ± 0.637
2.054ArgTrp: 2.054 ± 0.361
1.94ArgTyr: 1.94 ± 0.312
0.0ArgXaa: 0.0 ± 0.0
Ser
6.334SerAla: 6.334 ± 0.882
0.456SerCys: 0.456 ± 0.161
4.28SerAsp: 4.28 ± 0.529
2.91SerGlu: 2.91 ± 0.51
2.225SerPhe: 2.225 ± 0.465
6.562SerGly: 6.562 ± 0.752
1.141SerHis: 1.141 ± 0.231
2.682SerIle: 2.682 ± 0.476
2.225SerLys: 2.225 ± 0.324
3.709SerLeu: 3.709 ± 0.523
1.427SerMet: 1.427 ± 0.286
1.826SerAsn: 1.826 ± 0.367
3.481SerPro: 3.481 ± 0.373
1.94SerGln: 1.94 ± 0.355
3.367SerArg: 3.367 ± 0.41
3.652SerSer: 3.652 ± 0.735
3.31SerThr: 3.31 ± 0.459
4.565SerVal: 4.565 ± 0.58
1.312SerTrp: 1.312 ± 0.246
1.312SerTyr: 1.312 ± 0.228
0.0SerXaa: 0.0 ± 0.0
Thr
6.391ThrAla: 6.391 ± 0.583
0.628ThrCys: 0.628 ± 0.212
3.994ThrAsp: 3.994 ± 0.513
3.595ThrGlu: 3.595 ± 0.413
1.826ThrPhe: 1.826 ± 0.341
6.733ThrGly: 6.733 ± 0.825
1.712ThrHis: 1.712 ± 0.298
3.709ThrIle: 3.709 ± 0.492
1.94ThrLys: 1.94 ± 0.408
4.679ThrLeu: 4.679 ± 0.495
1.369ThrMet: 1.369 ± 0.3
2.397ThrAsn: 2.397 ± 0.354
4.622ThrPro: 4.622 ± 0.659
1.997ThrGln: 1.997 ± 0.291
3.709ThrArg: 3.709 ± 0.437
3.538ThrSer: 3.538 ± 0.467
5.021ThrThr: 5.021 ± 0.646
5.877ThrVal: 5.877 ± 0.728
1.198ThrTrp: 1.198 ± 0.3
1.997ThrTyr: 1.997 ± 0.35
0.0ThrXaa: 0.0 ± 0.0
Val
7.703ValAla: 7.703 ± 0.557
0.913ValCys: 0.913 ± 0.235
5.078ValAsp: 5.078 ± 0.511
4.337ValGlu: 4.337 ± 0.523
1.883ValPhe: 1.883 ± 0.369
5.706ValGly: 5.706 ± 0.534
1.084ValHis: 1.084 ± 0.265
2.853ValIle: 2.853 ± 0.511
2.168ValLys: 2.168 ± 0.37
5.877ValLeu: 5.877 ± 0.655
1.484ValMet: 1.484 ± 0.228
1.997ValAsn: 1.997 ± 0.347
4.337ValPro: 4.337 ± 0.432
2.796ValGln: 2.796 ± 0.403
4.508ValArg: 4.508 ± 0.621
4.679ValSer: 4.679 ± 0.54
4.85ValThr: 4.85 ± 0.543
6.505ValVal: 6.505 ± 0.645
1.769ValTrp: 1.769 ± 0.382
1.541ValTyr: 1.541 ± 0.292
0.0ValXaa: 0.0 ± 0.0
Trp
1.997TrpAla: 1.997 ± 0.308
0.057TrpCys: 0.057 ± 0.051
1.655TrpAsp: 1.655 ± 0.378
1.541TrpGlu: 1.541 ± 0.339
0.628TrpPhe: 0.628 ± 0.178
1.084TrpGly: 1.084 ± 0.291
0.628TrpHis: 0.628 ± 0.184
1.255TrpIle: 1.255 ± 0.236
1.141TrpLys: 1.141 ± 0.213
2.168TrpLeu: 2.168 ± 0.376
0.742TrpMet: 0.742 ± 0.208
0.628TrpAsn: 0.628 ± 0.224
1.198TrpPro: 1.198 ± 0.298
1.027TrpGln: 1.027 ± 0.306
1.997TrpArg: 1.997 ± 0.502
1.312TrpSer: 1.312 ± 0.332
1.312TrpThr: 1.312 ± 0.281
1.769TrpVal: 1.769 ± 0.404
1.027TrpTrp: 1.027 ± 0.271
0.456TrpTyr: 0.456 ± 0.171
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.967TyrAla: 2.967 ± 0.444
0.228TyrCys: 0.228 ± 0.114
1.655TyrAsp: 1.655 ± 0.368
1.712TyrGlu: 1.712 ± 0.34
0.514TyrPhe: 0.514 ± 0.183
2.168TyrGly: 2.168 ± 0.357
0.342TyrHis: 0.342 ± 0.126
1.255TyrIle: 1.255 ± 0.226
0.799TyrLys: 0.799 ± 0.208
2.168TyrLeu: 2.168 ± 0.331
0.228TyrMet: 0.228 ± 0.107
0.685TyrAsn: 0.685 ± 0.185
1.712TyrPro: 1.712 ± 0.273
0.742TyrGln: 0.742 ± 0.196
1.712TyrArg: 1.712 ± 0.305
0.97TyrSer: 0.97 ± 0.247
1.712TyrThr: 1.712 ± 0.346
2.568TyrVal: 2.568 ± 0.325
0.571TyrTrp: 0.571 ± 0.167
0.628TyrTyr: 0.628 ± 0.201
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 101 proteins (17526 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski