Erwinia sp. 198

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Erwinia; unclassified Erwinia

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4064 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A427K4L0|A0A427K4L0_9GAMM Uncharacterized protein OS=Erwinia sp. 198 OX=2022746 GN=EGK14_16755 PE=4 SV=1
MM1 pKa = 8.18DD2 pKa = 4.97SNIEE6 pKa = 4.15KK7 pKa = 9.26TCEE10 pKa = 3.77LDD12 pKa = 3.41NLVIIYY18 pKa = 8.95FGQDD22 pKa = 2.48CDD24 pKa = 5.06IFDD27 pKa = 4.47EE28 pKa = 4.82EE29 pKa = 5.34CDD31 pKa = 3.93FDD33 pKa = 4.73NLLNEE38 pKa = 4.38YY39 pKa = 10.37LNTSSEE45 pKa = 3.87FSLRR49 pKa = 11.84MLLANLIEE57 pKa = 5.25LNAQNDD63 pKa = 3.75RR64 pKa = 11.84CEE66 pKa = 4.28VLLSRR71 pKa = 11.84YY72 pKa = 9.49NGEE75 pKa = 4.01FAPDD79 pKa = 3.34RR80 pKa = 11.84WEE82 pKa = 3.8MTAQEE87 pKa = 4.16WLDD90 pKa = 3.27IVNRR94 pKa = 11.84RR95 pKa = 11.84LVDD98 pKa = 3.22YY99 pKa = 9.51MNEE102 pKa = 3.92KK103 pKa = 10.56GYY105 pKa = 8.49STEE108 pKa = 3.57LSYY111 pKa = 11.18FF112 pKa = 3.54

Molecular weight: 13.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R8YRZ6|A0A3R8YRZ6_9GAMM Haloacid dehalogenase type II OS=Erwinia sp. 198 OX=2022746 GN=EGK14_20590 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84SRR41 pKa = 11.84LTVSKK46 pKa = 11.03

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4064

0

4064

1278030

15

3142

314.5

34.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.382 ± 0.046

1.012 ± 0.012

5.097 ± 0.029

5.637 ± 0.037

3.827 ± 0.028

7.493 ± 0.047

2.2 ± 0.019

5.447 ± 0.035

4.148 ± 0.032

11.095 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.654 ± 0.017

3.555 ± 0.029

4.486 ± 0.027

4.613 ± 0.035

5.92 ± 0.034

5.999 ± 0.031

5.302 ± 0.028

6.904 ± 0.031

1.512 ± 0.017

2.715 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski