Acrocarpospora pleiomorpha
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10405 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5M3XNG9|A0A5M3XNG9_9ACTN Maltose/maltodextrin transport system permease protein OS=Acrocarpospora pleiomorpha OX=90975 GN=Aple_056630 PE=3 SV=1
MM1 pKa = 7.35 SALRR5 pKa = 11.84 SAAVVAAVLIFSTTIAVAPAYY26 pKa = 10.33 AIDD29 pKa = 3.62 TNAWVQGGEE38 pKa = 4.18 LNYY41 pKa = 9.5 TAAPGQVNHH50 pKa = 5.92 VVFTRR55 pKa = 11.84 TDD57 pKa = 3.18 ANTYY61 pKa = 10.45 LVDD64 pKa = 5.56 DD65 pKa = 4.93 IVAITPLEE73 pKa = 4.73 GCTHH77 pKa = 6.79 PNARR81 pKa = 11.84 DD82 pKa = 3.43 LTLVQCTAEE91 pKa = 4.29 FGFGGQLIVDD101 pKa = 4.18 VGDD104 pKa = 4.49 LDD106 pKa = 6.36 DD107 pKa = 4.61 IVNPTGPNHH116 pKa = 6.7 ALIGLGSGDD125 pKa = 4.18 DD126 pKa = 3.34 IAYY129 pKa = 8.7 GFPGSVGSLVDD140 pKa = 4.06 GGPGDD145 pKa = 4.99 DD146 pKa = 5.56 LIHH149 pKa = 6.7 SGPGQHH155 pKa = 7.09 LIDD158 pKa = 5.03 GGTGTDD164 pKa = 3.32 TVSYY168 pKa = 9.1 AARR171 pKa = 11.84 VAPVTVDD178 pKa = 5.11 LAACPCGEE186 pKa = 4.38 AGEE189 pKa = 5.02 ADD191 pKa = 3.66 TLNGVEE197 pKa = 4.05 NVIGGSASDD206 pKa = 3.76 TLTGDD211 pKa = 3.55 TLANTLTGGGGNDD224 pKa = 3.46 VIAGKK229 pKa = 10.44 SGADD233 pKa = 3.49 TLNGGIGTDD242 pKa = 3.58 TLKK245 pKa = 11.21 GGWGNDD251 pKa = 3.41 TLNGGLGPDD260 pKa = 3.85 TLTGGWGNDD269 pKa = 3.23 TLTGGLGNDD278 pKa = 3.78 TLRR281 pKa = 11.84 GGSGNDD287 pKa = 3.46 TLNGGVGFADD297 pKa = 4.2 DD298 pKa = 4.33 CNGGLGADD306 pKa = 3.71 VEE308 pKa = 4.8 IGCEE312 pKa = 3.82 LL313 pKa = 3.63
Molecular weight: 30.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.567
IPC_protein 3.617
Toseland 3.376
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.605
Rodwell 3.439
Grimsley 3.274
Solomon 3.617
Lehninger 3.579
Nozaki 3.745
DTASelect 4.05
Thurlkill 3.439
EMBOSS 3.617
Sillero 3.745
Patrickios 0.629
IPC_peptide 3.605
IPC2_peptide 3.706
IPC2.peptide.svr19 3.675
Protein with the highest isoelectric point:
>tr|A0A5M3Y297|A0A5M3Y297_9ACTN NADH-quinone oxidoreductase subunit I OS=Acrocarpospora pleiomorpha OX=90975 GN=nuoI2 PE=3 SV=1
MM1 pKa = 6.9 TAAKK5 pKa = 10.21 AGVRR9 pKa = 11.84 TVSGRR14 pKa = 11.84 IAAGKK19 pKa = 9.68 AAGTKK24 pKa = 10.06 KK25 pKa = 10.37 SVAAGRR31 pKa = 11.84 AATKK35 pKa = 9.46 TAMAKK40 pKa = 9.02 TAVAKK45 pKa = 10.43 KK46 pKa = 10.27 AVVKK50 pKa = 10.65 KK51 pKa = 10.49 AVAKK55 pKa = 8.94 TAAGKK60 pKa = 9.72 AAAKK64 pKa = 9.75 KK65 pKa = 9.52 AAAGGRR71 pKa = 11.84 RR72 pKa = 11.84 SVGSGGRR79 pKa = 11.84 SS80 pKa = 2.98
Molecular weight: 7.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.446
IPC2_protein 11.052
IPC_protein 12.544
Toseland 12.735
ProMoST 13.203
Dawson 12.749
Bjellqvist 12.72
Wikipedia 13.188
Rodwell 12.778
Grimsley 12.778
Solomon 13.217
Lehninger 13.115
Nozaki 12.735
DTASelect 12.72
Thurlkill 12.735
EMBOSS 13.232
Sillero 12.735
Patrickios 12.501
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 8.971
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10405
0
10405
3444316
39
9382
331.0
35.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.904 ± 0.037
0.776 ± 0.007
5.834 ± 0.02
5.476 ± 0.024
2.956 ± 0.013
9.149 ± 0.024
2.206 ± 0.012
4.068 ± 0.016
2.043 ± 0.017
10.53 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.881 ± 0.011
2.054 ± 0.017
5.948 ± 0.021
2.842 ± 0.013
7.829 ± 0.028
5.241 ± 0.018
6.054 ± 0.028
8.455 ± 0.024
1.594 ± 0.01
2.16 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here